NZ523385A - Biodegradable vehicles and BAS-loaded delivery systems for use as biodegradable fillers and/or spacers, e.g. artificial skin - Google Patents
Biodegradable vehicles and BAS-loaded delivery systems for use as biodegradable fillers and/or spacers, e.g. artificial skinInfo
- Publication number
- NZ523385A NZ523385A NZ523385A NZ52338501A NZ523385A NZ 523385 A NZ523385 A NZ 523385A NZ 523385 A NZ523385 A NZ 523385A NZ 52338501 A NZ52338501 A NZ 52338501A NZ 523385 A NZ523385 A NZ 523385A
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- NZ
- New Zealand
- Prior art keywords
- agents
- glyceryl
- biodegradable
- biologically active
- peg
- Prior art date
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/34—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0024—Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1641—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poloxamers
- A61K9/1647—Polyesters, e.g. poly(lactide-co-glycolide)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1682—Processes
- A61K9/1694—Processes resulting in granules or microspheres of the matrix type containing more than 5% of excipient
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
- A61L31/041—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/141—Plasticizers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/148—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
- C08K5/00—Use of organic ingredients
- C08K5/04—Oxygen-containing compounds
- C08K5/10—Esters; Ether-esters
- C08K5/11—Esters; Ether-esters of acyclic polycarboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/14—Esters of carboxylic acids, e.g. fatty acid monoglycerides, medium-chain triglycerides, parabens or PEG fatty acid esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Heart & Thoracic Surgery (AREA)
- Surgery (AREA)
- Vascular Medicine (AREA)
- Dermatology (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Neurosurgery (AREA)
- Molecular Biology (AREA)
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Biodegradable vehicle and delivery systems of physiologically, pharmacologically and biologically active substances (BAS) are provided. The biodegradable vehicles may be prepared by blending biodegradable polymers and plasticizers using a solvent evaporation method. This method involves dissolving the biodegradable polymer or copolymer and a plasticizer or mixtures of plasticizers into a volatile solvent or mixtures of volatile solvents. The volatile solvent is then removed using vacuum or at an elevated temperature or using a combination of both vacuum and elevated temperature. The biodegradable vehicle can be used as filler or spacer in the body. Biologically active substances (BAS) can be added to the biodegradable vehicle at any step during or after preparing the biodegradable vehicle, or just prior to using the biodegradable delivery system. This biodegradable delivery system provides controlled release of the BAS over the desired period of time. The biodegradable vehicle or BAS-loaded biodegradable delivery system can be injected, implanted, smeared or applied in vivo in an animal, bird or human.
Description
52
U
PCT/USO1/06138
BIODEGRADABLE VEHICLES AND DELIVERY SYSTEMS OF BIOLOGICALLY ACTIVE SUBSTANCES
CROSS-REFERENCE TO RELATED APPLICATIONS
This application claims priority to U.S. Patent Application 09/605,661, filed 5 June 28,2000, the teachings of which are incorporated herein by reference in their entirety for all purposes.
FIELD OF THE INVENTION
Biodegradable vehicles and delivery systems, which can be mixed with one or 10 more physiologically, pharmacologically and biologically active substance(s) (BAS), are provided. The biodegradable vehicle (without any BAS-loading) can be used as a biodegradable filler or spacer to fill in cavities or body tissues in animals, birds and humans. The biodegradable vehicle can be mixed with one or more BAS. The delivery systems loaded with BAS can be used to control the release of the BAS from the delivery system for a 15 prolonged period of time. The consistency and rheology, hydrophilicity and hydrophobicity, and in vivo degradation rates of the biodegradable vehicles and BAS loaded delivery systems are controlled by modulating the types of polymers or copolymers, molecular weight of polymers and copolymers, copolymer ratios, and ratios of blends of polymers or copolymers with different molecular weights or different hydrophilicity or hydrophobicity, types of 20 .plasticizers, concentration of plasticizers, ratios of two or more plasticizers used in _ combination. The release characteristics of the BAS from the biodegradable delivery system
™ are also controlled by the above-mentioned factors. The present invention also provides methods for preparing these biodegradable vehicles and delivery systems.
BACKGROUND OF THE INVENTION
The term biodegradable polymers refer to those polymers, which are slowly converted to nontoxic degradation products in the body. Examples include homopolymers and copolymers of polylactic acid or polylactide (PLA), polyglycolic acid or polyglycolide,
polycaprolactone (PCL), polyanhydrides, polyphosphoesters, polyorfhoesters,
polyaminoacids, pseudopolyaminoacids, polyhydroxybutyrates, polyhydroxyvalerates,
polyphosphazenes, polyalkylcyanoacrylates, polydioxanone, poly(s-decaloactone),
I
poly(glycolide-co-trimethylene carbonate), poly(ethylene carbonate), poly(iminocarbonate), poly(l,3-propylene malonate), poly(ethylene-l,4-phenylene-bis-oxyacetate), poly(ester-amides). Some of these polymers and their copolymers have been studied extensively for biomedical applications such as sutures, staples and mesh for wound closure, fracture 5 fixation, bone augmentation and ligament reconstruction in orthopedics, ligation clips and vascular grafts in cardiovascular surgery, and dental repairs (Barrows T. Degradable implant materials: a review of synthetic absorbable polymers and their applications. Clinical materials., 1:233-257,1986). They have also been used to prepare biodegradable drug delivery systems capable of releasing the drug or a biologically active substance over the 10 desired length of time.
The advantages of using biodegradable polymers in biodegradable delivery systems of BAS are: ready availability of polymers, polymers used are nontoxic, biocompatibile and biodegradable, facile predictability of biodegradation rates of the polymers, ease of modification of the degradation characteristics of the polymers, regulatory 15 approval of some of the commonly used biodegradable polymers, ease of fabrication of the polymers into various types of devices and the possibility of controlling the release of BAS by polymers over the desired length of time.
Release of BAS from a polymeric delivery system depends on the physicochemical characteristics of the BAS molecule, polymer and other excipients, and the 20 dosage form. The important factors governing BAS release characteristics from the delivery systems prepared with biodegradable polymers are polymer molecular weight, copolymer ratio, polymer hydrophilicity or lipophilicity, percentage of various polymers in a blend consisting of polymers with varying molecular weights or copolymer ratios, hydrophilicity or hydrophilicity of the platicizer, percentage of various hydrophilic and hydrophilic plasticizers 25 in a blend of varying types of plasticizers, degree of plasticization, particle size and percentage of BAS-loading, hydrophilicity or lipophilicity of the incorporated BAS,
solubility of the BAS in both the delivery system and in the biological fluids, physical form of the formulation (i.e. liquid, gel or paste), and the method of preparation of the delivery system.
Several types of BAS delivery systems have been prepared from biodegradable polymers. These include microparticles such as microspheres and microcapsules (Schindler A, Jeffcoat R, Kimmel GL, Pitt CG, Wall ME and Zwelinger R., in:
Contemporary Topics in Polymer Science, Pearce EM and Schaefgen JR, eds., Vol. 2,
Plenum Publishing Corporation, New York, pp. 251-289,1977; Mason NS, Gupta DVS,
}r
Keller, DW, Youngquist RS, and Sparks RF. Biomedical applications of microencapsulation, (Lim F, ed.), CRC Press Inc., Florida, pp. 75-84,1984; Harrigan SE, McCarthy DA, Reuning R and Thies C., Midi. Macromol. Monograph, 5:91-100,1978.; Sanders LM, Burns R, Bitale K and Hoffman P., Clinical performance of nafarelin controlled release injectable: influence 5 of formulation parameters on release kinetics and duration of efficacy., Proceedings of the
International Symposium on Controlled Release and Bioactive Materials, 15:62-63,1988;
*
Mathiowitz E, Leong K and Langer R., Macromolecular drug release from bioerodible polyanhydride microspheres, in: Proceedings of the 12th International Symposium on Controlled Release of Bioactive Materials, Peppas N and Haluska R, eds., pp. 183,1985), 10 films (Jackanicz TM, Nash HA, Wise DL and Gregory JB. Polylactic acid as a biodegradable carrier for contraceptive steroids., Contraception, 8:227-233,1973.; Woodland JHR, Yolles S, Blake AB, Helrich M and Meyer FJ. Long-acting delivery systems for narcotic antagonist. I. J. Med. Chem., 16:897-901,1973), fibers (EeninkMJD, MaassenGCT, Sam AP, Geelen JAA, van Lieshout JB JM, Olijslager J, de Nijs H, and de Jager E. Development of a new 15 long-acting contraceptive subdermal implant releasing 3-ketodesogeatrel., Proceedings of the 15th International Symposium on Controlled Release of Bioactive Materials, Controlled Release Society, Lincolnshire, Illinois, pp.402-403,1988), capsules (Sidman KR, Schwope AD, Steber WD, Rudolph SE, Paulin SB. Biodegradable, implantable sustained release systems based on glutamic acid copolymers. J. Membr. Sci., 7:277-291,1980; Pitt CG, 20 Gratzl MM, Jeffcoat MA, Zweidinger R and Schindler A. Sustained drug delivery systems II: Factors affecting release rates from poly-s-caprolactone and related biodegradable polyesters., J. Pharm. Sci., 68(12): 1534-1538,1979), discs (Cowsar DR, DunnRL., Biodegradable and non-biodegradable fibrous delivery systems, in: Long acting Contraceptive Delivery Systems, Zatuchni GI, Goldsmith A, Shelton JD and Sciarra JJ, eds., 25 Harper & Row, Publishers, Philadelphia, pp. 145-148,1984), wafers (Brem et al., J. Neurosurgery, 74:441-446,1991) and solutions (Dunn et al., U.S. Patents 4,938,763; 5,324,519; 5,324,520; 5,278,201; 5,340,849; 5,368,859; 5,660849; 5,632,727; 5,599,552; 5,487,897). All of these, with the exception of microparticles need to be surgically implanted. This procedure is inconvenient and undesirable. Drag-loaded microspheres on 30 the other hand, can be easily injected. However, there are several inherent disadvantages of microparticles. These include the need for reconstitution before injection, the inability to remove the dose once it is injected, and the relatively complicated manufacturing procedure.
In addition, all the drug delivery systems described in the aforementioned section contains at least one BAS, which is incorporated into the drug delivery system during the manufacturing of the dosage form. It is often difficult (if not impossible) to individualize BAS dosing (or change the BAS-loading) in these drug delivery systems. Also, there exists a 5 possibility where a certain percentage of BAS often degrades because of its exposure to the solvents, chemicals or other harsh manufacturing conditions during the preparation of the drug delivery system or during storage of the finished product.
Therefore, there clearly exists a need for developing easily injectable,
implantable, smearable or applicable biodegradable vehicles and BAS-loaded biodegradable 10 delivery systems such as free-flowing or viscous liquids, gels, and pastes, prepared from biodegradable polymers using alternative methods. Moreover, there is also a need for developing a more versatile delivery vehicle where the type of BAS and the dose of BAS can be tailored (to individualize BAS dosing) just prior to its use. The stability of the BAS can also be enhanced in such a delivery system where the BAS is loaded into the vehicle just 15 prior to use.
It is an object of the present invention to go some way towards meeting these needs and/or to provide the public with a useful choice.
SUMMARY OF THE INVENTION
In certain aspects, the present invention relates to compositions and methods of preparing biodegradable vehicles and delivery systems. The present invention also 20 provides compositions of biodegradable vehicles and BAS-loaded delivery systems, and the process of blending one or more BAS with the biodegradable vehicles. The biodegradable vehicles can be used as biodegradable fillers or spacers (e.g., an artificial tissue) to fill in cavities or body tissues in animals, birds and humans. One or more biologically active substances (BAS) can be loaded into the biodegradable vehicle to prepare the biodegradable 25 delivery system, which can be used to control the release of the BAS over a desired period of time.
In one aspect, the present invention provides a biodegradable vehicle or delivery system comprising:
(a) at least one biodegradable polymer, said polymer selected from the group 30 consisting of homopolymers and copolymers or blends thereof, of polyesters, polyphosphoesters, polyorthoesters, polylactic acid or polylactides, polyglycolic acid or polyglycolides, polycaprolactones, polyalkylcyanoacrylates, polyphosphazenes, polyhydroxybutyrates, polyhydroxyvalerates, polyaminoacids, pseudopolyamino acids, polyamides, polyanhydrides, polydioxanone, poly(e-decaloactone), poly(glycolide-co-trimethylene carbonate), poly (ethylene carbonate), poly (iminocarbonate), poly(l, 3-propylene malonate), poly (ethylene-1,4-phenylene-bis- oxyacetate), and poly (ester-amides); and
4
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(b) at least one plasticizer; said plasticizer being capable of modulating both the consistency and hydrophobicity or hydrophilicity of said biodegradable vehicle or delivery system.
Optionally, the biodegradable vehicle or delivery system may further comprise:
(c) at least one biologically active substance.
Preferably, the plasticizer is capable of modulating the consistency, the hydrophobicity, hydrophilicity and degradation characteristics of the biodegradable vehicle.
The biodegradable polymer or blends thereof is/are capable of modulating the degradation kinetics of the biodegradable vehicle and in certain instances, the consistency, the hydrophobicity and the hydrophilicity of the biodegradable vehicle as well. The plasticizer or blends thereof are also capable of modulating the degradation kinetics, the consistiency, the hydrophilicity and the hydrophobicity of the biodegradable vehicle as well.
In another aspect, the present invention provides a biodegradable vehicle or delivery system comprising: (a) at least one biodegradable polymer, the polymer selected from polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones,
polyhydroxybutyrates, polyhydroxyvalerates, polyamides and polyanhydrides; and (b) at least two plasticizers, one of the plasticizers being hydrophilic and the other of the plasticizers being hydrophobic; and .(c) at least one biologically active substance.
In another aspect, the invention provides a method for the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolmers of polyesters, polyorhoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
In another aspect, the invention provides a method for enhancing the stability of the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or polymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, porno acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
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In another aspect the invention provides a method for modulating the release characteristics of the biologically active substance in the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
In another aspect the invention provides a method for modulating the dose of a bioactive agent in the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biological active substance-loaded biodegradable vehicle or delivery system.
In another aspect the invention provides a method for modulating the consistency or rheology of the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates; polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
In another aspect the invention provides a method for modulating the hydrophilicity or hydrophobicity of the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group costing of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
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(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
In another aspect the invention provides a method for modulating the degradation of the formulation of a biodegradable vehicle or delivery system comprising mixing:
(a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides;
(b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and
(c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
The method of manufacturing the biodegradable vehicles described in the present invention involves dissolving one or more biodegradable polymers and one or more plasticizers in a volatile solvent or mixture of volatile solvents. The volatile solvent or mixture of volatile solvents is/are then removed using vacuum or evaporated at an elevated temperature, or removed using both vacuum and elevated temperature. The resulting biodegradable vehicles can be free flowing or viscous liquids, gels or pastes. This method is particularly suited when polymers of high molecular weights are used to prepare the vehicles or BAS delivery system, or when a high consistency of the biodegradable vehicle or BAS delivery system, is desired. Alternatively, one or more biodegradable polymers can be directly dissolved in one or more plasticizers by stirring the mixture with or without the use of heat. This method is particularly suited when polymers of low molecular weights are used to prepare the biodegradable vehicles or BAS delivery system, or when a low consistency or BAS delivery system is desired.
In order to prepare a BAS-loaded delivery system, the BAS can be loaded into the biodegradable vehicle in any physical form (i.e. solid, liquid, gel or paste, where the BAS is dissolved or suspended in the plasticizer or mixtures of plasticizers, volatile solvents or mixture of volatile solvents or mixtures of volatile solvents and plasticizers) at any step during the manufacturing process of biodegradable delivery systems before the volatile solvent is completely removed. The BAS-loaded delivery system can also be manufactured by loading the BAS soon after the biodegradable vehicle is prepared, or blending the BAS to the biodegradable vehicle just prior to the use of the BAS-loaded biodegradable delivery system. Mixing of the BAS with the biodegradable vehicle can be accomplished by simply stirring the mixture with a stirring device, or by triturating the mixture or employing an ointment mill or a suitable device or apparatus or equipment that can be used for blending/mixing. When the BAS is blended with the biodegradable vehicle just prior to use, it
5a
377107 l.DOC
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could be stored in a separate container in a solid state, liquid state (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers), or gel or paste (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers). Alternatively, a device, which resembles two syringes or syringe-like devices (e.g. pumps in which materials 5 can be mixed by depressing a trigger-like device) attached together with a removable partition or a valve assembly can also be used to uniformly mix the BAS with the biodegradable vehicle. The BAS is loaded in one syringe or compartment and the biodegradable vehicle is loaded in the other compartment. A removable partition or a valve, which will allow the contents of the two compartments to be mixed uniformly, separates the 10 two compartments. The mixing process is performed in order to dissolve or uniformly suspend the BAS particles in the biodegradable vehicle. The resulting BAS-loaded biodegradable delivery systems can be free flowing or viscous liquids, gels or pastes In order to prepare a BAS-loaded delivery system just prior to use, the BAS and the biodegradable vehicle can be packaged in two separate containers as a kit. The vehicle and the BAS can 15 then be blended together by the aforementioned methods.
The biodegradable vehicles or BAS-loaded biodegradable delivery systems could be sterilized in the final package by an appropriate technique such as irradiation sterilization technique. Alternatively, the biodegradable vehicles or BAS-loaded biodegradable delivery systems can be prepared from pre-sterilized components in an aseptic 20 environment. Sterilization of the solvents and plasticizers used in the manufacturing process could be accomplished by an appropriate sterilization technique such as filtration, autoclaving or irradiation. The polymer and the BAS used to prepare the biodegradable vehicles and the BAS-loaded biodegradable delivery systems could also be sterilized by an appropriate sterilizing technique.
Advantages of the biodegradable vehicles described in the present invention include the ease of manufacturing, injection, implantation, and application, ease of control over the consistency or rheology and hydrophilicity or hydrophobicity of the biodegradable vehicle, flexibility of tailoring in vivo degradation kinetics of the vehicles, tailoring the dose of the BAS in the biodegradable delivery systems by blending the requisite amount of BAS 30 with the biodegradable vehicle, and enhancing stability of the BAS, especially when it is blended with the biodegradable vehicle just prior to its use. A major reason for the enhanced stability of the BAS is that the BAS is not subjected to exposure to solvents, chemicals or the harsh processing conditions especially during the manufacture of the biodegradable vehicle.
fr
Moreover, if the BAS is stored in an appropriate separate container, it does not come in contact with the biodegradable vehicle until it is blended with the vehicle.
Advantages of biodegradable delivery systems of the present invention include ease of manufacturing, injection, implantation, and application, ease of control over the 5 consistency or rheology and hydrophilicity or hydrophobicity of the biodegradable delivery systems, ease of incorporation of BAS into the delivery systems, facile tailoring of the release of BAS from the biodegradable delivery systems, and control of in vivo biodegradation rates of biodegradable delivery systems.
The biodegradable vehicles without blending any BAS may be used as a tissue 10 or cavity fillers or spacers in the body, whereas the biodegradable vehicles loaded with BAS may be used for the treatment of a variety of diseases and pathological conditions.
The final composition with or without the BAS may be injected, implanted, smeared or applied directly in animals, birds and humans.
Also described is a kit
comprising a) a biodegradable vehicle; and b) a BAS. In certain aspects, the BAS is blended with the biodegradable vehicle just prior to use. In certain aspects, the BAS is stored in a separate container in a solid state, liquid state (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers), or gel or paste (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers). Alternatively, a device, which 20 resembles two syringes or syringe-like devices (e.g. pumps in which materials can be mixed by depressing a trigger-like device) attached together with a removable partition or a valve assembly can also be used to uniformly mix the BAS with the biodegradable vehicle.
Further embodiments and advantages will become more apparent when read with the detailed descriptions and figures that follow.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 shows a method of preparing a biodegradable vehicle and delivery systems without the use of volatile solvents.
Figure 2 shows a method of preparing a biodegradable vehicle and delivery
systems.
Figure 3 shows an alternate method of preparing biodegradable delivery systems.
1
Figure 4 describes the effect of varying polymer to plasticizer ratios on cumulative amount of levonorgestrel released from biodegradable delivery systems.
Figure 5 describes the effect of varying polymer inherent viscosity on cumulative amount of levonorgestrel released from biodegradable delivery systems.
Figure 6 describes the effect of varying copolymer ratios on cumulative amount of levonorgestrel released from biodegradable delivery systems.
Figure 7 describes 1he effect of varying drug loadings on oxytetracycline base released from biodegradable delivery systems.
Figure 8 describes the effect of varying plasticizer compositions on 10 oxytetracycline base released from biodegradable delivery systems.
I Figure 9 describes the effect of varying plasticizer to polymer ratios on oxytetracycline base released from biodegradable delivery systems.
Figure 10 describes the effect of varying hydrophilicity of plasticizers on oxytetracycline base released from biodegradable delivery systems. 15 Figure 11 describes the effect of varying polymer to plasticizer ratios and plasticizer compositions on oxytetracycline base released from biodegradable delivery systems.
Figure 12 describes the effect of varying polymer molecular weights on oxytetracycline base released from biodegradable delivery systems. 20 Figure 13 describes the effect of varying drug solubility on naltrexone released from biodegradable delivery systems.
Figure 14 describes the effect of varying solubility of drug on oxytetracycline released from biodegradable delivery systems.
Figure 15 describes the effect of varying polymer molecular weights on 25 oxytetracycline base released from biodegradable delivery systems.
Figure 16 describes the effect of varying polymer molecular weights on in vivo release of oxytetracycline base from biodegradable delivery systems.
DETAILED DESCRIPTION OF THE INVENTION
In certain embodiments, the present invention relates to compositions of biodegradable vehicles and BAS-loaded delivery systems comprising at least one polymer and at least one plasticizer. The delivery system of the present invention may also comprise r
PCT/USO1/06138
of at least one biologically active substance (BAS). It also relates to the method of preparing biodegradable vehicles and delivery systems loaded with BAS.
According to the present invention, the term polymer includes oligomer, homopolymer, copolymer and terpolymer. Biodegradable polymers are used in this invention 5 because they form matrices that can control the release of BAS over a desired length of time, can degrade in vivo into non-toxic degradation products, and are available in varying physicochemical properties including varying hydrophilicity and hydrophobicity, varying molecular weights, varying crystallinity and amorphous states, and varying copolymer ratios.
In the present invention, plasticizers are used in varying ratios to convert a 10 polymer in a solid state to a biodegradable vehicle or delivery system of varying consistency such as a free flowing or a viscous liquid, a gel or a paste. Plasticizers are chemicals added to polymers to improve their flow, and therefore their processibility (Billmeyer, F., Jr. Textbook of Polymer Science, John Wiley and Sons, New York, 1984, p. 472). This is achieved by lowering their glass transition temperature (a temperature at which a glassy polymer becomes 15 rubbery on heating and a rubbery polymer reverts to a glassy one on cooling), thus achieving a change in properties. A plasticizer can only plasticize a polymer when the molecules of the plasticizer can interact with the molecules of the polymer. Hence, the plasticizers act like lubricants between the polymer chains, facilitating slippage of chain past chain under stress and extending the temperature range for segmental rotation to lower temperatures (Martin, 20 A., Physical Pharmacy, Lea and Febiger, Philadelphia, 1993, p. 588). The degree or extent of plasticization of a polymer will depend on the type and amount of plasticizer blended with the polymer. For example, higher the concentration of the plasticizer, greater the extent of plasticization or flexibility of the polymer. If a plasticizer and a polymer are fully compatible with each other, then depending on the concentration of the plasticizer blended with the 25 polymer, it is possible to obtain a polymer matrix of varying consistency or rheology such as a free-flowing or viscous liquid, gel or paste. Moreover, since plasticizers are available with varying physicochemical properties, including varying hydrophilicity and lipophilicity, it is possible to blend an appropriate plasticizer at a desired concentration with a selected compatible polymer such that the resulting biodegradable vehicle or BAS-loaded 30 biodegradable delivery system has the tailored physicochemical characteristics, including varying hydrophilicity and lipophilicity, and consistency. The present invention also includes formulations wherein two or more plasticizers are used in a combination or blend of varying ratios. The present invention also includes formulations wherein two or more polymers or
1
copolymers with varying copolymer ratios or molecular weights are used in a combination or blend of varying ratios.
Methods of preparing the biodegradable vehicles and delivery systems of the present invention involve dissolving at least one biodegradable polymer in a volatile solvent 5 or a mixture of solvents. At least one plasticizer is added to the resulting polymer solution. The volatile solvent is evaporated using vacuum or removed at an elevated temperature, or evaporated using a combination of both vacuum and elevated temperature. The resulting biodegradable vehicles and delivery systems could be in the form of either free-flowing or viscous liquids, gels or pastes. This method is particularly suited when polymers of high 10 molecular weights are used to prepare the vehicles or BAS delivery system, or when a high consistency of the biodegradable vehicle or BAS delivery system, is desired. Alternatively, one or more biodegradable polymers can be directly dissolved in one or more plasticizers by stirring the mixture with or without the use of heat. This method is particularly suited when polymers of low molecular weights are used to prepare the biodegradable vehicles or BAS 15 delivery system, or when a low consistency or BAS delivery system is desired.
Polymers suitable for preparing the biodegradable delivery systems of the present invention include, but are not limited to, homopolymers and/or copolymers of polyesters, polyorthoesters, polyphosphoesters, polyanhydrides, polyaminoacids, pseudopolyamino acids, polyamides, polyalkylcyanoacrylates, polyphosphazenes, 20 polydioxanone, poly(s-decaloactone), poly(glycolide-co-trimethylene carbonate), poly(ethylene carbonate), poly(iminocarbonate), poly(l,3-propylene malonate), poly(ethylene-l,4-phenylene-bis-oxyacetate), andpoly(ester-amides). In a preferred embodiment, polymers include polylactic acid or polylactide (PLA) and its copolymers, polyglycolic acid or polyglycolide and its copolymers, polycaprolactone (PCL) and its 25 copolymers, polyhydroxybutyrates and their copolymers, and polyhydroxyvalerates and polydioxanone and their copolymers. A mixture of polymers with different molecular weights or different types, or copolymer ratios may be used to tailor physicochemical properties, the degradation characteristics of the biodegradable vehicles and the delivery systems or the release characteristics of BAS from the biodegradable delivery systems, or 30 both.
Solvents used to dissolve the polymer for the preparation of biodegradable delivery system of the present invention include, but are not limited to, ketones, ethers,
alcohols, amides, and chlorinated solvents. Preferred solvents are acetone, ethyl acetate,
PCT/USO1/06138
methyl acetate, methylethylketone, chloroform, methylene chloride, isopropanol, ethyl alcohol, ethyl ether, methylethyl etiher, hexafluroisopropanol, tertrahydrofuran, and hexafluroacetone sesquihydrate. A mixture of volatile solvents may also be used to create a suitable mixture, which can dissolve both the polymer and the plasticizer.
Plasticizers used for the preparation of biodegradable delivery system of the present invention include, but are not limited to, citrates such as diethyl citrate (DEC),
triethyl citrate (TEC), acetyl triethyl citrate (ATEC), tributyl citrate (TBC), acetyl tributyl citrate (ATBC), butyryltri-n-hexyl-citrate, acetyltri-n-hexyl citrate, phthalates such as dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), dioctyl
phthalate, glycol ethers such as ethylene glycol diethyl ether, propylene glycol monomethyl ether, ethylene glycol monoethyl ether, diethylene glycol monoethyl ether (Transcutol®),
propylene glycol monotertiary butyl ether, dipropylene glycol monomethyl ether, N-methyl-
2-pyiroUdone, 2 pyrrolidone (2-Pyrrol®), isopropyl myristate, isopropyl palmitate,
dimethylacetamide, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate,
benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, sebacates such as dibutyl sebacate, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate,
propylene glycol laurate, propylene glycol caprylate/caprate, gamma butyrolactone,
polyethylene glycols (PEG), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree such as cotton seed oil, soy bean oil, almond oil, sunflower oil,
peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids (Gelucires®,
Labrafils® and Labrasol®) such as PEG-6 glycerol mono oleate, PEG-6 glycerol linoleate,
PEG-8 glycerol linoleate, PEG-4 glyceryl caprylate/caprate, PEG-8 glyceryl caprylate/caprate, polyglyceryl-3-oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate,
PEG-32 glyceryl laurate (Gelucire 44/1®), PEG-32 glyceryl palmitostearate (Gelucire
50/13®), PEG-32 glyceryl stearate (Gelucire 53/10®), glyceryl behenate, cetyl palmitate,
glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate (Triacetin®). The use of two or more plasticizers in a combination or blend of varying ratios is also encompassed by the present invention.
In order to prepare a BAS-loaded delivery system, the BAS can be loaded in
any physical form (i.e. solid, liquid, gel or paste, where the BAS is dissolved or suspended in the plasticizer or mixtures of plasticizers, volatile solvents or mixture of volatile solvents or mixtures of volatile solvents and plasticizers) at any step during the manufacturing process of biodegradable delivery systems before the volatile solvent is completely removed. It can also be manufactured by loading the BAS soon after the biodegradable vehicle is prepared, or
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blending the BAS to the biodegradable vehicle just prior to the use of the BAS-loaded biodegradable delivery system. Mixing the BAS with the biodegradable vehicle can be accomplished by simply stirring the mixture with a stirring device, or by triturating the mixture or employing an ointment mill or a suitable device or apparatus or equipment that 5 can be used for blending/mixing. When the BAS is blended with the biodegradable vehicle just prior to use, it could be stored in a separate container in a solid state, liquid state (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers), or gel or paste (where the BAS is dissolved or suspended in the plasticizer or blends of plasticizers). Alternatively, a device, which resembles two syringes or syringe-like devices (e.g. pumps in 10 which materials can be mixed by depressing a trigger-like device) attached together with a removable partition or a valve assembly can also be used to uniformly mix the BAS with the biodegradable vehicle. The BAS is loaded in one syringe or compartment and the biodegradable vehicle is loaded in the other compartment. A removable partition or a valve, which will allow the contents of the two compartments to be mixed uniformly, separates the 15 two compartments. The mixing process is performed in order to dissolve or uniformly suspend the BAS particles in the biodegradable vehicle. The resulting BAS-loaded biodegradable delivery systems can be free flowing or viscous liquids, gels or pastes. In order to prepare a BAS-loaded delivery system just prior to use, the BAS and the biodegradable vehicle can be packaged in two separate containers as a kit. The vehicle and 20 the BAS can then be blended together by the aforementioned methods.
The procedure for preparing a biodegradable vehicle first, loading the BAS soon after the biodegradable vehicle is prepared, or blending the BAS to the biodegradable vehicle just prior to the use of the BAS-loaded biodegradable delivery system is shown in Figures 1 and 2.
The procedure of loading BAS before removing the volatile solvent or mixture of volatile solvents to prepare biodegradable delivery systems is shown in Figure 2.
However, the method of addition of the BAS is not limited to that shown in Figure 2, since the BAS can be loaded in any physical form (i.e. solid, liquid, gel or paste, where the BAS is dissolved or suspended in the plasticizer or mixtures of plasticizers, volatile solvents or 30 mixture of volatile solvents or mixtures of volatile solvents and plasticizers, at any step during the manufacturing process, before the volatile solvent is completely removed.
The resulting BAS-loaded biodegradable delivery systems can be free flowing or viscous liquids, gels or pastes, wherein the BAS can be dissolved or suspended.
Examples of BAS include, but are not limited to, steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS - agents), narcotic agonists and antagonists, fertility regulating agents, antibodies and antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and 5 infection preventing agents, cardiovascular agents, angiogenic and antiangiogenic agents, anti-inflammatory agents, immunomodulators, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, live or killed bacteria and viruses, agents or extracts derived from whole or parts of plants, trees, flowers, fruits, buds, seeds, leaves, barks, stem, roots, and animal tissues, growth promoting agents, soft and hard tissues, growth factors, human 10 growth factor, human growth hormone, FGF, erythropoietin, Nupagen, granulocyte colony-stimulating factor (G-CSF), cells, tissues such as bones or agents derived there from, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, genes, nucleic acid, antisense, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid (RNA), RNA fragments, and biological tissues 15 such as islets of langerhans and pancreas, insulin, vitamin and mineral supplements, iron, chelating agents, coagulants, anticoagulants, and the like.
In certain aspects, the bioactive agents include anticancer agents such as taxol, carmustine, interleukin 2, interferon, growth hormones such as human growth hormone, somatotropin hormone, antipsychotic agents such as risperidone, antibiotics such as 20 gentamicin, tetracycline, oxytetracycline, topical anesthetic agents such as benzocaine, chloroprocaine, cocaine, procaine, propoxycaine tetracaine, depravaine, bupivacaine, etidocaine, levobupivacaine, lidocaine, mepivacaine, prilocaine, propofol and ropivacaine, analgesic agents such as morphine, oxycodone, fentanyl, fentanyl, sufentanyl, butorphanol, narcotic antagonists such as naltrexone, nalorphine, naloxone, nalmefene, growth promotic 25 agents such as TGF alpha and TGF beta, bone morphogenic peptides and proteins and calcium salts such as calcium sulfate, calcium phosphate, and anti-inflammatory agents such as dichlofenac. In one preferred aspect, the present invention provides a biodegradable vehicle comprising oxytetracycline for veterinary use.
In certain other embodiments, the biologically active agents include, but are 30 not limited to, steroids such as protaglandins, estrogens, androgens, and progestins;
ophthalmics such as lubricants and anti-glaucoma; antibiotics such as quinolones; saliva subsitiutes, sedative/hypnotics such as benzodiazepines and barbituates; wound care such as growth factors (EPO, FGF, G-CSF); antiparasitics (worms, malarial); anticonvulsants, muscle relaxants, nucleoside analogs, osteoporosis preparations (supplement bone growth),
antiparkinsonian agents, antibiotics such as cephalosporins, aminoglycosides and sulfonamides, oxytocic agents and prostaglandins.
Those of skill in the art will know of other biological agents useful in the practice of the present invention.
The physical form (i.e. liquids, gels or pastes), consistency or rheology,
hydrophilicity or hydrophobicity, in vivo duration of stay of the biodegradable vehicles or delivery systems, in vivo biodegradation rate of biodegradable vehicles or delivery systems, and BAS release characteristics from BAS-loaded biodegradable delivery systems depend on a number of factors. These include: type of polymer or copolymer, hydrophilicity or 10 lipophilicity of polymer or copolymer, concentration of polymer or copolymer, molecular weight of polymer or copolymer, copolymer ratios, combination of polymers or copolymers with different molecular weights, combination of copolymer with varying copolymer ratios, combination of different types of polymer with varying crystallinity, hydrophilicity or hydrophobicity, type of plasticizer, hydrophilicity or lipophilicity of plasticizer, concentration 15 of plasticizer (polymer or copolymer to plasticizer/plasticizers ratios), combination of plasticizers, type of BAS, loading of BAS, hydrophilicity or lipophilicity of BAS, molecular weight of BAS. In addition, the physicochemical interactions between the polymer, plasticizer and BAS also affect the above-mentioned properties of biodegradable vehicles and delivery systems.
For example, using the present invention, it is possible to tailor the release of a
BAS (with specific physicochemical properties and the desired in vivo concentration), for the desired length time. This is achieved by blending an appropriately selected polymer or polymers with an appropriately selected plasticizer or mixtures of plasticizers. Besides controlling the release characteristics of the BAS from the delivery system described in the 25 present invention, a blend of the appropriate polymer or polymers and plasticizer also controls the consistency or rheology of the delivery system.
It is also possible to extend the in vivo duration of stay of the biodegradable vehicle or delivery system by selecting a higher molecular weight or highly hydrophobic polymer, since polymers with higher molecular weights or high hrdrophobicity generally 30 degrade slowly in the body. Furthermore, it is possible to modify the degradation kinetics of the biodegradable vehicle or delivery system, or obtain pulsatile or intermittently fluctuating delivery of the BAS from the BAS-loaded delivery systems by combining polymers of different molecular weights (e.g. low, intermediate and high molecular weights or low and high molecular weights or low and medium molecular weights or medium and high n
molecular weights), whereby the low molecular weight polymer in the biodegradable vehicle may degrade at a much faster rate than the rest of the polymer in the blend. Alternatively, using blends of copolymers of different copolymer ratios of varying hydrophilicity and hydrophobicity (e.g. different copolymer ratio of lactide-glycolide or lactide-caprolactone) or 5 using blends of two different polymers or copolymers with different ciystallinity (e.g. blends of polyacaprolactone and polylactic acid or polycaprolactone and poly-lactic-co-glycolic acid/polylactide-co-glycolide (PLGA)) can also result in a biodegradable vehicle or biodegradable delivery system with varying degradation kinetics where the more hydrophilic or amorphous polymer may degrade at a much faster rate than the rest of the polymers in the 10 blend.
The biodegradable vehicle without any BAS may be used as a biodegradable tissue or cavity filler or spacer in the body, whereas, BAS-loaded biodegradable delivery system may be used for the treatment of a variety of diseases and pathological conditions. The final composition with or without the BAS may be injected, implanted, smeared or 15 applied in animals, birds or humans.
For example, the biodegradable delivery system loaded with an antitumor agent or antiangiogenic agent can be directly injected into or adjacent to solid tumors such as brain tumor, breast tumors, melanomas, etc. It can also be injected, implanted or smeared at a site from where a solid tumor has been surgically removed, thus affording site-specific 20 delivery for disease states that are otherwise very difficult, (if not impossible) to treat using the conventional methods of treatment. For localized BAS delivery and treatment, BAS-loaded biodegradable vehicle can also be used in surgeries where appropriate quantities of an antibiotic, an anti-inflammatory agent, a local anesthetic or analgesic, or combinations thereof can be loaded in the biodegradable vehicle by the surgeon in an operating room, and 25 the resulting mixture can then be injected, implanted, smeared or applied at the site of surgery to minimize the chances of localized infections or inflammation and reduce pain respectively, due to surgery. In the case of orthopedic surgery, currently, the majority of the orthopedic surgeons prepare beads in the operating room with a non-biodegradable polymer, polymethylmethacrylate (PMMA). These beads are loaded with an appropriate dose of an 30 antibiotic. These beads are then placed in the cavity at the site of surgery to prevent infections such as osteomyelitis. However, the non-degradable polymer beads have to be eventually removed before closing the wound witibt a suture, and the patients are then given an intravenous dose of an antibiotic or treated with an oral antibiotic. This procedure can easily be corrected with the use of an antibiotic loaded biodegradable vehicle that can be injected,
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implanted, smeared or applied near or at the site of surgeiy. High concentrations of the antibiotic at the site of surgery can prevent infections. Moreover, the BAS delivery system need not be removed from the site of administration because of the biodegradable nature of the system. The biodegradable vehicle loaded with bone growth promoting agents such as 5 calcium sulfate, calcium phosphate or hydroxyapatite can be injected, implanted, applied or smeared at an appropriate site, where it is needed following bone, disc or spine surgeiy. BAS such as low molecular weight heparin can also be incorporated into the biodegradable vehicle and the resulting mixture can be used to treat conditions such as deep venous thrombosis (DVT) in trauma or surgical patients.
The system could be loaded with a contraceptive agent, antipsychotic agent,
anticonvulsants, antimalarial, antihypertensive agent, antibiotics, antiviral agents, biologically active protein and peptides, vaccines, live or killed bacteria and viruses, genes, DNA or DNA fragments, RNA or RNA fragments, and injected, implanted, smeared or applied in the body to provide a controlled release of the agents for the desired length of time. Biodegradable 15 delivery system loaded with BAS such as antiinflammatory agents, analgesics and anesthetics could be injected directly into joints or sites in the body from where the pain is emanating, thus providing relief from the excruciating pain and making the joints more mobile. Antigens may also be incorporated into the delivery system and injected, implanted or applied in animals or humans to induce the production of specific antibodies. Bones (fragments or 20 powder), morphogenic proteins such as growth promoting agents of biological tissues and organs and wound-healing factors, can also be incorporated into the biodegradable vehicle, and the resulting mixture is injected, implanted or applied at the site of administration. Live cells and/or whole or a part of a tissue or tissues and organs can also be blended with the biodegradable vehicle and injected, implanted or applied at the site of administration. For 25 pulsatile or intermittent delivery of BAS such as vaccines, the biodegradable vehicle can be prepared with blends of varying molecular weights of polymers or copolymers, or with blends of copolymers of varying copolymer ratios (e.g. 50/50 PLGA and 85/15 PLGA or 100% PLA and 25/75 PLGA) or blends of different types of biodegradable polymers with varying hydrophobicity or lipophilicity or crystallimty (e.g. 1:1 ofPLA:PCL or 1:3 of 30 PLA:PCL or 1:1 of 50/50 PLGA:PCL).
The formulation, which is sterile, is suitable for various topical or parenteral routes, such as intramuscular, subcutaneous, intra-articular, by suppository (e.g. per-rectum or vaginal application), intradermal. In certain aspects, the biological active agents and biodegradable delivery systems are delivered or administered topically. Additionally, the
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agents can be delivered parenterally. Topical administration is preferred in treatment of lesions of the skin as in psoriasis, where such direct application is practical and clinically indicated.
An effective quantity of the compound of interest is employed in treatment.
The dosage of compounds used in accordance with the invention varies depending on the compound and the condition being treated. For example, the age, weight, and clinical condition of the recipient patient; and the experience and judgment of the clinician or practitioner administering the therapy are among the factors affecting the selected dosage. Other factors include: the route of administration, the patient, the patient's medical history,
the severity of the disease process, and the potency of the particular compound. The dose should be sufficient to ameUorate symptoms or signs of the disease treated without producing unacceptable toxicity to the patient. In general, an effective amount of the compound is that which provides either subjective relief of symptoms or an objectively identifiable improvement as noted by the clinician or other qualified observer.
This invention will be understood with greater particularity by reviewing the following examples:
EXAMPLES
EXAMPLE 1
Preparation of a biodegradable vehicle:
A polymer (50% w/w of 50/50 lactide-co-glycolide copolymer) was dissolved in minimum quantity of acetone. Triethyl citrate (TEC), at a concentration of 50% w/w, was added to the polymer solution and was stirred to yield a uniform mixture. Acetone was evaporated from the mixture by heating at 60-75°C with constant stirring. The resulting
formulation obtained was a matrix with a gel-like consistency.
EXAMPLE 2
Example 1 was repeated using 10% w/w of 50/50 lactide-co-glycolide copolymer and 90% w/w TEC. The resulting formulation obtained was a matrix with a liquid-like consistency.
EXAMPLE 3
Example 1 was repeated using 20% w/w of 50/50 lactide-co-glycolide copolymer and 80% w/w TEC. The resulting formulation obtained was a matrix with a viscous liquid-like consistency.
EXAMPLE4
Example 1 was repeated, using 30% w/w of 50/50 lactide-co-glycolide copolymer and 70% w/w TEC was used. The resulting formulation obtained was a matrix with a viscous liquid-like consistency.
EXAMPLE 5
Example 1 was repeated, using 40% w/w of 50/50 lactide-co-glycolide copolymer and 60% w/w TEC was used. The resulting formulation obtained was a matrix with a viscous liquid-like consistency.
EXAMPLE 6
Example 1 was repeated, using 60% w/w of 50/50 lactide-co-glycolide 10 copolymer and 40% w/w TEC was used. The resulting formulation obtained was a matrix with a gel-like consistency.
EXAMPLE 7
Example 1 was repeated, using 70% w/w of 50/50 lactide-co-glycolide copolymer and 30% w/w TEC was used. The resulting formulation obtained was a matrix 15 with a gel-like consistency.
EXAMPLE 8
Example 1 was repeated, using 80% w/w of 50/50 lactide-co-glycolide copolymer and 20% w/w TEC was used. The resulting formulation obtained was a matrix with thick sticky paste.
EXAMPLE 9
Example 1 was repeated with the following polymers and plasticizers as shown in Table 1 below:
ir
TABLE 1
TYPE OF POLYMER
PLASTICIZER
SOLVENT
DESCRIPTION OF THE FORMULATION
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
GLYCERYL TRIACETATE (TRIACETIN)
ACETONE
GEL, SLIGHTLY CLOUDY
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
TRIETHYL CITRATE (TEC)
ACETONE
GEL, TRANSPARENT
DL-POLYLACTIC ACID ("DL-PLA; I.V. = 0.58)
ACETYL TRIETHYL CITRATE (ATEC)
ACETONE
GEL, SLIGHTLY CLOUDY
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
DIMETHYL PHTHALATE (DMP)
ACETONE
GEL, LESS VISCOUS, TRANSPARENT
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
DIETHYL PHTHALATE (DEP)
ACETONE
GEL, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
GLYCERYL TRIACETATE (TRIACETIN)
ACETONE
GEL, LESS VISCOUS, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLY COLIC ACID (DL-PLGA; I.V. = 0.58)
TRIETHYL CITRATE (TEC)
ACETONE
GEL, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
ACETYL TRIETHYL CITRATE (ATEC)
ACETONE
GEL, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLY COLIC ACID (DL-PLGA; I.V. = 0.58)
TRIETHYL CITRATE (TEC)
ACETONE
GEL, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
DIMETHYL PHTHALATE (DMP)
ACETONE
GEL, LESS VISCOUS, RANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
DIETHYL PHTHALATE (DEP)
ACETONE
GEL, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
N-METHYL PYRROLIDONE (NMP)
ACETONE
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
GLYCERYL TRIACETATE (TRIACETIN)
ACETONE
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
TRIETHYL CITRATE (TEC)
ACETONE
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
ACETYL TRIETHYL CITRATE (ATEC)
ACETONE
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
TRIETHYL CITRATE (TEC)
ACETONE
VISCOUS LIQUID, TRANSPARENT
EXAMPLE 10
Several polymers were separately dissolved in several volatile solvents.
Several plasticizers were separately added to the polymer-solutions, such that the ratio of polymer to plasticizer in the final formulations ranged from 1:19 to 4:1. Several drugs were separately added to the polymer-plasticizer-solvent blends. The solvents were then 10 evaporated at an elevated temperature to obtain drug-loaded formulations. The drug content in the final formulations constituted up to 50% w/w.
For several formulations, blank formulations of polymers and plasticizers blends were JSrst obtained. The drugs were then separately added to the blank formulations to obtain drug-loaded formulations. Table 2 lists examples of polymers, plasticizers, solvents, 15 polymer to plasticizer ratio and concentration of drugs in the formulations.
(1
TABLE 2
TYPE OF POLYMERS
PLASTICIZERS
SOLVENTS
POLYMER TO
PLASTICIZER RATIOS
DRUGS
CONCENTRATION OFDRUGS (% w/w) IN
POLYMER MATRICE S
POLYCAPROLAC-
DIETHYLENE
METHYLENE
1:1
TESTOSTERONE
TONE
GLYCOL
CHLORIDE
MONOETHYL ETHER
1:2
PROGESTERONE
0.5% -50%
POLYLACTIC ACID
(TRANSCUTOL®),
CHLOROFORM
w/w
1:3
LEVONORGESTREL
POLYLACTIC-CO-
PEG-8-GLYCERYL
ACETONE
GLYCOLIC ACID
CAPRYLATE/CAPRA
1:4
THEOPHYLLINE
TE
ETHYL ACETATE
COPOLYMERS OF
(LABRASOL®)
1:9
PROPRANOLOL
LACTIC ACID AND
CAPROLACTONE
TRIETHYL CITRATE
1:19
ATENOLOL
(TEC),'
2:1
METOPROLOL
ACETYL TRIETHYL
CHLORPROAMAZINE
CITRATE (ATEC)
2:3
CLONIDINE
GLYCERYL
3:2
TRIACETATE
INSULIN
(TRIACETIN®)
3:1
OXYTETRACYCLINE
POLYETHYLENE
4:1
GLYCOLS (PEG)
NALTREXONE
N-METHYL
PYRROLIDONE
(NMP)
EXAMPLE 11
Effect of varying polvmer-to-plasticizer ratios on the physical state of 5 formulations and drug release characteristics
Several samples of polylactic-co-glycolic acid (inherent viscosoty - 0.59) were weighed and separately dissolved in acetone. Varying ratios of N-methyl pyrrolidone (NMP) were separately added to the polymer-solutions, such that the ratio of polymer to plasticizer in the formulations ranged from 20:80 to 80:20. Acetone was then evaporated by heating the 10 solutions at 70-80°C. Levonorgestrel (2% w/w) was added to the resulting formulations. Table 3 describes the physical state of the formulations containing varying polymer-to-plasticizer ratios. Drug release characteristics from the formulations depicted in Table 3 are shown in Figure 3.
>Q
WO 02/00137 PCT/USO1/06138
TABLE 3
Physical state of formulations prepared with varying polymer-to-plasticizer ratios
Polymer*-to-NMP Ratio
Physical State of the Formulation
Physical State of Drug in the Formulation
: 80
Very flowable liquid
Dissolved
40:60
Viscous liquid
Dissolved initially; however precipitated partially after 48 hrs
50:50
Flowable gel
Suspended
60:40
Flowable gel
Suspended o
<N
O OO
Thick paste
Suspended
* 50/50 Polylactide-co-glycolide (IV=0.59 dL/g)
Drug loading = 2% w/w
EXAMPLE 12
Effect of varying oolvmer inherent viscosities on the physical state of the formulations and drug release characteristics
Several samples of polylactic-co-glycolic acid (PLGA) with varying inherent viscosities ranging from 0.15 -1.07) were weighed and separately dissolved in acetone. An appropriate quantity of N-methyl pyrrolidone (NMP) was added to the polymer-solutions 15 such that the ratio of polymer to plasticizer in the formulations was 33% PLGA and 67% NMP. Acetone was then evaporated by heating the solutions at 70-80°C. Levonorgestrel (2% w/w) was added to the resulting formulations. Table 4 describes the physical state of the formulations containing varying polymer inherent viscosities. Drug release characteristics from the formulations depicted in Table 4 are shown in Figure 4.
TABLE 4
Physical state of formulations prepared with polymer of varying inherent viscosities
Polymer Inherent Viscosity (dL/g)
Physical State of the Formulation*
Physical State of Drug in the Formulation*
0.15
Very flowable liquid
Dissolved
0.26
Flowable liquid
Dissolved
0.42
Flowable liquid
Dissolved
0.59
Viscous liquid
Dissolved
0.74
Flowable gel
Dissolved
1.07
Viscous gel
Dissolved
*33% w/w of 50/50 Polylactide-co-glycolide and 67% w/w NMP
Drug loading = 2% w/w
EXAMPLE 13
Effect of varying copolymer ratios on physical state of formulations and drug 10 release characteristics
Samples of pure polylactic acid and polylactic-co-glycolic acid (PLGA) with varying copolymer ratios ranging from 50/50 to 85/15 were weighed and separately dissolved in acetone. An appropriate quantity of N-methyl pyrrolidone (NMP) was added to the polymer-solutions such that the ratio of polymer to plasticizer in the formulations was 33% 15 PLGA and 67% NMP. Acetone was then evaporated by heating the solutions at 70-80°C. Levonorgestrel (2% w/w) was added to the resulting formulations. Table 5 describes the physical state of the formulations prepared from varying copolymer ratios. Drug release characteristics from the formulations depicted in Table 5 are shown in Figure 5.
PCT/USO1/06138
TABLE 5
Physical state of formulations prepared with polymers of varying copolymer ratios.
Ratio of Lactide to Glycolide in Polymer
Physical State of the Formulation*
Physical State of Drug in the Formulation*
50/50
Yellowish, viscous liquid
Dissolved
65/35
Yellowish, viscous liquid
Dissolved
75/25
Pale yellow, highly viscous liquid
Dissolved
85/15
Straw colored, slightly translucent, highly viscous liquid
Dissolved
100/0
Clear, highly viscous liquid
Dissolved
* 33% w/w of Polylactide-co-glycolide and 67% w/w NMP Drug loading = 2% w/w
EXAMPLE 14
Effect of varying drug loadings on drug release
A polymer (25% w/w of 50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.59) was dissolved in a minimum quantity of acetone. Pure polyethylene glycol 400 (PEG 400) was added to the polymer solution. The solution was stirred to yield a uniform mixture. Acetone was evaporated from the mixture by heating at 60-75°C with 15 constant stirring. The blank formulation was kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulation obtained was a matrix with a viscous liquid like consistency. Three different concentrations of oxytetracycline base (either 10,20 or 30% w/w) were added to the blank formulation and mixed thoroughly to ensure uniform distribution of the drug in the formulations. Drug release from the drug-loaded 20 formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 6 shows the cumulative amount of oxytetracycline released from formulations prepared with the above-mentioned compositions. Increasing the percentage of drug in the formulations from 10 to 30% w/w increased the cumulative amount of drug released at the end of 360 hours. This increase occurred because, at higher drug-loadings, 25 more drug is available on the surface of the formulations for release. Moreover, a higher
PCT/USO1/06138
drug concentration gradient between the formulation and the dissolution medium is expected at 30% w/w drug-loading compared to the one at 10% w/w drug loading.
EXAMPLE 15
Effect of plasticizer compositions on drug release 5 A polymer (25% w/w of 50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.59) was dissolved in a minimum quantity of acetone. Either pure triethyl citrate (TEC), or polyethylene glycol 400 (PEG 400), or blends of PEG 400 and TEC (either 50/50% or 75/25% blends of PEG 400/TEC) was added to the polymer solution. The resulting solutions were stirred to yield uniform mixtures. Acetone was evaporated from the 10 mixtures by heating at 60-75°C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulations obtained were matrices with a viscous liquid like consistency. Oxytetracycline base (20% w/w) was added to each blank formulation and mixed thoroughly to ensure uniform distribution of the drug in the formulations. Drug release from the drug-loaded 15 formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 7 shows the cumulative amount of oxytetracycline released from formulations prepared with the above-mentioned compositions. Increasing the percentage of PEG 400 in the formulations prepared from 0% PEG 400 and 100% TEC to 100% PEG 400 and 0% TEC resulted in faster drug release. This is because PEG 400 is very hydrophilic and 20 is completely miscible in water, whereas, the aqueous solubility of TEC is approximately 6%.
EXAMPLE 16
Effect of varying ratios of polvmer and plasticizer on drug release
Three different concentrations (10, 20 or 25% w/w) of a polymer (50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.59) were dissolved in a minimum 25 quantity of acetone. Pure PEG 400 (90, 80 or 75% w/w) was added to the polymer solutions. The solutions were stirred to yield uniform mixtures. Acetone was evaporated from the mixtures by heating at 60-75°C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulations obtained were matrices with varying viscosities or consistency. The 30 formulation with 25% polymer was considerably more viscous than the one with 10%
polymer. Oxytetracycline base (20% w/w) was added to each blank formulation and mixed thoroughly to ensure uniform distribution of the drug in the formulations. Drug release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 8 shows the cumulative amount of oxytetracycline
released from formulations prepared with the above-mentioned compositions. It is evident from the figure that decreasing the percentage of polymer in the formulations from 25% to 10% dramatically increased the drug release. This is because a decrease in polymer concentration from 25% to 10% and a corresponding increase in the plasticizer concentration 5 from 75% to 90% resulted in a decrease in the glass transition temperature, viscosity and an increase in polymer chain mobility of the formulations. Hence, the formulation with 10% polymer offered considerably less resistance for drug diffusion through the matrix compared to the one prepared with 25% polymer.
EXAMPLE 17
Effect of varying plasticizer hvdrophilicitv on drug release
A polymer (25% w/w of 50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.59) was dissolved in a minimum quantity of acetone. Either pure polyethylene glycol 400, triethyl citrate (TEC) or acetyl triethyl citrate (ATEC) was added to the polymer solution. The resulting solutions were stirred to yield uniform mixtures. Acetone was 15 evaporated from the mixtures by heating at 60-75 °C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulations obtained were matrices with a viscous liquid like consistency. Oxytetracycline base (20% w/w) was added to each blank formulation and mixed thoroughly to ensure uniform distribution of the drug in the formulations. Drug 20 release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 9 shows the cumulative amount of oxytetracycline released from formulations prepared with the above-mentioned compositions. It is evident from the figure that drug release was fastest from formulations prepared with PEG 400, and slowest from those prepared with ATEC. Intermediate drug release was 25 observed from formulations prepared from TEC. This is because PEG 400 is completely miscible with water, whereas, the solubility of TEC in water is approximately 6% and ATEC is almost insoluble in water with an aqueous solubility of less than 0.1%.
EXAMPLE 18
Effect of varying polvmer to plasticizer ratios and plasticizer compositions on 30 drug release
Blank formulations were prepared by dissolving either 16.67% w/w or 25% w/w of 50/50 polylactide-co-glycolide copolymer (inherent viscosity of 0.59) and either 50/50% or 75/25% blends of PEG 400 and TEC in a minimum quantity of acetone. The resulting solutions were stirred to yield uniform mixtures. Acetone was evaporated from the
mixtures by heating at 60-75°C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulations obtained were matrices with a viscous liquid like consistency. Oxytetracycline base (20% w/w) was added to each blank formulation and mixed thoroughly to ensure
uniform distribution of the drug in the formulations. Drug release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 10 shows the cumulative amount of oxytetracycline released from formulations prepared with the above-mentioned compositions. It is evident from the figure that faster drug release was observed from formulations prepared with a 16.67% polymer and
83.3% of plasticizer blends of varying compositions (polymer to plasticizer ratio of 1:5)
compared to those prepared from formulations with polymer to plasticizer ratios of 1:3 (25%
polymer and 75% plasticizer). This is because increasing the polymer concentration in the formulations from 16.67% to 25% increased the viscosity of the formulations and decreased the drug diffusion from the formulations. Moreover, a comparison of drug released from
formulations with similar polymer to plasticizer ratios but varying plasticizer compositions revealed that drug release was considerably faster from formulations prepared with blends of
75% PEG 400 and 25% TEC compared to those prepared from 50/50% blend of PEG
400/TEC. This is because the PEG 400 is completely miscible in water, whereas, the aqueous solubility of TEC in water is approximately 6%.
EXAMPLE 19
Effect of varying polvmer inherent viscosities on drug release
Four different inherent viscosities (i.v. = 0.15, 0.26, 0.59 and 0.76) of a polymer (50/50 lactide-co-glycolide copolymer) were dissolved in a minimum quantity of acetone. Pure PEG 400 was added to the polymer solutions. The solutions were stirred to
yield uniform mixtures. Acetone was evaporated from the mixtures by heating at 60-75°C
with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C
overnight to ensure complete removal of acetone. The resulting formulations obtained were matrices with varying viscosities or consistency. The formulation prepared with the polymer of inherent viscosity of 0.76 was considerably more viscous than the one prepared with the
3 0 polymer of inherent viscosity of 0.15. Oxytetracycline base (20% w/w) was added to each blank formulation and mixed thoroughly to ensure uniform distribution of the drug in the formulations. Drug release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 11 shows the cumulative amount of oxytetracycline released from formulations prepared with the above-
H
PCT/USO1/06138
mentioned compositions. It is evident from the figure that decreasing the inherent viscosity of polymer from 0.76 to 0.15 dramatically increased the drug release. This is because a decrease in polymer inherent viscosity resulted in a dramatic decrease in the viscosity of the formulation and a corresponding decease in resistance to drug diffusion from the matrix.
EXAMPLE 20
Effect of varying drug solubility on drug release
Blank formulations were prepared by dissolving 25% of a polymer (50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.64) and pure PEG 400 or 50/50% blends of PEG 400 and TEC in a minimum quantity of acetone. The solutions were stirred to 10 yield a uniform mixture. Acetone was evaporated from the mixtures by heating at 60-75°C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The resulting formulations obtained were a matrix with viscous liquid-like consistency. Either hydrated naltrexone base (20% w/w) or naltrexone hydrochloride (20% w/w) was added to the blank formulations and mixed 15 thoroughly to ensure uniform distribution of the drugs in the formulations. Drug release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer. Figure 12 shows the cumulative amount of either hydrated naltrexone base or naltrexone hydrochloride released from formulations prepared with the above-mentioned compositions. The release of naltrexone hydrochloride is considerably faster from formulations prepared with both pure 20 PEG 400 and 50/50% blends of PEG 400 and TEC than the release of the hydrated naltrexone base from similar formulations. This is because the solubility of the naltrexone hydrochloride in the dissolution buffer is much greater than that of the hydrated naltrexone base.
A similar drug release study was performed with formulations containing either 20% oxytetracycline hydrochloride or 20% oxytetracycline base. The blank
formulations were prepared by dissolving 25% of a polymer (50/50 lactide-co-glycolide copolymer, inherent viscosity of 0.59) and 75% of pure PEG 400 in a minimum quantity of acetone. The solutions were stirred to yield a uniform mixture. Acetone was evaporated from the mixtures by heating at 60-75°C with constant stirring. The blank formulations were kept in a vacuum oven at 60-75°C overnight to ensure complete removal of acetone. The
resulting formulations obtained were a matrix with viscous liquid-like consistency. Either
% oxytetracycline hydrochloride or 20% oxytetracycline base was added to the resulting formulations and mixed thoroughly to ensure uniform drug distribution. Drug release from the drug-loaded formulations was performed at 37°C in isotonic phosphate buffer containing sodium sulfite as an antioxidant. Figure 13 shows the cumulative amount of oxytetracycline
3-7: 1
PCT/USO1/06138
released from formulations prepared with the above-mentioned compositions. It is evident from the figure that the release of oxytetracycline hydrochloride is considerably faster than the release of oxytetracycline base from similar formulations. This is because of the greater aqueous solubility of the hydrochloride salt than the base.
EXAMPLE 21
Biodegradable delivery systems could be prepared by the procedures shown in Examples 1-20. Instead of adding a single biologically active agent, a combination of two or more biologically active agents could be incorporated together in the said delivery system. Examples of some of the combinations of the biologically active agents include 10 levonorgestrel and ethinyl estradiol, trimethoprim and sulfamethoxazole, trimetrexate and leucovorin, isoniazid, rifampin and ethambutol, dapsone and rifampicin, erythromycin and rifampicin, clotrimazole and nystatin, amphotericin B and flucytosine, hydrochlorothiazide and amiloride, hydrochlorothiazide and spironolactone, hydrochlorothiazide and captopril, polythiazide and reserpine. Moreover, instead of adding a single plasticizer, a combination of 15 two or more plasticizers could be added to obtain a formulation with the desired consistency and hydrophilicity or hydrophobicity. An example of a combination of plasticizer is acetyl triacetyl citrate (ATEC), n-methyl pyrrolidone (NMP) and a vegetable oil such as sesame oil, olive oil, safflower oil, sunflower oil, cottonseed oil or almond oil.
EXAMPLE 22
Biodegradable vehicle could be prepared by the procedures shown in
Examples 1-20. The vehicle could be loaded with BAS in a pharmacy or in an operating room by the health practitioner (a pharmacist, surgeon, nurse), just prior to administration to the patient, with an appropriate quantity of an antitumor agent and injected directly into a solid tumor or at a site from where a solid tumor has been surgically removed. Alternatively, 25 biodegradable vehicle loaded with an antitumor agent can also be injected into the tumor, or injected, implanted, smeared or applied at the site from where the tumor is removed by the surgeon.
EXAMPLE 23
A similar treatment described in Example 22 can be offered to patients with 30 brain tumors where the biodegradable vehicle prepared by the methods shown in Examples 1-20 and loaded with an appropriate quantity of an antitumor agent. The BAS-loaded delivery system can be injected, implanted or applied directly at the site in the brain from where the tumor has been removed.
EXAMPLE 24
The biodegradable vehicle prepared as shown in examples 1-20 and loaded with a BAS such as an antibiotic an anti-inflammatory agent, a local anesthetic or analgesic, or combinations thereof can also be used in surgeries where appropriate quantities of the 5 BAS, can be mixed with the biodegradable vehicle by the surgeon in an operating room, and the resulting mixture can then be injected, implanted, smeared or applied at the site of surgery to minimize the chances of localized infections or inflammation and reduce pain respectively, due to surgery. Alternatively, an antibiotic loaded biodegradable vehicle can also be injected, implanted, smeared or applied at the site of surgery by the surgeon at the site of surgery. 10 EXAMPLE 25
In the case of orthopedic surgery, a biodegradable vehicle prepared by the method shown in examples 1-20 and loaded with an antibiotic can be injected, implanted, applied or smeared near or at the site of surgery. High concentrations of the antibiotic at the site of surgeiy can prevent infections. Moreover, the B AS delivery system need not be 15 removed from the site of administration because of the biodegradable nature of the system.
EXAMPLE 26
The biodegradable vehicle prepared with the methods described in examples 1-20 and loaded with bone (fragments or powdered) or bone growth promoting agents such as calcium sulfate, calcium phosphates or hydroxyapatite can be injected, implanted, applied or 20 smeared at an appropriate site where it is needed following orthopedic surgery.
EXAMPLE 27
The biodegradable vehicle prepared with the methods described hi examples 1-20 and loaded with a low molecular weight heparin can also be used to treat conditions such as deep venous thrombosis (DVT) in trauma or surgical patients. 25 EXAMPLE 28
For pulsatile or intermittent delivery of BAS such as vaccines, live or killed viruses or bacteria, the biodegradable vehicle prepared with the methods described in examples 1-20 can be prepared with blends of varying molecular weights of polymers or copolymers, or with blends of copolymers of varying copolymer ratios such as 50/50 PLGA 30 and 85/15 PLGA or 100% polylactic acid (PLA) and 25/75 PLGA, or blends of different types of biodegradable polymers with varying hydrophobicity or lipophilicity or crystallinity such as 1:1 ofPLA:PCL or 1:3 of PLA:PCL or 1:1 of 50/50 PLGA:PCL.
EXAMPLE 29
PCT/USO1/06138
The polymer (50/50 lactide-co-glycolide copolymer) was dissolved directly in various plasticizers with stirring with or without the use of heat. Specific examples of formulations prepared using this method are listed in Table 6 below. The resulting formulations obtained were a matrix with a viscous liquid or gel-like consistency.
Table 6: Description of formulations prepared by directly mixing the polymer with the plasticizer with or without the use heat
TYPE OF POLYMER
PLASTICIZER
DESCRIPTION OF THE FORMULATION
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
TRIETHYL CITRATE (TEC)
GEL, TRANSPARENT
DL-POLYLACTIC ACID (DL-PLA; I.V. = 0.58)
ACETYL TRIETHYL CITRATE (ATEC)
GEL, SLIGHTLY CLOUDY
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V.= 0.58)
2-PYRROLIDONE
LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
TRIETHYL CITRATE AND POLYETHYLENE GLYCOL 400 (TEC + PEG 400)
GEL
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
ACETYL TRIETHYL CITRATE AND POLYETHYLENE GLYCOL 400 (ATEC+ PEG 400)
GEL
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
TRIETHYL CITRATE (TEC)
GEL, SLIGHTLY YELLOW
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.58)
N-METHYL PYRROLIDONE (NMP)
LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
TRIETHYL CITRATE (TEC)
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. =0.15)
ACETYL TRIETHYL CITRATE (ATEC)
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
TRIETHYL CITRATE (TEC)
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
POLYETHYLENE GLYCOL 400 (PEG-400)
VISCOUS LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. =0.15)
ACETYL TRIETHYL CITRATE AND N-METHYL PYRROLIDONE (NMP) (ATEC + NMP)
LIQUID, TRANSPARENT
DL-POLYLACTIC-CO-GLYCOLIC ACID (DL-PLGA; I.V. = 0.15)
TRIETHYL CITRATE CITRATE AND N-METHYL PYRROLIDONE (NMP) (TEC + NMP)
LIQUID, TRANSPARENT
PCT/USO1/06138
polymer, the desired controlled release of the OTC was achieved in vivo in quail, as seen in Figure 16.
All publications, patents and patent publications mentioned in this specification are herein incorporated by reference into the specification in their entirety for all purposes. Although the invention has been described with reference to preferred embodiments and examples thereof, the scope of the present invention is not limited only to those described embodiments. As will be apparent to persons skilled in the art, modifications and adaptations to the above-described invention can be made without departing from the spirit and scope of the invention, which is defined and circumscribed by the appended claims.
The foregoing is offered primarily for purposes of illustration. It will be readily apparent to those of ordinary skill in the art that the operating conditions, materials, procedural steps and other parameters of the invention described herein may be further modified or substituted in various ways without departing from the spirit and scope of the invention. For example, the invention has been described with human patients as the usual recipient, but veterinary use is also contemplated. Thus, the preceding description of the invention should not be viewed as limiting but as merely exemplary.
>1
Claims (42)
1. A biodegradable vehicle or delivery system comprising: (a) at least one biodegradable polymer, said polymer selected from the group consisting of homopolymers and copolymers or blends thereof, of polyesters, polyphosphoesters, polyorthoesters, polylactic acid or polylactides, polyglycolic acid or polyglycolides, polycaprolactones, polyalkylcyanoacrylates, polyphosphazenes, polyhydroxybutyrates, polyhydroxyvalerates, polyaminoacids, pseudopolyamino acids, polyamides, polyanhydrides, polydioxanone, poly(e-decaloactone), poly(glycolide-co-trimethylene carbonate), poly (ethylene carbonate), poly (iminocarbonate), poly(l, 3-propylene malonate), poly (ethylene-1,4-phenylene-bis-oxyacetate), and poly (ester-amides); and (b) at least one plasticizer; said plasticizer being capable of modulating both the consistency and hydrophobicity or hydrophilicity of said biodegradable vehicle or delivery system.
2. The biodegradable vehicle or delivery system of claim 1 wherein said plasticizer is selected from a group consisting of citrates such as diethyl citrate (DEC), triethyl citrate (TEC), acetyl triethyl citrate (ATEC), tributyl citrate(TBC), acetyl tributyl citrate (ATBC),butyryltri-n-hexyl-citrate, acetyltri-n-hexyl citrate, phthalates such as dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate(DBP), dioctyl phthalate, glycol ethers such as ethylene glycol diethyl ether, propylene glycol monomethyl ether, ethylene glycol monoethyl ether, diethylene glycol monoethyl ether, propylene glycol monotertiary butyl ether, dipropylene glycol monomethyl ether, N-methyl2-pyrrolidone, 2 pyrrolidone, isopropyl myristate, isopropyl palmitate, dimethylacetamide, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, sebacates such as dibutyl sebacate, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, gamma butyrolactone, polyethylene glycols (PEG), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree such as cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids (well defined mixtures of mono esters, diesters, and triesters of glycerol and mono esters, diesters, and triesters of polyethylene glycols with fatty acids of varying chain length, such as caprylic, capric, lauric, palmetic, oleic, linoleic, linolenic, stearic, etc.) such as PEG-6 glycerol mono oleate, PEG-6 glycerol linoleate,PEG-8 glycerol linoleate, PEG-4 glyceryl caprylate/caprate, PEG-8 glyceryl caprylate/caprate, polyglyceryl-3 -oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
3. The biodegradable vehicle or delivery system of claim 1, further comprising at least one biologically active substance.
4. The biodegradable vehicle or delivery system of claim 3 wherein said biologically active substance is selected from the group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), narcotic agonists and antagonists, fertility regulating agents, antibodies and antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, angiogenic and antiangiogenic agents, anti-inflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, live or killed bacteria and viruses, agents or extracts derived from whole or parts of plants, trees, flowers, fruits, buds, seeds, leaves, barks, stem, roots, and animal tissues, growth promoting agents, soft and hard tissues, growth promoting agents, cells, tissues such as bones or agents derived there from, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid (RNA) RNA fragments, and biological tissues such as islets of langerhans and pancreas.
5. A method for the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolmers of polyesters, polyorhoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
6. The method of claim 5 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene gycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate, acetyltri-n-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PECs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
7. The method of claim 5 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antiinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
8. The method of claim 5 comprising at least two biodegradable polymers.
9. The method of claim 5 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
10. The method of claim 9 wherein said biologically active substances are selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antiinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
11. A method for enhancing the stability of the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or polymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, porno acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
12. The method of claim 11 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate, acetyltri-n-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol captylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
13. The method of claim 11 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, algal agents, cavity and infection preventing agents, cardiovascular agents, antinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
14. The method of claim 11 comprising at least two biodegradable polymers.
15. The method of claim 11 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
16. A method for modulating the release characteristics of the biologically active substance in the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
17. The method of claim 16 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol glycerol glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate,acetyltri-n-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6dioleate, polyglyceryl-3isostearate, PEG32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
18. The method of claim 16 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antiinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-in deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
19. The method of claim 16 comprising at least two biodegradable polymers.
20. The method of claim 16 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
21. A method for modulating the dose of a bioactive agent in the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biological active substance-loaded biodegradable vehicle or delivery system.
22. The method of claim 21 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate, acetyltri-n-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6-dioleate, 36 iJl'cLLifCTUnl P Or ;,.z ft;hYY OtriGuj REOPh/cn polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearte, glyceryl palmitostearate, and glyceryl triacetate.
23. The method of claim 21 wherein said biologically active agent is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigen anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents antiinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
24. The method of claim 21 comprising at least two biodegradable polymers.
25. The method of claim 21 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
26. A method for modulating the consistency or rheology of the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates; polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
27. The method of claim 26 wherein said plasticizers are selected from the group consisting of citrates, phhalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate, acetyltri-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6dioleate, polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
28. The method of claim 26 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antiinflammatory agents, vasodilators, brochiodilators, alkaloid, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agent derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
29. The method of claim 26 comprising at least two biodegradable polymers.
30. The method of claim 26 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
31. A method for modulating the hydrophilicity or hydrophobicity of the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group costing of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones, polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
32. The method of claim 31 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol, glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-citrate, acetyltri-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate, propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3 oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate, PEG32 glyceryl laurate, PEG-32 glyceryl palmitostearate, 38 R C. r* t- PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate.
33. The method of claim 31 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural issues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
34. The method of claim 31 comprising at least two biodegradable polymers.
35. The method of claim 31 wherein said biologically active substance loaded biodegradable vehicle comprises at least two biologically agent substances.
36. A method for modulating the degradation of the formulation of a biodegradable vehicle or delivery system comprising mixing: (a) at least one biodegradable polymer or copolymer, said polymer or copolymer selected from the group consisting of homopolymers or copolymers of polyesters, polyorthoesters, polylactides, polyglycolides, polycaprolactones polyhydroxybutyrates, polyhydroxyvalerates, polyamino acids, pseudopolyamino acids, polyamides and polyanhydrides; (b) at least two plasticizers, wherein at least one plasticizer is hydrophilic and at least one plasticizer is hydrophobic; and (c) at least one biologically active substance; to produce a biologically active substance-loaded biodegradable vehicle or delivery system.
37. The method of claim 36 wherein said plasticizers are selected from the group consisting of citrates, phthalates, glycol ethers, n-methyl pyrrolidone, 2 pyrrolidone, propylene glycol glycerol, glyceryl dioleate, ethyl oleate, benzylbenzoate, glycofurol, sorbitol, sucrose acetate isobutyrate, butyryltri-n-hexyl-crate, acetyltri-hexyl citrate, sebacates, dipropylene glycol methyl ether acetate (DPM acetate), propylene carbonate, propylene glycol laurate; propylene glycol caprylate/caprate, caprylic/capric triglyceride, gamma butyrolactone, polyethylene glycols (PEGs), vegetable oils obtained from seeds, flowers, fruits, leaves, stem or any part of a plant or tree including cotton seed oil, soy bean oil, almond oil, sunflower oil, peanut oil, sesame oil, glycerol and PEG esters of acids and fatty acids, polyglyceryl-3-oleate, polyglyceryl-6-dioleate, polyglyceryl-3-isostearate, PEG-32 glyceryl laurate, PEG-32 glyceryl palmitostearate, PEG-32 glyceryl stearate, glyceryl behenate, cetyl palmitate, glyceryl di and tri stearate, glyceryl palmitostearate, and glyceryl triacetate. 39
38. The method of claim 36 wherein said biologically active substance is selected from a group consisting of steroids, hormones, antipsychotic agents, agents that act on the central nervous system (CNS-agents), fertility regulating agents, antibodies, antigens, anesthetics, analgesics, antibiotics, antiviral agents, antineoplastic agents, antifungal agents, cavity and infection preventing agents, cardiovascular agents, antiinflammatory agents, vasodilators, brochiodilators, alkaloids, peptides and proteins, vaccines, growth promoting agents, natural tissues such as bones or agents derived therefrom, bone growth promoting agents such as calcium phosphates, calcium sulfate and hydroxyapatites, whole viable cells and cell-lines, deoxyribonucleic acid (DNA), DNA fragments, ribonucleic acid, and biological tissues.
39. The method of claim 36 comprising at least two biodegradable polymers.
40. The method of claim 36 wherein said biologically active substance-loaded biodegradable vehicle comprises at least two biologically active substances.
41. A biodegradable vehicle or delivery system as claimed in claim 1 substantially as herein described with reference to any example thereof and/or the accompanying drawings.
42. A method as claimed in any one of claims 5, 11, 16, 21, 26, 31 and 36 substantially as herein described with reference to any example thereof and/or the accompanying drawings. END OF CLAIMS
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NZ523385A NZ523385A (en) | 2000-06-28 | 2001-02-26 | Biodegradable vehicles and BAS-loaded delivery systems for use as biodegradable fillers and/or spacers, e.g. artificial skin |
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SE9100610D0 (en) * | 1991-03-04 | 1991-03-04 | Procordia Ortech Ab | BIORESORBABLE MATERIAL FOR MEDICAL USE |
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-
2001
- 2001-02-26 EP EP01918249A patent/EP1299048A4/en not_active Withdrawn
- 2001-02-26 NZ NZ523385A patent/NZ523385A/en unknown
- 2001-02-26 WO PCT/US2001/006138 patent/WO2002000137A1/en not_active Application Discontinuation
- 2001-02-26 CA CA002413157A patent/CA2413157A1/en not_active Abandoned
- 2001-02-26 JP JP2002504922A patent/JP2004511431A/en active Pending
- 2001-02-26 AU AU2001245346A patent/AU2001245346A1/en not_active Abandoned
- 2001-02-26 CN CNB018118518A patent/CN1283215C/en not_active Expired - Fee Related
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EP1299048A1 (en) | 2003-04-09 |
EP1299048A4 (en) | 2005-09-28 |
AU2001245346A1 (en) | 2002-01-08 |
JP2004511431A (en) | 2004-04-15 |
CA2413157A1 (en) | 2002-01-03 |
CN1283215C (en) | 2006-11-08 |
WO2002000137A1 (en) | 2002-01-03 |
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