NZ297334A - Prevention/removal of slime using beta-glucanase and diethylene glycol and optionally another enzyme - Google Patents
Prevention/removal of slime using beta-glucanase and diethylene glycol and optionally another enzymeInfo
- Publication number
- NZ297334A NZ297334A NZ297334A NZ29733495A NZ297334A NZ 297334 A NZ297334 A NZ 297334A NZ 297334 A NZ297334 A NZ 297334A NZ 29733495 A NZ29733495 A NZ 29733495A NZ 297334 A NZ297334 A NZ 297334A
- Authority
- NZ
- New Zealand
- Prior art keywords
- water
- composition
- glycol
- component
- enzyme
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/02—Non-contaminated water, e.g. for industrial water supply
- C02F2103/023—Water in cooling circuits
Description
New Zealand No. 297334 International No. PCT/US95/15500
TO BE ENTERED AFTER ACCEPTANCE AND PUBLICATION
Priority dates: 06.12.1994;05.06.1995;
Complete Specification Filed: 21.11.1995
Classification:^) D21H21/04; B08B17/00; C12S9/00; D21C11/00; C02F3/34; A61L2/18
Publication date: 26 August 1998
Journal No.: 1431
NEW ZEALAND PATENTS ACT 1953
COMPLETE SPECIFICATION
Title of Invention:
Biofilm avoidance and removal process and composition
Name, address and nationality of applicant(s) as in international application form:
BETZDEARBORN INC., 4636 Somerton Road, Trevose, Pennsylvania 19053, United States of America
1 2 9 7 3 3
BIOFILM AVOIDANCE AND REMOVAL PROCESS AND COMPOSITION
The present invention relates to the use of at least one enzyme component from the group consisting of carbohydrases, proteases, lipases and glycoproteases and a short-chained glycol component for the avoidance of slime formation and/or for the removal of biofilm on surfaces of water-bearing systems, in particular of industrial process-water systems. The enzyme component(s) and the glycol component can be added to the water-bearing system either separately, i.e., at different points, or in the form of an enzymatic composition containing the enzyme component(s) and the glycol component.
Industrial process- or operating-water systems, such as e.g., open or closed water-cycle systems of paper factories, in particular cooling-water systems, offer suitable conditions for the growth of microorganisms, with the result that a slime known as biofilm is formed on the surfaces of water-bearing systems. In the case of cooling-water systems in particular, these biofilm deposits can lead to a reduced heat exchange, damage to the joints of pipelines and corrosion within the systems. In this way, adverse effects on process control are possible, which can reduce the efficiency of the industrial process in question or impair product quality. In addition to this, biofilm or slime deposits generally lead to higher energy consumption.
2
Most affected by an increased biofilm formation are industrial processes such as the manufacture of pulp, paper, board and textiles. In the case of paper machines for example, fairly large quantities of water 5 are recirculated in cycle systems called "white water systems" (primary or secondary cycle, i.e., white water I or II) (up to ca. 100 to 1000 m3 water per ton of paper, depending on paper type). The white water,
which contains dispersed pulp, forms an ideal culture 10 medium for the growth of microorganisms.
Biofilm deposits consist primarily of bacteria, in particular gram-negative ones, such as Pseudomonas, Acinetobacter and Aerobacter plus Flavobacterium, Desulfovibrio, Escherichia, Bacillus and Sarcina. 15 The cell-wall structure of gram-negative bacteria is a factor which contributes particularly to slime formation. The cell wall consists of peptidoglycan, which consists of acetyl amino sugars and amino acids plus an outer membrane composed of proteins, 20 lipopolysaccharides and lipoproteins. In contrast, the cell wall of gram-positive bacteria is mostly composed of peptidoglycan and teichonic acids.
Microorganisms also produce extensive slime layers or capsules which vary in their composition. Apart 25 from a few exceptions, the slime produced by the bacteria consists of polysaccharides, such as e.g., dextrans, glucans or polyuronides. The volume of slime produced by a single bacterium can be up to many times the volume of the bacterium.
The deposition of the bacterial slimes can most effectively be controlled with biocides, the effect of
3
these biocides being based on the fact that they kill off the microorganisms in the operating water and thus prevent slime production. However, biocides raise many doubts on ecological groups and, because of their toxicity, pose considerable dangers when handled. For this reason, alternative ways of eliminating biofilm were sought in the past, with particular attention being paid to enzymes.
Although the biofilm matrix can have a heterogeneous composition, it is primarily built up from polysaccharides. Research in the field of slime removal has thus concentx-ated in particular on studies of polysaccharides (carbohydrases). It was recently found that proteases are effective means of eliminating biofilm or slime (EP-A-590 746). The proteases most used at this time are alkaline proteases, derived from various Bacillus strains, which display advantageous stability properties vis-a-vis bases and are proteolytically active.
U.S. 4,684,469 describes a process in which the antimicrobial activity of a biocide is strengthened by a polysaccharide-degrading enzyme.
U.S. 4,936,994 describes a mixture of cellulase, alpha-amylase and a protease for the removal of biofilm, and the use of a mixture consisting of glucanase and protease for slime removal is described in DE 3741583 .
Described in WO 92/13807 is a process for the removal of biofilm in which a mixture is used which consists of at least one acid or alkaline protease, at least one glucoamylase or alpha-amylase and at least
4
one surface-active agent (i.e., a detergent (surfactant)), which destroys the polysaccharide material which surrounds the microorganisms adhering to the surfaces of the water-bearing system.
A feature common to the pure enzyme mixtures or enzymatic compositions used to date is that they often have to be combined with a biocide in order to achieve the degree of effectiveness necessary in practice (cf. e.g., U.S. 5,324,432) .
The object of the present invention is therefore to make available a composition or a process for the avoidance of slime formation and/or for the removal of biofilm on surfaces of water-bearing systems which avoids the disadvantages of conventional biocides but achieves their degree of effectiveness.
According to the invention, the object is achieved in that at least one enzyme component from the group consisting of carbohydrases, proteases, lipases and glycol proteases and a glycol component of the general formula
R2 (-O-R1) n-0-R3
is added to the water-bearing system, whereby n is an integer smaller than 10, R1 is an alkyl residue with 2 25 or 3 carbon atoms and R2 and R3 are in each case independently of each other hydrogen, an alkyl group with 1 to 6 carbon atoms or an aryl group.
The aforementioned alkyl groups of the glycol component can be both linear and branched. Understood 30 in particular by the term aryl are radicals with 6 to
14 carbon atoms which can contain alkyl substituents.
WO 96/17632 PCT/DS95/15500
®ftv»
The alkyl or aryl residues r\ R2 and R3 can in each case be the same or different.
Within tire framework of the present invention,, the enzyme component(s) and the glycol component are 5 preferably added to the water-bearing system at the same time.
According to a preferred embodiment of the invention, the combined addition of enzyme(s) and glycol component takes place in the form of a 10 composition which comprises at least one enzyme component in combination with a glycol component, wherein the at least one enzyme component is beta-glucanase and the glycol component is diethylene glycol; and optionally, at least one other enzyme component selected from the group consisting of 15 carbohydrases, proteases, lipases and glycol proteases.
The composition of the invention preferably contains the glycol component in a concentration of 10 to 80 wt.-% relative to th^j total composition, 20 preferably in a concentratio'n of 20 to 60 wt.-%.
According to a particularly preferred version of the invention, the concentration of the glycol component is 20 to 40 wt.-%.
The quantity of the enzymes in the composition 25 according* to the invention lies between 90 and 1 wt.-%,
preferably between 90 and 20 wt.-%, whereby 50 to 20 wt.-% are most preferred (relative in each case to the weight of the composition in liquid form). The difference to 100 wt.-% of the composition can be made 30 up by water. / S? owcf]
0 7 xl im ieiveo
6
PCT/UB95/15S00
Glucanase (alpha- and beta-glucanase), focosidase or pectinase are preferred as enzymes from the class of the carbohydratases. Esperase® (serine protease,
Novo) or Neutrase® (metallo-protease, Novo) come into consideration in particular in the class of the proteases, Liponase® (acetylesterase) is particularly suitable as a lipase, and endo-glycosidase is preferred among the glycol proteases.
While the aforementioned enzymes alone do not remove biofilm sufficiently, it was surprisingly found that the combination of the enzymes with the aforementioned low-chained glycol component in the form of a composition added to the water-bearing system or by simultaneous use (addition) of enzyme component(s) and glycol component brings with it a dramatic improvement in slime reduction. The formation of new biofilm is also greatly reduced. Since even relatively small quantities of the composition are effective, the quantity of enzyme effectively necessary per liter of process or operating water can be greatly reduced.
It is known in the prior art that, when the pH value of the water is neutral, the marked increase in the bacteria cell count gives rise to increased problems through slime formation, which experience shows can be controlled only with great difficulty even when biocides are used. It was able to show, according to the invention that, even when the pH value of the water is neutral, slime formation can be avoided and/or biofilm on the surfaces of the water-bearing system can be removed if the aforementioned enzyme component(s) and the glycol component are added to the system (again
WO 96/i7t>32
7
PCT/U S95/15500
either separately or in the form of an enzymatic composition).
According to a particularly preferred embodiment of the invention, an enzymatic composition is made available which contains beta-glucanse and diethylene glycol, whereby diethylene glycol is present in a proportion of 10 to 80 wt.-%, preferably 20 to 60 wt.-%, relative to the total composition. In the composition which is particularly preferred according to the invention, beta-glucanse is contained in a proportion of 43 wt.-% ard diethylene glycol in a proportion of 25 wt.-%, the difference to 100 wt.-% being made up by water.
The compositions of the present invention develop surprising effectiveness during the degradation of slime and/or for preventing the formation of slime on surfaces of water-bearing systems. According to the invention, water-bearing systems are understood to be in particular industrial process- or operating-water systems, i.e., open and closed cycles such as e.g., cooling-water cycles. The compositions according to the invention are suitable in particular for use in primary, secondary and/or tertiary cycles of paper factories (i.e., white water I and II, residual waste water; cf. e.g., Ullmanns Encyklopadie der technischen Chemie, 4th Ed., Verlag Chemie, Weinheim, Volume 17, p. 577 et seq.), which are ideal culture media for microorganisms and in which the formation of slime and biofilm deposition represents a major problem. The tendency towards slime formation is at its greatest in the chalk water-bearing cycle, and an inadequate
8
removal of the biofilm leads to a reduction in paper quality, to paper machine operating failures and thus to an increase in operating costs. It has been shown that the composition according to the invention is suitable to a surprising extent for the elimination of these problems.
The composition of the present invention develops its surprising effectiveness in numerous other waterbearing systems such as open or closed water-cycle systems, cooling cycles and the like. As a result of the degradation of slime and the avoidance of the formation of new slime, the durability of the waterbearing systems is improved, with the tendency towards corrosion in particular being greatly reduced.
The present invention also relates to the use of the composition according to the invention for the avoidance of slime formation and/or for the removal of biofilm in water-bearing systems, in particular in industrial process-water systems, in which the composition is added to the system in a quantity of 5 to 20 0 ppm relative to the volume of water to be treated.
The compositions of the present invention are ecologically acceptable, non-toxic and display a clearly higher effectiveness compared with the known enzyme-containing purification compositions. The replacement of conventional, toxic biocides is of most advantage for the aforementioned applications.
Enzymes possess a pH value optimum which is specific to their activity. For the composition according to the invention, depending on the pH value
9
present in the water-bearing system, the pertinent optimal enzymes can be selected 'for the composition according to the invention.
The invention is explained below with reference to examples.
Example
43 wt-% beta-glycanase, 25 wt.-% diethylene glycol and 3 2 wt.-% water were mixed to produce an enzymatic composition (Enzyme formula A).
This composition was used to remove biofilm and to avoid the (re)formation of slime in the water cycle of two paper machines of a paper factory. The paper machines had a total capacity of ca. 180,000 tons/year SC paper. The structure of the water cycles for the paper machines which were used to test the composition according to the invention is diagrammatically represented in Figure 1, where appropriate locations for the metered addition of enzymatic compositions are marked.
Preparatory work for conducting the field trial Installation of dosing apparatus
Several days before the start of the field trial, the Grace Dearborn DCS3 02 0 dosing apparatus was installed. The following feed locations (dosing points) were selected:
1. The composition according to the invention EUR8830 was introduced in a container at the headbox of
WO 96/17632 PCT/US95/15500
paper machine PM1 (white water: the dosing point is designated "SWI" in Table 1 and Figure 1).
2. The biocide formulation I (containing 18 wt.-%
2,2-dibromo-3-nitrilopropionamide (DBNPA)) was fed 5 into the stock "Debro" consistency sensor (the dosing point is designated "stock" in Table 1).
3. The biocide formulation II (containing 45 wt.-% Preventol AS 2 and 2 wt.-% dithiol) was fed into the waste "Debro" consistency sensor (the dosing
point is designated "broke" in Table 1).
Two "gamma/5" pumps (ProMinent) were installed for the addition of the biocide formulations, and a "Vario" pump (ProMinent) was used for the addition of the 15 composition according to the invention. All the pumps were calibrated before the start of the practical testing of the composition according to the invention.
Field trial:
The field trial was started after the machines had been thoroughly cleaned. The dosage quantities during the four cycles of the study are shown in Table 1.
During the first cycle, which lasted 9 days, the composition according to the invention was added to the 25 white water (4x30 minutes per day, 1458 ml/min); the biocides were added into the stock (4 x 30 minutes per day parallel to the formulation according to the invention 6 0 ml/min) and into the broke (3 x 30 minutes per day 75 ml/min). While the biocide dose was reduced 30 by approximately 50% in the second cycle, which lasted
days, the dosage of the composition according to the
11
invention was not changed (4 x, 30 minutes per day, 1458 ml/min). During the third cycle, which lasted 9 days, the dosage of the composition according to the invention was reduced by 50% (4 x 30 minutes per day, 73 0 ml/min). Immediately thereafter, the 4th cycle was started without addition of biocide into the short cycle (i.e., without addition of D7806), the added quantity of the formulation according to the invention being reduced once again by ca. 68% (4 x 30 minutes per day, 500 ml/min). The paper machine was now treated for 20 days with Enzyme formulation A only. A small amount of biocide I was used for broke conservation, not part of the white water.
The outcome of the field trial using the aforementioned enzymatic composition was monitored as follows:
1. The mixture of the planktonic microorganisms populations was measured daily by ATP measurements (Bioscan) and total bacteria counts (Petri film and dipslides) in the white water, in the stock and in the broke. (The ATP measurements are based on the principle that, during the change from ATP to AMP in the presence of luciferin and luciferase, in each case defined quantities of light are emitted per ATP molecule, which are measured by sensitive photometers). The results of the measurements in the white water are shown in Figures 2-8. There was no significant increase in the bacteria cell counts or the "relative light unit" (rlu) values. The figures show the typical
12
microbial activity of a system which displays no problems attributable to slime formation, i.e., in which the formation of slime is effectively suppressed.
2. The machine was checked constantly for slime formation and for the problems associated with it. During the trial, no microbiologically caused problems were observed in the paper machine. Nor was it necessary in any case to stop the paper machine because of slime formation.
3. The paper quality was likewise continuously monitored. The statistical distribution of dark spots and holes in the paper before, during and after the addition of the composition according to the invention was ascertained. It was shown that there is no significant difference, in terms of the number of black spots and holes in the manufactured paper, compared with the paper quality when using biocides (c.f. Figures 9 and
) .
4. After a successful field trial over a period of three months, the paper machine was altered to neutral operation. The paper machine was monitored as already previously (see above).
It was shown that, even with a neutral pH value, the addition of the enzymatic composition prevents the occurrence of problems which are attributable to the formation of slime or biofilm.
The paper machine was constantly monitored, and no biofilm depositions were observed. The machine looked
13
very clean and did not have to be stopped because of microbiological problems, even after the changeover to the neutral pH value.
The determination of the bacteria cell count, the quantitative recording of yeasts and fungi and the ATP measurements in the white water, in the stock and in the broke were carried out at regular intervals. It was found that the bacteria cell count rises initially, finally to achieve an equilibrium. The reduction in the proportion of yeasts and fungi is attributable to the neutral pH value (cf. Figures 11 to 13).
Despite the increase in the bacteria cell count, no problems attributable to slime or biofilm formation were observed when using the enzymatic composition according to the invention. The number of black spots and holes did not increase despite the higher bacteria cell counts, which indicates that the simultaneous addition of the said enzyme component(s) and glycol component is extremely effective according to the invention as regards the suppression of slime formation.
Summary of the Results
The use of the composition according to the invention led to results which are comparable with the use of biocides. The slime deposits were effectively eliminated (even at neutral pH value) and the formation of new biofilm was effectively suppressed. The use of the compositions according to the invention for slime removal thus represents an alternative to the use of biocides in industrial process-water systems which is
14
effective and environmentally compatible, in contrast to biocides, and is also favorable in cost terms.
Holes and cracks in paper machines can be reduced by the compositions according to the invention, and odor problems as well as microbially caused blockages in the water-bearing system can be effectively combated.
Table 1 Dosage Regime - Cycle PM1
Phase 1 (9 days)
Dosing Point
Product
Interval ml/min kg/d broke biozid I
3 x 30
75
7.97
stock biozid II
4 x 30
60
8.21
SWI
enzyme A
4 x 30
1458
192.45
Phase 2 (20 days)
Dosing Point
Product
Interval ml/min kg/d broke biozid I
3 x 30
50
.31
stock biozid II
4 x 30
4.1
SWI
enzyme A
4 x 30
1458
192.45
Phase 3 (9 days)
Dosing Point
Product
Interval ml/min kg/d broke biozid I
3 x 30
50
.31
stock biozid II
4 x 30
4.1
SWI
enzyme A
4 x 30
730
96.36
Phase 4 (20 days)
Dosing Point
Product
Interval ml/min kg/d broke biozid I
3 x 30
50
.31
stock biozid II
SWI
enzyme A
4 x 30
500
65.4
pct/us9s/15500
29731
Claims (18)
1. Composition for the avoidance of slime formation and/or for the removal of biofilm on surfaces of water-bearing systems, characterized in that the composition comprises at least one enzyme component in combination with a glycol component, wherein the at least one enzyme component j.s beta-glucanase and the glycol component is diethylene glycol; and optionally, at least one other enzyme component selected from the group consisting of carbohydrases, proteases, lipases and glycol proteases.
2. Composition according to Claim 1, characterized in that the proportion oZ the glycol component in the total composition is 10 to 80 wt.-%.
3. Composition according to Claim 1, characterized in that the proportion of the enzyme component in the total composition is 90 to 20 wt.-%."
4. Composition according to any one of Claims 1 to 3, characterized in that the carbohydrase is glucanase, fucosiaase or pectinase.
5. Composition according to any one of Claims 1 to 4, characterized in that the protease is Esperase or Neutrase.
6. Composition according to any one of Claims 1 to 5, characterized in that the lipase is Lipolase. glycosidase.
7. Composition according to any one of characterized in that the glycoprotease : WO 96/17632 pct/us95/15500 16
8. Composition according to Claim 1, characterized in that the composition contains 30 to 50 wt.-% beta-glucanase and 20 to 40 wt.-% diethylene glycol.
9. Use of at least one enzyme component with a glycol component, wherein the at least one enzyme component is beta-glucanase and the glycol component, is diethylene glycol; and optionally, at least one other enzyme component selected from the group consisting of carbohydrases, proteases, lipases and glycol proteases for the removal of biofilm and/or for the prevention of slime formation on surfaces of water-bearing systems.
10. Use according to Claim 9, characterized in that the enzyme component(s) and the glycol component are added to the water-bearing system at different points.
11. Use according to Claim 9, characterized in -hat the enzyme components(s) and the glycol component are added to the water-bearing system in the form of an enzymatic composition according to any one of Claims 1 to 8.
12. Use according to Claim 11, characterized in that the enzymatic composition is added in a concentration of 5 to 2 00 ppm.
13. Use according to any one of Claims 9 to 12, characterized, in that the water-bearing system is an open or closed industrial process-water system.
14. Use according to Claim 13, characterized in that the industrial process-water system is an open or closed water cycle in a paper factory. WO 96/17632 PCTAJS95/1SS00 17
15. Use according to Claim 14, characterized in that the water -bearing system is the white water-bearing cycle of a paper factory.
16. Use according to Claim 13, characterized in that the water-bearing system is a cooling cycle.
17. Use according to any one of Claims 9 to 16, substantially as herein described.
18. A composition according to any one of Claims 1 to 8, substantially as herein described. OF CLAIMS
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19944445070 DE4445070A1 (en) | 1994-12-06 | 1994-12-06 | Slime formation and/or bio-film removal compsn. in water-contg. system |
US46521495A | 1995-06-05 | 1995-06-05 | |
PCT/US1995/015500 WO1996017632A1 (en) | 1994-12-06 | 1995-11-21 | Biofilm avoidance and removal process and composition |
Publications (1)
Publication Number | Publication Date |
---|---|
NZ297334A true NZ297334A (en) | 1998-08-26 |
Family
ID=25942968
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NZ297334A NZ297334A (en) | 1994-12-06 | 1995-11-21 | Prevention/removal of slime using beta-glucanase and diethylene glycol and optionally another enzyme |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP0796114A4 (en) |
JP (1) | JPH10509642A (en) |
CN (1) | CN1168637A (en) |
AU (1) | AU695600B2 (en) |
BR (1) | BR9509974A (en) |
CA (1) | CA2203436A1 (en) |
CZ (1) | CZ173897A3 (en) |
FI (1) | FI972388A0 (en) |
NO (1) | NO972499L (en) |
NZ (1) | NZ297334A (en) |
WO (1) | WO1996017632A1 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2818150B1 (en) * | 2000-12-15 | 2004-04-30 | Anios Lab Sarl | COMPOSITION FOR THE TREATMENT OF OBJECTS FOR DISINFECT |
DE102004001590B4 (en) * | 2004-01-09 | 2012-12-06 | Gerhard Knapp | Use of an active substance and a combination of active substances for inhibiting mite faeces and / or mold spores and / or plant pollen and spores and / or for denaturing the keratin of animal hair |
KR101384328B1 (en) * | 2006-03-23 | 2014-04-10 | 가오 가부시키가이샤 | Biofilm formation inhibitor composition |
JP5322400B2 (en) * | 2006-04-21 | 2013-10-23 | 花王株式会社 | Biofilm control agent composition |
CN101448399B (en) * | 2006-04-21 | 2013-06-05 | 花王株式会社 | Composition of biofilm control agent |
CN105753142B (en) * | 2016-04-18 | 2019-03-26 | 南京大学 | A kind of the in-situ activation agent and in-situ activation method of aerating biological filter pool filler biomembrane |
US10478322B2 (en) | 2017-06-19 | 2019-11-19 | Covidien Lp | Retractor device for transforming a retrieval device from a deployed position to a delivery position |
CN111213843A (en) * | 2020-01-30 | 2020-06-02 | 吴高峰 | Production process of instant deoiling beef tendon silk |
CN111150010A (en) * | 2020-01-30 | 2020-05-15 | 吴高峰 | Making process of instant peeled shredded squid |
CN111449205A (en) * | 2020-04-05 | 2020-07-28 | 吴高峰 | Making process of instant barbecue lamp shadow beef tendon |
CN111436567A (en) * | 2020-04-05 | 2020-07-24 | 吴高峰 | Making process of instant spicy lamp shadow beef tendon |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3717550A (en) * | 1970-09-25 | 1973-02-20 | Pabst Brewing Co | Liquid compositions of bacterial protease and/or amylase and preparation thereof |
US3855142A (en) * | 1971-07-15 | 1974-12-17 | Lever Brothers Ltd | Enzymatic denture cleanser |
BE792982A (en) * | 1971-12-20 | 1973-06-19 | Procter & Gamble Europ | Proteolytic enzymes detergent - contg cationic and anionic surfactants |
US4067773A (en) * | 1975-09-02 | 1978-01-10 | William Zinsser & Co. | Enzyme-containing article for removing paper adhered to a surface |
US4684469A (en) * | 1983-05-17 | 1987-08-04 | Ecolab Inc. | Two component biocidal process |
FI75973C (en) * | 1986-12-12 | 1988-09-09 | Kemira Oy | FOERFARANDE FOER ELIMINERING AV MIKROBER I PROCESSVATTEN AV PAPPERSFABRIKER. |
US5206026A (en) * | 1988-05-24 | 1993-04-27 | Sharik Clyde L | Instantaneous delivery film |
US4936994A (en) * | 1989-03-13 | 1990-06-26 | Nalco Chemical Company | Application of cellulase to control industrial slime |
DE69203992T2 (en) * | 1991-02-12 | 1996-03-21 | Buckman Labor Inc | COMPOSITION AND METHOD FOR REMOVING OR AVOIDING BIOBAGAGE. |
CA2106609A1 (en) * | 1992-09-28 | 1994-03-29 | Irene Yeatman Aldridge | Proteases to inhibit and remove biofilm |
US5238572A (en) * | 1993-01-25 | 1993-08-24 | Betz Laboratories, Inc. | Enzyme treatment for industrial slime control |
US5324432A (en) * | 1993-06-17 | 1994-06-28 | Nalco Chemical Company | Treatment of process waters to destroy filamentous bacteria |
-
1995
- 1995-11-21 CN CN95196641A patent/CN1168637A/en active Pending
- 1995-11-21 BR BR9509974A patent/BR9509974A/en not_active Application Discontinuation
- 1995-11-21 WO PCT/US1995/015500 patent/WO1996017632A1/en not_active Application Discontinuation
- 1995-11-21 CZ CZ971738A patent/CZ173897A3/en unknown
- 1995-11-21 NZ NZ297334A patent/NZ297334A/en unknown
- 1995-11-21 AU AU42474/96A patent/AU695600B2/en not_active Ceased
- 1995-11-21 CA CA002203436A patent/CA2203436A1/en not_active Abandoned
- 1995-11-21 EP EP95940866A patent/EP0796114A4/en not_active Withdrawn
- 1995-11-21 JP JP8517645A patent/JPH10509642A/en active Pending
-
1997
- 1997-06-02 NO NO972499A patent/NO972499L/en not_active Application Discontinuation
- 1997-06-05 FI FI972388A patent/FI972388A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
MX9704090A (en) | 1997-09-30 |
AU695600B2 (en) | 1998-08-20 |
CN1168637A (en) | 1997-12-24 |
WO1996017632A1 (en) | 1996-06-13 |
EP0796114A1 (en) | 1997-09-24 |
NO972499L (en) | 1997-06-06 |
BR9509974A (en) | 1998-06-09 |
JPH10509642A (en) | 1998-09-22 |
FI972388A (en) | 1997-06-05 |
EP0796114A4 (en) | 2000-06-14 |
CZ173897A3 (en) | 1998-10-14 |
AU4247496A (en) | 1996-06-26 |
FI972388A0 (en) | 1997-06-05 |
CA2203436A1 (en) | 1996-06-13 |
NO972499D0 (en) | 1997-06-02 |
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