NO993151L - Process for the preparation of purified dimer of bone derived factor - Google Patents

Process for the preparation of purified dimer of bone derived factor

Info

Publication number
NO993151L
NO993151L NO993151A NO993151A NO993151L NO 993151 L NO993151 L NO 993151L NO 993151 A NO993151 A NO 993151A NO 993151 A NO993151 A NO 993151A NO 993151 L NO993151 L NO 993151L
Authority
NO
Norway
Prior art keywords
dimer
derived factor
subjecting
bone
preparation
Prior art date
Application number
NO993151A
Other languages
Norwegian (no)
Other versions
NO993151D0 (en
Inventor
Hidetoshi Andou
Jun Honda
Shunjiro Sugimoto
Original Assignee
Hoechst Marion Roussel Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18445882&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO993151(L) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoechst Marion Roussel Ltd filed Critical Hoechst Marion Roussel Ltd
Publication of NO993151L publication Critical patent/NO993151L/en
Publication of NO993151D0 publication Critical patent/NO993151D0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Fremgangsmåte for fremstilling av en renset dimer av en benavledet faktor, som omfatter underkastelse av et inklusjonslegeme av en benavledet faktor fremstilt ved hjelp av genetisk konstruksjon for følgende trinn a) til. e) i rekkefølge; a) trinnet med behandling av et inklusjonslegeme av en benavledet faktor med et denaturerende middel for å fremstille en oppløst monomer; b) trinnet med behandling av den oppløste monomer med en refoldende oppløsning for å fremstille en dimer; c) trinnet med underkastelse av den refoldende dimer for ultrafiltrering og utbytting av oppløsningsmiddel; d) trinnet med underkastelse av dimeroppløsningen fremstilt ovenfor for isoelektrisk utfelling; og e) trinnet med underkastelse av den isoelektrisk utfelte dimer for revers-fasekromatografi.A process for the preparation of a purified dimer of a bone-derived factor, which comprises subjecting an inclusion body to a bone-derived factor produced by genetic engineering for the following steps a) to. e) in order; a) the step of treating an inclusion body of a bone-derived factor with a denaturing agent to produce a dissolved monomer; b) the step of treating the dissolved monomer with a refolding solution to produce a dimer; c) the step of subjecting the refolding dimer to ultrafiltration and solvent exchange; d) the step of subjecting the dimer solution prepared above for isoelectric precipitation; and e) the step of subjecting the isoelectrically precipitated dimer for reverse phase chromatography.

NO993151A 1996-12-25 1999-06-24 Process for the preparation of purified dimer of bone derived factor NO993151D0 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP35581296 1996-12-25
PCT/JP1997/004784 WO1998029559A1 (en) 1996-12-25 1997-12-24 Process for preparing purified dimer of bone-derived factor

Publications (2)

Publication Number Publication Date
NO993151L true NO993151L (en) 1999-06-24
NO993151D0 NO993151D0 (en) 1999-06-24

Family

ID=18445882

Family Applications (1)

Application Number Title Priority Date Filing Date
NO993151A NO993151D0 (en) 1996-12-25 1999-06-24 Process for the preparation of purified dimer of bone derived factor

Country Status (21)

Country Link
US (1) US6551801B1 (en)
EP (1) EP0949335B2 (en)
KR (1) KR20000062328A (en)
AP (1) AP9901539A0 (en)
AT (1) ATE266095T1 (en)
AU (1) AU5339598A (en)
BR (1) BR9714183A (en)
CA (1) CA2275464A1 (en)
DE (1) DE69728997T3 (en)
DK (1) DK0949335T4 (en)
EA (1) EA199900596A1 (en)
ES (1) ES2216181T5 (en)
HU (1) HUP0002573A2 (en)
IL (1) IL130641A0 (en)
NO (1) NO993151D0 (en)
PL (1) PL334303A1 (en)
PT (1) PT949335E (en)
SI (1) SI0949335T2 (en)
TR (1) TR199901479T2 (en)
WO (1) WO1998029559A1 (en)
ZA (1) ZA9711580B (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7811793B2 (en) * 1996-12-25 2010-10-12 Biopharma Gesellschaft Zur Biotechnologischen Entwicklung Von Pharmaka Mbh Process for preparing purified active monomer of bone-derived factor
US20040019185A1 (en) * 1996-12-25 2004-01-29 Hidetoshi Andou Process for preparing purified active monomer of bone-derived factor
CA2404567A1 (en) * 2000-03-30 2001-10-11 Takeda Chemical Industries, Ltd. Process for producing recombinant protein
AU2003209071B2 (en) * 2002-02-08 2008-08-14 The University Of Erlangen-Nuremberg Formulation comprising bioactive agents and method of using same
EP1542711A4 (en) * 2002-08-13 2009-07-01 Wyeth Corp PEPTIDES AS SOLUBILIZING EXCIPIENTS FOR TRANSFORMING GROWTH FACTOR s PROTEINS
JP4800739B2 (en) * 2005-10-20 2011-10-26 デンカ生研株式会社 Assay medium and assay method
CL2007002615A1 (en) * 2006-09-08 2008-04-18 Wyeth Corp METHODS TO INSULATE OR PURIFY A PRODUCT THAT INCLUDES PLACING THE LINKED PRODUCT IN CONTACT WITH AT LEAST A WASHING SOLUTION THAT INCLUDES ARGININE AND THEN ELECT THE PRODUCT; AND SUCH PRODUCT.
AU2007234612B2 (en) 2006-12-14 2013-06-27 Johnson & Johnson Regenerative Therapeutics, Llc Protein stabilization formulations
US7678764B2 (en) 2007-06-29 2010-03-16 Johnson & Johnson Regenerative Therapeutics, Llc Protein formulations for use at elevated temperatures
US8058237B2 (en) 2007-08-07 2011-11-15 Advanced Technologies & Regenerative Medicine, LLC Stable composition of GDF-5 and method of storage
WO2009129101A1 (en) * 2008-04-14 2009-10-22 Advanced Technologies And Regenerative Medicine, Llc Liquid buffered gdf-5 formulations
US8524662B2 (en) 2010-12-28 2013-09-03 Depuy Mitek, Llc Compositions and methods for treating joints
US8455436B2 (en) 2010-12-28 2013-06-04 Depuy Mitek, Llc Compositions and methods for treating joints
US8398611B2 (en) 2010-12-28 2013-03-19 Depuy Mitek, Inc. Compositions and methods for treating joints
US8623839B2 (en) 2011-06-30 2014-01-07 Depuy Mitek, Llc Compositions and methods for stabilized polysaccharide formulations
US9682099B2 (en) 2015-01-20 2017-06-20 DePuy Synthes Products, Inc. Compositions and methods for treating joints

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1225225A3 (en) * 1988-04-08 2003-01-08 Stryker Corporation Osteogenic devices
CA2141555C (en) * 1992-07-31 2007-03-27 Stryker Corporation Morphogenic protein soluble complex and composition thereof
JP3570558B2 (en) * 1993-05-01 2004-09-29 持田製薬株式会社 DNA fragment, vector containing the same, transformant transformed with the vector, and method for producing protein using the vector
US5385887A (en) * 1993-09-10 1995-01-31 Genetics Institute, Inc. Formulations for delivery of osteogenic proteins
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
PT955313E (en) * 1995-04-19 2006-07-31 Bioph Biotech Entw Pharm Gmbh NEW PROTEIN AND PROCESS FOR ITS PRODUCTION
US5702637A (en) 1995-04-19 1997-12-30 Minnesota Mining And Manufacturing Company Liquid crystal compounds having a chiral fluorinated terminal portion

Also Published As

Publication number Publication date
PL334303A1 (en) 2000-02-14
AP9901539A0 (en) 1999-06-30
ATE266095T1 (en) 2004-05-15
DE69728997T2 (en) 2004-09-02
EP0949335A4 (en) 2003-01-22
BR9714183A (en) 2000-02-29
WO1998029559A1 (en) 1998-07-09
DE69728997D1 (en) 2004-06-09
AU5339598A (en) 1998-07-31
US6551801B1 (en) 2003-04-22
TR199901479T2 (en) 1999-09-21
HUP0002573A2 (en) 2000-12-28
NO993151D0 (en) 1999-06-24
ZA9711580B (en) 1999-09-23
EA199900596A1 (en) 2000-02-28
IL130641A0 (en) 2000-06-01
KR20000062328A (en) 2000-10-25
DK0949335T3 (en) 2004-08-30
DK0949335T4 (en) 2009-03-23
EP0949335A1 (en) 1999-10-13
SI0949335T2 (en) 2009-04-30
DE69728997T3 (en) 2009-07-09
EP0949335B1 (en) 2004-05-06
CA2275464A1 (en) 1998-07-09
PT949335E (en) 2004-09-30
EP0949335B2 (en) 2008-11-19
SI0949335T1 (en) 2004-10-31
ES2216181T3 (en) 2004-10-16
ES2216181T5 (en) 2009-03-01

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