NO970081L - Method of folding proteins such as recombinant hirudin or epidermal growth factor - Google Patents

Method of folding proteins such as recombinant hirudin or epidermal growth factor

Info

Publication number
NO970081L
NO970081L NO970081A NO970081A NO970081L NO 970081 L NO970081 L NO 970081L NO 970081 A NO970081 A NO 970081A NO 970081 A NO970081 A NO 970081A NO 970081 L NO970081 L NO 970081L
Authority
NO
Norway
Prior art keywords
growth factor
epidermal growth
recombinant hirudin
folding proteins
correctly folded
Prior art date
Application number
NO970081A
Other languages
Norwegian (no)
Other versions
NO970081D0 (en
Inventor
Jui Joa Chang
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of NO970081D0 publication Critical patent/NO970081D0/en
Publication of NO970081L publication Critical patent/NO970081L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF] (urogastrone)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

Det er beskrevet en fremgangsmåte for fremstilling av et biologisk aktivt og korrekt foldet protein i nærvær av denatureringsmidler som urea eller guanidinhydro-klorid og separasjon av det korrekt foldede proteinet direkte derfra. Fremgangsmåten kan f.eks. anvendes ved renaturering av rekombinante proteiner som hirudln eller epidermisk vekstfaktor.A method is described for producing a biologically active and correctly folded protein in the presence of denaturants such as urea or guanidine hydrochloride and separating the correctly folded protein directly therefrom. The procedure can e.g. used in the renaturation of recombinant proteins such as hirudln or epidermal growth factor.

NO970081A 1994-07-25 1997-01-09 Method of folding proteins such as recombinant hirudin or epidermal growth factor NO970081L (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP94810437 1994-07-25
PCT/EP1995/002720 WO1996003425A1 (en) 1994-07-25 1995-07-12 Process for folding of proteins like recombinant hirudin or epidermal growth factor

Publications (2)

Publication Number Publication Date
NO970081D0 NO970081D0 (en) 1997-01-09
NO970081L true NO970081L (en) 1997-01-09

Family

ID=8218291

Family Applications (1)

Application Number Title Priority Date Filing Date
NO970081A NO970081L (en) 1994-07-25 1997-01-09 Method of folding proteins such as recombinant hirudin or epidermal growth factor

Country Status (9)

Country Link
EP (1) EP0804461A1 (en)
JP (1) JPH11505507A (en)
AU (1) AU3109795A (en)
CA (1) CA2194177A1 (en)
FI (1) FI970230A0 (en)
IL (1) IL114699A0 (en)
NO (1) NO970081L (en)
WO (1) WO1996003425A1 (en)
ZA (1) ZA956137B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3734634B2 (en) * 1999-03-03 2006-01-11 ヒゲタ醤油株式会社 Protein activation method and apparatus
US7521479B2 (en) 2001-04-16 2009-04-21 Panacea Pharmaceuticals, Inc. Methods of treating prion disease in mammals
AU2003223936A1 (en) * 2002-05-17 2003-12-02 Kobenhavns Universitet Method for purifying denatured proteins having a desired disulfide bond configuration

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR79124B (en) * 1982-12-22 1984-10-02 Genentech Inc
ES8800957A1 (en) * 1985-02-22 1987-12-01 Monsanto Co Method of somatotropin solubilization and naturation.
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
WO1992014832A1 (en) * 1991-02-26 1992-09-03 Ajinomoto Co., Inc. Processes for purifying human bcdf
US5290920A (en) * 1992-04-16 1994-03-01 Allelix Biopharmaceuticals Inc. Method of purifying human epidermal growth factor

Also Published As

Publication number Publication date
NO970081D0 (en) 1997-01-09
EP0804461A1 (en) 1997-11-05
JPH11505507A (en) 1999-05-21
ZA956137B (en) 1996-03-07
FI970230A (en) 1997-01-20
FI970230A0 (en) 1997-01-20
WO1996003425A1 (en) 1996-02-08
AU3109795A (en) 1996-02-22
CA2194177A1 (en) 1996-02-08
IL114699A0 (en) 1995-11-27

Similar Documents

Publication Publication Date Title
EE03590B1 (en) Aerosol forms of peptides and proteins
DE3686654D1 (en) METHOD FOR ISOLATING RECOMBINANT POLYPEPTIDES IN BIOLOGICALLY ACTIVE FORM.
DE69007975T2 (en) FUSION PROTEIN CONSISTING OF GM-CSF AND IL-3.
IL92012A0 (en) Process for the activation of gene-technologically produced proteins expressed in prokaryotes
ATE327254T1 (en) INTERFERON-BETA FUSION PROTEINS AND THEIR USES
GB2302329A (en) Yeast strains
ATE339878T1 (en) MYC-BINDING ZINC FINGER PROTEINS, THEIR PRODUCTION AND THEIR USE
NO970081L (en) Method of folding proteins such as recombinant hirudin or epidermal growth factor
IL82091A (en) Process for renaturing proteins
ATE115146T1 (en) AMPHIPHILIC PEPTIDES AND THEIR USE.
ES2168282T3 (en) RECOMBINANT D-HYDANTOINASE, PROCEDURE FOR PREPARATION AND USE.
ATE261985T1 (en) METHOD FOR PRODUCING PROPERLY FOLDED, BIOLOGICALLY ACTIVE, RECOMBINANT PROTEIN
MX9700658A (en) Process for folding of proteins like recombinant hirudin or epidermal growth factor.
ATE216706T1 (en) PEPTIDES OF THE HIV GAG PROTEIN, THEIR PRODUCTION AND USE
ATE495191T1 (en) METHOD FOR RENATURING RECOMBINANT, DISULFIDE-CONTAINING PROTEINS AT HIGH PROTEIN CONCENTRATIONS IN THE PRESENCE OF AMINES
ATE171711T1 (en) CYCLOHEXAPEPTIDES AND MIXTURES THEREOF, METHOD FOR THE PRODUCTION AND THEIR USE
DE58907746D1 (en) Method for the determination of fructosamine.
ATE141645T1 (en) IN VITRO TREATMENT OF FUSION PROTEINS
WO1997041207A3 (en) Expression of soluble heterologous proteins in bacteria utilizing a thioredoxin/protein expression vector
DE3783425D1 (en) MICROORGANISMS CARRYING FOREIGN PROTEIN PILI, INSULATED PILI, METHOD FOR ELIMINATING PROTEINS USING THIS PILI AND THEIR BENEFITS.
DK2990D0 (en) WHEAT PROTEIN PREPARATION, METHOD OF PREPARING AND USING THEREOF