NO20004753L - Fluorescent assay for topoisomerase-inhibitorer - Google Patents
Fluorescent assay for topoisomerase-inhibitorerInfo
- Publication number
- NO20004753L NO20004753L NO20004753A NO20004753A NO20004753L NO 20004753 L NO20004753 L NO 20004753L NO 20004753 A NO20004753 A NO 20004753A NO 20004753 A NO20004753 A NO 20004753A NO 20004753 L NO20004753 L NO 20004753L
- Authority
- NO
- Norway
- Prior art keywords
- topoisomerase inhibitors
- fluorescent assay
- fluorescence
- liquid
- topoisomerase
- Prior art date
Links
- 239000003534 dna topoisomerase inhibitor Substances 0.000 title abstract 2
- 229940044693 topoisomerase inhibitor Drugs 0.000 title abstract 2
- 238000003556 assay Methods 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 abstract 1
- 101710183280 Topoisomerase Proteins 0.000 abstract 1
- 239000000980 acid dye Substances 0.000 abstract 1
- -1 cyanine nucleic acid Chemical class 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/99—Isomerases (5.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Det er beskrevet metoder for å bestemme aktiviteten av for- søksforbindelser som topoisomerase-inhibitorer omfattende å blande i en væske, en forsøksforbindelse, et DNA-materiale og en topoisomerase, inkubere væsken, derpå inkorporere et cyanin nukleinsyre-fargestoff så som "PicoGreen", måle flu- orescensen og sammenligne fluorescensen fra kontroller.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7926598P | 1998-03-25 | 1998-03-25 | |
| PCT/IB1999/000473 WO1999049077A1 (en) | 1998-03-25 | 1999-03-22 | Fluorescent assay for topoisomerase inhibitors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| NO20004753D0 NO20004753D0 (no) | 2000-09-22 |
| NO20004753L true NO20004753L (no) | 2000-11-23 |
Family
ID=22149463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO20004753A NO20004753L (no) | 1998-03-25 | 2000-09-22 | Fluorescent assay for topoisomerase-inhibitorer |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1086242A1 (no) |
| JP (1) | JP2002507433A (no) |
| AU (1) | AU3269999A (no) |
| CA (1) | CA2324344A1 (no) |
| IL (1) | IL138532A0 (no) |
| NO (1) | NO20004753L (no) |
| WO (1) | WO1999049077A1 (no) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1328658A2 (en) * | 2000-06-22 | 2003-07-23 | San Diego State University Foundation | Recombination modulators and methods for their production and use |
| GB0424953D0 (en) | 2004-11-11 | 2004-12-15 | Plant Bioscience Ltd | Assay |
| WO2010044101A1 (en) * | 2008-10-08 | 2010-04-22 | V. B. Medicare Pvt. Ltd. | Purification and assay for topoisomerases and use of the assay for screening modulators of topoisomerases |
| WO2012109617A2 (en) * | 2011-02-10 | 2012-08-16 | University Of Central Florida Research Foundation, Inc. | Method of assaying dna topoisomerases and dna binding proteins using high throughput screening |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1992020813A1 (en) * | 1991-05-17 | 1992-11-26 | Dana Farber Cancer Institute | Assays for factors affecting circularization of dna, assays for factors affecting dna integration, factors, and uses thereof |
| WO1996021156A1 (en) * | 1995-01-04 | 1996-07-11 | Abbott Laboratories | Method for preparing scintillation proximity assay targets |
-
1999
- 1999-03-22 JP JP2000538035A patent/JP2002507433A/ja not_active Withdrawn
- 1999-03-22 IL IL13853299A patent/IL138532A0/xx unknown
- 1999-03-22 CA CA002324344A patent/CA2324344A1/en not_active Abandoned
- 1999-03-22 EP EP99942591A patent/EP1086242A1/en not_active Withdrawn
- 1999-03-22 WO PCT/IB1999/000473 patent/WO1999049077A1/en not_active Ceased
- 1999-03-22 AU AU32699/99A patent/AU3269999A/en not_active Abandoned
-
2000
- 2000-09-22 NO NO20004753A patent/NO20004753L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1086242A1 (en) | 2001-03-28 |
| IL138532A0 (en) | 2001-10-31 |
| AU3269999A (en) | 1999-10-18 |
| CA2324344A1 (en) | 1999-09-30 |
| NO20004753D0 (no) | 2000-09-22 |
| WO1999049077A1 (en) | 1999-09-30 |
| JP2002507433A (ja) | 2002-03-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC2A | Withdrawal, rejection or dismissal of laid open patent application |