NL2032971B1 - Molecular marker for identifying trait of efficiency of duck feed utilization based on neuropeptide y5 receptor npy5r gene, method and use thereof - Google Patents
Molecular marker for identifying trait of efficiency of duck feed utilization based on neuropeptide y5 receptor npy5r gene, method and use thereof Download PDFInfo
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Abstract
The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide Y5 receptor NPY5R gene, a method 5 and use thereof. The NPY5R gene has a nucleotide sequence shown in SEQ ID NO: 1, the molecular marker is T or C, and the molecular marker is located at position 782 of the nucleotide sequence. In the present disclosure, mutations of the NPY5R gene are detected using a polymerase chain reaction-single strand conformation polymorphism (PCR—SSCP) method, the traits of efficiency of duck feed utilization are selected based 10 on genotypes, and a breeding method for early selection of efficiency of poultry feed utilization is established. The method is simple, rapid, and low-cost, does not need special instrument, and satisfies experimental needs.
Description
MOLECULAR MARKER FOR IDENTIFYING TRAIT OF EFFICIENCY OF
DUCK FEED UTILIZATION BASED ON NEUROPEPTIDE YS RECEPTOR
NPY5R GENE, METHOD AND USE THEREOF
[0001] The present disclosure relates to the technical field of molecular markers, in particular to a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide YS receptor NPYSR gene, a method and use thereof.
[0002] Feed efficiency trait is one of the important economic traits in livestock and poultry production, among which feed conversion ratio (FCR) and residual feed intake (RFI) are one of the indicators for evaluating feed efficiency. FCR refers to how many products are obtained by feeding a unit of feed (Liang S, Guo Z, Tang J, et al. Genomic divergence during artificial selection by feed conversion ratio in Pekin ducks[J]. Anim
Biotechnol, 2021: 1-9). With the in-depth exploration of feed efficiency, the researchers defined the difference between the actual intake of feed consumed by livestock and poultry in a certain period of time and the expected feed requirements for growth and maintenance thereof as the RFI (Koch RM, Swiger LA, Chambers D, et al.
Efficiency of feed use in beef cattle[J]. Journal of Animal Science, 1963, 22(2):486-494.). Efficiency of poultry feed utilization can be assessed using RFI and
FCR, but there are a plurality of factors that affect the feed efficiency, including feed intake and genetic factors (Pan YZ. Differentiation selection for pig feed efficiency and genome-wide association analysis[D]. Jilin University, 2016; Zhang XX, Study on production performance, rumen microflora and liver transcriptome of lambs with different residual feed intake[D]. Lanzhou University, 2019.).
[0003] Studies have found that neuropeptide Y and its YS receptor NP5YR mediate animal intake behavior and are also related to energy metabolism, which belong to one of the members of the G protein-coupled receptor superfamily (Michel M C,
Beck-Sickinger A, Cox H, et al. XVI International Union of Pharmacology recommendations for the nomenclature of neuropeptide Y, peptide YY, and pancreatic polypeptide receptors[J]. Pharmacol Rev, 1998, 50(1): 143-150.). Tang-Christensen et al. used an oligonucleotide (ODN) antisense to NPY5R to study the effects of NPY5R and NPY on food intake. Through repeated central administration, they found that
ODN significantly reduced spontaneous food intake, resulting in significant weight loss (Tang-Christensen et al. Christensen M, Kristensen P, Stidsen C E, et al. Central administration of Y5 receptor antisense decreases spontaneous food intake and attenuates feeding in response to exogenous neuropeptide Y[J]. J Endocrinol, 1998, 15 9(2):307-12.). In duck studies of individuals with different RFI, researchers detected higher mRNA expression of NP} and NPY5R in the hypothalamus of individuals with high RFI (Zeng T, Chen L, Du X, et al. Association analysis between feed efficiency studies and expression of hypothalamic neuropeptide genes in laying ducks[J]. Anim
Genet, 2016, 47(5): 606-609.). Therefore, it is speculated that NPYSR is involved in the regulation of duck feed efficiency, which provides a conjecture about the relationship between this gene polymorphism and poultry feed efficiency traits for the study of duck feed efficiency.
[0004] In addition, Chinese Patent Application No. CNI111676295A discloses a research method for genes related to feed intake regulation, and specifically discloses the design of primers for polymorphism detection based on the known duck CCK,
CCKAR, NPY and NPY3R genes in NCBI GenBank, for use in SNP loci developed based on the above genes and related to duck feed intake. The results have shown that
CCKAR gene has SNP loci related to duck feed intake, but SNP loci of CCK, NPY and
NPYSR genes have not been reported yet. At present, there is no other literature about the fact that NPY5R and SNP loci related to duck feed intake have been developed.
[0005] In this study, in order to improve the duck feed efficiency, fundamentally reduce the costs laid down, and increase the income of the duck industry, early researchers often used conventional breeding, but the efficiency was not high. As the technology evolves, molecular marker-assisted selection leaps to the eyes, which can fundamentally improve traits genetically, thereby accelerating genetic progress. Based on the above content, a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide Y5 receptor NPY5R gene, a method and use thereof are proposed.
[0006] An objective of the present disclosure is to overcome the deficiencies in the prior art, and to provide a molecular marker for identifying traits of efficiency of duck teed utilization based on a neuropeptide Y5 receptor NPY5R gene, a method and use thereof. Aiming to single nucleotide polymorphism (SNP) molecular markers of candidate genes related to the traits of efficiency of duck feed utilization, the present disclosure solves the problem of slow progress in conventional phenotypic breeding and realizes early identification of the traits of efficiency of feed utilization.
[0007] The present disclosure realizes the above objective through following technical solutions:
[0008] The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide Y5 receptor NPY5R gene.
The NPY5R gene has a nucleotide sequence shown in SEQ ID NO: 1, the molecular marker is T or C, and the molecular marker is located at position 782 of the nucleotide sequence.
[0009] The present disclosure further provides use of the molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide Y5 receptor NPY3R gene in the identification of the traits of efficiency of duck feed utilization.
[0010] The present disclosure further provides a method for identifying traits of efficiency of duck feed utilization using the foregoing molecular marker, including the following steps:
[0011] step 1, extracting total DNA from the venous blood of duck wings;
[0012] step 2, designing primers for specific amplification using a sequence composed of a site where the molecular marker is located and upstream and downstream bases as a target sequence; with the total DNA as a template, using the primers for specific amplification to conduct PCR amplification to obtain a PCR product;
[0013] step 3, genotyping and sequencing the PCR product to obtain a molecular marker type of a duck to be tested; and
[0014] step 4, determining the traits of efficiency of duck feed utilization based on the molecular marker type.
[0015] A further improvement is that lengths of upstream and downstream PCR products at the site where the molecular marker is located are between 200 and 250 bp.
[0016] A further improvement is that sequences of the primers for specific amplification are as follows:
[0017] SEQ ID NO: 2: Forward primer: ATTCTTCTTT GAGTTAGGCA; and
[0018] SEQ ID NO: 3: Reverse primer: GCAGACAGAC AGGGTCCGAG.
[0019] A further improvement is that the genotyping is implemented by conducting native polyacrylamide gel electrophoresis and silver staining on the PCR product to acquire an image and genotyping based on the image, where the PCR product is:
[0020] (1) CC genotype on conditions that there are two bands having a close spacing;
[0021] (2) TT genotype on conditions that there are two bands having a wide spacing; and
[0022] (3) TC genotype on conditions that there are four bands.
[0023] A further improvement is that specific steps of determining the traits of efficiency of duck feed utilization based on the molecular marker type in step 4 are as follows:
[0024] (1) the traits of efficiency of duck feed utilization being extremely high on conditions that the molecular marker type of the duck to be tested is CC genotype;
[0025] (2) the traits of efficiency of duck feed utilization being poor on conditions that the molecular marker type of the duck to be tested 1s TT genotype; and
[0026] (3) the traits of efficiency of duck feed utilization being medium on conditions that the molecular marker type of the duck to be tested is TC genotype.
[0027] The present disclosure has the following beneficial effects: The present disclosure provides a molecular marker for identifying traits of efficiency of duck feed utilization based on a neuropeptide Y5 receptor NPY3R gene, a method and use thereof.
Mutations of the NPYSR gene are detected using a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) method, the traits of efficiency of duck feed utilization are selected based on genotypes, and a breeding method for early selection of efficiency of poultry feed utilization is established. The method is simple, rapid, and low-cost, does not need special instrument, and satisfies experimental needs.
[0028] FIG. 1 illustrates agarose gel electrophoresis of DNA of some samples;
[0029] FIG. 2 illustrates agarose gel electrophoresis of PCR products of some samples;
[0030] FIG. 3 illustrates polyacrylamide gel electrophoresis of PCR products of some samples;
[0031] FIG. 4 illustrates sequencing of individuals with different genotypes at position 782 of a duck NPY5R genome. 5
[0032] The present application will be described in further detail below with reference to the accompanying drawings. It should be pointed out herein that the following specific implementations are only intended to further explain the present application, rather than to be construed as limiting the protection scope of the present application. Those skilled in the art may make non-essential improvements and adjustments to the present application based on the above content.
[0033] 1. Materials
[0034] All methods used in the examples are conventional methods known to those skilled in the art, unless otherwise specified; all materials and reagents used are commercially available, unless otherwise specified.
[0035] 2. Method
[0036] 2.1 Obtaining polymorphic loci of duck NPY3R gene
[0037] 2.1.1 Genomic DNA extraction and detection
[0038] Three hundred and eighty-eight white meat-type ducks were selected, the blood was collected from the wing vein, and total DNA was extracted from the venous blood samples of duck wings using the Blood Genome DNA Extraction Kit produced by Takara Biotechnology (Dalian) Co., Ltd. Specific operations followed the instructions of the kit.
[0039] DNA concentration and OD value were measured with NanoDrop2000. DNA was detected by 1.5% agarose gel electrophoresis. The results are shown in FIG. 1. The quality of the extracted genomic DNA was good, and the main band was single and clear.
[0040] 2.1.2 Primer design
[0041] The DNA sequence corresponding to the NPYSR gene shown in SEQ ID NO: 1 was found from the duck genome database. The partial DNA sequence of the NPY5R gene shown in SEQ ID NO: 1 was used as a template. During the primer design, attention should be paid to arrange the SNP locus in the middle position as much as possible, so as to avoid the occurrence of hairpin structures, primer dimers and mismatches and optimize the primer sequences. The primer sequences are shown as follows:
[0042] SEQ ID NO: 2: Forward primer: ATTCTTCTTT GAGTTAGGCA; and
[0043] SEQ ID NO: 3: Reverse primer: GCAGACAGAC AGGGTCCGAG.
[0044] The length of the amplified fragment of the primer was 236 bp, and the sequence is shown in SEQ ID NO: 4, containing the molecular marker locus of T/C mutation.
[0045] 2.1.3 PCR amplification
[0046] Using Mix produced by Sangon Biotech (Shanghai) Co., Ltd, the target fragment of the NPY5R gene was subjected to PCR amplification through the synthesized sequencing-specific primer pair. The PCR amplification system was as follows:
[0047] © Component Volume ~ DNA template ~~ 08uL
Forward primer 0.1 uL
Reveres primer 0.1 ul
Mix 7 uL ddH:0 7 uL
Total 15 uL
[0048] The PCR amplification program was as follows: initial denaturation at 94°C for 5 min; 34 cycles of denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and extension at 72°C for 30 s; extension at 72°C for 10 min; and final storage at 4°C.
[0049] 2.1.4 Detection of PCR products
[0050] The PCR products were detected by 1wt% agarose gel electrophoresis, and a band with a length of 200-250 bp was obtained after imaging on a gel imager, which was consistent with the predicted length, indicating that the target fragment was obtained. The PCR product was submitted to Sangon Biotech (Shanghai) Co., Ltd. for sequencing. The sequence is shown in SEQ ID NO: 4, which is consistent with the predicted result.
[0051] 2.1.5 Denaturation of PCR products and SSCP detection
[0052] The PCR products are first denatured and then subjected to polyacrylamide gel electrophoresis (PAGE), and finally their mutations were determined according to the results of different banding patterns. The specific steps were as follows:
[0053] (1) A native polyacrylamide gel was prepared according to the instructions.
The native acrylamide gel system is as follows:
[0054] ~~ Aer/Bissolution
Concentratio "Degree of IB dd APS TEME 1 erosslinkin Concentratio Consumptio | E | oO ul) D 2 n (%) n (mL) (mL) (mL) (ul) —_— 12 29:1 30 14 7 14 5 50
[0055] (2) TEMED was finally added, and poured into the mold immediately after adding (make sure that the mold is closed before pouring to prevent gel leak when pouring, and that the size of the gel strip is consistent with that of the comb); the mold was tilted at an angle of about 45° and the gel liquid was poured slowly from the center of the vertical slab (which can effectively avoid the generation of bubbles); the pouring was stopped when it was about away from the upper edge of the mold; a comb prepared in advance was inserted, the gel was polymerized at room temperature for 40 min, and the excess acrylamide was stored at 4°C; the gel polymerization on the glass plate was observed at any time, and an appropriate amount of native acrylamide gel system mixture was replenished.
[0056] (3) A slab was prepared during the gel polymerization waiting process. After the polymerization was completed, glass plates were mounted, IxTBE was added to the slab, so that the TBE solution exceeded the sample wells by about 3 cm, and the air bubbles were removed therefrom.
[0057] (4) 3 uL of PCR product was pipetted into a PCR tube, supplemented with 7 uL of denaturing reagent, briefly centrifuged to mix well, and denatured at 98 °C for 10 min; the PCR tube was quickly taken out and put in a -20°C ice box for 10 min, and the sample was loaded with a 10 uL pipette.
[0058] (5) The power was turned on, the electrophoresis was run at 220 V for 10 min, then the voltage was modulate to 120 V, and the electrophoresis was run for 21 h.
[0059] (6) After electrophoresis, the electrophoresis apparatus was turned off, the glass plates were taken out, and the gel was carefully removed and put in a white porcelain dish filled with clean water to wash once or twice.
[0060] (7) The gel was placed in the dye solution and shaken gently in the dark for 15 min. The dye solution was composed of silver nitrate and pure water, and the concentration of silver nitrate was 0.2%.
[0061] (8) After dyeing, AgNO; was recovered, the gel was washed 1-3 times with deionized water, for 2 min each time, and the excess dye solution was washed away.
[0062] (9) Bands were developed with colorimetric solution to make them clear and the background light yellow. The dyeing could not stop until the bands were clearly visible, and the colorimetric solution was discarded immediately after the color development. The colorimetric solution was 500 mL in volume, containing 2% NaOH + 0.04% Na2CO: + 420 pL formaldehyde.
[0063] (10) A picture was taken and saved.
[0064] 2.1.6 Genotyping
[0065] The PCR-SSCP is shown in FIG. 2. Different banding patterns indicate different genotypes. It can be seen from FIG. 2 that there are three genotypes at position 782 of the NPY5R gene, namely TT, TC, and CC. The CC genotype is two bands having a close spacing, the TT genotype has two bands having a wide spacing, and the TC genotype has four bands.
[0066] 2.1.7 DNA validation and sequencing
[0067] Colorimetric genotyping gel maps were counted to obtain three genotypes, TT,
TC, and CC. One individual was selected for sequence alignment of these three genotypes, respectively. The sequence alignment is shown in FIG. 3; in the sequencing results, T is mutated to C, and the arrow marks the mutation site, which is consistent with the PCR-SSCP result.
[0068] 2.2 Association analysis between the NPYS5R gene T782C mutation site and the traits of efficiency of duck feed utilization
[0069] 2.2.1 Genotyping
[0070] In order to determine the association between the T/C polymorphism at position 782 of the NPY5R gene and duck important traits, 388 white meat-type ducks were used as experimental materials, which were recorded from hatching, labeled with wing tags, and raised normally. The RFI, individual daily feed intake (FI), average daily gain (ADG), feed conversion ratio (FCR), and metabolic body weight (MBW) were calculated for 388 white meat-type ducks aged 21-42 days. Using the genotyping method of Section 2.1.6, 388 ducks were genotyped. The results are shown in Table 1.
[0071] Table 1 Genotyping results of individuals with different phenotypes
[0072] © Genotype frequency Gene frequency Chi-square test
Locus "1 TC CC n= n= 106 T C x? P value 228 134
[0073] The chi-square test showed that the genotypes of the experimental duck population were in Hardy-Weinberg equilibrium.
[0074] 2.2.2 Statistical analysis
[0075] The numbers of TT, TC and CC genotypes were counted by gel map, and the differences between the three genotypes and slaughter performance were analyzed by
One-way ANOVA in SPSS20.0. The results of association analysis between different genotypes and various traits are shown in Table 2:
[0076] Table 2 Analysis association between duck NPYSR genotypes and traits of efficiency of duck feed utilization
[0077]
Genotype
Trait eee eee eee eee eee eee eee eee eee eee
TT (1 = 228) TC (n= 134) CC (n=26) © BW42(g) 385652427712 3824.74+30424 385538422437
FI (g) 2723142454 266.39+25.58 264.18+17.92
BWG (g) 133.07+12.05 131.06£13.47 131.37+10.30
MBW95 349.08+17.32 347.92+19.02 350.92+13.41
FCR 2.05+0.14 2.04£0.15 2.02+0.13
RFI (g/day) 1.75+15.89° —1.80+13.58% 6.01+13.77°
[0078] NOTE: Different lowercase letters in the same row indicate significant difference (P < 0.05), and no letter indicates no significant difference (P > 0.05).
[0079] As can be seen from the table, through the comparison of feed efficiency traits of individuals with different genotypes, CC individuals were significantly lower than
TT ones in terms of RFI (P < 0.05); while in terms of FL BW42, BWG, and FCR, the above three genotypes were not significantly different. Since RFI is a negative selection trait, it is concluded that individuals with CC genotype have extremely high feed conversion traits, those with TC genotypes have medium feed conversion traits, and those with TT genotypes have poor feed conversion traits.
[0080] The above examples merely represent several embodiments of the present disclosure, and the descriptions thereof are specific and detailed, but they should not be construed as limiting the patent scope of the present disclosure. It should be noted that those of ordinary skill in the art can further make several variations and improvements without departing from the concept of the present disclosure, and all of these fall within the protection scope of the present disclosure.
Sequence Listing Information:
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Software Name: WIPO Sequence
Software Version: 2.0.0
Production Date: 2022-08-04
General Information:
Current application / Applicant file reference: HKJP20220700217
Earliest priority application / IP Office: CN
Earliest priority application / Application number: 202210499499 .X
Earliest priority application / Filing date: 2022-05-09
Applicant name: Anhui Agricultural University
Applicant name / Language: en
Invention title: MOLECULAR MARKER FOR IDENTIFYING TRAIT OF
EFFICIENCY OF DUCK FEED UTILIZATION BASED ON NEUROPEPTIDE Y5
RECEPTOR NPY5R GENE, METHOD AND USE THEREOF (en)
Sequence Total Quantity: 4
Sequences:
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Length: 1500
Molecule Type: DNA
Features Location/Qualifiers: - source, 1..1500 > mol type, other DNA > note, NPY5R gene of duck > organism, synthetic construct
Residues: aaaaaataaa taaataagag aaagcatccc ttaaatacag gcgagcgcgg gatttgttta 60 cagtctcact gcatttccat ggaaactcta attgtcagag catgcagcag ggaaataata 120 ggctccagac cccgcacgga gcgctctccc gaaatgcacc cgcgcggcgg ccggctgagc 180 acccagcacc gagcacccag caccgcagca gcagcagctg ccgggtgcct ggccctggcg 240 cctccgcagc acccgcataa gatgcgctgc cgggcatcct cgccctgctc ccgcagagcc 300 gtgccggggc tatgagcggt gcctgggggc tgcgcactcg cggccccttc tgcagcgggc 360 tcccegtgge tgcaggtgag cagggggceag Cgggstggge aggsggcasa ggtgtaaatt 420 tgcatctaag tgccgaggcc gcgcagcggg tgcagaaagc ccaggtttca cttcagtgcg 480 ggiggccgag agggctgigg aagggcaaaa ggatgccagg gtgggiggst gactgtccca 540 cctggttttg tgaatccatc ctttgctgac cccacgccac aggatgcagg agcgcatccc 600 aagtttgctg cttctggcca gcaaaggccc cgctaagttg cctcgcactt ccttggagct 660 ggggaaataa tctcattaga tggcttttgg ttgttttgge aagccatgtt ctcgtgttcc 720 gggataatgt tgcaattctt ctttgagtta ggcatgtcaa gagacctatc actagcttgc 780 atgaaaatca atatttctgg gcttgtgaag tgagctgaat cttttgaact ctcccggctc 840 catggacaag cttttgctgt tgcacgtaaa gagcagctgc stttgcagca gcagcatttg 900 atcttcagac cgtgctgtga acacagctca tcggtggttt tggtgteggg ctcggaccct 960 gtctgtctgc tgagatggeg cacagttgct caggtagtaa cgacacagct cagtgcttcc 1020 cttaagctta ggtegtcagg cagcgcagcc cagctcatta gcagaatttt acaacacaga 1080 agttataaaa gactctgaca ttttttttcc tatacactcc agtaatacag — ggttgggesg 1140 ctgtgtttca aagggatttt ttgtgttttg ctttetccet gtatatcctc tcctgctgtt 1200 aaggtagagg aaggacttcc aagactaaga tgctctcagc agtgaagata aattgtcctg 1260 gacaggctct ctgacataga ctgaacagac tgtacaggca ccagccagtt agttatttta 1320 ataggtatta atatcgtagg atacatgatc ttatccacaa ttaagcctta atatgagcat 1380 ctcatctagc agaaggaaga tctcctgaca gagcaaatgc cttcagtgga ggatggctgc 1440 cagcctaact tgcgtattcc tgacaatttc catttaaata tgtaaagaaa cagaaatagc 1500
Sequence Number (ID): 2
Length: 20
Molecule Type: DNA
Features Location/Qualifiers: - source, 1..20 > PCR primers, fwd _name:Forwardprimer,fwd seq:attcttctttgagttaggca,rev name:Reverseprimer,rev $ eq:gcagacagacagggtccgag > mol type, other DNA > note, Forward primer > organism, synthetic construct
Residues: attcttcttt gagttaggca 20
Sequence Number (ID): 3
Length: 20
Molecule Type: DNA
Features Location/Qualifiers: - source, 1.20 > PCR primers, fwd name:Forwardprimer,fwd seq:attcttctttgagttaggca,rev name:Reverseprimer,rev s eq:gcagacagacagggtccgag > mol type, other DNA > note, Reverse primer > organism, synthetic construct
Residues: gcagacagac agggtccgag 20
Sequence Number (ID). 4
Length: 237
Molecule Type: DNA
Features Location/Qualifiers: - source, 1.237 > mol type, other DNA > note, PCR product amplified by target fragment of NPY5R gene > organism, synthetic construct
Residues: aagccatgtt ctcgtgttcc gggataatgt tgcaattctt ctttgagtta ggcatgtcaa 60 gagacctatc actagcttgc atgaaaatca atatttctgg gettgtgaag tgagctgaat 120 cttttgaact ctcccggctc catggacaag cttttgctgt tgcacgtaaa gagcagctgc 180 gtttgcagca gcagcatttg atcttcagac cgtgctgtga acacagctca tcggteg 237
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| CN116574812B (en) * | 2023-05-15 | 2024-05-17 | 安徽农业大学 | Molecular marker for identifying duck feed utilization rate character based on ELOVL2 gene, identification method and application thereof |
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| CN108165639A (en) * | 2018-03-12 | 2018-06-15 | 中国农业科学院北京畜牧兽医研究所 | A kind of and the relevant molecular labeling of fleshy duck fodder transformation efficiency character and its application |
| CN111676295A (en) | 2020-05-28 | 2020-09-18 | 浙江省农业科学院 | Research method of gene related to feed intake regulation |
| CN114703299A (en) * | 2022-05-09 | 2022-07-05 | 安徽农业大学 | Molecular marker for identifying duck feed utilization rate character based on neuropeptide Y5 receptor NPY5R gene, and method and application thereof |
| CN114703298A (en) * | 2022-05-09 | 2022-07-05 | 安徽农业大学 | Molecular marker for identifying duck feed utilization rate character based on neuropeptide gene NPY and method and application thereof |
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| US7873482B2 (en) * | 2008-12-16 | 2011-01-18 | Bruno Stefanon | Diagnostic system for selecting nutrition and pharmacological products for animals |
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| CN108165639A (en) * | 2018-03-12 | 2018-06-15 | 中国农业科学院北京畜牧兽医研究所 | A kind of and the relevant molecular labeling of fleshy duck fodder transformation efficiency character and its application |
| CN111676295A (en) | 2020-05-28 | 2020-09-18 | 浙江省农业科学院 | Research method of gene related to feed intake regulation |
| CN114703299A (en) * | 2022-05-09 | 2022-07-05 | 安徽农业大学 | Molecular marker for identifying duck feed utilization rate character based on neuropeptide Y5 receptor NPY5R gene, and method and application thereof |
| CN114703298A (en) * | 2022-05-09 | 2022-07-05 | 安徽农业大学 | Molecular marker for identifying duck feed utilization rate character based on neuropeptide gene NPY and method and application thereof |
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