NL2030710A - Cell product quality testing and collection device - Google Patents

Cell product quality testing and collection device Download PDF

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Publication number
NL2030710A
NL2030710A NL2030710A NL2030710A NL2030710A NL 2030710 A NL2030710 A NL 2030710A NL 2030710 A NL2030710 A NL 2030710A NL 2030710 A NL2030710 A NL 2030710A NL 2030710 A NL2030710 A NL 2030710A
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gear
main
pinion
semi
main gear
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NL2030710A
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Dutch (nl)
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NL2030710B1 (en
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Meng Chunyan
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Univ North China Science & Technology
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a cell product quality testing and collection. device, comprising a sterile table, inside of whose lower part an motor is mounted and at whose inner side a placement seat is connected in a clamped way, and a main rotating rod, whose lower end is connected to the output end of the said motor and to whose outer side a semi—gear is connected through a key, with a main gear engaged to the right of the said semi—gear, a secondary rotating rod connected through a key to the inner side of the said main gear, and a pinion connected through a key to the outer side above the said secondary rotating rod. This process only requires the staff to drive the piston. move upward, and, downward, which operation is convenient.

Description

P1108/NLpd
CELL PRODUCT QUALITY TESTING AND COLLECTION DEVICE
TECHNICAL FIELD The invention relates to the field of cell product technolo- gy, in particular to a cell product quality testing and collection device
BACKGROUND ART Cell products are prepared by in vitro culture for human dis- ease prevention, treatment and diagnosis. During the cell culture process, contamination by bacteria, fungi and mycoplasma seriously affects the quality of cell products and it is required to test the quality of cell products.
However, when testing the quality of cell products, the staff needs to wear sterile clothing and use pipettes to collect cell products, which collection process is troublesome, cannot conven- iently and quickly collect the cell products, cannot reduce the contact between the staff and the utensils, and cannot further en- sure the sterility of the collection process.
Therefore, we present the cell product quality testing and collection device to solve the above proposed problems.
SUMMARY The purpose of the invention is to provide a cell product quality testing and collection device to solve the problems of neither being able to collect cell products conveniently and quickly in the current market nor being able to reduce the contact between the staff and the utensils, as proposed in the background art above.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: a cell product quality testing and collection device, which comprises a sterile table, inside of whose lower part an motor is mounted and at whose inner side a placement seat is connected in a clamped way; It also comprises the followings:
Main rotating rod, whose lower end is connected to the output end of the said motor and to whose outer side a semi-gear is con- nected through a key, with a main gear engaged to the right of the said semi-gear, a secondary rotating rod connected through a key to the inner side of the said main gear, and a pinion connected through a key to the outer side above the said secondary rotating rod; Supporting ring, which is arranged outside of the said place- ment seat and above which a supporting seat is provided with, with a test tube provided inside of the said supporting seat; Stop collar, which is mounted on the outer side above the said supporting ring, with cluster gear connected to the outer side below the said supporting ring; Mounting frame, which is fixed with bolts inside of the said sterile table, and at whose upper end a collection tube is mount- ed, with a pull rod connected in a sliding manner to the upper side wall of the said collection tube and a piston mounted at the lower end of the said pull rod; Plugging balls, which are arranged below and to the left of the interior of the lower end of the said collection tube, and to the middle of whose outer side a movable rod is connected , with the said movable rod elastically connected to the said collection tube by means of a spring; Through slots, which are arranged on the bottom and the left side below the said collection tube, with the said through slot on the bottom connected to a main needle and that on the left side connected to a secondary needle.
Preferably, the outer diameter of the said semi-gear is twice that of the said main gear, and the number of teeth of the said semi-gear is the same as that of the said main gear, through which design the semi-gear can be used to drive the main gear to rotate intermittently, and it can be ensured that when the semi-gear ro- tates by one cycle the main gear is driven to rotate by one cycle.
Preferably, the said main gear and the said pinion are con- nected in a coaxial manner, and the said main gear and the said pinion have the same outer diameter and number of teeth, which de- sign can ensure the synchronous rotation of the main gear and the pinion.
Preferably, the said cluster gears are distributed at equal angles at the junction of the said supporting ring and the said pinion, and the said supporting ring forms a rotating structure with the said sterile table through the said stop collar, with the number of teeth of the said pinion being one-fifth of the number of the said cluster gears, which design ensures that the pinion drives the supporting ring to rotate and that when the pinion ro- tates by one cycle the supporting ring is driven to rotate by one- fifth cycle.
Preferably, the top section of the said supporting seats is an arc-shaped structure, the said supporting seats are distributed in 5 groups at equal angles about the longitudinal center line of the said supporting ring, and the position of the slit in the said supporting seat is corresponding to that of the said secondary needle, through which design the positions of the support seats can be adjusted and it can be ensured that the support seat on the left corresponds accurately to the secondary needle.
Preferably, the main section of the said through-slot below is of a "U" shaped structure, and the 2 said through-slots are distributed in a mutually perpendicular orientation.
Preferably, the said plugging balls and the said through slots are connected in a clamped way, and the contact surface of the said through slots and the said plugging balls is of a curved structure, through which design the plugging balls can be used to close and open the through slots to achieve the orientation ad- justment of the flow of cell products.
Compared with the prior art, the beneficial effect of the in- vention is as follows: (1) The cell product quality testing and collection device is equipped with a collection tube, the piston controlled to move up- ward and the plugging ball below driven by air pressure to move upward so as to smoothly adsorb the cell product into the collec- tion tube, then the piston driven to move downward and the plug- ging ball at left driven by air pressure to move to the left so as to smoothly collect the cell product into the test tube. This pro- cess only requires the staff to drive the piston move upward and downward, which operation is convenient and avoids direct contact between the staff and the collection tool, more conducive to ster- ile collection. (2) The cell product quality testing and collection device is provided with a supporting ring, which drives the semi-gear to ro- tate, driving the main gear to intermittently rotate and synchro- nously controlling the coaxially connected secondary gear to ro- tate, which controls the supporting ring to rotate by one-fifth cycle through the cluster gears connected through engagement to exchange the positions of the adjacent 2 supporting seats, align- ing the position of the test tube with that of the secondary nee- dle, and facilitating the continuous collection of cell products in batch.
BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic diagram for the main section of the invention; Figure 2 is a schematic diagram for the main section of the supporting ring in the invention; Figure 3 is a schematic diagram for the main section of the plugging ball in the invention; Figure 4 is a schematic diagram for the top section of the invention; Figure 5 is a schematic diagram for the top section of the supporting ring in the invention; Figure 6 is a schematic diagram for the top section of the semi-gear in the invention; Figure 7 is a schematic diagram for the upward-view structure of the collection tube in the invention; Figure 8 is a schematic diagram for the three-dimensional structure of the placement seat in the invention. In the figures: 1. sterile table; 2. motor; 3. main rotating rod; 4. semi-gear; 5. main gear; 6. secondary rotating rod; 7. pinion; 8. placement seat; 9. supporting ring; 10. cluster gear;
11. stop collar; 12. supporting seat; 13. test tube; 14. mounting frame; 15. collection tube; 16. pull rod; 17. piston; 18. movable rod; 19. spring; 20. through slot; 21. main needle; 22. secondary needle; 23. plugging ball.
DETAILED DESCRIPTION OF THE EMBODIMENTS The technical scheme in the embodiment of the invention will 5 be clearly and completely described below in combination with the drawings in the embodiment of the invention. Obviously, the de- scribed embodiment is only a part of the embodiments of the inven- tion and not all of them. Based on the embodiment in the inven- tion, all other embodiments obtained by a person of ordinary skill in the art without making creative work fall within the scope of protection of the invention.
As shown in Figures 1-8, the technical scheme adopted by the invention is as follows: a cell product quality testing and col- lection device, which comprises a sterile table 1, inside of whose lower part an motor 2 is mounted and at whose inner side a place- ment seat 8 is connected in a clamped way; Main rotating rod 3, whose lower end is connected to the out- put end of the motor 2 and to whose outer side a semi-gear 4 is connected through a key, with a main gear 5 engaged to the right of the semi-gear 4, a secondary rotating rod 6 connected through a key to the inner side of the main gear 5, and a pinion 7 connected through a key to the outer side above the secondary rotating rod 6; Supporting ring 9, which is arranged outside of the placement seat 8 and above which a supporting seat 12 is provided, with a test tube 13 provided inside of the supporting seat 12; Stop collar 11, which is mounted on the outer side above the supporting ring 9, with cluster gear 10 connected to the outer side below the supporting ring 9; Plugging balls 23, which are arranged below and to the left of the interior of the lower end of the collection tube 15, and to the middle of whose outer side a movable rod 18 is connected , with the movable rod 18 elastically connected to the collection tube 15 by means of a spring 19; Through slots 20, which are arranged on the bottom and the left side below the collection tube 15, with the through slot 20 on the bottom connected to a main needle 21 and that on the left side connected to a secondary needle 22.
The outer diameter of the semi-gear 4 is twice that of the main gear 5, and the number of teeth of the semi-gear 4 is the same as that of the main gear 5; the main gear 5 and the pinion 7 are connected in a coaxial manner, and the main gear 5 and the pinion 7 have the same outer diameter and number of teeth; the cluster gears 10 are distributed at equal angles at the junction of the supporting ring 9 and the pinion 7, and the supporting ring 9 forms a rotating structure with the sterile table 1 through the stop collar 11, with the number of teeth of the pinion 7 being one-fifth of the number of the cluster gears 10; the top section of the supporting seats 12 is an arc-shaped structure, the sup- porting seats 12 are distributed in 5 groups at equal angles about the longitudinal center line of the supporting ring 9, and the po- sition of the slit in the supporting seat 12 is corresponding to that of the secondary needle 22; the main section of the through- slot 20 below is of a "U" shaped structure, and the 2 said through-slots 20 are distributed in a mutually perpendicular ori- entation; the plugging balls 23 and the through slots 20 are con- nected in a clamped way, and the contact surface of the through slots 20 and the plugging balls 23 is of a curved structure; The cell products can be concentrated in the placement seat 8; as shown in Figures 2-3, pull the pull rod 16 upward to control the piston 17 to move upward, thereby adjusting the air pressure in the collection tube 15 to adsorb and controlling the plugging ball 23 below to move upward; the plugging ball 23 then controls the movable rod 18 to squeeze the spring 19 to move upward to un- block the through slot 20 below by removing the plugging ball 23 so that the cell products enter into the collection tube 15 via the main needle 21 and the through slot 20. At the same time, the motor 2 is powered on and is controlled to slowly rotate, as shown in Figure 6, the motor 2 controls the main rotating rod 3 to drive the semi-gear 4 to rotate, the semi-gear 4 controls the main gear 5 it is connected to through engagement to rotate intermittently, and when the semi-gear 4 rotates by one cycle the main gear 5 is driven to rotate by one cycle; the main gear 5 controls the coaxi- ally connected pinion 7 to rotate, and the pinion 7 controls the supporting ring 9 to rotate by one-fifth cycle through the cluster gear 10 it is connected through engagement.
As shown in Figure 4, the positions of the adjacent 2 supporting seats 12 can be ex- changed to align the position of the test tube 13 with that of the secondary needle 22; push down the pull rod 16 to control the pis- ton 17 to move downward to adjust the air pressure in the collec- tion tube 15 to push the plugging ball 23 below to close the through slot 20 below and the plugging ball 23 on the left side to move left so that the cell products are discharged through the through slot 20 on the left side then enter into the test tube 13 through the main needle 21. Repeat the steps above to realize the collection of cell products; Mounting frame 14, which is fixed with bolts inside of the sterile table 1, and at whose upper end a collection tube 15 is mounted, with a pull rod 16 connected in a sliding manner to the upper side wall of the collection tube 15 and a piston 17 mounted at the lower end of the pull rod 16; The collection tube 4 can be taken off by removing the bolts used for fixing the mounting frame 14, thereby taking off the placement seat 8 connected to the sterile table 1 in a clamped way for easy cleaning and sterilization of the placement seat 8. Working philosophy: When using this cell product quality testing and collection device, firstly, the cell products can be concentrated in the placement seat 8; pull the pull rod 16 upward so that the cell products enter into the collection tube 15 via the main needle 21 and the through slot 20. At the same time, the motor 2 is powered on, the positions of the adjacent two support- ing seats 12 are exchanged to align the position of the test tube 13 with that of the secondary needle 22; push down the pull rod 16 so that the cell products are discharged through the through slot 20 on the left side then enter into the test tube 13 through the main needle 21 to realize the collection of cell products.
What is not described in detail in this specification belongs to the prior art that is well known to the persons of professional skill in the art In the description of the invention, it should be noted that unless otherwise expressly specified and limited, the term "con-
nection” should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integral connection; it may be a mechanical connection, or an electrical connection; it may be a direct connection, or an indirect connec- tion through an intermediary.
To a person with ordinary skill in the art, the specific meaning of the above terms in the invention can be understood on a case-by-case basis.
Despite the detailed description of the invention with refer- ence to the preceding embodiment, it is still possible for a per- son skilled in the art to modify the technical schemes recorded in the preceding embodiment or to make equivalent substitutions for some of the technical features; any modifications, equivalent sub- stitutions, improvements, etc. made within the scope of spirit and principles of the invention should be included in the scope of protection of the invention.

Claims (2)

CONCLUSIESCONCLUSIONS 1. Apparaat voor het testen van de kwaliteit en het verzamelen van celproducten, omvattende een steriele tafel, waarbij aan de bin- nenkant van het onderste deel een motor is gemonteerd en waarbij aan de binnenkant ervan op klemmende wijze een plaatsingszitting is verbonden; waarbij het apparaat wordt gekenmerkt doordat het ook het volgende omvat: een roterende hoofdstang, waarvan het onderste uiteinde is verbon- den met het uitgangseinde van de genoemde motor en waarbij aan de buitenzijde ervan een semi-tandwiel is verbonden via een spie, met een hoofdtandwiel ingeschakeld rechts van het genoemde semi- tandwiel, een secundaire roterende staaf die via een spie is ver- bonden met de binnenkant van het hoofdtandwiel, en een rondsel dat via een spie is verbonden met de buitenkant boven de secundaire roterende staaf; een steunring, die buiten de genoemde plaatsingszitting is aange- bracht en waarboven een steunzitting is voorzien, met een reageer- buis die binnen de genoemde steunzitting is aangebracht; een aanslagkraag, die aan de buitenzijde boven de genoemde steun- ring is gemonteerd, met een clustertandwiel verbonden met de bui- tenzijde onder de genoemde steunring; een montageframe, dat met bouten aan de binnenkant van de genoemde steriele tafel is bevestigd, en waarbij aan het boveneinde ervan een verzamelbuis is gemonteerd, met een trekstang die op een glij- dende manier is verbonden met de bovenste zijwand van de genoemde verzamelbuis en waarbij een zuiger is gemonteerd op het onderste uiteinde van de genoemde trekstang; afsluitkogels, die zijn aangebracht onder en links van het inwen- dige van het onderste uiteinde van de verzamelbuis, en waarbij aan het midden van de buitenzijde ervan een beweegbare staaf is ver- bonden, waarbij de beweegbare staaf elastisch is verbonden met de verzamelbuis door middel van een veer; doorgaande sleuven, die aan de onderzijde en de linkerzijde onder de genoemde opvangbuis zijn aangebracht, waarbij de genoemde door-An apparatus for quality testing and collection of cellular products comprising a sterile table, wherein a motor is mounted on the inside of the lower part and a locating seat is clamped to the inside thereof; the device being characterized in that it also comprises: a rotating main rod, the lower end of which is connected to the output end of said motor and in which on the outside thereof a semi-gear is connected via a key, to a main gear engaged to the right of said semi-gear, a secondary rotary rod keyed to the inside of the main gear, and a pinion gear keyed to the outside above the secondary rotary rod; a support ring disposed outside said locating seat and above which a support seat is provided with a test tube disposed within said support seat; a stop collar mounted externally above said support ring, with a cluster gear connected to the outside below said support ring; a mounting frame, bolted to the inside of said sterile table, and having a collecting tube mounted at its upper end, having a tie rod slidably connected to the upper side wall of said collecting tube and having a piston is mounted on the lower end of said tie rod; sealing balls mounted below and to the left of the interior of the lower end of the collecting tube, and having a movable rod connected to the center of the outside thereof, the movable rod being elastically connected to the collecting tube by means of from a spring; through-slits provided at the bottom and the left side below said receiving tube, said through-slits gaande sleuf aan de onderzijde is verbonden met een hoofdnaald en die aan de linkerzijde is verbonden met een secundaire naald.going slot on the bottom is connected to a main needle and the one on the left is connected to a secondary needle. 2. Apparaat voor het testen van de kwaliteit en het verzamelen van celproducten, zoals beschreven in conclusie 1, waar bij het appa- raat wordt gekenmerkt doordat het hoofdtandwiel en het rondsel op coaxiale wijze zijn verbonden, en het hoofdtandwiel en het rondsel dezelfde buitendiameter en hetzelfde aantal tanden hebben.An apparatus for quality testing and collection of cell products as set forth in claim 1, wherein the apparatus is characterized in that the main gear and the pinion are coaxially connected, and the main gear and the pinion have the same outer diameter and have the same number of teeth.
NL2030710A 2022-01-25 2022-01-25 Cell product quality testing and collection device NL2030710B1 (en)

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NL2030710A NL2030710B1 (en) 2022-01-25 2022-01-25 Cell product quality testing and collection device

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Application Number Priority Date Filing Date Title
NL2030710A NL2030710B1 (en) 2022-01-25 2022-01-25 Cell product quality testing and collection device

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NL2030710A true NL2030710A (en) 2022-07-08
NL2030710B1 NL2030710B1 (en) 2022-11-15

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314825A (en) * 1992-07-16 1994-05-24 Schiapparelli Biosystems, Inc. Chemical analyzer
WO2009026060A2 (en) * 2007-08-20 2009-02-26 Mallinckrodt Inc. Fluid driven medical injectors
CN108660077A (en) * 2018-06-20 2018-10-16 新乡医学院 A kind of tumour cell heating device sampled automatically
CN111304056A (en) * 2020-03-11 2020-06-19 李容尔 Cell sample draws partial shipment device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5314825A (en) * 1992-07-16 1994-05-24 Schiapparelli Biosystems, Inc. Chemical analyzer
WO2009026060A2 (en) * 2007-08-20 2009-02-26 Mallinckrodt Inc. Fluid driven medical injectors
CN108660077A (en) * 2018-06-20 2018-10-16 新乡医学院 A kind of tumour cell heating device sampled automatically
CN111304056A (en) * 2020-03-11 2020-06-19 李容尔 Cell sample draws partial shipment device

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