MY201633A - Kit and method for multiplex detection of chikungunya virus and zika virus - Google Patents
Kit and method for multiplex detection of chikungunya virus and zika virusInfo
- Publication number
- MY201633A MY201633A MYPI2020003103A MYPI2020003103A MY201633A MY 201633 A MY201633 A MY 201633A MY PI2020003103 A MYPI2020003103 A MY PI2020003103A MY PI2020003103 A MYPI2020003103 A MY PI2020003103A MY 201633 A MY201633 A MY 201633A
- Authority
- MY
- Malaysia
- Prior art keywords
- seq
- primer
- kit
- virus
- chikungunya
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A kit for multiplex detection of Chikungunya virus and Zika virus includes a first primer set specific to Chikungunya and a second primer set specific to Zika. The first primer set includes a forward primer with SEQ ID NO: 1 and a reverse primer with SEQ ID NO: 2. The second primer set includes a first forward primer with SEQ ID NO: 4, a second forward primer with SEQ ID NO: 5, and a reverse primer with SEQ ID NO: 6. The kit also includes a probe specific to Chikungunya with SEQ ID NO: 3 and a probe specific to Zika with SEQ ID NO: 7. The kit further includes an internal control template, which is in vitro transcribed RNA of proA gene from Pectobacterium carotovorum, a forward primer with SEQ ID NO: 8, a reverse primer with SEQ ID NO: 9, and a probe with SEQ ID NO: 10
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SG10201905639PA SG10201905639PA (en) | 2019-06-19 | 2019-06-19 | Primer pair, kit and method for detecting chikungunya virus |
| SG10201905641YA SG10201905641YA (en) | 2019-06-19 | 2019-06-19 | Primer pair, kit and method for detecting zika virus |
| SG10202005575VA SG10202005575VA (en) | 2019-06-19 | 2020-06-12 | Kit and method for multiplex detection of chikungunya virus and zika virus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MY201633A true MY201633A (en) | 2024-03-06 |
Family
ID=73799512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MYPI2020003103A MY201633A (en) | 2019-06-19 | 2020-06-17 | Kit and method for multiplex detection of chikungunya virus and zika virus |
Country Status (3)
| Country | Link |
|---|---|
| CN (1) | CN112111599A (en) |
| MY (1) | MY201633A (en) |
| TW (1) | TWI754967B (en) |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935715B (en) * | 2010-09-08 | 2012-02-22 | 中国检验检疫科学研究院 | Method for detection of chikungunya virus nucleic acid by real-time fluorescent quantitative PCR |
| WO2014039010A1 (en) * | 2012-09-04 | 2014-03-13 | Republic Polytechnic | Isolated oligonucleotides, methods and kits for detection, identification and/or quantitation of chikungunya and dengue viruses |
| US11591660B2 (en) * | 2016-01-14 | 2023-02-28 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and reagents for detection of chikungunya virus or chikungunya virus and dengue virus |
| US10655190B2 (en) * | 2016-03-11 | 2020-05-19 | Roche Molecular Systems, Inc. | Compositions and methods for detection of Zika virus |
| CN105838713A (en) * | 2016-05-20 | 2016-08-10 | 深圳市第三人民医院 | Method for detecting fluorogenic quantitative PCR of Zika virus, primers and kit |
| CN106086242A (en) * | 2016-07-29 | 2016-11-09 | 广州市第八人民医院 | A kind of test kit detected for Flavivirus fast typing and virus load |
| CN107723382A (en) * | 2016-08-09 | 2018-02-23 | 博奥生物集团有限公司 | Hereby block LAMP primer composition and its application of virus for detecting |
| CN106244726A (en) * | 2016-08-19 | 2016-12-21 | 中检国研(北京)科技有限公司 | A kind of zika virus loop-mediated isothermal amplification detection kit and using method |
| CN106521032B (en) * | 2016-11-30 | 2019-09-06 | 博奥生物集团有限公司 | A nucleic acid amplification technique and its application in mosquito-borne virus detection |
| CN106755571B (en) * | 2016-11-30 | 2020-08-07 | 博奥生物集团有限公司 | A kit for the detection of Zika, Dengue and Chikungunya viruses |
| CN106755573A (en) * | 2016-12-07 | 2017-05-31 | 深圳澳东检验检测科技有限公司 | Zika virus, dengue fever virus, the RT PCR detection methods of chikungunya fever virus, primer and probe and kit |
| CN108330210B (en) * | 2017-01-18 | 2021-07-30 | 广州市疾病预防控制中心 | Zika virus, dengue virus and chikungunya virus nucleic acid detection kit and application thereof |
| WO2018169550A1 (en) * | 2017-03-17 | 2018-09-20 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Real-time rt-pcr assay for detection of dengue, chikungunya, and zika viruses |
| KR20190002245A (en) * | 2017-06-29 | 2019-01-08 | 조용직 | Dual diagnostic kit and diagnostic method for zika virus |
| CN109423526A (en) * | 2017-08-29 | 2019-03-05 | 湖北省疾病预防控制中心 | Mosquito matchmaker's infectious disease pathogens premix fluorescence PCR detection reagent and kit |
-
2020
- 2020-06-17 TW TW109120487A patent/TWI754967B/en active
- 2020-06-17 MY MYPI2020003103A patent/MY201633A/en unknown
- 2020-06-18 CN CN202010559238.3A patent/CN112111599A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| TWI754967B (en) | 2022-02-11 |
| CN112111599A (en) | 2020-12-22 |
| TW202115262A (en) | 2021-04-16 |
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