MY137744A - Herbicide resistant plants - Google Patents

Abstract

THE PRESENT INVENTION PROVIDES, INTER ALIA, A GLYPHOSATE RESISTANT EPSPS ENZYME WHEREIN IN COMPARISON WITH THE WILD TYPE ENZYME THE EPSPS PROTEIN SEQUENCE IS MODIFIED IN THAT A FIRST POSITION IS MUTATED SO THAT THE RESIDUE AT THIS POSITION IS ILE RATHER THAN THR AND A SECOND POSITION IS MUTATED SO THAT THE RESIDUE AT THIS POSITION IS SER RATHER THAN PRO, THE MUTATIONS BEING INTRODUCED INTO EPSPS SEQUENCES WHICH COMPRISE THE CONSERVED REGION GNAGTAMRPL IN THE WILD TYPE ENZYME SUCH THAT MODIFIED SEQUENCE READS GNAGIAMRSL, WHEREIN THE ENZYME DOES NOT COMPRISE THE SEQUENCE K(AN)AKRA VVVGCGGKFPVE CHARACTERISED IN THAT THE ENZYME IS STABLE AND POSSESSES AT LEAST ONE OF THE FOLLOWING SEQUENCE MOTIFS (I) TO (VIII) IN WHICH X IS ANY AMINO ACID AND Z, Z1 AND Z2 ARE ANY AMINO ACID OTHER THAN THOSE SPECIFIED:- (i)ALLMZAPLA, WHEREIN Z IS NOT S OR T; (ii)EIEIZDKL, WHEREIN Z IS NOT V; (iii)FG(V /I)(K/S)ZEH, WHEREIN Z IS NOT V; (iv)AL(K/R)ZLGL, WHEREIN Z IS NOT R; (v)GLXVEZ1DXZ2XXXA(I/N)V, WHEREIN Z1 IS NOT T AND/OR Z2 IS NOT E; (vi)ITPPZ1K(LN)(K/N)Z2, WHEREIN Z1 IS NOT K AND/OR Z2 IS NOT T; (vii)TIZ(D/N)PGCT, WHEREIN Z IS NOT N OR L; (viii)(D/N)YFXVLXZXX(K/R)H, WHEREIN Z IS NOT R. THE INVENTION ALSO INCLUDES GLYPHOSATE RESISTANT PLANTS REGENERATED FROM MATERIAL TRANSFORMED WITH POLYNUCLEOTIDES WHICH ENCODE SUCH EPSPS ENZYMES AND OPTIONALLY ENZYMES (SUCH AS PHOSPHOENOL PYRUVATE SYNTHASE AND PYRUVATE ORTHOPHOSPHATE DI-KINASE) WHICH PROVIDE FOR ELEVATED CHLOROPLASTIC LEVELS OF PHOSPHOENOL PYRUVATE, AND A METHOD OF SELECTIVELY CONTROLLING WEEDS IN A FIELD COMPRISING SUCH PLANTS AND GLYPHOSATE SENSITIVE WEEDS, BY THE APPLICATION TO THE FIELD OF GLYPHOSATE OR AN AGRONOMICALLY ACCEPTABLE DERIVATIVE.