MXPA99001828A - Lip treatment containing live yeast cell derivative - Google Patents

Abstract

A lip treatment containing a bioyeast ingredient, such as a live yeast cell derivative, and a suitable carrier. The live yeast cell derivative is produced by lysing and concentrating the cell contents of yeast cells subjected to an extended period of non-lethal stress such as is induced by exposure to certain wavelengths of ultraviolet light. The lip treatment rapidly moisturizes, smoothes, and heals the lips, and provides for rapid exfoliation of chapped cells on the lips. Thus, the treated lips are restored to a soft and supple condition.

Description

LIPS TREATMENT CONTAINING A LIVE YELLOW CELL DERIVATIVE The present invention relates in general to cosmetic formulations containing a biofuel derivative, which are suitable to be applied to the lips. More preferably, the present invention relates to a lipstick or ointment having a living yeast cell derivative that acts as a moisturizing, healing and softening agent. BACKGROUND OF THE INVENTION The skin of the lips is structurally different from the skin of other parts of the human body.

The lips have a thinner stratum corneum and a smaller amount of lipids than the skin that is not of the lips or typical skin. This allows the water to pass easily and dissipate from the lips. In fact, the loss of water through the lips is 10 times greater than the loss of water through the typical skin. This makes the lips more prone to drying out and, over time, to more damage. The stratum corneum of the lips also has a high index of turnover, which means that the corneocytes are constantly occurring. As part of the production process, the dead cells are REF. 29562 clustered in flakes on the surface of the lips. When the lips are dry, these flakes are more evident. In addition, the lips have a lower number of melanocytes or melanin-producing cells, compared to typical skin. In the absence of large numbers of these melanocytes, the lips have less melanin and are more prone to receive damage induced by the sun's UV rays. Consistent with this, consumers demand an effective treatment for the lips that moisturizes, heals and softens the vulnerable and delicate surface thereof. Most lip products on the market consist of occlusive oils or waxes, which limit the amount of moisture that escapes from the lips. These products do not treat any underlying dryness and are only to prevent further drying. Some products also have moisturizing and healing ingredients. The effectiveness of these products varies widely. Derivatives from washing cells are known in the art for the general treatment of the skin. U.S. Patent No. 2,320,478 to Sperti describes the derivative of living yeast cells which is a preferred component of the present invention, and describes its general topical application in lotions, creams and oils. The patent discloses that the derivative of living yeast cells improves respiration of the skin [column 3, rows 10.18]. However, improved normal labial tissue respiration presumably would lead to greater moisture loss and, therefore, this derivative would not be beneficial for the labial tissue. Because the lips are so vulnerable to moisture loss, any likely increase in the rate of moisture loss would be very damaging to a cosmetic product and would tend to offset any other benefit provided by the product. Surprisingly, it has been found that this derivative of live yeast cells, when incorporated into lip preparations, such co or treatments for the lips, lip balms, lipstick or ointments, has a drastic moisturizing, healing and softening effect on the lips , especially on dry lips. The live yeast cell derivative is produced by using and concentrating the cellular content of yeast cells subjected to an extended period of non-lethal stress, such as can be induced by exposing them to certain wavelengths of ultraviolet light. Applicants hereby have a pending US Patent Application No. 8 / 602,004, filed on February 15, 1996. The pending application relates to a composition containing a derivative of living yeast cells in combination with other active ingredients such as ascorbyl magnesium phosphate, tocopherol acetate and retinol palmitate, in a vehicle, for the treatment of discoloration of the skin under the eyes. BRIEF DESCRIPTION OF THE INVENTION Against the foregoing background, a primary objective of the present invention is to provide a lip preparation containing an effective amount of a derivative of living yeast cells. Another objective of the present invention is to provide a lip preparation that has moisturizing, healing and softening properties for the lips. Yet another object of the present invention is to provide a lip preparation that causes a rapid exfoliation of the split lip cells and that restores a smooth texture to the lips. A further object of the present invention is to provide a derivative of living yeast cells which is compatible with cosmetic lipsticks, such that it is suitable for use in treatments for the lips such as lipstick, lip balm and ointments. To achieve the above objects and advantages, the present invention, in brief, comprises a derivative of living yeast cells in a cosmetic vehicle suitable for being applied to the lips. The present invention further comprises a composition containing a derivative of living yeast cells derived from cells that have been subjected for a time to non-lethal trauma. This induces the yeast cells to produce unique curative compounds that are contained in the resulting yeast cell derivative. The composition further comprises a suitable vehicle to be applied to the lips. DESCRIPTION OF THE PREFERRED MODALITIES The yeast cell derivative of the present invention is described substantially in US Pat. No. 2,320,478 to Sperti, described above. The patent is incorporated herein by reference. The present invention includes topical compositions containing a derivative of living yeast cells (hereinafter referred to as "DCLV") as an ingredient in a suitable vehicle, to be applied to the lips. The DCLV is a highly soluble material. This DCLV is preferably produced in accordance with the following method. Yeast cultures are placed in a fermentor. These cultures are brought to viability by using the appropriate nutrient media with good aeration under controlled temperature conditions. The live yeast cells are subsequently subjected to a "damage" process such as irradiating them with UV light of a specific wavelength (286 nm). The cells respond to the effects of UV light by producing several protective substances. The process of "damage" is continued until the complex biochemical protection mechanisms are complete (this takes several days). The fermentation is stopped by breaking the cell wall of the yeasts using a proteolytic enzyme. The insoluble cell wall material is removed by centrifugation, leaving the cell protoplasm. Then, the protoplasmic derivative is concentrated and the biological activity is evaluated. The DCLV derivative can be concentrated by lyophilization or by spray dehydration. It is commercially available as a hygroscopic fine powder or as a filtered solution. The preferred form is a refined powder. Then, the powder or DCLV solution can be incorporated into several suitable vehicles to be applied to the lips. The DCLV is preferably present in an amount of about 0.01 to 15.00% by weight with respect to the total weight of the lipstick or other composition. At concentrations greater than 15%, the cost of DCLV will become prohibitive. In a preferred embodiment, the base is from about 70.00% to about 90.00% by weight with respect to the total weight of the composition, the DCLV is from about 0.01 to about 15.00% by weight, the dyes are about 15.00% by weight, fragrances up to about 3.00% by weight and other ingredients such as skin protectors, they are from about 0.01 to about 10.0% by weight. The skin protectants should include allantoin in an amount of about 0.50 to about 2.0% by weight. Another preferred lip composition according to the present invention includes a derivative of living yeast cells and a suitable carrier or base, wherein the derivative of living yeast cells is a derivative of used saccharomyces. Preferably, this preferred embodiment of the labial product comprises from about 0.01 to about 15% of the derivative of living yeast cells. The vehicle or base preferably comprises from about 60 to about 90% and more preferably 76% by weight, based on the total weight of the lip composition, and humectants, which may be part or most of the vehicle, may comprise approximately same amount. Preferred humectants include lanolin, vegetable oil, risino oil, isopropyl palmitate, mineral oil, avocado oil petrolatum, soybean oil, caprylic / capric triglycerides, diisopropyl dimerate, methicone or a combination thereof. A preferred lip composition of the present invention also includes about 0.60% curative. Preferred curatives include vitamin E, allantoin, candelilla wax, aloe vera, petrolatum, calamine, dimethicone, cocoa butter, shark liver oil, glycerin, zinc oxide, aluminum hydroxide, kaolin, zinc acetate, carbonate of zinc or a combination thereof. A preferred lip composition may also include about 15.0% of an occlusive wax. Preferred waxes include candelilla wax, ceresin wax, ozokerite, lanolin wax, beeswax, paraffin wax, microcrystalline wax, animal wax, vegetable wax, syncretic wax, mineral wax, Japan wax, whale sperm, sunflower wax , orange wax, shelac wax, rice wax, jojoba wax, rose wax, jasmine wax or a combination thereof. Additionally, certain preferred lip compositions may include an additional dye or active ingredients. The preferred active ingredients are allantoin, aloe vera, elastin, collagen, vitamin E and derivatives thereof, vitamin A and derivatives thereof, liposomes, sodium hyaluronate, water, botanical extracts, phospholipids, silk powder, primrose oil ,, cholesteryl glutamate, hyaluronic acid, lauroyl lysine or a combination thereof. A preferred lip composition according to the present invention includes from about 70 to about 90% by weight of a base, including hydrolyzed lanolin and polybutene, and from about 0.01"to about 15% by weight of a derivative of living yeast cells. Optimally, the DCLV is present in an amount of about 0.5% by weight.

Preferably, this composition also includes from about 2.0 to about 9.0% by weight of colorants, about 76% by weight of the base. The following is a preferred illustrative embodiment, wherein all percentages are expressed in relation to the weight of the ingredient with respect to the total weight of the composition (this applies to Examples 2-7 as well). EXAMPLE 1 Treatment in Lipstick% in weight DCLV 0.50 Base Approx. 76.45 May include the following: Ozoquerite 170-D 5.50 Linear Polyethylene PL 3.00 White Microcera 5.00 Low Odor Lanolin 12.00 Diglyceryl Diisostearate 11.50 Isobutyrate Sucrose Acetate 10.00 Isopropyl Isobutyrate 5.00 Silica S-700 - Japan 2.00 Propylparaben 0.20 Diisopropyl Diimerate 22.25 Total Colorants Approx. 13.00 It may include the following: Pigment from 4.00 to 12.00 Barium Sulfate from 0.00 to 3.50 Pearl from 0.00 to 7.00 Fine mica from 1.00 to 4.00 Other Ingredients Approx. 9,925 It may include the following: Allantoin 0.60 Other skin protectors 9.325 Fragrance Approx. 0.125 The above base ingredients are only representative of what those skilled in the art would include as a base ingredient in a lipstick treatment. The lipstick of Example 1 was evaluated, under blind conditions, to test the overall acceptability, aesthetics and performance of this embodiment of the present invention. A test of two weeks of use in the consumer's home was conducted. Fifty-six participants were surveyed after seven and fourteen days of use. All the participants were women, with an age of 25 to 55 years and who said they had dry lips or broken lips, but not having them cracked or bleeding. The following table summarizes the results of the survey.

The results are expressed as a percentage of respondents.

Where there are entries that were left blank, the respondents were not tested on that topic on that date. Test topic 7 days 14 days Correct amount of color deposited 74% Color very / moderately even 95 Greasy / non-greasy 91 Acceptable 100 Aspect of the application very / moderately attractive 73 Product very / moderately visible. 90 Taste not good or bad 73 Very / moderately soft 90 93 Very / moderately effective as a humectant 80 75 It lasts much longer / a little longer than expected 66 62 Very / moderately acceptable use 16 79 Repaired his lips - in accordance 78 77 It gives the lips a fuller appearance - in agreement 62 64 Retexturizes the lips to their most natural form - according 56 55 Makes lips feel flexible - agree 79 84 Makes the lips look younger - agree 53 58 Minimizes the appearance of lines / wrinkles on the lips - in agreement 60 64 Makes the lips look smoother -according 75 80 It is ultracremous - in agreement 90 89 Shine in the smooth - agree 100 96 Prevents lips from drying out - in accordance 84 Prevents the lips from peeling - in agreement 74 76 Provides an exuberant extraordinary color - according 74 73 Redefine your lips naturally - agree 64 64 Makes the lips look thicker - agree 52 57 Makes lips feel more flexible 78 82 It is not light - agree 55 71 Provides full coverage - agree 93 91 It is of prolonged use - in accordance 83 82 Improves the appearance of the lips - in accordance 79 82 These data support the conclusion that respondents observed a significant improvement in the appearance of their lips at both 7 and 14 days of use. With continuous use, the product of Example 1 effectively corrected flayed, cracked and parched lips that bothered these respondents. Additional tests were performed to determine the ability of the preferred embodiment of Example 1 to increase the moisture content and prevent moisture loss when applied to the lips. Example 1, as already described, includes DCLV, occlusive waxes and humectant ingredients such as cholesteryl glutamate and aloe vera gel.

One hour Two hours Moisture content + 432% + 305% Decreases water loss 31% 28% The study was conducted in a panel of 15 women aged 25 to 45 years. The women were instructed not to moisten their lips during the duration of the studies. After a period of 30 minutes of acclimatization, a tongue repressor was inserted into the mouth of each panelist as a physical barrier against unintentional wetting of the lips. After 5 minutes, the baseline values were measured. Once the baseline measurements were made, the panelists were given the product of Example 1 in a normal manner. Measurements were made after one hour and after two hours, in the lower lip of each panelist. The moisture content of the lips was measured using the Skicon-200 conductance meter, which measures the conductance of the skin, which is a reflection of the water content. The measurements were taken at the baseline (after acclimatization and before lipstick application.) The participants had Skicon labial values of approximately 50. Measurements were also taken after one and two hours of lipstick application. A series of 5 readings was made at each site during each measurement and the average value was calculated.The highest values indicate an increase in the level of hydration and humidification.The average measurements obtained were the following: Baseline 58.4 One hour 310.5 Two hours 236.5 As summarized above, this reflects an increase in wetting of 432% after one hour and 305% after two hours. Water loss or lip barrier function was measured using Evaporí etro Servomed. The loss of transepidermal water (PTEA) is a reaction of the barrier function of the stratum corneum of the lips. A defective barrier results in high values of water loss. Products with occlusive agents decrease the loss of water and provide a barrier. Therefore, low PTEA values after the application of the lipstick indicate the prevention of water loss due to the occlusivity of the product. A third study was conducted to determine the clinical efficacy of the product of Example 1 and its ability to repair dry and damaged lips. The test was performed on fifteen women from 18 to 55 years of age, with dry lips who normally use lip balm, in the cold and dry environment of Innipeg, Canada. Participants were instructed not to use any lip treatment product for three days before starting, testing and using the product of Example 1 for two weeks, in the same way that they use their regular lipstick . Each participant was given a colorful lipstick and a transparent lipstick. The subjects applied the color of their choice or transparent lipstick at least every two hours and after each meal. They were also asked not to use any other lip products during the entire study. The subjects were instructed to apply only clear lipstick on the day their lips were to be evaluated, two hours or more before the visual evaluation, to facilitate the examination of the lips. The subjects were also instructed to avoid contact with water during that period. The lips were evaluated visually by an expert on days 0, 3, 7, 10 and 14 of the treatment. High quality photography was used to graduate and record the lips of the participants, during the course of the study. The photograph showed an obvious decrease in dryness and desquamation during treatment. The lips looked wet, smooth, full and thick after 7 and 14 days of treatment. In addition to an obvious decrease in desquamation, there appears to be an increase in cross-hatched patterns of small lines or padded pattern after wetting. The appearance of the quilted pattern indicates the normalization of the labial texture to a normal and healthy condition.

Days Percent of Percentage of Subjects Improvement 3 93 33 7 100 37 10 100 34 14 93 54 Thus, three days after starting treatment, these formulations improved dryness in 93% of the subjects. The improvement continued during 14 days, observing the greatest reduction of dryness on day 14 (54% with respect to the baseline). The DCLV component can be incorporated into all acceptable lip products vehicles, such as gloss jars, lip gloss pencils, lip balm or ointment, lip conditioners or other lipsticks, with varying levels of dyes. The amount of the DCLV component in a gloss jar of preference is from about 0.50 to about 15.0% by weight with respect to the total weight of the composition. The amount of base, preferably, can vary from about 40 to about 90% by weight. The following are Examples of various compositions of lip products and vehicles which may include the DCLV component. EXAMPLE 2 Jar of Glitter% by weight DCLV 1.00 Base Can include the following: Candelilla Wax 2.0 Ceresin Wax 1670 2.0 Hydroxylated Lanolin from 12.0 to 27.0 Ozoquerite 3.0 Benzoic acid 0.2 Lanolin wax PPG-5 8.0 Soybean oil maleate (UNF) 2.0 Propylene glycol Ricinoleate 2.7 Polybutene 25.0 Capryl / Capric triglycerides 13.3 Ammonium glycyrrhizinate 0.35 Risino oil c.b.p. 100.0 Lecithin 1.3 The composition preferably may include the following additional ingredients in the following preferred amounts: from about 0.10 to about 10.0% dyes and from about 10 to 30% other non-basic ingredients, such as powders, vitamins and fragrances, flavors, oils, waxes, esters, preservatives and water. The dyes may include pigments and barium sulfate (from about 1.2 to about 5.0%) and pearls (up to about 7.0%). Other ingredients may include ethylhexyl methoxycinnamate, benzophenone or other sunscreens (about 1.00% or more). Vitamins can include vitamin E (approximately 0.05%), vitamin C, beta-carotene and vitamin A palmitate 1.65x10 (approximately 0.01%). Approximately 0.1% fragrance or flavoring may be included. As in all examples, trace amounts of other ingredients can also be included. EXAMPLES 3 and 4 Lip Ointment In Lip Ointments, the DCLV is preferably from about 0.30 to about 1.50% by weight with respect to the total weight of the composition and is preferably 1.00% by weight. The base of preference constitutes from about 60.0 to 95.0% and may include the following:% by weight Ex. 3 Ex. 4 Castor oil - Preserved from 39,310 to 44,780 to 42,960 44,960 Delearated lanolin 4,400 4,400 Isopropyl Palmitate 3,300 14,300 Bee Wax 10,400 10,400 Ozokerite 170-D 2,700 2,700 Fatty acid triglycerides 9,600 9,600 Candelilla wax 7,200 7,200 Cetyl 3,300 alcohol 3,300 Butylated hydroxyanisole or 0.010 0.010 Butyl hydroxytoluene Ammonium glycyrrhizinate 0.350 to 0.150 to 1,000 0.350 Benzoic acid 0.200 0.200 Japan toilet 0.500 menthol The composition may also include the following ingredients in the following preferred ranges: colorants such as pigments (from about 0.05 to about 15%); other ingredients such as ethylhexyl methoxycinnamate (about 3.00%) or other sunscreens such as benzophenone; and fragrance / flavor (from about 1.50 to about 3.00%).

EXAMPLE 5 Volatile Base Lipstick In a Volatile Base Lipstick, the DCLV preferably constitutes from about 0.30 to about 5.00% and the base preferably constitutes from about 60 to about 90% of this volatile base lipstick composition. % in ceso DCLV 1.00 Base May include the following: Polyethylene 7.00 Ozoquerite 4.25 to 6.25 Diisostearyl Maleate 8.50 Isobutyrate Sucrose Acetate 13.00 Microcrystalline Wax 1.00 Stearyl Alcohol 0.001 Ethylhexyl Palmitate 2.00 Allantoin 0.20 Vitamin E 0.05 Cyclomethicone -Tetramer 35.00 Nylon powder -espherical 3.00 Diisopropyl dimerate from 6.9969 'to 8.9969 The DCLV can be in the range of about 0.50 to about 2.0% by weight. The dyes, approximately 16%, may include, for example, Red D &C # 21 (0.30%), pigments / pearl (16.70%) and extenders (from 0.00 to 0.0001%). EXAMPLE 6 Lip Conditioner In Lip Conditioners, the DCLV preferably constitutes from about 0.10% to about 2.00% and the base preferably constitutes from about 70 to about 95% of this lip conditioner formulation. % in weigh DCLV 1.00 Base May include the following: Demineralized water 58.94 AEDT Tetrasodium 0.10 Glycerin 5.00 Xanthan Gum 0.10 Grape Seed Oil 5.00 Sunflower Seed Oil 5.00 Vegetable Oil Triglycerides 3.00 Squalene 2.00 Soybean Esterols 1.00 POE Stearate (40M) 2.00 Miristyl Myristate 2.00 Atilic Alcohol 2.00 Glyceryl monostearate 2.50 Yodopropinylbutyl carbamate 0.30 Cyclomethicone 3.00 4.50 Vitamin A / C / E / Beta-0.02 Liposomes Carocenc Benzyl Alcohol 1.00 Benzophenone 3 1.00 Aloe Vera Gel Powder 0.01 Wild Chamomile 0.03 2-phenoxyethanol - 0.50 EXAMPLE 7 Intensive Lip Balm In Intensive Lip Balms, the base preferably constitutes from about 75 to about 95% and the DCLV preferably constitutes from about 0.10 to about 10.00% of the following intensive lip balm formulation. The fragrance preferably constitutes up to about 5.00% of this formulation. % in weigh DCLV ° • 50 Petrolatum Base with Little Flavor 84.95 Benzyl Alcohol 0.50 Ethylhexyl Methoxycinnamate 7.50 Benzophenone 3 3.00 Paraffin Wax 1.00 Butylated hydroxytoluene or 0.50 Hydroxyanisole butylated Triglycerides of Vegetable Oil 2.00 Fragrance 0.50 Thus, the present invention has been described with particular reference to the preferred embodiments thereof, it being obvious that it is possible to make several changes and modifications thereof without departing from the spirit and scope of the invention, as defined in the claims annexes. It is noted that in relation to this date, the best method known by the applicant to carry out the aforementioned invention, is the conventional one for the manufacture of the objects to which it relates.

Claims (22)

1. CLAIMS Having described the invention as above it is claimed as property in the following: 1. A lip composition characterized in that it comprises protoplasm of yeast cells, which is substantially free of yeast cell wall material, and a suitable vehicle.
2. 2. The lip composition according to claim 1, characterized in that the yeast cell protoplasm is a derivative of a used saccharomyces.
3. 3. A lip product characterized in that it comprises from about 0.01 to about 15% of yeast cell protoplasm.
4. 4. A lip composition characterized in that it comprises a yeast cell protoplasm and a suitable vehicle, which, after being applied to the lips of a human being, provides healing, softening and moisturizing properties to the lips.
5. 5. A lip composition characterized in that it comprises approximately 76% by weight with respect to the total weight of the lip composition, of a vehicle and from about 0.01 to about 15% of a yeast cell protoplasmic concentrate.
6. 6. The lip composition according to claim 5, characterized in that it also comprises approximately 75% of a humectant.
7. 7. The lip composition according to claim 6, characterized in that the humectant is selected from the group consisting of lanolin, vegetable oil, risino oil, isopropyl palmitate, mineral oil, petrolatum, avocado oil, soybean oil, caprylic glyceride / capric, diisopropyl dimerate, methicone or a combination thereof.
8. 8. The lip composition according to claim 5, characterized in that it also comprises approximately 0.60% of a healing agent. The lip composition according to claim 8, characterized in that the curative agent is selected from the group consisting of vitamin E, allantoin, candelilla wax, aloe vera, petrolatum, calamine, dimeric, cocoa butter, liver oil, shark, glycerin, zinc oxide, aluminum hydroxide, kaolin, zinc acetate, zinc carbonate or a combination thereof. 10. The lip composition in accordance with. Claim 5, characterized in that it also comprises approximately 15.0% of an occlusive wax. The lip composition according to claim 10, characterized in that the occlusive wax is selected from the group consisting of candelilla wax, ceresin wax, ozokerite, lanolin wax, beeswax, paraffin, microcrystalline wax, animal wax , beeswax, paraffin wax, microcrystalline wax, animal wax, vegetable wax, synthetic wax, mineral wax, Japan wax, whale sperm, sunflower wax, orange wax, shelac wax, rice wax, jojoba wax, wax roses, jasmine wax or a combination thereof. 12. The lip composition according to claim 5, characterized in that it also comprises a colorant. The lip composition according to claim 5, characterized in that it further comprises an active ingredient that is selected from the group consisting of allantoin, aloe vera, elastin, collagen, vitamin E and derivatives thereof, vitamin A and derivatives of the same, liposomes, sodium hyaluronate, water, botanical extracts, phospholipids, silk powder, primrose oil, cholesteryl glutamate, hyaluronic acid, lauroyl acid, or a combination thereof. 14. The lip composition according to claim 5, characterized in that one hour after the application of the composition to the lips, there is an increase of about four times in the wetting of the lips. 15. The lip composition according to claim 5, characterized in that once applied to the lips, it provides a rapid exfoliation of the cracked cells in the lips. 16. A lip composition characterized in that it comprises from about 70 to about 90% by weight, with respect to the total weight of the lip composition, of a base including hydroxylated lanolin and polybutene, and from about 0.01 to about 15% by weight of protoplasm of yeast cells that is substantially free of yeast cell wall material. 17. The lip composition according to claim 16, characterized in that it further comprises from about 2.0 to about
9. 9.0% by weight of colorants. 18. The lip composition according to claim 16, characterized in that the base constitutes approximately 76% by weight. The lip composition according to claim 16, characterized in that the living yeast cell derivative constitutes approximately 0.5% by weight. 20. A method for treating the lips characterized in that it includes applying to the lips a composition comprising a derivative of living yeast cells and a suitable vehicle. 21. A lip composition characterized in that it comprises a derivative of living yeast cells and a suitable vehicle. 22. A lip composition characterized in that it comprises from about 70 to about 90% by weight, based on the total weight of the lip composition, of a base including hydroxylated lanolin and polybutene, and from about 0.01 to about 15% by weight of a derived from living yeast cells.