MXPA03009460A - Deteccion de bacterias formadoras de esporas. - Google Patents
Deteccion de bacterias formadoras de esporas.Info
- Publication number
- MXPA03009460A MXPA03009460A MXPA03009460A MXPA03009460A MXPA03009460A MX PA03009460 A MXPA03009460 A MX PA03009460A MX PA03009460 A MXPA03009460 A MX PA03009460A MX PA03009460 A MXPA03009460 A MX PA03009460A MX PA03009460 A MXPA03009460 A MX PA03009460A
- Authority
- MX
- Mexico
- Prior art keywords
- spore forming
- nucleic acid
- forming bacteria
- probe
- acid sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se divulgan metodos, sondas, cebadores y secuencias de acido nucleico para la identificacion sistematica de genes de esporulacion en bacterias formadas de esporas. Una sonda comprende una secuencia de acido nucleico capaz de formar un hibrido detectable con regiones altamente conservadas del gen spoOA de ciertas bacterias formadoras de esporas de especies de bacterias Bacillus y Clostridium, dicha secuencia de acido nucleico es incapaz de formar un hibrido detectable con el material genetico de bacterias no formadoras de esporas. La amplificacion de una porcion del gen spoOA a partir del ADN celular de las bacterias formadoras de esporas por una reaccion en cadena de polimerasa utilizando dicha sonda, cebador o secuencia de acido nucleico como miembro de un grupo de cebadores resulta en la generacion de un producto de ADN de 346-365 nucleotidos de largo detectable. Un metodo divulgado comprende a) la combinacion de una sonda marcada o etiquetada con una muestra, b) la hibridacion de la sonda con el gen spoOA de bacterias formadoras de esporas blanco; y c) la deteccion del producto hibridado.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US2001/015793 WO2002092853A1 (en) | 2001-05-15 | 2001-05-15 | Detection of spore forming bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXPA03009460A true MXPA03009460A (es) | 2004-04-02 |
Family
ID=21742576
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXPA03009460A MXPA03009460A (es) | 2001-05-15 | 2001-05-15 | Deteccion de bacterias formadoras de esporas. |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1390527A1 (es) |
| CN (1) | CN1507496A (es) |
| BR (1) | BR0117012A (es) |
| CA (1) | CA2443441A1 (es) |
| MX (1) | MXPA03009460A (es) |
| NO (1) | NO20035082D0 (es) |
| WO (1) | WO2002092853A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102304559B (zh) * | 2010-02-05 | 2013-03-20 | 山东出入境检验检疫局检验检疫技术中心 | 凝结芽孢杆菌的荧光定量pcr快速检测方法 |
| CN110869510A (zh) * | 2017-07-12 | 2020-03-06 | 埃科莱布美国股份有限公司 | 用于检测细菌孢子的快速方法 |
| CN109706238B (zh) * | 2017-10-26 | 2023-04-07 | 珠海岐微生物科技有限公司 | 一种老年性黄斑病变的检测与治疗方法 |
| CN110592243A (zh) * | 2019-09-24 | 2019-12-20 | 湖北广济药业股份有限公司 | 维生素B2中生产菌株残留活菌及孢子的qPCR检测方法及所用引物和探针 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3616395A (en) * | 1994-10-14 | 1996-05-06 | University College Of Wales Aberystwyth | Method of monitoring endospore-forming bacteria |
| US5928875A (en) * | 1998-05-27 | 1999-07-27 | Betzdearborn Inc. | Primers for the detection of spore forming bacteria |
| JP3121332B1 (ja) * | 1999-11-10 | 2000-12-25 | 日本甜菜製糖株式会社 | 胞子形成型クロストリジウム属細菌の検出法 |
-
2001
- 2001-05-15 BR BR0117012-0A patent/BR0117012A/pt not_active IP Right Cessation
- 2001-05-15 EP EP01939059A patent/EP1390527A1/en not_active Withdrawn
- 2001-05-15 CA CA002443441A patent/CA2443441A1/en not_active Abandoned
- 2001-05-15 CN CNA018232612A patent/CN1507496A/zh active Pending
- 2001-05-15 WO PCT/US2001/015793 patent/WO2002092853A1/en not_active Application Discontinuation
- 2001-05-15 MX MXPA03009460A patent/MXPA03009460A/es unknown
-
2003
- 2003-11-14 NO NO20035082A patent/NO20035082D0/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| NO20035082D0 (no) | 2003-11-14 |
| CA2443441A1 (en) | 2002-11-21 |
| EP1390527A1 (en) | 2004-02-25 |
| BR0117012A (pt) | 2004-08-03 |
| WO2002092853A1 (en) | 2002-11-21 |
| CN1507496A (zh) | 2004-06-23 |
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