MXPA02012686A - Control molecular de segregacion transgene y su escape mediante un sistema de bloqueo recuperable de funcion (rbf). - Google Patents

Control molecular de segregacion transgene y su escape mediante un sistema de bloqueo recuperable de funcion (rbf).

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Publication number
MXPA02012686A
MXPA02012686A MXPA02012686A MXPA02012686A MXPA02012686A MX PA02012686 A MXPA02012686 A MX PA02012686A MX PA02012686 A MXPA02012686 A MX PA02012686A MX PA02012686 A MXPA02012686 A MX PA02012686A MX PA02012686 A MXPA02012686 A MX PA02012686A
Authority
MX
Mexico
Prior art keywords
rbf
constructs
tgis
function
intron
Prior art date
Application number
MXPA02012686A
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English (en)
Inventor
Eija Pehu
Original Assignee
Unicrop Ltd
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Filing date
Publication date
Application filed by Unicrop Ltd filed Critical Unicrop Ltd
Publication of MXPA02012686A publication Critical patent/MXPA02012686A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8265—Transgene containment, e.g. gene dispersal
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Graft Or Block Polymers (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

La invencion esta relacionada con un metodo y un complejo de constructos de DNA para proporcionar un nivel incrementado de control de segregacion transgene en Organismos mulsticelulares que se reproducen sexualmente (SRMOs), los cuales son propensos a cruce-fuera con sus parientes tipo silvestre o cultivados. El metodo y constructos permiten al granjero reusar la cosecha transgenica sin riesgo. El sistema de RBF comprende ademas de uno o mas Transgenes de interes (TGls) que codifican productos de gene deseados, uno o mas Constructos bloqueadores (BC), y uno o mas medios controlados por el usuario para recuperar las funciones bloqueadas. El BC tiene la capacidad de bloquear al menos una funcion esencial para la supervivencia y/o reproduccion del SRMO. De preferencia, mas de un BC flanqueador los TGls son usados. El BC puede estar ubicado en estrecha proximidad al TGI, de preferencia en un intron o flanqueando los TGls. La funcion bloqueada es recuperable mediante intervenciones controladas por el usuario, de preferencia aplicables bajo condiciones confinadas. La intervencion es combinada con uno o mas Constructos recuperadores (RC). Se describen diferentes tipos de sistemas de RBF. Los sistemas de RBF doble y triple y de segregacion y BC insertado en intron, son las modalidades preferidas de la presente invencion. Las lnserciones transgenicas (TI) del sistema de RBF triple y sus interacciones se muestran en la Figura (10).
MXPA02012686A 2000-07-14 2001-07-16 Control molecular de segregacion transgene y su escape mediante un sistema de bloqueo recuperable de funcion (rbf). MXPA02012686A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/617,543 US6849776B1 (en) 2000-07-14 2000-07-14 Molecular control of transgene segregation and its escape by a recoverable block of function (RBF) system
PCT/FI2001/000670 WO2002006498A1 (en) 2000-07-14 2001-07-16 Molecular control of transgene segregation and its escape by a recoverable block of function (rbf) system

Publications (1)

Publication Number Publication Date
MXPA02012686A true MXPA02012686A (es) 2004-07-30

Family

ID=24474058

Family Applications (1)

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MXPA02012686A MXPA02012686A (es) 2000-07-14 2001-07-16 Control molecular de segregacion transgene y su escape mediante un sistema de bloqueo recuperable de funcion (rbf).

Country Status (17)

Country Link
US (2) US6849776B1 (es)
EP (1) EP1303628B1 (es)
CN (1) CN1446262A (es)
AT (1) ATE341635T1 (es)
AU (2) AU2001282177B2 (es)
BR (1) BR0112342A (es)
CA (1) CA2416063C (es)
CZ (1) CZ200364A3 (es)
DE (1) DE60123623D1 (es)
HU (1) HU225804B1 (es)
IL (1) IL153863A0 (es)
MX (1) MXPA02012686A (es)
NZ (1) NZ523630A (es)
PL (1) PL359717A1 (es)
RU (1) RU2270866C2 (es)
SK (1) SK452003A3 (es)
WO (1) WO2002006498A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7495148B2 (en) * 2000-07-14 2009-02-24 Unicrop Ltd Double recoverable block of function
ITMI20071291A1 (it) * 2007-06-28 2008-12-29 Univ Degli Studi Milano Sistema di contenimento transgenico attraverso l'inibizione recuperabile della germinazione in semi transgenici.
AU2009214710B2 (en) * 2008-02-15 2014-03-06 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event MON87769 and methods for detection thereof
US8367392B2 (en) * 2008-09-05 2013-02-05 Transalgae Ltd. Genetic transformation of algal and cyanobacteria cells by microporation
US20110081706A1 (en) * 2009-10-02 2011-04-07 TransAlgae Ltd Method and system for efficient harvesting of microalgae and cyanobacteria
WO2011090752A1 (en) * 2009-12-29 2011-07-28 The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations Male and female sterility lines used to make hybrids in genetically modified plants
WO2014128659A1 (en) * 2013-02-21 2014-08-28 Cellectis Method to counter-select cells or organisms by linking loci to nuclease components
CA3021316A1 (en) 2016-04-20 2017-10-26 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for nucleic acid expression and protein secretion in bacteroides
AU2017376780A1 (en) 2016-12-15 2019-07-04 Novome Biotechnologies, Inc. Compositions and methods for modulating growth of a genetically modified gut bacterial cell

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5689041A (en) * 1989-08-10 1997-11-18 Plant Gentic Systems N.V. Plants modified with barstar for fertility restoration
GB9216151D0 (en) 1992-07-29 1992-09-09 Ici Plc Containment of plant germplasm
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
JPH11500922A (ja) * 1995-03-03 1999-01-26 ノバルティス・アクチエンゲゼルシャフト 化学リガンドの存在下での受容体媒介トランス活性化による植物における遺伝子発現の制御
AUPN816196A0 (en) 1996-02-19 1996-03-14 Forbio Research Pty Ltd Regulation of eukaryotic gene expression
DK1141350T3 (da) 1998-12-22 2010-11-08 Dow Agrosciences Llc Fremgangsmåde og genetiske sammensætninger til begrænsning af udkrydsning og uønskede genstrømme i nytteplanter

Also Published As

Publication number Publication date
US6849776B1 (en) 2005-02-01
HUP0302995A3 (en) 2005-06-28
DE60123623D1 (de) 2006-11-16
CN1446262A (zh) 2003-10-01
AU8217701A (en) 2002-01-30
CZ200364A3 (cs) 2003-04-16
CA2416063A1 (en) 2002-01-24
ATE341635T1 (de) 2006-10-15
HUP0302995A2 (hu) 2004-05-28
IL153863A0 (en) 2003-07-31
PL359717A1 (en) 2004-09-06
BR0112342A (pt) 2003-09-02
NZ523630A (en) 2004-07-30
AU2001282177B2 (en) 2006-04-13
RU2270866C2 (ru) 2006-02-27
WO2002006498A1 (en) 2002-01-24
CA2416063C (en) 2012-07-10
HU225804B1 (en) 2007-09-28
EP1303628B1 (en) 2006-10-04
US20040025200A1 (en) 2004-02-05
EP1303628A1 (en) 2003-04-23
SK452003A3 (en) 2003-06-03

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