MX9701708A - Metodo de transfeccion con fosfato de calcio. - Google Patents

Metodo de transfeccion con fosfato de calcio.

Info

Publication number
MX9701708A
MX9701708A MX9701708A MX9701708A MX9701708A MX 9701708 A MX9701708 A MX 9701708A MX 9701708 A MX9701708 A MX 9701708A MX 9701708 A MX9701708 A MX 9701708A MX 9701708 A MX9701708 A MX 9701708A
Authority
MX
Mexico
Prior art keywords
calcium phosphate
phosphate transfection
methods
host cell
particles
Prior art date
Application number
MX9701708A
Other languages
English (en)
Inventor
Florian M Wurm
Martin Jordan
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/303,245 external-priority patent/US5484720A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX9701708A publication Critical patent/MX9701708A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

Se proporciona un método para la transfeccion con fosfato de calcio de una célula huésped eucariotica, en donde las partículas que comprenden fosfato de calcio y un ácido nucleico deseado se hacen crecer hasta una tamaño optimo y a continuacion se ponen en contacto con la célula huésped bajo condiciones que reducen sustancialmente la velocidad de crecimiento de la partícula y permiten que la célula huésped absorba las partículas para formar una célula transfectada.
MX9701708A 1994-09-08 1995-08-30 Metodo de transfeccion con fosfato de calcio. MX9701708A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/303,245 US5484720A (en) 1994-09-08 1994-09-08 Methods for calcium phosphate transfection
US08/430,533 US5593875A (en) 1994-09-08 1995-04-26 Methods for calcium phosphate transfection
PCT/US1995/011088 WO1996007750A1 (en) 1994-09-08 1995-08-30 Methods for calcium phosphate transfection

Publications (1)

Publication Number Publication Date
MX9701708A true MX9701708A (es) 1997-05-31

Family

ID=26973345

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9701708A MX9701708A (es) 1994-09-08 1995-08-30 Metodo de transfeccion con fosfato de calcio.

Country Status (12)

Country Link
EP (1) EP0779931B1 (es)
JP (1) JP3815794B2 (es)
AT (1) ATE228170T1 (es)
AU (1) AU694800B2 (es)
CA (1) CA2198589C (es)
DE (1) DE69528903T2 (es)
DK (1) DK0779931T3 (es)
ES (1) ES2187574T3 (es)
IL (1) IL115178A0 (es)
MX (1) MX9701708A (es)
PT (1) PT779931E (es)
WO (1) WO1996007750A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9515356D0 (en) 1995-07-26 1995-09-20 Medical Res Council Improvements in or relating to delivery of nucleic acid
DE69636755T2 (de) 1996-12-24 2007-10-11 Société des Produits Nestlé S.A. Immortalisierte menschliche Epithelzell-Linie
US6635457B1 (en) * 1999-07-30 2003-10-21 Stratagene Selection for more efficient transformation host cells
DE10024334B4 (de) * 2000-05-17 2006-06-01 Medigene Ag Verfahren zum Einbringen von Nukleinsäure in eine Zelle (Transfektion) mittels Calciumphosphat
WO2003000908A1 (en) * 2001-06-21 2003-01-03 Ecole Polytechnique Federale De Lausanne A method for introducing a nucleic acid into a eukaryotic cell
EP1270733A1 (en) * 2001-06-21 2003-01-02 Ecole Polytechnique Federale De Lausanne Method for introducing a nucleic acid into a eukaryotic cell
EP1487479B1 (en) 2001-10-22 2012-06-20 Merck Serono SA Mutant glycoproteins
US9493744B2 (en) * 2012-06-20 2016-11-15 Genentech, Inc. Methods for viral inactivation and other adventitious agents

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0430930B1 (en) * 1985-03-15 1993-09-01 Btg International Limited Factor ix protein

Also Published As

Publication number Publication date
CA2198589C (en) 2010-06-15
CA2198589A1 (en) 1996-03-14
EP0779931B1 (en) 2002-11-20
JP3815794B2 (ja) 2006-08-30
IL115178A0 (en) 1995-12-31
AU694800B2 (en) 1998-07-30
ES2187574T3 (es) 2003-06-16
EP0779931A1 (en) 1997-06-25
DE69528903D1 (de) 2003-01-02
AU3542995A (en) 1996-03-27
DK0779931T3 (da) 2003-03-03
ATE228170T1 (de) 2002-12-15
WO1996007750A1 (en) 1996-03-14
DE69528903T2 (de) 2003-07-24
JPH10508462A (ja) 1998-08-25
PT779931E (pt) 2003-04-30

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