MX9603866A - Process for treating eucaryotic cells. - Google Patents

Process for treating eucaryotic cells.

Info

Publication number
MX9603866A
MX9603866A MX9603866A MX9603866A MX9603866A MX 9603866 A MX9603866 A MX 9603866A MX 9603866 A MX9603866 A MX 9603866A MX 9603866 A MX9603866 A MX 9603866A MX 9603866 A MX9603866 A MX 9603866A
Authority
MX
Mexico
Prior art keywords
treating
eucaryotic cells
applications
lipopolysaccharides
toxicity
Prior art date
Application number
MX9603866A
Other languages
Spanish (es)
Inventor
Matthew Cotten
Adam Baker
Michael Buschle
Ernst Wagner
Tamas Schweighoffer
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Priority claimed from PCT/EP1994/000859 external-priority patent/WO1994021808A1/en
Publication of MX9603866A publication Critical patent/MX9603866A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

In a process for treating eucaryotic cells, in applications in which the toxicity of lipopolysaccharides has an adverse effect, substances are used during transfection or to clean the foreign material which bond lipopolysaccharides and neutralise their toxicity. The process is of particular advantage when foreign material is to be inserted in the cells, especially in gene transfer methods. Compositions containing a lipopolysaccharide-bonding substance are used in cell culture applications as a medium and in therapeutic applications as medicaments.
MX9603866A 1994-03-18 1994-03-18 Process for treating eucaryotic cells. MX9603866A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/EP1994/000859 WO1994021808A1 (en) 1993-03-19 1994-03-18 Process for preparing cancer vaccines
DE4415001A DE4415001A1 (en) 1994-04-29 1994-04-29 Treatment of eukaryotic cells with cpds. that bind lipo-polysaccharide
PCT/EP1995/000995 WO1995025808A1 (en) 1994-03-18 1995-03-17 Process for treating eucaryotic cells

Publications (1)

Publication Number Publication Date
MX9603866A true MX9603866A (en) 1997-03-29

Family

ID=6516773

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9603866A MX9603866A (en) 1994-03-18 1994-03-18 Process for treating eucaryotic cells.

Country Status (6)

Country Link
EP (1) EP0750677A1 (en)
JP (1) JPH09510354A (en)
CA (1) CA2183647A1 (en)
DE (1) DE4415001A1 (en)
MX (1) MX9603866A (en)
WO (1) WO1995025808A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110020924A1 (en) * 2008-04-30 2011-01-27 Gradalis, Inc. Highly pure plasmid dna preparations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2037727A1 (en) * 1991-03-07 1992-09-08 Burton W. Blais Removing lps pyrogen from aqueous solution
CA2037726A1 (en) * 1991-03-07 1992-09-08 Burton W. Blais Wound dressing of hydrophobic macroporous cloth and oligopeptides
NZ244306A (en) * 1991-09-30 1995-07-26 Boehringer Ingelheim Int Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation

Also Published As

Publication number Publication date
CA2183647A1 (en) 1995-09-28
DE4415001A1 (en) 1995-11-02
JPH09510354A (en) 1997-10-21
WO1995025808A1 (en) 1995-09-28
EP0750677A1 (en) 1997-01-02

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