MX9603864A - Uso de un inhibidor recombinante de erythrina caffra para la purificacion de serina proteasas. - Google Patents

Uso de un inhibidor recombinante de erythrina caffra para la purificacion de serina proteasas.

Info

Publication number
MX9603864A
MX9603864A MX9603864A MX9603864A MX9603864A MX 9603864 A MX9603864 A MX 9603864A MX 9603864 A MX9603864 A MX 9603864A MX 9603864 A MX9603864 A MX 9603864A MX 9603864 A MX9603864 A MX 9603864A
Authority
MX
Mexico
Prior art keywords
inhibitor
serine protease
purifying
serine proteases
polypeptide
Prior art date
Application number
MX9603864A
Other languages
English (en)
Inventor
Ulrich Kohnert
Anne Stern
Stephan Fischer
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4424171A external-priority patent/DE4424171A1/de
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of MX9603864A publication Critical patent/MX9603864A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Un proceso para la purificacion de las serina proteasas de una mezcla de proteína, uniendo la serina proteasa a un polipéptido inmovilizado con la actividad de un inhibidor DE-3 de Erythrina caffra, eliminando los componentes sin unir de la mezcla de proteína, separando la serina proteasa del inhibidor y separa el inhibidor inmovilizado de la serina proteasa soluble y aislar la serina proteasa, el cual está caracterizada porque se utiliza un polipéptido como el polipéptido el cual es el producto de una expresion procariotica o eucariotica de un ácido nucleico exogeno. Este inhibidor se distingue por una actividad específica mejorada y es particularmente adecuado para la purificacion de activadores del plasminogeno, tales como los activadores de plasminogeno de tejido (t-PA y derivados).
MX9603864A 1994-03-16 1995-03-13 Uso de un inhibidor recombinante de erythrina caffra para la purificacion de serina proteasas. MX9603864A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4408939 1994-03-16
DE4424171A DE4424171A1 (de) 1994-03-16 1994-07-08 Verwendung von rekombinantem Inhibitor aus Erythrina caffra zur Reinigung von Serinproteasen
PCT/EP1995/000926 WO1995025168A1 (de) 1994-03-16 1995-03-13 Verwendung von rekombinantem inhibitor aus erythrina caffra zur reinigung von serinproteasen

Publications (1)

Publication Number Publication Date
MX9603864A true MX9603864A (es) 1997-03-29

Family

ID=25934769

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9603864A MX9603864A (es) 1994-03-16 1995-03-13 Uso de un inhibidor recombinante de erythrina caffra para la purificacion de serina proteasas.

Country Status (14)

Country Link
US (1) US5958722A (es)
EP (1) EP0750667B1 (es)
JP (1) JP2846961B2 (es)
AT (1) ATE174962T1 (es)
AU (1) AU691904B2 (es)
CA (1) CA2185436C (es)
CZ (1) CZ285502B6 (es)
ES (1) ES2128721T3 (es)
IL (1) IL112995A (es)
MX (1) MX9603864A (es)
NO (1) NO316704B1 (es)
NZ (1) NZ282790A (es)
SK (1) SK282430B6 (es)
WO (1) WO1995025168A1 (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0112122B1 (en) * 1982-12-14 1991-08-28 South African Inventions Development Corporation Plasminogen activator
AU597908B2 (en) * 1985-10-04 1990-06-14 Implico B.V. A protein reagent having the ability to bind tissue plasminogen activator in its two chain form and/or its single chain form

Also Published As

Publication number Publication date
CA2185436A1 (en) 1995-09-21
CA2185436C (en) 2000-08-01
AU2069395A (en) 1995-10-03
IL112995A (en) 2004-07-25
US5958722A (en) 1999-09-28
EP0750667B1 (de) 1998-12-23
NO316704B1 (no) 2004-04-13
WO1995025168A1 (de) 1995-09-21
IL112995A0 (en) 1995-06-29
JP2846961B2 (ja) 1999-01-13
CZ252996A3 (en) 1996-11-13
SK114296A3 (en) 1997-05-07
JPH09503395A (ja) 1997-04-08
ES2128721T3 (es) 1999-05-16
NO963849D0 (no) 1996-09-13
CZ285502B6 (cs) 1999-08-11
ATE174962T1 (de) 1999-01-15
EP0750667A1 (de) 1997-01-02
SK282430B6 (sk) 2002-02-05
AU691904B2 (en) 1998-05-28
NZ282790A (en) 1997-08-22
NO963849L (no) 1996-11-15

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