MX9304354A - NUCLEIC ACID DETECTION. - Google Patents

NUCLEIC ACID DETECTION.

Info

Publication number
MX9304354A
MX9304354A MX9304354A MX9304354A MX9304354A MX 9304354 A MX9304354 A MX 9304354A MX 9304354 A MX9304354 A MX 9304354A MX 9304354 A MX9304354 A MX 9304354A MX 9304354 A MX9304354 A MX 9304354A
Authority
MX
Mexico
Prior art keywords
nucleic acid
acid detection
signal
enhancers
probe
Prior art date
Application number
MX9304354A
Other languages
Spanish (es)
Inventor
Michael Lee Cubbage
Joel Bresser
Negindra Prashad
Mark Blick
William Dugald Weber
Shyh Chen Ju
Morteza Asgari
David Colvin
Rebecca Jurtshuk
Original Assignee
Aprogenex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/916,068 external-priority patent/US5521061A/en
Application filed by Aprogenex Inc filed Critical Aprogenex Inc
Publication of MX9304354A publication Critical patent/MX9304354A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se describen procesos y composiciones que mejoran la velocidad y/osensibilidad de la hibridación de ácido nucléico, especialmente in situ:mejoradores de permeación y resaltadores de señal para resaltar la señalde una sonda fluorecente, reductores de fondo, fondas como grupossoportadores múltiples y poblaciones de sondas dirigidas a la dos hebrasde un objetivo.Processes and compositions are described that improve the speed and / or osensitivity of nucleic acid hybridization, especially in situ: permeation enhancers and signal enhancers to highlight signal from a fluorescent probe, background reducers, pools such as multiple carrier groups, and probe populations. directed at the two strands of a target.

MX9304354A 1992-07-17 1993-07-19 NUCLEIC ACID DETECTION. MX9304354A (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US91618392A 1992-07-17 1992-07-17
US91589492A 1992-07-17 1992-07-17
US91592792A 1992-07-17 1992-07-17
US91589392A 1992-07-17 1992-07-17
US91590092A 1992-07-17 1992-07-17
US07/916,068 US5521061A (en) 1992-07-17 1992-07-17 Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies

Publications (1)

Publication Number Publication Date
MX9304354A true MX9304354A (en) 1995-01-31

Family

ID=27560342

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9304354A MX9304354A (en) 1992-07-17 1993-07-19 NUCLEIC ACID DETECTION.

Country Status (4)

Country Link
AU (1) AU4775193A (en)
IL (2) IL106380A0 (en)
MX (1) MX9304354A (en)
WO (1) WO1994002644A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2792332B1 (en) * 1999-04-14 2001-07-27 Labonord PROCESS FOR THE PREPARATION OF A CYTOLOGICAL SUSPENSION
DE10006662A1 (en) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Sample vessel for stabilizing and isolating nucleic acid, contains a lytic solution that stabilizes nucleic acid and a solid phase that binds it, especially for sampling whole blood
DK3247710T3 (en) 2015-01-20 2022-11-14 Biosearch Tech Inc Coumarin-based compounds and related methods
EP3289098B1 (en) * 2015-04-30 2024-03-20 Sakura Finetek U.S.A., Inc. Methods to reduce evaporation during elevated temperature
JP6550471B2 (en) * 2016-04-15 2019-07-24 サクラ ファインテック ユー．エス．エー．， インコーポレイテッド Evaporation reduction method and reagent used therefor

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5166195A (en) * 1990-05-11 1992-11-24 Isis Pharmaceuticals, Inc. Antisense inhibitors of the human immunodeficiency virus phosphorothioate oligonucleotides

Also Published As

Publication number Publication date
IL106381A0 (en) 1993-11-15
AU4775193A (en) 1994-02-14
WO1994002644A1 (en) 1994-02-03
IL106380A0 (en) 1993-11-15

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