MX374620B - Método para selección de célula productora a largo plazo usando acilación de histona como marcadores. - Google Patents

Método para selección de célula productora a largo plazo usando acilación de histona como marcadores.

Info

Publication number
MX374620B
MX374620B MX2016014542A MX2016014542A MX374620B MX 374620 B MX374620 B MX 374620B MX 2016014542 A MX2016014542 A MX 2016014542A MX 2016014542 A MX2016014542 A MX 2016014542A MX 374620 B MX374620 B MX 374620B
Authority
MX
Mexico
Prior art keywords
long
markers
cell selection
producer cell
term producer
Prior art date
Application number
MX2016014542A
Other languages
English (en)
Other versions
MX2016014542A (es
Inventor
Benjamin Moritz
Peter Becker
Ulrich Goepfert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2016014542A publication Critical patent/MX2016014542A/es
Publication of MX374620B publication Critical patent/MX374620B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

En la presente se da a conocer un método para determinar la metilación de un ácido nucleico promotor operablemente enlazado a un ácido nucleico que codifica para un polipéptido y determinar de este modo la productividad a largo plazo de una célula. También un aspecto es método para seleccionar una célula para producir un polipéptido al determinar la metilación del ácido nucleico promotor operablemente enlazado al gen estructural que codifica para el polipéptido.
MX2016014542A 2014-05-19 2015-05-13 Método para selección de célula productora a largo plazo usando acilación de histona como marcadores. MX374620B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14168896 2014-05-19
PCT/EP2015/060593 WO2015177022A1 (en) 2014-05-19 2015-05-13 Method for the selection of a long-term producing cell using histone acylation as markers

Publications (2)

Publication Number Publication Date
MX2016014542A MX2016014542A (es) 2017-02-20
MX374620B true MX374620B (es) 2025-03-06

Family

ID=50735934

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016014542A MX374620B (es) 2014-05-19 2015-05-13 Método para selección de célula productora a largo plazo usando acilación de histona como marcadores.

Country Status (13)

Country Link
US (2) US11332797B2 (es)
EP (1) EP3146052B1 (es)
JP (1) JP6649276B2 (es)
KR (1) KR102320614B1 (es)
CN (1) CN106414745B (es)
BR (1) BR112016025972B1 (es)
CA (1) CA2944813A1 (es)
ES (1) ES2708830T3 (es)
MX (1) MX374620B (es)
PL (1) PL3146052T3 (es)
RU (1) RU2707543C2 (es)
TR (1) TR201819532T4 (es)
WO (1) WO2015177022A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106414745B (zh) * 2014-05-19 2019-08-16 豪夫迈·罗氏有限公司 使用组蛋白酰化作为标记来选择长期生产性细胞的方法
WO2025108794A1 (en) 2023-11-24 2025-05-30 Evonik Operations Gmbh Predicting the fitness of a cho cell
WO2025108795A1 (en) 2023-11-24 2025-05-30 Evonik Operations Gmbh A method of classifying cho cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004016018A (ja) 2002-06-12 2004-01-22 Japan Science & Technology Corp 染色体末端構造を制御するヒストンアセチル化酵素とヒストン脱アセチル化酵素
AU2003290453A1 (en) * 2002-12-20 2004-07-14 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
JP5283104B2 (ja) 2007-01-10 2013-09-04 独立行政法人理化学研究所 アセチル化及び脱アセチル化の蛍光可視化検出方法
RU2425882C1 (ru) 2009-11-10 2011-08-10 Учреждение Российской Академии Наук Институт Биологии Гена Ран Способ создания трансгенных линий клеток млекопитающего со стабильным и высоким уровнем экспрессии трансгенного белка
WO2011128377A1 (en) * 2010-04-14 2011-10-20 F. Hoffmann-La Roche Ag Method for the selection of a long-term producing cell
CN106414745B (zh) * 2014-05-19 2019-08-16 豪夫迈·罗氏有限公司 使用组蛋白酰化作为标记来选择长期生产性细胞的方法

Also Published As

Publication number Publication date
JP6649276B2 (ja) 2020-02-19
US20170081732A1 (en) 2017-03-23
MX2016014542A (es) 2017-02-20
KR102320614B1 (ko) 2021-11-02
WO2015177022A9 (en) 2016-11-17
CN106414745B (zh) 2019-08-16
RU2016149090A3 (es) 2018-12-24
CA2944813A1 (en) 2015-11-26
WO2015177022A1 (en) 2015-11-26
CN106414745A (zh) 2017-02-15
RU2707543C2 (ru) 2019-11-27
BR112016025972B1 (pt) 2022-04-19
KR20170003696A (ko) 2017-01-09
US11332797B2 (en) 2022-05-17
PL3146052T3 (pl) 2019-04-30
TR201819532T4 (tr) 2019-01-21
US20230016623A1 (en) 2023-01-19
RU2016149090A (ru) 2018-06-20
EP3146052B1 (en) 2018-11-28
EP3146052A1 (en) 2017-03-29
JP2017515489A (ja) 2017-06-15
BR112016025972A2 (pt) 2018-02-20
ES2708830T3 (es) 2019-04-11

Similar Documents

Publication Publication Date Title
AR122864A2 (es) Variantes de lipasa y polinucleótidos que las codifican
PL3926051T3 (pl) Sposoby zbierania hodowli komórek ssaczych
CL2013002068A1 (es) Linea celular de ratón con genes inactivados nav1.7 global viable; metodo para generar ratón con dichos genes inactivados; hibridoma.
MX2020009401A (es) Ligadores de nucleótidos modificados.
EP3194578A4 (en) Immune-compatible cells created by nuclease-mediated editing of genes encoding hla
BR112016018105A2 (pt) célula eletroquímica, método de produção de uma célula eletroquímica
HUE051069T2 (hu) Eljárás grafén elõállítására, grafit elektródák elektrokémiai eróziójával
PT3165609T (pt) Cartucho de expressão para a transformação de uma célula eucariótica, método para transformar uma célula eucariótica, organismo geneticamente modificado e processo para a produção de biocombustíveis e/ou bioquímicos e biocombustíveis assim produzidos
BR112015023472A2 (pt) método de produção de um produto químico, método de bioprodução de um produto químico, organismo geneticamente modificado e produto
MX2017013136A (es) Transaminasas mutantes asi como metodos y usos relacionados con ellas.
EA201791275A1 (ru) Способы получения ctp-модифицированных полипептидов длительного действия
MX2020003508A (es) Un factor de secrecion de proteinas con alta eficacia secretora y un vector de expresion que lo comprende.
IN2014MU02667A (es)
PT3170896T (pt) Método de produção de células estaminais pluripotentes possuindo um gene do recetor das células t específico para um antigénio
IL253071A0 (en) Methods for growing mammalian cells
MY183321A (en) Microorganism producing o-acetyl-homoserine and method for producing o-acetylhomoserine using the same
JO3255B1 (ar) طريقة لإنتاج أحماض l- أميني
HUE063273T2 (hu) Heteromultimer fehérjék elõállítása emlõssejtek felhasználásával
PE20170329A1 (es) Polipeptidos que tienen actividad xilanasa con una tasa de conversion alta de polisacaridos que contienen xilosa
EP3133148A4 (en) Method for mass culturing photosynthetic microalgae by additionally supplying environmental water
TR201904928T4 (tr) Tereftalik asit diesterlerin resirküle alkol dehidratasyonu ile üretimi için yöntem.
MX374620B (es) Método para selección de célula productora a largo plazo usando acilación de histona como marcadores.
BR112019000209A2 (pt) métodos de produção de um produto de fermentação em trichoderma
EP3064578A4 (en) Method of producing cell population with high target cell purity
BR112017012389A2 (pt) supressão de rnai parental de gene kruppel para controlar pragas de coleóptero

Legal Events

Date Code Title Description
FG Grant or registration