MX354561B - Proceso enzimatico para la hidrolisis de grasa y aceite. - Google Patents

Proceso enzimatico para la hidrolisis de grasa y aceite.

Info

Publication number
MX354561B
MX354561B MX2014009145A MX2014009145A MX354561B MX 354561 B MX354561 B MX 354561B MX 2014009145 A MX2014009145 A MX 2014009145A MX 2014009145 A MX2014009145 A MX 2014009145A MX 354561 B MX354561 B MX 354561B
Authority
MX
Mexico
Prior art keywords
regio
fats
fat
enzymatic process
oil hydrolysis
Prior art date
Application number
MX2014009145A
Other languages
English (en)
Other versions
MX2014009145A (es
Inventor
Anil Odaneth Annamma
Natwarial Vadgama Rajesh
Devdas Bhat Anuradha
Pande Amit
Anil Warke Mrunal
Original Assignee
Arvind Mallinath Lali
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arvind Mallinath Lali filed Critical Arvind Mallinath Lali
Publication of MX2014009145A publication Critical patent/MX2014009145A/es
Publication of MX354561B publication Critical patent/MX354561B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

La presente invención se refiere a un proceso para la producción de ácidos grasos, monoacilglicerol (MAG) sn-regio, diacilgliceroles (DAG) sn-regio y glicerol, que comprende: preparar una mezcla homogénea de grasa, disolvente polar orgánico y agua, en donde la mezcla homogénea está preparada mediante la mezcla de grasa, un solvente orgánico polar y agua en una proporción de1:4: 0.15 1:7:0.5, bajo condiciones para formar un sistema de fase simple; someter la mezcla homogénea a una primera hidrólisis enzimática con una lipasa inmovilizada para obtener un hidrolizado parcial que comprende ácidos grasos, MAG, DAG y glicerol; someter el hidrolizado parcial a una resina de intercambio iónico para obtener un primer producto que comprende MAG y DAG; y someter el primero producto a una segunda hidrólisis enzimática con la lipasa inmovilizada para obtener un segundo producto que comprende ácidos grasos, glicerol, y menos 5% de MAG; en donde el solvente orgánico polar se selecciona del grupo que consiste de t-butanol, isoamil alcohol, diacetona alcohol, etanol, propanol y t-pentanol.
MX2014009145A 2012-01-30 2013-01-30 Proceso enzimatico para la hidrolisis de grasa y aceite. MX354561B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN278MU2012 2012-01-30
PCT/IB2013/000110 WO2013114178A1 (en) 2012-01-30 2013-01-30 Enzymatic process for fat and oil hydrolysis

Publications (2)

Publication Number Publication Date
MX2014009145A MX2014009145A (es) 2015-08-20
MX354561B true MX354561B (es) 2018-03-09

Family

ID=47884416

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014009145A MX354561B (es) 2012-01-30 2013-01-30 Proceso enzimatico para la hidrolisis de grasa y aceite.

Country Status (13)

Country Link
US (1) US9512451B2 (es)
EP (1) EP2809789B1 (es)
KR (1) KR20140145578A (es)
CN (1) CN104245949B (es)
AU (1) AU2013213921B2 (es)
BR (1) BR112014018682A8 (es)
CA (1) CA2863162A1 (es)
CL (1) CL2014002010A1 (es)
DK (1) DK2809789T3 (es)
MX (1) MX354561B (es)
PH (1) PH12014501720A1 (es)
SG (1) SG11201404463PA (es)
WO (1) WO2013114178A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2527479B (en) 2013-04-26 2020-10-14 Desbarats Andrew A proppant immobilized enzyme and a viscofied fracture fluid
GB2528111A (en) * 2014-07-10 2016-01-13 Breathe Ad Ltd Treatment method for fats, oils and/or greases
US9688943B2 (en) 2015-05-29 2017-06-27 beauty Avenues LLC Candle containing non-ionic emulsifer
EP3461901A1 (en) 2017-09-29 2019-04-03 Technische Universität München A process for the cell-free enzymatic production of 10-hydroxystearic acid (10-hsa) from bio-based oils for lubricant formulation
ES2968424T3 (es) * 2017-10-13 2024-05-09 Glycosbio Inc Aceite procesado que comprende monoacilglicéridos
SG10201900696YA (en) * 2019-01-25 2020-08-28 Wilmar International Ltd A process for hydrolyzing oil with high melting point by lipase
CN111004821A (zh) * 2019-12-31 2020-04-14 安徽省瑞芬得油脂深加工有限公司 一种环境友好的脂肪酸制备方法
CN112852620B (zh) * 2020-12-30 2023-11-21 苏州汇通色谱分离纯化有限公司 一种通用型准固定化酶反应器
CN116042736A (zh) * 2023-02-24 2023-05-02 江南大学 一种甘油二酯的酶法生产工艺
CN116445558A (zh) * 2023-02-24 2023-07-18 江南大学 一种甘油二酯油的制备方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629742A (en) * 1986-01-27 1986-12-16 Akzo America Inc. Hydrolysis of fats
WO1990004033A1 (en) 1988-10-04 1990-04-19 Enzytech, Inc. Production of monoglycerides by enzymatic transesterification
US5935828A (en) 1989-05-01 1999-08-10 Opta Food Ingredients, Inc. Enzymatic production of monoglycerides containing omega-3 unsaturated fatty acids
US5116745A (en) 1990-04-19 1992-05-26 The Procter & Gamble Company Process for preparing 2-acylglycerides or 1,2-diacyl diglycerides or 2,3-diacyl diglycerides
US5932458A (en) 1992-03-23 1999-08-03 The United States Of America As Represented By The Secretary Of Agriculture Method of rapid fat and oil splitting using a lipase catalyst found in seeds
US6500974B2 (en) 2001-02-28 2002-12-31 Council Of Scientific And Industrial Research Process for the preparation of a monoglyceride
DE50113375D1 (de) * 2001-06-21 2008-01-24 T & T Oleochemie Gmbh Verfahren zur enzymatischen Spaltung von Ölen und Fetten
US7709667B2 (en) * 2005-04-28 2010-05-04 Kao Corporation Process for producing fat or oil
JP4915732B2 (ja) * 2006-12-15 2012-04-11 花王株式会社 固定化酵素を用いた有用物質の製造方法
CN101270375B (zh) * 2008-05-22 2011-11-09 清华大学 叔丁醇体系中酶法制备1，3-甘油二酯的工艺
CN102676304B (zh) 2011-03-15 2013-06-19 清华大学 一种生物柴油的制备方法

Also Published As

Publication number Publication date
CL2014002010A1 (es) 2015-04-24
DK2809789T3 (da) 2019-01-02
EP2809789B1 (en) 2018-09-12
US20150010966A1 (en) 2015-01-08
MX2014009145A (es) 2015-08-20
WO2013114178A1 (en) 2013-08-08
CN104245949B (zh) 2017-10-13
EP2809789A1 (en) 2014-12-10
CN104245949A (zh) 2014-12-24
KR20140145578A (ko) 2014-12-23
BR112014018682A2 (es) 2017-06-20
SG11201404463PA (en) 2014-10-30
BR112014018682A8 (pt) 2017-07-11
PH12014501720B1 (en) 2014-11-10
AU2013213921B2 (en) 2017-05-18
US9512451B2 (en) 2016-12-06
CA2863162A1 (en) 2013-08-08
PH12014501720A1 (en) 2014-11-10
AU2013213921A1 (en) 2014-08-28

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