MX2023001944A - Producción microbiana de cannabinoides. - Google Patents

Producción microbiana de cannabinoides.

Info

Publication number
MX2023001944A
MX2023001944A MX2023001944A MX2023001944A MX2023001944A MX 2023001944 A MX2023001944 A MX 2023001944A MX 2023001944 A MX2023001944 A MX 2023001944A MX 2023001944 A MX2023001944 A MX 2023001944A MX 2023001944 A MX2023001944 A MX 2023001944A
Authority
MX
Mexico
Prior art keywords
cannabinoids
cannabinoid
microbial production
synthase
disclosure
Prior art date
Application number
MX2023001944A
Other languages
English (en)
Inventor
Yi Xiong
Andrew P Klein
Victor F Holmes
John E Hung
Irina A Koryakina
William E Draper
Hailley S Warbington
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of MX2023001944A publication Critical patent/MX2023001944A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
    • C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
    • C12Y121/03008—Cannabidiolic acid synthase (1.21.3.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01206—3,5,7-Trioxododecanoyl-CoA synthase (2.3.1.206)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y404/00—Carbon-sulfur lyases (4.4)
    • C12Y404/01—Carbon-sulfur lyases (4.4.1)
    • C12Y404/01026—Olivetolic acid cyclase (4.4.1.26)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Las composiciones y métodos de la descripción pueden usarse para producir un cannabinoide en una célula hospedadora, tal como una célula de levadura. Por ejemplo, la descripción presenta células hospedadoras (por ejemplo, células de levadura) modificadas para expresar una o más enzimas de una vía biosintética cannabinoide, tal como una enzima activadora de acilos (AAE), un tetracétido sintasa (TKS), una sintasa de ácido cannabigerólico (CB-GaS) y/o una ciclasa de ácido olivetólico (OAC).
MX2023001944A 2020-08-19 2021-08-19 Producción microbiana de cannabinoides. MX2023001944A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063067633P 2020-08-19 2020-08-19
PCT/US2021/046780 WO2022040475A1 (en) 2020-08-19 2021-08-19 Microbial production of cannabinoids

Publications (1)

Publication Number Publication Date
MX2023001944A true MX2023001944A (es) 2023-04-05

Family

ID=80323300

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023001944A MX2023001944A (es) 2020-08-19 2021-08-19 Producción microbiana de cannabinoides.

Country Status (8)

Country Link
US (1) US20230304049A1 (es)
EP (1) EP4200426A4 (es)
CN (1) CN116391033A (es)
BR (1) BR112023002818A2 (es)
CA (1) CA3188362A1 (es)
IL (1) IL300490A (es)
MX (1) MX2023001944A (es)
WO (1) WO2022040475A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022256697A1 (en) * 2021-06-04 2022-12-08 Amyris, Inc. Methods of purifying cannabinoids
WO2023183857A1 (en) * 2022-03-23 2023-09-28 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
CN115644057A (zh) * 2022-09-22 2023-01-31 广东省农业科学院环境园艺研究所 快速扩繁剑叶文心兰的培养基组合及方法
WO2024112694A1 (en) * 2022-11-21 2024-05-30 Willow Biosciences, Inc. Recombinant polypeptides with prenyltransferase activity for biosynthesis of cannabinoids and hop compounds

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013006953A1 (en) * 2011-07-13 2013-01-17 National Research Council Of Canada Genes and proteins for alkanoyl-coa synthesis
US10954534B2 (en) * 2014-07-14 2021-03-23 Librede Inc. Production of cannabigerolic acid in yeast
JP7198555B2 (ja) * 2017-04-27 2023-01-04 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア カンナビノイドおよびカンナビノイド誘導体を産生するための微生物および方法
CA3062645A1 (en) * 2017-05-10 2018-11-15 Baymedica, Inc. Recombinant production systems for prenylated polyketides of the cannabinoid family
WO2020102541A1 (en) * 2018-11-14 2020-05-22 Manus Bio, Inc. Microbial cells and methods for producing cannabinoids
WO2020161682A1 (en) * 2019-02-10 2020-08-13 Dyadic International (Usa), Inc. Production of cannabinoids in filamentous fungi
MX2021014054A (es) * 2019-05-22 2021-12-10 Hyasynth Biologicals Inc Metodos y celulas para la produccion microbiana de fitocannabinoides y precursores de fitocannabinoides.

Also Published As

Publication number Publication date
US20230304049A1 (en) 2023-09-28
WO2022040475A1 (en) 2022-02-24
EP4200426A4 (en) 2024-10-16
CA3188362A1 (en) 2022-02-24
WO2022040475A8 (en) 2022-12-22
IL300490A (en) 2023-04-01
BR112023002818A2 (pt) 2023-04-25
EP4200426A1 (en) 2023-06-28
CN116391033A (zh) 2023-07-04

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