MX2022013879A - Selección por inserción génica en genes esenciales. - Google Patents
Selección por inserción génica en genes esenciales.Info
- Publication number
- MX2022013879A MX2022013879A MX2022013879A MX2022013879A MX2022013879A MX 2022013879 A MX2022013879 A MX 2022013879A MX 2022013879 A MX2022013879 A MX 2022013879A MX 2022013879 A MX2022013879 A MX 2022013879A MX 2022013879 A MX2022013879 A MX 2022013879A
- Authority
- MX
- Mexico
- Prior art keywords
- knock
- essential gene
- coding sequence
- essential
- sequence
- Prior art date
Links
- 108700039887 Essential Genes Proteins 0.000 title abstract 6
- 238000003198 gene knock in Methods 0.000 title 1
- 108091026890 Coding region Proteins 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0636—T lymphocytes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0646—Natural killers cells [NK], NKT cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Bioinformatics & Computational Biology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Estrategias, sistemas, composiciones y métodos para la producción eficaz de clones celulares con inserción génica sin genes indicadores. Un gen esencial se establece como diana usando un casete de inserción génica que comprende una secuencia de codificación exógena de un producto génico de interés (o "secuencia de carga") en marco con y cadena abajo (3') de una secuencia de codificación exógena o una secuencia de codificación parcial del gen esencial. Los eventos de direccionamiento no deseados crean una versión no funcional del gen esencial, en esencia una inactivación (¿knock out¿), que se "rescata" mediante la integración correcta del casete de inserción génica, que restaura la región de codificación del gen esencial para que se produzca un producto génico funcional y coloca la secuencia de carga en marco con y cadena abajo de la secuencia de codificación del gen esencial.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202063019950P | 2020-05-04 | 2020-05-04 | |
PCT/US2021/030744 WO2021226151A2 (en) | 2020-05-04 | 2021-05-04 | Selection by essential-gene knock-in |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2022013879A true MX2022013879A (es) | 2023-02-01 |
Family
ID=78468371
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2022013879A MX2022013879A (es) | 2020-05-04 | 2021-05-04 | Selección por inserción génica en genes esenciales. |
Country Status (11)
Country | Link |
---|---|
US (2) | US20230227856A1 (es) |
EP (1) | EP4146813A2 (es) |
JP (1) | JP2023524976A (es) |
KR (1) | KR20230029603A (es) |
CN (1) | CN115916968A (es) |
AU (1) | AU2021267334A1 (es) |
BR (1) | BR112022022384A2 (es) |
CA (1) | CA3182286A1 (es) |
IL (1) | IL297881A (es) |
MX (1) | MX2022013879A (es) |
WO (1) | WO2021226151A2 (es) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11661459B2 (en) | 2020-12-03 | 2023-05-30 | Century Therapeutics, Inc. | Artificial cell death polypeptide for chimeric antigen receptor and uses thereof |
EP4263600A1 (en) | 2020-12-18 | 2023-10-25 | Century Therapeutics, Inc. | Chimeric antigen receptor systems with adaptable receptor specificity |
TW202400252A (zh) | 2022-04-28 | 2024-01-01 | 美商藍岩醫療公司 | 用於安全基因組整合之新位點及其使用方法 |
WO2023220206A2 (en) * | 2022-05-10 | 2023-11-16 | Editas Medicine, Inc. | Genome editing of b cells |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2015345979A1 (en) * | 2014-11-10 | 2017-06-01 | Murdoch Childrens Research Institute | Vectors and methods for targeted integration in loci comprising constitutively expressed genes |
CN109415687A (zh) * | 2016-04-07 | 2019-03-01 | 蓝鸟生物公司 | 嵌合抗原受体t细胞组合物 |
EP3652312A1 (en) * | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
-
2021
- 2021-05-04 WO PCT/US2021/030744 patent/WO2021226151A2/en active Search and Examination
- 2021-05-04 BR BR112022022384A patent/BR112022022384A2/pt unknown
- 2021-05-04 KR KR1020227040920A patent/KR20230029603A/ko active Search and Examination
- 2021-05-04 CN CN202180046858.XA patent/CN115916968A/zh active Pending
- 2021-05-04 AU AU2021267334A patent/AU2021267334A1/en active Pending
- 2021-05-04 IL IL297881A patent/IL297881A/en unknown
- 2021-05-04 JP JP2022567268A patent/JP2023524976A/ja active Pending
- 2021-05-04 EP EP21800128.7A patent/EP4146813A2/en active Pending
- 2021-05-04 CA CA3182286A patent/CA3182286A1/en active Pending
- 2021-05-04 MX MX2022013879A patent/MX2022013879A/es unknown
- 2021-05-04 US US17/923,358 patent/US20230227856A1/en active Pending
-
2023
- 2023-12-12 US US18/537,754 patent/US20240117383A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20240117383A1 (en) | 2024-04-11 |
US20230227856A1 (en) | 2023-07-20 |
EP4146813A2 (en) | 2023-03-15 |
CN115916968A (zh) | 2023-04-04 |
AU2021267334A1 (en) | 2022-12-22 |
KR20230029603A (ko) | 2023-03-03 |
IL297881A (en) | 2023-01-01 |
JP2023524976A (ja) | 2023-06-14 |
WO2021226151A3 (en) | 2021-12-02 |
WO2021226151A2 (en) | 2021-11-11 |
CA3182286A1 (en) | 2021-11-11 |
BR112022022384A2 (pt) | 2022-12-13 |
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