MX2022005573A - Molecula de acido nucleico del evento transgenico de maiz me240913 que expresa la proteina cry1da, celula, planta y semilla transgenica, usos de las mismas, producto de planta, metodo, kit y amplicon para la deteccion del evento, y metodos para producir una planta transgenica y de control de insectos plagas lepidopteros. - Google Patents

Molecula de acido nucleico del evento transgenico de maiz me240913 que expresa la proteina cry1da, celula, planta y semilla transgenica, usos de las mismas, producto de planta, metodo, kit y amplicon para la deteccion del evento, y metodos para producir una planta transgenica y de control de insectos plagas lepidopteros.

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Publication number
MX2022005573A
MX2022005573A MX2022005573A MX2022005573A MX2022005573A MX 2022005573 A MX2022005573 A MX 2022005573A MX 2022005573 A MX2022005573 A MX 2022005573A MX 2022005573 A MX2022005573 A MX 2022005573A MX 2022005573 A MX2022005573 A MX 2022005573A
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Mexico
Prior art keywords
event
relates
frugirperda
corn
methods
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MX2022005573A
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English (en)
Inventor
Carneiro Newton Portilho
Valicente Fernando Hercos
Carneiro Andréa Almeida
Noda Roberto Willians
Almeida Barros Beatriz De
Guimarães Claudia Teixeira
Oliveira Pinto Marcos De
Alves Meire De Cássia
Nobre Myriam Maia
Mendes Simone Martins
Parentoni Sidney Netto
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Empresa Brasileira De Pesquisa Agropecuaria Embrapa
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Publication of MX2022005573A publication Critical patent/MX2022005573A/es

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684Zea mays [maize]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/13Plant traits
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
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  • Botany (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La presente invención se refiere a un nuevo evento de maíz transgénico que expresa la proteína insecticida Cry1Da truncada o modificada designado evento ME240913. La invención informa los ácidos nucleicos que son únicos para el evento ME240913. También se definen, iniciadores, amplicón, métodos y kits para detectar la presencia del evento ME240913. La invención también se refiere a plantas de maíz que contienen el evento mencionado, usos de las mismas, métodos y composiciones de control de insectos plagas lepidópteros. La invención describe un evento de maíz que demostró un alto nivel de protección de plantas contra daños de alimentación producidos por Lepidoptera, incluyendo S. frugiperda, así como insectos resistentes a Cry1F. El evento de la invención se mostró altamente tóxico para S. frugiperda.
MX2022005573A 2019-11-06 2020-10-13 Molecula de acido nucleico del evento transgenico de maiz me240913 que expresa la proteina cry1da, celula, planta y semilla transgenica, usos de las mismas, producto de planta, metodo, kit y amplicon para la deteccion del evento, y metodos para producir una planta transgenica y de control de insectos plagas lepidopteros. MX2022005573A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BR102019023319-2A BR102019023319A2 (pt) 2019-11-06 2019-11-06 molécula de ácido nucléico do evento transgênico de milho me240913 expressando a proteína cry1da, célula, planta e semente transgênica, usos das mesmas, produto de planta, método, kit e amplicon para detecção do evento, e métodos para produzir uma planta transgênica e de controle de insetos-pragas lepidópteros
PCT/BR2020/050407 WO2021087586A1 (pt) 2019-11-06 2020-10-13 Molécula de ácido nucléico do evento transgênico de milho me240913 expressando a proteína cry1da, célula, planta e semente transgênica, usos das mesmas, produto de planta, método, kit e amplicon para detecção do evento, e métodos para produzir uma planta transgênica e de controle de insetos-pragas lepidópteros

Publications (1)

Publication Number Publication Date
MX2022005573A true MX2022005573A (es) 2022-06-09

Family

ID=75848005

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005573A MX2022005573A (es) 2019-11-06 2020-10-13 Molecula de acido nucleico del evento transgenico de maiz me240913 que expresa la proteina cry1da, celula, planta y semilla transgenica, usos de las mismas, producto de planta, metodo, kit y amplicon para la deteccion del evento, y metodos para producir una planta transgenica y de control de insectos plagas lepidopteros.

Country Status (6)

Country Link
US (1) US20230135492A1 (es)
CN (1) CN115968412A (es)
AR (1) AR120342A1 (es)
BR (1) BR102019023319A2 (es)
MX (1) MX2022005573A (es)
WO (1) WO2021087586A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116694627B (zh) * 2023-07-26 2023-09-29 隆平生物技术(海南)有限公司 转基因玉米事件lp035-1及其检测方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007107302A2 (en) * 2006-03-21 2007-09-27 Bayer Bioscience N.V. Novel genes encoding insecticidal proteins
AU2010330916B2 (en) * 2009-12-16 2015-07-16 Dow Agrosciences Llc Combined use of Cry1Da and Cry1Fa proteins for insect resistance management
AR099800A1 (es) * 2014-03-21 2016-08-17 Agrigenetics Inc Cry1d para controlar el gusano de la mazorca del maíz
UA123036C2 (uk) * 2014-10-16 2021-02-03 Монсанто Текнолоджі Ллс Сконструйований інсектицидний білок, який має активність проти лускокрилих
BR102018009263A8 (pt) * 2018-05-07 2023-01-31 Embrapa Pesquisa Agropecuaria Molécula de ácido nucleico cry1da códon-otimizada, construção de ácido nucleico, vetor, célula hospedeira, célula vegetal, planta transgênica, método para transformar uma célula, método para produzir uma planta transgênica, método de controle de pragas invertebradas de plantas de cultivo e usos da molécula de ácido nucleico

Also Published As

Publication number Publication date
AR120342A1 (es) 2022-02-09
WO2021087586A1 (pt) 2021-05-14
CN115968412A (zh) 2023-04-14
BR102019023319A2 (pt) 2021-05-18
US20230135492A1 (en) 2023-05-04

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