MX2021009762A - Metodo de purificacion. - Google Patents

Metodo de purificacion.

Info

Publication number
MX2021009762A
MX2021009762A MX2021009762A MX2021009762A MX2021009762A MX 2021009762 A MX2021009762 A MX 2021009762A MX 2021009762 A MX2021009762 A MX 2021009762A MX 2021009762 A MX2021009762 A MX 2021009762A MX 2021009762 A MX2021009762 A MX 2021009762A
Authority
MX
Mexico
Prior art keywords
porous region
sample
purification method
filter
nucleic acid
Prior art date
Application number
MX2021009762A
Other languages
English (en)
Inventor
Georgios Patsos
Original Assignee
Revolugen Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Revolugen Ltd filed Critical Revolugen Ltd
Publication of MX2021009762A publication Critical patent/MX2021009762A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B01D21/262—Separation of sediment aided by centrifugal force or centripetal force by using a centrifuge
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D35/00—Filtering devices having features not specifically covered by groups B01D24/00 - B01D33/00, or for applications not specifically covered by groups B01D24/00 - B01D33/00; Auxiliary devices for filtration; Filter housing constructions
    • B01D35/02—Filters adapted for location in special places, e.g. pipe-lines, pumps, stop-cocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/147—Microfiltration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/02—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor characterised by their properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/02—Inorganic material
    • B01D71/04—Glass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2325/00—Details relating to properties of membranes
    • B01D2325/04—Characteristic thickness
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/308—Adsorption or desorption
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

Abstract

La presente invención se refiere a un filtro para aislar ácido nucleico de una muestra y métodos para aislar y purificar ácido nucleico de una muestra. El filtro tiene una primera región porosa y una segunda región porosa. La primera región porosa está arreglada para ser contactada en uso por la muestra antes de la segunda región porosa, y la primera región porosa tiene un tamaño de poro nominal que es mayor que la segunda región porosa.
MX2021009762A 2019-02-15 2020-02-14 Metodo de purificacion. MX2021009762A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1902171.6A GB2581489B (en) 2019-02-15 2019-02-15 Purification method
PCT/GB2020/050348 WO2020165602A1 (en) 2019-02-15 2020-02-14 Purification method

Publications (1)

Publication Number Publication Date
MX2021009762A true MX2021009762A (es) 2021-11-17

Family

ID=65998630

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021009762A MX2021009762A (es) 2019-02-15 2020-02-14 Metodo de purificacion.

Country Status (16)

Country Link
US (1) US20220049243A1 (es)
EP (2) EP3985112A1 (es)
JP (1) JP2022520092A (es)
KR (1) KR20210128450A (es)
CN (2) CN113423832A (es)
AR (2) AR118098A1 (es)
AU (1) AU2020220830A1 (es)
BR (1) BR112021015952A2 (es)
CA (1) CA3130331A1 (es)
EA (1) EA202192261A1 (es)
GB (1) GB2581489B (es)
IL (2) IL287609B (es)
MX (1) MX2021009762A (es)
SG (1) SG11202108858UA (es)
TW (1) TW202045722A (es)
WO (1) WO2020165602A1 (es)

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4034036C2 (de) * 1990-10-26 1994-03-03 Diagen Inst Molekularbio Vorrichtung und Verfahren zur Isolierung von Nukleinsäuren aus Zellsuspensionen
DE4143639C2 (de) * 1991-12-02 2002-10-24 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren
KR20060027416A (ko) * 2001-07-09 2006-03-27 아사히 가세이 가부시키가이샤 부직포를 이용한 핵산 정제법 및 검출법
US20050026175A1 (en) * 2003-07-31 2005-02-03 John Link Devices and methods for isolating RNA
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
JP4690656B2 (ja) * 2004-02-12 2011-06-01 ジーエルサイエンス株式会社 核酸の分離精製方法及び分離吸着体
EP1626085A1 (en) * 2004-08-10 2006-02-15 Agilent Technologies, Inc. Devices and methods for isolating RNA
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
DE102005047736B4 (de) * 2005-09-29 2008-08-14 Aj Innuscreen Gmbh Verfahren und System zur Isolierung von Nukleinsäuren aus beliebigen komplexen Ausgangsmaterialien
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
CN101113437A (zh) * 2006-06-29 2008-01-30 米利波尔公司 自生物学样品中分离rna和dna
US7759112B2 (en) * 2007-10-31 2010-07-20 Akonni Biosystems, Inc. Apparatus, system, and method for purifying nucleic acids
US9428746B2 (en) * 2007-10-31 2016-08-30 Akonni Biosystems, Inc. Method and kit for purifying nucleic acids
CN102191226A (zh) * 2010-03-08 2011-09-21 北京清大天一科技有限公司 细胞培养混合物的分离纯化方法
JP6242896B2 (ja) * 2012-08-28 2017-12-06 アコーニ バイオシステムズ インコーポレイテッド 核酸を精製するための方法およびキット
EP4148141A1 (en) * 2014-02-10 2023-03-15 Zymo Research Corporation Kits for nucleic acid capture
ES2708205T3 (es) * 2014-04-11 2019-04-09 Fujifilm Wako Pure Chemical Corp Método de purificación de ácidos nucleicos
GB201504459D0 (en) * 2015-03-17 2015-04-29 Moorlodge Biotech Ventures Ltd Isolation of DNA
GB201609115D0 (en) * 2016-05-24 2016-07-06 Moorlodge Biotech Ventures Ltd Isolating nucleic acids

Also Published As

Publication number Publication date
BR112021015952A2 (pt) 2021-10-05
JP2022520092A (ja) 2022-03-28
AR122820A2 (es) 2022-10-05
US20220049243A1 (en) 2022-02-17
AU2020220830A1 (en) 2021-10-07
GB2581489A (en) 2020-08-26
IL287609A (en) 2021-12-01
IL285615B (en) 2022-08-01
EP3985112A1 (en) 2022-04-20
GB201902171D0 (en) 2019-04-03
SG11202108858UA (en) 2021-09-29
EP3924488A1 (en) 2021-12-22
IL287609B (en) 2022-07-01
AR118098A1 (es) 2021-09-15
CN114540343A (zh) 2022-05-27
CN113423832A (zh) 2021-09-21
EA202192261A1 (ru) 2021-12-21
GB2581489B (en) 2021-02-24
TW202045722A (zh) 2020-12-16
CA3130331A1 (en) 2020-08-20
WO2020165602A1 (en) 2020-08-20
IL285615A (en) 2021-09-30
KR20210128450A (ko) 2021-10-26

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