MX2020007805A - Sistema de lc-ms de columna dual y metodos para su uso. - Google Patents

Sistema de lc-ms de columna dual y metodos para su uso.

Info

Publication number
MX2020007805A
MX2020007805A MX2020007805A MX2020007805A MX2020007805A MX 2020007805 A MX2020007805 A MX 2020007805A MX 2020007805 A MX2020007805 A MX 2020007805A MX 2020007805 A MX2020007805 A MX 2020007805A MX 2020007805 A MX2020007805 A MX 2020007805A
Authority
MX
Mexico
Prior art keywords
dual
column
methods
monoclonal antibodies
disclosed method
Prior art date
Application number
MX2020007805A
Other languages
English (en)
Inventor
Shunhai Wang
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2020007805A publication Critical patent/MX2020007805A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/462—Flow patterns using more than one column with serial coupling of separation columns with different eluents or with eluents in different states
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G01N30/482—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se proporcionan métodos para lograr la cobertura de secuencia completa de anticuerpos monoclonales por digestión de tripsina y el sistema de LC-MS de columna dual. El método descrito mejora las técnicas actuales para el mapeo peptídico estándar.
MX2020007805A 2018-01-31 2019-01-29 Sistema de lc-ms de columna dual y metodos para su uso. MX2020007805A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862624352P 2018-01-31 2018-01-31
PCT/US2019/015542 WO2019152352A1 (en) 2018-01-31 2019-01-29 A dual-column lc-ms system and methods of use thereof

Publications (1)

Publication Number Publication Date
MX2020007805A true MX2020007805A (es) 2020-09-18

Family

ID=65529778

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020007805A MX2020007805A (es) 2018-01-31 2019-01-29 Sistema de lc-ms de columna dual y metodos para su uso.

Country Status (16)

Country Link
US (4) US10908166B2 (es)
EP (1) EP3746794A1 (es)
JP (1) JP2021513060A (es)
KR (1) KR20200115483A (es)
CN (1) CN111699392A (es)
AR (1) AR113730A1 (es)
AU (1) AU2019213643A1 (es)
BR (1) BR112020010978A2 (es)
CA (1) CA3084069A1 (es)
EA (1) EA202091825A1 (es)
IL (1) IL276100A (es)
MX (1) MX2020007805A (es)
SG (2) SG10202109446VA (es)
TW (2) TW202244494A (es)
WO (1) WO2019152352A1 (es)
ZA (1) ZA202003249B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10908166B2 (en) * 2018-01-31 2021-02-02 Regeneron Pharmaceuticals, Inc. Dual-column LC-MS system and methods of use thereof
JP2023501227A (ja) * 2019-10-29 2023-01-18 クアンタム－エスアイ インコーポレイテッド 配列決定のためにカートリッジを使用する方法およびデバイス

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3737516B2 (ja) 1995-06-26 2006-01-18 パーセプティブ バイオシステムズ，インコーポレーテッド 高速自動化連続流、多次元分子選別および分析
US5885841A (en) * 1996-09-11 1999-03-23 Eli Lilly And Company System and methods for qualitatively and quantitatively comparing complex admixtures using single ion chromatograms derived from spectroscopic analysis of such admixtures
JP2003534533A (ja) * 2000-02-08 2003-11-18 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン タンパク質のディファレンシャルディスプレイのマッピング
JP2001249113A (ja) * 2000-03-06 2001-09-14 Jasco Corp 高感度分離構造解析装置
EP1535053A2 (en) * 2002-08-16 2005-06-01 Geneprot, Inc. Multi-dimensional chromatography system for proteomics
JP2004101477A (ja) * 2002-09-12 2004-04-02 Yoshio Yamauchi ２次元高速液体クロマトグラフ装置及びそれを用いた蛋白質分析装置
EP1426442A1 (en) * 2002-12-02 2004-06-09 MTM Laboratories AG Marker molecules associated with colorectal lesions
WO2005000226A2 (en) * 2003-06-06 2005-01-06 Diversa Corporation Mixed bed multi-dimensional chromatography systems and methods of making and using them
WO2005073732A2 (en) * 2004-01-23 2005-08-11 Amgen Inc. Lc/ms method of analyzing high molecular weight proteins
CN101115540A (zh) * 2004-12-08 2008-01-30 谢佳辉 用于液相色谱的组合柱以及相关系统和方法
US20060157647A1 (en) * 2005-01-18 2006-07-20 Becton, Dickinson And Company Multidimensional liquid chromatography/spectrometry
US20060169640A1 (en) * 2005-02-01 2006-08-03 Hubert Quinn High throughput screening, purification and recovery system for large and small molecules
CN101042377A (zh) * 2006-03-20 2007-09-26 中国人民解放军军事医学科学院放射与辐射医学研究所 一种用于含组氨酸肽段富集与测序的方法和试剂盒
US7756646B2 (en) * 2006-03-31 2010-07-13 Battelle Memorial Institute Method for predicting peptide detection in mass spectrometry
US8017015B2 (en) * 2006-10-20 2011-09-13 Quest Diagnostics Investments Incorporated Monolithic column chromatography
WO2008128225A1 (en) * 2007-04-16 2008-10-23 Momenta Pharmaceuticals, Inc. Multi-dimensional chromatographic methods for separating n-glycans
US8074494B2 (en) * 2007-12-10 2011-12-13 Proxeon Biosystems A/S Plugged flow 2D chromatography
CN106461619A (zh) * 2014-02-11 2017-02-22 步琪公司 多柱色谱系统和使用的方法
CN105980847B (zh) * 2014-02-14 2018-12-04 通用电气健康护理生物科学股份公司 自动多步纯化系统
US10908166B2 (en) 2018-01-31 2021-02-02 Regeneron Pharmaceuticals, Inc. Dual-column LC-MS system and methods of use thereof
JP7194376B2 (ja) 2018-02-19 2022-12-22 マツダ株式会社 車両の制御装置

Also Published As

Publication number Publication date
KR20200115483A (ko) 2020-10-07
AU2019213643A1 (en) 2020-07-23
AR113730A1 (es) 2020-06-03
TW201940883A (zh) 2019-10-16
EP3746794A1 (en) 2020-12-09
IL276100A (en) 2020-08-31
TWI761660B (zh) 2022-04-21
BR112020010978A2 (pt) 2020-11-17
EA202091825A1 (ru) 2020-09-24
US20230393141A1 (en) 2023-12-07
JP2021513060A (ja) 2021-05-20
CA3084069A1 (en) 2019-08-08
CN111699392A (zh) 2020-09-22
US20190234960A1 (en) 2019-08-01
SG10202109446VA (en) 2021-10-28
US20220357336A1 (en) 2022-11-10
US20210109108A1 (en) 2021-04-15
WO2019152352A1 (en) 2019-08-08
US11740246B2 (en) 2023-08-29
ZA202003249B (en) 2023-10-25
US11435359B2 (en) 2022-09-06
SG11202004812VA (en) 2020-06-29
US10908166B2 (en) 2021-02-02
TW202244494A (zh) 2022-11-16

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