MX2016012260A - Resolubilizacion de cristales de proteina a bajo ph. - Google Patents

Resolubilizacion de cristales de proteina a bajo ph.

Info

Publication number
MX2016012260A
MX2016012260A MX2016012260A MX2016012260A MX2016012260A MX 2016012260 A MX2016012260 A MX 2016012260A MX 2016012260 A MX2016012260 A MX 2016012260A MX 2016012260 A MX2016012260 A MX 2016012260A MX 2016012260 A MX2016012260 A MX 2016012260A
Authority
MX
Mexico
Prior art keywords
low
resolubilization
protein
protein crystals
interest
Prior art date
Application number
MX2016012260A
Other languages
English (en)
Inventor
Peter Friis Esben
Erik Pedersen Poul
Martin Persson Jon
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2016012260A publication Critical patent/MX2016012260A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01017—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se describe un método para purificar un producto proteínico preparado en un proceso de fermentación donde la proteína de interés está presente en forma sólida, cristalina o amorfa en el contenedor de fermentación, donde el pH del caldo de fermentación se ajusta a un pH bajo, mediante lo cual se disuelve la proteína de interés y puede separarse de manera eficaz de la parte insoluble.
MX2016012260A 2014-03-28 2015-03-30 Resolubilizacion de cristales de proteina a bajo ph. MX2016012260A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14162436 2014-03-28
PCT/EP2015/056921 WO2015144936A1 (en) 2014-03-28 2015-03-30 Resolubilization of protein crystals at low ph

Publications (1)

Publication Number Publication Date
MX2016012260A true MX2016012260A (es) 2017-01-06

Family

ID=50396954

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016012260A MX2016012260A (es) 2014-03-28 2015-03-30 Resolubilizacion de cristales de proteina a bajo ph.

Country Status (7)

Country Link
US (1) US20170114091A1 (es)
EP (1) EP3122760A1 (es)
JP (2) JP6591998B2 (es)
CN (1) CN106132995A (es)
BR (1) BR112016022447A2 (es)
MX (1) MX2016012260A (es)
WO (1) WO2015144936A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017211803A1 (en) 2016-06-07 2017-12-14 Novozymes A/S Co-expression of heterologous polypeptides to increase yield
EP3606936B1 (en) * 2017-04-03 2021-10-20 Novozymes A/S Recovery process
WO2020058226A1 (en) * 2018-09-17 2020-03-26 Dsm Ip Assets B.V. Animal feed compositions and uses thereof
US20220025423A1 (en) 2018-11-28 2022-01-27 Novozymes A/S Modified Filamentous Fungal Host Cells
WO2021064068A1 (en) 2019-10-03 2021-04-08 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
CN116583534A (zh) 2020-11-02 2023-08-11 诺维信公司 前导肽和编码其的多核苷酸
CN113388532B (zh) * 2021-06-16 2023-04-07 华东理工大学 一种用于生产天冬酰胺酶的重组里氏木霉及其构建方法和应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP2002507426A (ja) * 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー アミノ酸置換を有するセリンプロテアーゼ変異体
AU2001254622A1 (en) * 2000-04-28 2001-11-12 Novozymes A/S Laccase mutants
WO2002018588A1 (en) * 2000-08-29 2002-03-07 Novozymes A/S Method for screening highly active proteases and inhibitors
DE60142646D1 (en) * 2000-11-22 2010-09-02 Univ Maryland Fusionprotein
CN1473932A (zh) * 2003-06-30 2004-02-11 中山大学 一种重组石斑鱼腺苷酸环化酶激活多肽基因及其表达系统、表达产物、生产方法和应用
CN1303207C (zh) * 2004-01-08 2007-03-07 中国药科大学 组织型纤溶酶原激活剂突变体的复性分离纯化方法
ES2368740T3 (es) * 2004-02-04 2011-11-21 Novo Nordisk A/S Métodos de replegamiento de glicosiltransferasas de mamífero.
US7381553B2 (en) * 2004-06-15 2008-06-03 The Board Of Trustees Of The Universtiy Of Illinois Highly active xylose reductase from Neurospora crassa
DK2125865T3 (da) * 2007-03-15 2014-10-06 Novozymes As Opløsning af proteasekrystaller i fermenteringssuppe
US8568714B2 (en) * 2008-05-23 2013-10-29 The United States Of America, As Represented By The Secretary Of Agriculture Lys K endolysin is synergistic with lysostaphin against MRSA
DK2510094T3 (en) * 2009-12-09 2017-03-13 Danisco Us Inc COMPOSITIONS AND METHODS OF COMPREHENSIVE PROTEASE VARIETIES
CN102234640B (zh) * 2010-04-22 2012-10-03 哈尔滨博翱生物医药技术开发有限公司 重组小分子泛素样修饰物蛋白酶及其制备方法和应用
CN102134575A (zh) * 2010-05-14 2011-07-27 昆明理工大学 大肠杆菌s-甲酰谷胱甘肽水解酶的原核表达载体及其构建方法和应用
DK2782592T3 (en) * 2011-11-25 2017-06-19 Novozymes As Polypeptides with lysozyme activity and polynucleotides encoding them
CN104114698A (zh) * 2012-02-17 2014-10-22 诺维信公司 枯草杆菌蛋白酶变体以及编码它们的多核苷酸
CA2871042A1 (en) * 2012-04-20 2013-10-24 The Sun Products Corporation Liquid detergent compositions
BR112015007466B1 (pt) * 2012-10-03 2022-10-11 Agrivida, Inc Protease inteína-modificada, cassete de expressão, hospedeiro, método de produção de uma protease e detergente

Also Published As

Publication number Publication date
BR112016022447A2 (pt) 2017-10-10
CN106132995A (zh) 2016-11-16
JP2019170384A (ja) 2019-10-10
EP3122760A1 (en) 2017-02-01
JP6591998B2 (ja) 2019-10-16
US20170114091A1 (en) 2017-04-27
JP2017508472A (ja) 2017-03-30
WO2015144936A1 (en) 2015-10-01

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