MX2015006813A - Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos. - Google Patents

Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos.

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Publication number
MX2015006813A
MX2015006813A MX2015006813A MX2015006813A MX2015006813A MX 2015006813 A MX2015006813 A MX 2015006813A MX 2015006813 A MX2015006813 A MX 2015006813A MX 2015006813 A MX2015006813 A MX 2015006813A MX 2015006813 A MX2015006813 A MX 2015006813A
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Mexico
Prior art keywords
cytochrome
activity
nanoparticles
recognised
enzyme
Prior art date
Application number
MX2015006813A
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English (en)
Inventor
Vázquez Duhalt Rafael
Paulina Sánchez Sánchez Lorena
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Univ Nac Autónoma De México
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Publication date
Application filed by Univ Nac Autónoma De México filed Critical Univ Nac Autónoma De México
Priority to MX2015006813A priority Critical patent/MX2015006813A/es
Priority to PCT/MX2016/000053 priority patent/WO2016195471A1/es
Priority to US15/577,404 priority patent/US10480012B2/en
Publication of MX2015006813A publication Critical patent/MX2015006813A/es

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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/86—Viral vectors
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    • A61K31/135—Amines having aromatic rings, e.g. ketamine, nortriptyline
    • A61K31/138—Aryloxyalkylamines, e.g. propranolol, tamoxifen, phenoxybenzamine
    • A—HUMAN NECESSITIES
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    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
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    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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    • A61K38/1732—Lectins
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    • A61K38/00—Medicinal preparations containing peptides
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    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/177—Receptors; Cell surface antigens; Cell surface determinants
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
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    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/44—Oxidoreductases (1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6901—Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
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    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
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    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
    • A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
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    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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Abstract

En la presente invención se diseñaron y sintetizaron proteínas de fusión con actividad citocromo P450 encapsuladas en una nanocápside (nanopartículas cargadas con actividad citocromo P450) y que son inmunológicamente inertes y que son reconocidas por células de cáncer de mama. La metodología consistió en el encapsulamiento de moléculas de citocromo P450 dentro de cápsides virales o nanopartículas de biopolímeros. En un caso, el gen que codifica la enzima citocromo P450 se fusiona con la secuencia nucleotídica de una versión truncada de la proteína de andamiaje del bacteriófago P22. Se expresan las proteínas heterologamente y las cápsides conteniendo en su interior el citocromo P450 son purificadas. Las nanopartículas enzimáticas se cubren con polietilenglicol dendrítico bifuncional y finalmente se funcionalizarán con el péptido cíclico Arg-Gly-Asp-D-Phe-Cys u otro ligando para ser reconocidas por la integrina avjß3/5 u otro receptor de las células tumorales de mama. Esta invención lleva específicamente a células tumorales la actividad citocromo P450 que activa más eficientemente y en el sitio requerido los profármacos usados en quimioterapia.
MX2015006813A 2015-05-29 2015-05-29 Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos. MX2015006813A (es)

Priority Applications (3)

Application Number Priority Date Filing Date Title
MX2015006813A MX2015006813A (es) 2015-05-29 2015-05-29 Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos.
PCT/MX2016/000053 WO2016195471A1 (es) 2015-05-29 2016-05-26 Nanopartículas biocatalíticas cyp-p22 con actividad citocromo p45o para la activación de profármacos
US15/577,404 US10480012B2 (en) 2015-05-29 2016-05-26 CYP-P22 biocatalytic nanoparticles with cytochrome P450 activity for prodrug activation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX2015006813A MX2015006813A (es) 2015-05-29 2015-05-29 Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos.

Publications (1)

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MX2015006813A true MX2015006813A (es) 2016-11-28

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MX2015006813A MX2015006813A (es) 2015-05-29 2015-05-29 Nanoparticulas biocataliticas cyp-p22 con actividad citocromo p450 para la activacion de profarmacos.

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US (1) US10480012B2 (es)
MX (1) MX2015006813A (es)
WO (1) WO2016195471A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3600261A4 (en) * 2017-03-21 2020-10-21 Pharmacyte Biotech, Inc. CYTOCHROME P450-PRODUCING ENCAPSULATED CELLS AND METHODS OF USE

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688773A (en) * 1994-08-17 1997-11-18 The General Hospital Corporation Method of selectively destroying neoplastic cells
RU2223788C2 (ru) 1996-03-27 2004-02-20 Бавариан Нордик Рисерч Инститьют А/С Трансдуцирующие цитохром p450 ретровирусные векторы
AU3266899A (en) 1998-03-06 1999-09-20 Oxford Biomedica (Uk) Limited Enhanced prodrug activation

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WO2016195471A1 (es) 2016-12-08
US10480012B2 (en) 2019-11-19

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