MX2013014195A - Dispositivos y metodos para la captura eficiente de acidos nucleicos. - Google Patents
Dispositivos y metodos para la captura eficiente de acidos nucleicos.Info
- Publication number
- MX2013014195A MX2013014195A MX2013014195A MX2013014195A MX2013014195A MX 2013014195 A MX2013014195 A MX 2013014195A MX 2013014195 A MX2013014195 A MX 2013014195A MX 2013014195 A MX2013014195 A MX 2013014195A MX 2013014195 A MX2013014195 A MX 2013014195A
- Authority
- MX
- Mexico
- Prior art keywords
- microarray
- capture
- zone
- molecules
- target molecules
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 238000002493 microarray Methods 0.000 abstract 6
- 230000003993 interaction Effects 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000010195 expression analysis Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000007899 nucleic acid hybridization Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000012163 sequencing technique Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La presente invención se relaciona con un dispositivo para la unión eficiente de ácidos nucleicos en una micromatriz, que comprende una zona de reacción que comprende una micromatriz, y una zona de captura que comprende un sustrato de membrana porosa, en donde la zona de captura es capaz de capturar específicamente hebras sentido de moléculas diana, mientras que hebras antisentido complementarias son capturadas en la zona de reacción, o viceversa. La invención contempla además unidades reguladoras de temperatura en el dispositivo que permiten llevar o mantener la captura a una temperatura que no permite la hibridación o unión de ácidos nucleicos a las moléculas de captura o a una temperatura adecuada para la hibridación de ácidos nucleicos. La invención también se relaciona con un método de unir eficientemente ácidos nucleicos sobre una micromatriz, que comprende introducir un medio que contiene una o más moléculas diana en forma desnaturalizada en una zona de captura de un dispositivo de la presente invención, llevar a cabo una reacción de interacción entre las moléculas diana y moléculas de captura inmovilizadas en la zona de captura, y transportar hebras de moléculas diana no unidas a una zona de reacción que comprende una micromatriz, permitiendo así una interacción entre las hebras de moléculas diana y moléculas de captura inmovilizadas sobre la micromatriz. En un aspecto adicional la invención se relaciona con el uso del dispositivo para enriquecer moléculas diana, seleccionando específicamente moléculas diana, para análisis de expresión, hibridación genómica comparativa, la detección de SNPs, o para la secuenciación basada en selección genómica basada en micromatriz.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11168864A EP2532754A1 (en) | 2011-06-07 | 2011-06-07 | Devices and methods for efficient capture of nucleic acids |
| PCT/IB2012/052578 WO2012168812A1 (en) | 2011-06-07 | 2012-05-23 | Devices and methods for efficient capture of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2013014195A true MX2013014195A (es) | 2014-03-21 |
Family
ID=44773862
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013014195A MX2013014195A (es) | 2011-06-07 | 2012-05-23 | Dispositivos y metodos para la captura eficiente de acidos nucleicos. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20140087955A1 (es) |
| EP (2) | EP2532754A1 (es) |
| JP (1) | JP2014518639A (es) |
| CN (1) | CN103703144A (es) |
| MX (1) | MX2013014195A (es) |
| WO (1) | WO2012168812A1 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017152357A1 (en) * | 2016-03-08 | 2017-09-14 | Coyote Bioscience Co., Ltd. | Methods and systems for analyzing nucleic acids |
| CN109689887A (zh) * | 2016-07-08 | 2019-04-26 | 加州理工学院 | 用于进行低浓度分析物的流动通过捕获的方法和装置 |
| AU2019231794A1 (en) * | 2018-03-08 | 2020-10-29 | Pathogendx, Inc. | Microarray based multiplex pathogen analysis and uses thereof |
| CN110452970B (zh) * | 2019-08-13 | 2023-08-18 | 珠海澳加动力生物科技有限公司 | 一种基因融合的微流控检测设备及其使用方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9622665D0 (en) * | 1996-10-31 | 1997-01-08 | Zeneca Ltd | Methods |
| WO2002095065A2 (en) * | 2001-05-18 | 2002-11-28 | Azign Bioscience A/S | G-protein coupled receptor arrays |
| CN101528351A (zh) * | 2006-10-17 | 2009-09-09 | 皇家飞利浦电子股份有限公司 | 用于扩增和检测核酸的装置 |
| US20100093986A1 (en) | 2007-02-02 | 2010-04-15 | Zwick Michael E | Methods of direct genomic selection using high density oligonucleotide microarrays |
| EP2103352A1 (en) * | 2008-03-18 | 2009-09-23 | Koninklijke Philips Electronics N.V. | Membranes suited for immobilizing biomolecules |
| CN107419002A (zh) * | 2009-09-01 | 2017-12-01 | 皇家飞利浦电子股份有限公司 | 用于微阵列选择的设备和方法 |
| US20110086772A1 (en) * | 2009-09-25 | 2011-04-14 | Signature Genomics Laboratories Llc | Multiplex (+/-) stranded arrays and assays for detecting chromosomal abnormalities associated with cancer and other diseases |
-
2011
- 2011-06-07 EP EP11168864A patent/EP2532754A1/en not_active Ceased
-
2012
- 2012-05-23 JP JP2014514169A patent/JP2014518639A/ja not_active Withdrawn
- 2012-05-23 WO PCT/IB2012/052578 patent/WO2012168812A1/en active Application Filing
- 2012-05-23 EP EP12725524.8A patent/EP2718460A1/en not_active Withdrawn
- 2012-05-23 CN CN201280028119.9A patent/CN103703144A/zh active Pending
- 2012-05-23 MX MX2013014195A patent/MX2013014195A/es unknown
- 2012-05-23 US US14/118,986 patent/US20140087955A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012168812A1 (en) | 2012-12-13 |
| US20140087955A1 (en) | 2014-03-27 |
| CN103703144A (zh) | 2014-04-02 |
| EP2532754A1 (en) | 2012-12-12 |
| EP2718460A1 (en) | 2014-04-16 |
| JP2014518639A (ja) | 2014-08-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shendure et al. | Next-generation DNA sequencing | |
| NZ610129A (en) | Systems and methods for automated reusable parallel biological reactions | |
| JP2014204730A5 (es) | ||
| WO2008115185A3 (en) | Use of microarrays for genomic representation selection | |
| EP2044191A4 (en) | SYSTEMS AND METHOD FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES | |
| WO2011027268A3 (en) | Devices and methods for microarray selection | |
| JP2015156872A5 (es) | ||
| WO2007146819A3 (en) | Methods for identifying and using snp panels | |
| WO2014015084A3 (en) | System and methods for detecting genetic variation | |
| RU2013113407A (ru) | Способы улавливания и секвенирования нуклеиновых кислот | |
| WO2010026488A3 (en) | Methods and kits for nucleic acid sequencing | |
| Karamitros et al. | Multiplexed targeted sequencing for oxford nanopore MinION: a detailed library preparation procedure | |
| MX2013014195A (es) | Dispositivos y metodos para la captura eficiente de acidos nucleicos. | |
| JP2014518638A5 (es) | ||
| JP2011101652A5 (es) | ||
| NZ588112A (en) | Expression-linked gene discovery | |
| AU2009355009A8 (en) | THD primer target detection | |
| WO2009143500A3 (en) | Methods for removing nucleic acid contamination from reagents | |
| WO2018148463A1 (en) | Nucleic acid hybridization assay | |
| DK2162549T3 (da) | Fremgangsmåde til at detektere nukleinsyrer | |
| Igartua et al. | Targeted enrichment of specific regions in the human genome by array hybridization | |
| CN102827830A (zh) | 一种捕获核酸片段的方法 | |
| CA2797319A1 (en) | A new combination of eight risk alleles associated with autism | |
| CN104560950A (zh) | 一种基于mda的全基因组扩增的方法 | |
| WO2011116120A3 (en) | Method and apparatus for addressable flow cells in single molecule sequencing |