MX2012009454A - Media, kits, systems and methods for the micropropagation of bamboo. - Google Patents

Abstract

Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.

Description

MEANS, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF THE BAMBU Field of the Invention Means, kits, systems and methods for the micropropagation of bamboo are described herein.

Background of the Invention The subfamily Bambusoideae (of the family Poaceae), includes woody and herbaceous bamboos. At present, approximately 120 genera of temperate and tropical woody bamboos are recognized. Bamboos are versatile plants with many different applications. It has been estimated that approximately 2.2 billion people worldwide use bamboo to some degree, and in 1985 the global income attributable to bamboo was estimated at approximately $ 4.5 billion. The market for bamboo has also expanded. Bamboo shoots are a staple in Asian cuisine, and bamboo is found in a number of products that include toothpicks, brooms, poles for viticulture and arboriculture, landscaping materials, parquet flooring, laminate materials, furniture , crafts and other household items. In addition, bamboo has become an important source of textile material as a component of paper production and as a source of structural wood.

Bamboo is considered an "ecological" product that does not harm the environment. One of the characteristics that gives bamboo its ecological reputation is its extremely rapid growth rate. Bamboo is the fastest growing woody plant in the world, reaching growth rates of over 91.44 cm (three feet) per day. It reaches this growth rate partly due to its system of rhizomes, which is able to provide much of the energy towards the growth of the shoot.

Despite the rapid growth rate of bamboo, it has other characteristics that make it a difficult crop to control. Perhaps the greatest difficulty comes from the fact that many commercially important bamboos develop-only at intervals as long as 60-130 years. The factor that aggravates the difficulties of this long cycle of development is the fact that many bamboos exhibit a massive (or gregarious) development, in which all plants in the population develop simultaneously. For example, Phyllostachys bambusoides develops at an interval of 130 years, and in this species all plants of the same species develop at the same time, regardless of differences in geographical location or climatic conditions. After the growth, the bamboo dies.

The very long growth interval and the risk of the bamboo developing massively makes it very difficult to obtain the seeds for propagation. The complicating factor of this problem is the fact that bamboo seeds, even when available, are only viable for no more than 3-6 months.

As a result of these difficulties with the propagation of bamboo by seed, bamboo is commonly propagated by asexual techniques such as division and cutting of groups. These asexual propagation techniques, however, are insufficient to meet the projected world demand because their capacity to generate mass scale production, and their practical effectiveness, are too low. In addition many asexual propagation methods have the disadvantage of not being able to eliminate the pathogens present in the plants of origin.

A method to achieve large-scale production of bamboo is very desirable. Micropropagation (also known as tissue culture with the terms alternatively used herein), is an excellent method to achieve this goal.

Micropropagation is not different from the development of plants from cuts. However, contrary to the development of plants from cuts, micropropagated plants are developed in vitro in sterile media. Commonly, the media comprises agar, with the addition of various compounds such as nutrients, inorganic salts, growth regulators, sugars, vitamins and other compounds.

An advantage of tissue culture plants is that they can be grown in a sterile environment so that they remain disease-free. Other advantages include the ability to develop a large number of plants in a small space, with the reduced water and nutrients that are necessary for micropropagated plants, and the rapid multiplication of tissues that in turn can be used to produce more material from tissue culture. On the other hand, micropropagation is very flexible and rapid increase is possible (in a period of 1 year almost one million plants can be produced from any genotype). Such short periods and quantities can not be overcome by any conventional method. The tissue culture also provides the production of high quality plants that are easy to transport and ship.

Some documents dealing with the cultivation of bamboo weaving have been published. In practice, however (ie, for the propagation of bamboos on a large or mass scale), the methods described in these documents are not implied in commercially viable propagation systems.

The difficulties encountered in the cultivation of bamboo tissue are high incidences of endogenous or superficial contamination and decomposition, the factors related to inactivity or topófisis and hyperhydricity. The present disclosure provides means, systems and methods that overcome these difficulties by allowing commercial asexual production of bamboo.

Brief Description of the Invention The present description overcomes the difficulties encountered in the commercial asexual production of bamboo by providing efficient means, systems and methods for growing bamboo tissue.

One embodiment described herein is a means for micropropagation of bamboo wherein the medium comprises meta-topolin or thidiazuron. In another embodiment, the medium comprises meta-topolin and thidiazuron. In another modality, the medium is medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2- M, medium CW2-i¡¡, medium CW2-¡v, medium CW2-v, medium b-10-¡, medium of b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b- 11 -i, medium b-11-ii, medium b-11-iii, medium of b-11-iv, medium b-11-v, medium b-12c-i, medium b-12c-¡, medium b - 12 c- iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b - 1 - ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b - 4 - i, medium b-4-ii, medium b-4-üi, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6-, medium CW1-Í, medium CW-1-ii, medium CW1-iii , CW1-iv medium, CW1-V medium, CW3-i medium, CW3-U medium, CW3-iii medium, CW3-iv medium, mean CW3- v, medium CW4-I, medium CW4-Ü, medium CW4-ÍÜ, medium CW4-iv, medium CW4-V, medium CW5-Ì, medium CWS-ii, medium CW5-ÜÍ, medium CW5-iv, medium CW5-v, medium CW6-Í, medium CW6-Ü, medium CW6-ÜÍ, medium CW6-iv and / or CW6-medium.

The embodiments described herein also include systems for micropropagation of bamboo. In one embodiment, the system comprises a kit comprising a medium comprising meta-topolin or thidiazuron. In another embodiment, meta-topolin and thidiazuron are found in the same medium or in a separate medium. In another modality, the medium is medium dé b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2 -ÍÍ, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b- 10 - v, medium of b-11-i, medium b-11-ii, medium b-11-iii, medium b-11- iv, medium b-11- ?, medium b-12c-i, medium b-12c -ii, medium of b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b- 1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b-6 -i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-i, medium of CW1-Ü, medium CW1-iii, medium CW1- iv, medium CW1-v, medium CW3-Í, medium CW3-Ü, medium CW3-¡¡¡, medium CW3-ÍV, medium CW3-V, medium CW4-Í, medium CW4-Ü, medium CW4-ÜÍ, medium CW4-iv, medium CW4-v, medium CW5-Í, medium CW5-Ü, medium CW5-Ü, medium CW5-iv, medium CW5-V, medium CW6-,, medium CW6-Ü, medium CW6-Ü, medium CW6-iv and / or CW6-V medium.

The modalities described in the present also include methods for the micropropagation of bamboo. In one embodiment, the method comprises culturing bamboo explants, cultures and / or shoots in the medium comprising meta-topolin and / or thidiazuron. In another embodiment, meta-topolin and thidiazuron are found in the same medium or in a separate medium. In another modality, the medium is medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-¡, medium CW2- U, medium CW2-iii, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10 - ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c -ii, medium b-12c-i¡¡, medium b-12c-v, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b -1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b- 6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-Ü, medium CW1-ii¡, medium CW1 -iv, medium CW1-V, medium CW3-Ì, medium CW3-Ü, medium CW3-ÜÍ, medium CW3-iv, medium CW3-v, medium CW4-Ì, medium CW4-Ü, medium CW4-iii, medium CW4 -iv, medium CW4-V, medium CW5-Í, medium CW5-Ü, medium CW5-ÍÜ, medium CW5-iv, medium CW5-v, medium CW6-,, medium CW6-Ü, medium CW6-Ü, medium CW6-iv and / or CW6-v medium.

The modalities include, without limitation: 1. A means comprising, consists essentially of or consists of: or 2. A method for the micropropagation of bamboo that involves exposing a bamboo explant to a mode 1 medium. 3. A method according to mode 2 which additionally comprises exposing a bamboo shoot to a medium b-9-i, medium b-9-ii, medium b-9-iü, medium b-9-iv, medium b-9 - ?, medium CW2-i, medium CW2-Ü, medium CW2-i¡¡, medium CW2-ÍV, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii , medium b-10-iv, medium b-10- ?, medium b- 1 -i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i. medium b-12c-ii, medium b- 12 ci ii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b -6-i, medium b-6-ii, medium b-6-iü, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-ii, medium CW1-iii, medium CW1 -iv, medium CW1-V, medium CW3-Í, medium CW3-Ü, medium CW3-iii, medium CW3-iv, medium CW3- v medium CW4-Í, medium CW 4 - ii, medium CW4-ÜÍ, medium CW4-iv medium CW4-v, medium CW5-Í, medium CW5 -Ü, medium CW5-iii medium CW5-VV, medium CW5-V, medium CW6-,, medium CW6-Ü medium CW6-iii, medium CW6-iv and / or medium CW6-V. 4. A method of. according to modality 3, which additionally comprises exposing a bamboo shoot to the medium that supports the transition to ex vitro conditions. 5. A method according to mode 3, where the method produces 100,000 bamboo shoots from an explant. 6. A method according to any of embodiments 2, 3, 4 or 5, wherein the method further comprises obtaining an explant. 7. A method according to mode 6, where the explant is the third node of the base of a bamboo cane. 8. A kit comprising a means consisting of: 9. A kit according to the modality 8, which additionally comprises a medium b-9-i, medium b-9-ii, medium b-9-iü, medium b-9-iv, medium b-9- ?, medium CW2 -i, medium CW2-Ü, medium CW2-ÍU, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv , medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-¡, medium b-12c-ii, medium b-12c-iii, medium b-12c-v, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-ii , medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4? medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6-, medium CW1-Í, medium CW1-Ü, medium CW1 -iii, medium CW1-iv, medium CW1-V, medium CW3-i, medium CW 3 -i, medium CW3-iii, medium CW3-iv, medium CW3-V, medium CW4-i, medium CW4-Ü, medium CW4-iii, medium CW4-iv, medium CW4-V, medium CW5-I, medium CW5-Ü, medium CW5-iii, medium CW5-iv, medium CW5-V, medium CW6-i, medium CW6-Ü , CW6-iii medium, CW6-medium and CW6-V medium. 10. A kit according to the modality 8 or 9, which additionally comprises a medium that supports the transition to ex vitro conditions. 11. A kit according to the modality 8, 9, or 10, which additionally comprises an explant. 12. A method for bamboo micropropagation, comprising exposing a bamboo explant to a medium comprising meta-topolin and / or thidiazuron. 13. A method according to the modality-12, where a medium comprises medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-i, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b- 10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c -i, medium b-12c-ii, medium b-12c-¡¡¡, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b- 1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4 - ?, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6-, medium CW1-¡, medium CW1-Ü, medium CW1 -iii, medium CW1-iv, medium CW1-V, medium CW3-Í, medium CW3-M, medium CW3-iii, medium CW3-iv, medium CW3-V, medium CW4-i, medium GW4-Ü, medium CW4-iii, medium CW4-iv, medium CW4-V, medium CW5-Í, medium CW5-Ü, medium / CW5-iii, medium CW5-iv, medium CW5-V, medium CW6-i, medium CW6-ii, medium CW6-iii, medium CW6-ÍV and / or CW6-V medium. 14. A method according to the modality 12, where the explant is the third node of the base of a bamboo cane. 15. A medium comprising a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-ii, medium b - 12 c - iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1- ?, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-v, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-i, medium CW1-Ü, medium CW -iii, medium CW1-ÍV, medium CW1- V, medium CW3-i, medium CW3-Ü, medium CW3-ÜÍ, medium CW3-iv, medium CW3-V, medium CW4-i, medium CW4-ii, medium CW4-Ü, medium CW4-iv, medium CW4- V, medium CW5-,, medium CW5-Ü, medium CW5-ii¡, medium CW5-iv, medium CW5-v, medium CW6-,, medium CW6-Ü, medium CW6-ii¡, medium CW6-iv and / or half CW6-v. 16. A medium consisting essentially of a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?. mean CW2-I, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10 -iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b - 1 - i, medium b - 11 - ?, medium b - 12c - i, medium b - 12c - ¡, medium b - 12c - iii, medium b - 12c - iv, medium b - 12c - v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b -4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b- 6- ?, medium CW1-i, medium CW1-Ü, medium CW1-iii, medium CW1-iv, medium CW1-v, medium CW3-i, medium CW3-Ü, medium CW3-iii, medium C 3-iv , CW3-V medium, CW4-i medium, CW4-Ü medium, CW4-iii medium, CW4-iv medium, CW4-v medium, CW5-medium, CW5-Ü medium, CW5-ii medium, CW5 medium i V. CW5-V medium, CW6-I medium, CW6-U medium, CW6-iii medium, CW6-iv medium and / or CW6-V medium. 17. A medium consisting of a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-i, medium CW2-Ü , medium CW2-iii, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10-? , medium b- 1 -i. medium b-11-ii, medium b-11-iü, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-ii, medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1 - i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1? medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-i, medium CW1-Ü, medium CW1-¡¡¡, medium CW1-iv, medium CW1-v, medium CW3- Í, medium CW3-Ü, medium CW3-ÍÜ, medium CW3-ÍV, medium CW3-V, medium CW4-i, medium CW4-ii, medium CW4-¡¡¡, medium CW4-iv, medium CW4-v, medium CW5-Í, medium CW5-Ü, medium CW5-iii, medium CW5-iv, medium CW5-V, medium CW6-Í, medium CW6-ii, medium CW6-iii, medium CW6-iv and / or CW6-V medium. 18. Kit comprising a medium according to the modality 15, 16 or 17. 19. A method of micropropagation of bamboo that uses a medium according to the modality 1, 15, 16 or 17. 20. A bamboo micropropagation method that uses a kit according to the modality 8, 9, 10, 11 or 18.

Additional modalities include: 21. A means comprising, consists essentially of or consists of: wherein the cytokinin A is meta-topolin or a meta-topolin analog as described herein and the cytokinin B is thidiazuron or a thidiazuron analogue as described herein. 22. A method for the micropropagation of bamboo, which involves exposing a bamboo explant to a mode 21 medium. 23. A method according to mode 22, which additionally comprises exposing a bamboo shoot to a medium b-9-i, medium b-9-ii, medium b-9-iii,. medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2-M, medium CW2-i¡¡, medium CW2-iv, medium CW2-v, medium b-10-i, medium b -10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11- ?, medium b-11-ii.i, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-ii, medium b-12c-iii, medium b-12c-v, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b -4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-i, medium b-6-iii, medium b-6-iv, medium b -6- ?, medium CW1-Ì, medium CW1-Ü, medium CW1-ÌM, medium CW1-iv, medium CW1-v, medium CW3-i, medium CW3-Ü, medium CW3-ÜÍ, medium CW3-iv, medium CW3-V, medium CW4-,, medium CW4-Ü, medium CW4-ii¡, medium CW4-iv, medium CW4-v, medium CW5-,, medium CW5-Ü, medium CW5-iii, medium CW5-iv , medium CW5-V, medium CW6-i, medium CW6-Ü, medium CW6-ÜÍ, medium CW6-iv and / or CW6-V medium. 24. A method according to mode 23, which additionally comprises exposing a bamboo shoot to a medium that supports the transition to ex vitro conditions. 25. A method according to mode 23, where the method produces 100,000 shoots of bamboo from an explant. 26. A method according to any of the embodiments 22, 23, .24 or 25, wherein the method further comprises comprising obtaining an explant. 27. A method according to mode 26, where the explant is the third node of the base of a bamboo cane. 28. A kit comprising a means comprising, consists essentially of or consists of: wherein the cytokinin A is meta-topolin or a meta-topolin analog as described herein and the cytokinin B is thidiazuron or a thidiazuron analogue as described herein. 29. A kit according to the modality 28, which additionally comprises a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2 -I, medium CW2-U, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-¡, medium b-10-ii, medium b - 10 - iii, medium b-10-iv , medium b-10- ?, medium b-11-i, medjo b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-U, medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b -6-i, medium b-6- ?, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-Ü, medium CWI-iii, medium CW1 -iv, medium CW1-v, medium CW3-Ì, medium CW3-Ü, medium CW3-iii, medium CW3-iv, medium CW3-v, medium CW4-Ì, medium CW4-Ü, medium CW4-ÜÍ, medium CW4 -iv, CW4-v medium, CW5-i medium, CW5-Ü medium, CW5-ÍM medium, CW5-iv medium, CW5-v medium, CW6-i medium, CW6-Ü medium, CW6-iii medium, CW6 medium -iv and / or CW6-v medium . 30. A kit according to mode 28 or 29, which additionally comprises a medium that supports the transition to ex vitro conditions. 31. A kit according to the modality 28, 29, or 30, which additionally comprises an explant. 32. A method for bamboo micropropagation, comprising exposing a bamboo explant to a medium comprising meta-topolin or a meta-topolin analog as described herein and / or thidiazuron or a thidiazuron analogue as described in I presented. 33. A method according to the modality 32, where the medium comprises a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, mean CW2-i, medium CW2-ii, medium CW2-ÍÜ, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10-üi, medium b-10 -iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c- i, medium b-12c-ii, medium b-12c-üi, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-ii , Medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-? , medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-i, medium CW1-N, medium CW -iii , medium CW1-ív, medium CW1-v, medium CW3-Í, medium CW3-Ü, medium CW3-iii, medium CW3-iv, medium CW3-V, medium CW4-Í, medium CW4-Ü, medium CW4-iii , medium CW4-iv, medium CW4-V, medium CW5-I, medium CW5-Ü, medium CW5-i¡¡, medium CW5-iv, medium CW5-V, medium CW6-i, medium CW6- i, CW6-iii medium, CW6-iv medium and / or CW6-V medium. 34. A method according to the modality 32, where the explant is the third node of the base of a bamboo cane. 35. A medium comprising a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2-Ü, medium CW2-ÜÍ, medium CW2-¡v, medium CW2-V, medium b-10-i, medium b-10-¡, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-i ¡, Medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv , medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-Ü, medium CW1-iii, medium CW1-iv, medium CW1 -V, CW3-i medium, CW3-Ü medium, CW3-iii medium, CW3-iv medium, CW3-V medium, CW4-i medium, CW4-ii medium, CW4-iii medium, CW4-iv medium, CW4 medium -V, medium CW5-i, medium CW5-Ü, medium CW5-iii, medium CW5-iv, medium CW5-v, medium CW6-i, medium CW6-Ü, medium CW6-iii, medium CW6-iv and / or half CW6-v. 36. A medium consisting essentially of a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-i, medium CW2- Ü, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-v, medium b-11-, medium b-12c-i, medium b-12c- ii, medium b-12c- ü, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1- iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-ii, medium b-4-iv-, medium b-4-, medium b-6 -i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-Ü. mean CW1-iii, CWI-iv medium, CW1-v medium, CW3-i medium, CW3-Ü medium, CW3-iii medium, CW3-iv medium, CW3-v medium, CW4-i medium, CW4-Ü medium , medium CW4-iii, medium CW4-iv, medium CW4-v, medium CW5-i, medium CW5-U, medium CW5-Ü, medium CW5-iv, medium CW5-v, medium CW6-i, medium CW6-U , CW6-iii medium, CW6-iv medium and / or CW6-V medium. 37. A medium consisting of a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2-Ü , medium CW2-ÍÜ, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10- iii, medium b-10-iv, medium b-10-? , medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12 ci i, medium b - 12 c- iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1- ?, medium b-4-i, medium b-4-, medium b-4-ii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-M, medium CW 1 -iii, medium CW1-iv, medium CW1-v, CW3-i medium, CW3-Ü medium, CW3-Ü medium, CW3-iv medium, CW3-V medium, CW4-i medium, CW4-ii medium, CW4-ii medium, CW4-iv medium, mean CW4-v, medium CW5-i, medium CW5-Ü, medium CW5-iii, medium CW5-iv, medium CW5-V, medium CW6-i, medium CW6-Ü, medium CW6-üi, medium CW6-iv and / or medium CW6-V. 38. Kit comprising a medium according to the modality 35, 36 or 37. 39. A method for the micropropagation of bamboo using a medium according to the modality 21, 35, 36 or 37. 40. A method for the micropropagation of bamboo using a kit according to the modality 28, 29, 30, 31 or 38. 41. A medium for bamboo micropropagation where the medium supports 10-120 cycles of multiplication per day for at least 1 month, at least 3 months, at least 6 months, at least 9 months, at least 12 months , at least 15 months, at least 18 months, at least 21 months, at least 24 months or at least 36 months. 42. A medium according to the embodiment 41, wherein the medium comprises, consists essentially of or consists of a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-¡, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10 -iii, medium b-10-iv, medium b-10-, medium b-11-, medium b- 11 - ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12 c-ii, medium b-12c-¡ii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1 -ii, medium b-1-üi, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4- iv, medium b-4-, medium b-6-i, medium b-6-i'i, medium b-6-iü, medium b-6-iv, medium b-6-, medium CW1-Í , medium CW1-Ü, medium CWI-iii, medium CW1-iv, medium CW1-v, medium CW3- i, CW3-Ü medium, CW3-iii medium, CW3-iv medium, CW3-V medium, CW4-medio medium, CW4-Ü medium, CW4-¡medium, CW4-iv medium, CW4-V medium, medium CW5-i, medium CW5-ÌI, medium CW5-iii, medium CW5-iv, medium CW5-V, medium CW6-Ì, medium CW6-Ü, medium CW6-iii, medium CW6-iv and / or CW6-v medium .

Additional modalities include: 43. A means for micropropagation of bamboo where the medium comprises meta-topolin or an analogue thereof and supports 10-120 cycles of multiplication per day for at least six months. 44. A medium according to mode 43, wherein the medium supports 10-120 multiplication cycles per day for at least one year. 45. A medium according to mode 43 or 44, wherein the meta-topolin or analogue thereof is present in an amount of 0.0125 mg / ml-10 mg / ml. 46. A medium according to mode 43, 44, or 45, wherein the medium additionally comprises thidiazurone or an analogue thereof. 47. A means according to the mode 43, 44, 45, or 45, wherein the means additionally comprises NAA, BAP, 2ip and / or IBA. 48. A method for the micropropagation of bamboo, which comprises cultivating bamboo explants and / or shoots in a medium of mode 43, 44, 45, 46 or 47. 49. A method for the micropropagation of bamboo according to the modality 48, where the bamboo is Phyllostachys bissetti; Fargesia denudata; Pleioblastus fortunei; Sasa Veilchii; Pleioblastus virldistriatus; Thamnocalamus crassinodus; Chusquea Culeo "Cana Prieta"; Bambusa Old Hamii; Phyllostachys Moso; Phyllostachys Atrovaginata; Dendrocalamus Asper; or Guadua Angustifolia. 50. A means for transferring the suckers to ex vitro conditions, wherein the medium comprises, consists essentially of or consists of a medium of Br-2-i, medium of Br-2-ii, medium of Br-2-iii, medium of Br-2-iv, Br-2- medium, Ech-i medium, Ech-ii medium, Ech-iii medium, Ech-iv medium, Ech-v medium, Amel-i medium, half of Amel-ii, half of Amel-iii, half of Amel-iv or half of Amel-v. 51. A means for micropropagation of bamboo, wherein the medium comprises thidiazuron or an analogue thereof and supports 10-120 cycles of multiplication per day for at least six months. 52. A medium according to the mode 51, wherein the medium supports 10-120 multiplication cycles per day for at least one year. 53. A medium according to mode 51 or 52, wherein the thidiazuron or analogue thereof is present in an amount of 0.0001 mg / ml-5 mg / ml. 54. A medium according to the mode 51, 52, or 53, wherein the medium additionally comprises meta-topolin or an analogue thereof, NAA, BAP, 2 i p and / or IBA. 55. A medium according to the mode 54, wherein the meta-topolin or analogue thereof is present in an amount of 0.0125 mg / ml-10 mg / ml. 56. A medium according to the mode 43, 44, 45, 46, 47, 51, 52, 53, 54 or 55, wherein the medium comprises, consists essentially of or consist of a medium b-9-i, medium b- 9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-i, medium CW2-Ü, medium CW2-¡¡¡, medium CW2-iv, medium CW2- V, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii , medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b-12c-ii, medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b-1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6- ?, medium b-6-i¡¡, medium b-6-iv, medium b-6- ?, medium CW1-Í, medium CW1-M, medium CW1-ii¡, medium CW1-iv, medium CW1-V, medium CW3-i, medium CW3-Ü, medium CW3-ÜÍ, medium CW3-v, medium CW3-V, medium CW4-,, medium CW4-,, medium CW4-¡, medium CW4-iv, medium CW4-v, medium CW5-,, medium CW5 -Ü, medium CW5-iii, medium CW5-iv, m edio CW5-V, medium CW6-i, medium CW6-Ü, medium CW6-¡¡¡, medium CW6-iv and / or CW6-V medium. 57. A method for the micropropagation of bamboo, which involves cultivating the explants and / or bamboo shoots in a means of mode 51, 52, 53, 54, or 55. 58. A method for the micropropagation of bamboo according to the method 57, where the bamboo is Phyllostachys bissetti; Fargesia denudata; Pleioblastus fortunei; Sasa Veitchii; Pleioblastus viridistriatus; Thamnocalamus crassinodus; Chusquea Culeo "Cana Prieta"; Bambusa Old Hamii; Phyllostachys Moso; Phyllostachys Atrovaginata; Dendrocalamus Asper; or Guadua Angustifolia. 59. A kit comprising a medium according to any of the embodiments 43, 44, 45, 46, 47, 50, 51, 52, 53, 54, 55, or 56.

The modalities also include all the modalities provided above where the bamboo that is micropropagated is Arundinaria gigantea; Bambusa balcoa; Bambusa vulgaris; Bambusa vulgaris 'Vitatta'; Bambusa Oldhamii; Bambusa tulda; endrocalamus brandesii; Dendrocalamus asper; Dendrocalamus hamiltoni; Dendrocalamus giganteus; Dendrocalamus membranaceus; Dendrocalamus strictus; Rough gigantochloa; Gigantochloa scortechini; Guadua culeata; uadua aculeata 'Nicaragua'; Guadua amplexifolia; Guadua angustifolia; Guadua angustofolia bi-color; Guadua paniculata; Melocanna bambusoides; eohouzeaua dullooa (Teinostachyum); Ochlandra travancorica; Phyllostachys edulis 'Moso'; Phyllostachys nigra; Phyllostachys nigra 'Henon'; or Schizostachyum lumampao.

Detailed description of the invention Bamboos are versatile plants with many different applications. They are a basic element in Asian cuisine and are found in a number of products that include toothpicks, brooms, poles for viticulture and arb'oricultivo, landscaping materials, parquet flooring, laminated materials, furniture, handicrafts and other household items . In addition, bamboo has become an important source of textile material as a component of paper production and as a source of structural wood.

Bamboo is considered an "ecological" product that does not harm the environment. One of the characteristics that gives bamboo its ecological reputation is its extremely rapid growth rate. Despite the rapid growth rate of bamboo, however, it has other characteristics that make it a difficult crop to control including its long development cycle and its tendency to exhibit a massive (or gregarious) development.

The embodiments described herein provide the micropropagation or tissue culture (these terms are used interchangeably herein) of the bamboo on a commercial scale.

The micropropagated plants grow in vitro in a sterile medium. The sterile medium can be liquid, semi-solid, or solid, and the physical state of the medium can be varied by the incorporation of one or more gelling agents. Any gelling agent known in the art to be suitable for use in a plant tissue culture medium can be used. The agar is the most used for this purpose. Examples of such agars include agar type A, E or M and ager Bacto ™. Other exemplary gelling agents include carrageenan, gellan gum (commercially available as PhytaGel ™, Gelrite® and Gelzan ™), alginic acid and its salts, and agarose. Mixtures of these agents, such as two or more of agar, carrageenan, gellan gum, agarose and alginic acid or a salt thereof, may also be used. Commonly, the medium comprises agar, with the addition of various compounds such as nutrients, inorganic salts, growth regulators, sugars, vitamins and other compounds. Other medium additives may include, but are not limited to, amino acids, macroelements, iron, microelements, inositol and undefined medium components such as casein hydrolysates or yeast extracts. For example, the medium can include any combination of NH4N03; KN03; Ca (N03) 2; K2S04; MgSO4; MnS04; ZnS04; CuS04; CaCl2; Kl; CoCI2; H3B03; Na2MoQ4; KH2P04; FeS04; Na2EDTA; Na2H2P04; myo-inositol; thiamine; pyridoxine; nicotinic acid; glycine; riboflavin; ascorbic acid; standard silicon solution; ß-naphthoxyacetic acid (NAA); indole-butyric acid (IBA); 3-indoleacetic acid (IAA); bencolaminopurine (BAP); 6-Y-Y- (dimethylallylamino) -purine (2-ip); sugar; agar; Carrageenan and charcoal. Examples of plant growth regulators include auxins and compounds with auxin-like activity, cytokinins, and compounds with cytokinin-like activity. Exemplary auxins include 2,4-dichlorophenoxyacetic acid, IBA, picloram and combinations thereof. Exemplary cytokinins, in addition to meta-topolin and thidiazuron, include adenine hemisulfate, benzyladenine, dimethylalyladenine, kinetin, zeatin and combinations thereof. same. Glyberic acid can also be included in the medium. A sugar can be included in the medium and can serve as a carbon source. Such sugars are known to those skilled in the art. Exemplary sugars include sucrose, glucose, maltose, galactose and sorbitol or combinations thereof.

The specialized means, systems and methods that allow the cultivation of correct bamboo tissue on a commercial scale are described herein. A certain medium described herein includes cytokinins, meta-topolin and / or thidiazuron. While certain embodiments use meta-topolin and / or thidiazuron defined later as particular compounds, other related compounds may also be correct.

The compounds useful according to the present disclosure include meta-topolin analogues having a general formula wherein W is an aryl or heteroaryl; R1 is substituted or unsubstituted alkyl wherein any C in the akyl may be substituted with O, N or S; each R2 is independently H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, cyano, alkyloxy of 1 to 6 carbon atoms, aryl or heteroaryl each optionally substituted with alkyl of 1 to 6 carbon atoms, SH, NHR3, C02R3 or halogen; R3 is H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, a carboxylic group, ester group, aldehyde or cyano; r is 0 to 8.

In one modality, W is where a dashed line represents the presence or absence of a link; X-X7 each is independently selected from C, N O, S provided that X joining the ring to N is C. another modality, the compounds have a structure where a dashed line represents the presence or absence of a link.

In another embodiment, the compounds have a structure where a dashed line represents the presence or absence of a link; X8-X12 each is independently selected from C, N, O, S; each R 4 is independently H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, cyano, alkyloxy of 1 to 6 carbon atoms, aryl or heteroaryl each optionally substituted with alkyl of 1 to 6 carbon atoms, SH, NHR3, C02R3 or halogen; R 3 is H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, at 1 to 6 carbon atoms, halogen, carboxyl group, ester group, aldehyde or cyano; p is 0 to 5; Y q is 0 to 6.

In other embodiments, the compounds have a structure In yet another embodiment, the compounds have a structure In still another embodiment, the compounds may have the structures selected from In one embodiment, R4 is OH.

In another embodiment, the compounds have a structure selected from In another embodiment, the compounds have a structure where a dashed line represents the presence or absence of a link.

In another embodiment, the compounds have a structure where a dashed line represents the presence or absence of a link; X8-X12 each is independently selected from C, N, O, S; each R 4 is independently H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, cyano, alkyloxy of 1 to 6 carbon atoms, aryl or heterparyl each optionally substituted with alkyl of 1 to 6 carbon atoms, SH, NHR3, C02R3 or halogen; R3 is H, OH, alkyl of 1 to 6 carbon atoms, the alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, carboxylic group, ester group, aldehyde or cyano; p is 0 to 5; Y q is 0 to 6.

In other embodiments, the compounds have a structure In yet another embodiment, the compounds have a structure In one embodiment, the compound is meta-topolin, also known as 6- (3-hydroxy-benzylamino) -purine, and by the abbreviation mT, which has a molecular formula of C 12 H 10 N 5 OH, a molecular weight of 241.25, and the following formula structural: where the meta-topolin is a derivative of a willow tree or poplar tree.

The compounds useful according to the present disclosure include thiadiazuron analogs having a general formula wherein V is an aryl or heteroaryl; each 5 and R6 is each independently H, OH, alkyl of 1 to 6 carbon atoms, alkylene of 1 to 6 carbon atoms, alkyl of 1 to 6 carbon atoms, halogen, cyano, alkyloxy of 1 to 6 atoms carbon, aryl or heteroaryl each optionally substituted with alkyl of 1 to 6 carbon atoms or halogen; n is 0 to 4; or it's 0 to 5 X13-X16 each is independently selected from C, N, O, S; Z and Z2 are each independently NH, O, SH or CH or Z1 and Z2 can be combined to form a substituted or unsubstituted aryl or heteroaryl; Y Y1 is O or S.

In another embodiment, the compounds have a structure wherein X17-X21 each is independently selected from C, N, O, S.

In other embodiments, the compounds include In one embodiment, the compound is thidiazuron, also known as 1-phenyl-3- (1, 2,3-thiadiazol-5-yl) urea. and 5-phenylcarbamoylamino-1,2,3-thiadiazole has the molecular formula of CgHsls OS, a molecular weight of 220.25 and the following structural formula If present in a medium, each cytokinin can be present in an amount of 0.001 mg / l-100 mg / l and all amounts between that interval. In certain embodiments, the meta-topolin and / or its analogs may be present at 0.001 mg / L, 0.01, 0.1, 1, 2, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5: 6, 5.7, 5.8, 5.9, 6, 6.1. 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 mg / l. In particular embodiments, thidiazuron and / or its analogues may be present at 0.001 mg / L, 0.01, 0.025, 0.05, 0.075, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6 , 0.65, 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1.0, 1.25, 1.50, 1.75, 2.25, 2.5, 2.75, 3.5, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, '50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61 , 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86 , 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 mg / l.

When both are used, meta-topolin and / or its analogs and thidiazuron and / or its analogues can also be included in ratios. For example, the amount of meta-topolin and / or its analogues to thidiazuron and / or its analogs may be 100: 1, 95: 1, 90: 1, 85: 1, 80: 1, 75: 1, 70: 1., 65: 1, 60: 1, 55: 1, 50: 1, 45: 1, 40: 1, 35: 1, 30: 1, 29: 1, 28: 1, 27: 1, 26: 1 25: 1, 24: 1, 23: 1, 22: 1, 21: 1, 20: 1, 19: 1, 18: 1, 17: 1, 16: 1, 15: 1, 14: 1, 13 : 1, 12: 1, 11: 1, 10: 1; 9: 1, 8: 1, 7: 1, 6.9: 1, 6.8: 1, 6.7: 1, 6.6: 1, 6.5: 1, 6.4: 1, 6.3: 1, 6.2: 1, 6.1: 1, 6: 1, 5.9: 1, 5.8: 1, 5.7: 1, 5.6: 1, 5.5: 1, 5.4: 1, 5.3: 1, 5.2: 1, 5.1: 1, 5: 1; 4: 1, 3: 1, 2: 1, 1: 1, 0.75: 1, 0.5: 1, 0.25: 1, 0.1: 1, 0.075: 1, 0.05: 1, 0.025: 1 or 0.001: 1. When meta-topolin and thidiazuron are used, they may be present in the same or different medium.

NAA, BAP, 2ip and / or IBA can similarly be present in 0.001 mg / l, 0.01, 0.1, 1, 2, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6 , 6.7, 6.8, 6.9, 7, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32 , 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57 , 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82 , 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 mg / l or 0.001 mg / l, 0.01, 0.025, 0.05 , 0.075, 0.1, 0.15, 0.2, 0.25, 0.3, 0.35, 0.4, 0.45, 0.5, 0.55, 0.6, 0.65. 0.7, 0.75, 0.8, 0.85, 0.9, 0.95, 1.0, 1.25, 1.50, 1.75, 2.25, 2.5, 2.75, 3.5, 4.5, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 or 100 mg / l.

The structures or formula for a number of chemical compounds, which include meta-topolin and thidiazuron, have been previously provided. One skilled in the art will recognize that a compound should be broadly construed as including pharmaceutically acceptable salts, prodrugs, tautomers, alternating solid forms, non-covalent complexes, analogs, derivatives, and combinations thereof, a chemical entity of the chemical name or structure represented.

A pharmaceutically acceptable salt is any salt of the parent compound that is suitable for use in the methods described herein. A pharmaceutically acceptable salt also refers to any salt that can be formed as a result of the administration of an acid, another salt, or a prodrug that becomes an acid or salt. A salt comprises one or more ionic forms of the compound, such as a conjugated acid or base, associated with one or more corresponding counterions. The salts can be formed of or incorporate one or more deprotonated acid groups (e.g., carboxylic acids), one or more protonated basic groups (e.g., amines), or both (e.g., zwitterions).

It is not intended to be limited by the compounds described above, various tautomers of the above compounds may be possible. As used herein, the "tautomer" refers to the migration of protons between adjacent single and double bonds. The tautomerization process is reversible. Other possible tautomers when the compound includes, for example, but not limited to, enol, keto, lactam, amide, imide, amine, and imine groups. The tautomers will generally reach a state of equilibrium wherein the double bond is shared in a resonant manner between the two link lengths.

Unless the stereochemistry is represented explicitly, a structure is intended to include every possible stereoisomer, pure or in any possible mixture. ' Alternating solid forms are different solid forms than those that can result from practicing the procedures described herein. For example, the alternate solid forms can be polymorphs, different kinds of solid amorphous forms, glasses, and the like.

The non-covalent complexes are the complexes that can be formed between the compound and one or more additional chemical species that do not involve a covalent bond interaction between the compound and the additional chemical species. They may or may not have a specific relationship between the compound and the additional chemical species. Examples may include solvates, hydrates, charge transfer complexes, and the like.

As a description, in common micropropagation, plants are placed in various media that stimulate physiological processes such as growth and multiplication and / or within the plant. The process generally includes 3 steps (after the preparation and disinfection of the explant, discussed below): (1) initiation of growth in vitro and / or multiplication of the explant in a medium; (2) multiplication of an additional vitro in a second medium; and (3) transition to ex vitro conditions. Not every tissue culture process requires each stage, however, and in certain processes, the stages can be combined or omitted. For example, while there is commonly a change in media types between stages 1 and 2, in certain modalities, a medium change is not included. In other processes, plants may not require a particular stage that promotes the transition to ex vitro conditions but instead concludes the process in the same media that supports multiplication. Accordingly, as described herein, the means are defined as a means of stage 1 (first means of a process); Stage 2 means (2nd middle of a process); middle of stage 3 (3rd middle of a process); etc. The particular medium can change the stage based on the number of stages within a particular process and where the particular medium resides within its order.

To begin the process, the medium of stage 1 can be obtained or prepared. The medium of stage 1 includes a pH that is generally flexible for plants (commonly 4.0-7.0 or 4.5-6.5). The stage 1 medium is then placed in test tubes or other appropriate containers (including jars, boxes, jugs, cups, etc., where when not specified they are collectively referred to as "tubes"). These tubes can be capped or covered and sterilized in an autoclave to sterilize the tubes and medium. In another embodiment, sterilization is achieved by autoclaving at 0.34-1.72 bar (5-25 psi) at a temperature of 93.33 ° C (200 ° F) for 10-25 minutes. In another embodiment, sterilization is achieved by autoclaving at 1.03 bar (15 pounds psi) at a temperature of 121.11 ° C (250 ° F) for 15-18 minutes. Sterility can also be determined by an accepted number of tubes contaminated by one hundred tubes, for example and without limitation, 0 tubes contaminated by one hundred tubes, no more than 1 tube contaminated by one hundred tubes, no more than 2 tubes contaminated per one hundred tubes, no more than 3 tubes contaminated by one hundred tubes, no more than 4 tubes contaminated by one hundred tubes, no more than 5 tubes contaminated by one hundred tubes, no more than 6 tubes contaminated by one hundred tubes, no more than 7 tubes contaminated by one hundred tubes, no more than 8 tubes contaminated by one hundred tubes, no more than 9 tubes contaminated by one hundred tubes, no more than 10 tubes contaminated by one hundred tubes, etc.

In the medium containing a gelling agent, such as agar, agarose, gellan gum, carrageenan or combinations thereof, the medium solidifies during cooling and serves to provide micropropagated plant tissues with support, nutrients, growth regulators , water and other compounds as described later. Generally, the tubes and jars contain 15-25 ml medium while the boxes contain 40-50 ml medium. The cups can include 30-40 ml while the jars usually contain more than 50 ml.

The micropropagated plants start from a selected piece of plant tissue, called "explant" or "mother plant". This explant is the source of the cells that will develop during the tissue culture process. The explant can be any segment or collection of apical meristem cells, axillary buds, cambium, lateral meristem, shoot apexes, stem segments, nodal nodal sections of shoots, lateral shoots, seedling or leaf segments. In one embodiment, the explant is taken from a 1-year-old bamboo plant. In another modality, the explant is taken from a 2-year-old bamboo plant. In another modality, the explant is taken from a bamboo plant that is 5 years old or less. In another modality, the explant is taken from a bamboo plant that is 4 years old or less. In another modality, the explant is taken from a bamboo plant that is 3 years old or less. In another modality, the explant is taken from a bamboo plant that is 2 years old or less. In another modality, the explant is taken from a bamboo plant that is 1 year old or less. In another modality, the explant is taken from a bamboo plant that is 6 months or less. In another modality, the explant is taken from a bamboo plant that is 3 months or less. The bamboo from which the explant is obtained can be developed in any convenient agricultural situation, including but not limited to growth in a growth chamber, growth in a greenhouse or growth in a field.

As will be understood by one skilled in the art, a variety of appropriate explants can be used in accordance with the present disclosure. In certain embodiments according to the present disclosure, the immature nodal sections of the stems can be used as explant material. In one embodiment, the explants may be new growth rods with lateral shoots that barely break the sheath in the nodal sections. The new growth rods include those obtained from the plant within a current season or year, where such new growth rods can be obtained from any node in the plant. In a particular embodiment, the explant material includes or is limited to the third base node of a rod.

The nodal sections can be cut into sections of 3-5, 1-10, 2-9, 3-8, 4-6, 3-6 or 2-7 millimeters with the shoot intact and disinfected to eliminate pathogens on the outside of the explant. Any disinfection method known in the art can be used. Exemplary disinfection methods include the use of a disinfectant, such as a disinfectant selected from bleach (sodium hypochlorite and / or potassium and / or calcium), alcohol (eg, ethanol, isopropyl), ozone, chlorine gas, solution of iodine or antibiotic or antifungal solution or combinations thereof, or subjecting the exposed surface of the explant to ultraviolet light or to a temperature of -20 ° C or less or to a temperature higher than 40 ° C or 50 ° C for a short period of time. In certain modalities, small amounts (a few drops) of Tween 20 can be added to the disinfection solutions.

After initial disinfection, the outer sheaths can be peeled off and discarded and the part placed in approximately 1%, 5%, 10%, 15%, 20%, 25% or 30% of a solution of a commercial bleach or a solution similar disinfectant. The peeled explant in the disinfectant solution can be placed on a vibrating table, such as, for example, an orbital shaker Barnstrea / Lab line, adjustable speed with laboratory rotators (model number KS 260) for 10 minutes, 20 minutes, 30 minutes. minutes, 60 minutes, 90 minutes, 120 minutes, 150 minutes, 180 minutes or 210 minutes at 6-9 or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12, 13, 14, or, 15 revolutions per minute. In another embodiment, the detached explants can then be placed in a solution of approximately 1% bleach or similar disinfectant solution, and placed back on the vibrating table for 30 minutes. In another embodiment, this 1% bleach or similar disinfectant solution step can be repeated. In another modality, these described stages are progressive and include all disinfection processes. As will be understood by one skilled in the art, a variety of appropriate disinfection procedures can be used in accordance with the present disclosure.

Once they are disinfected, the explants can be placed in a medium of stage 1 inside the tube and the tubes can be placed in a regulated growth chamber. As used herein, "growth chambers" may include a number of configurations and sizes including box tables, independent chambers, cabinets, small rooms, large rooms, etc. As understood by one skilled in the art, variables such as light or temperature can be appropriately controlled in such growth chamber. The appropriate intervals for bamboo tissue culture include 18.33 ° C-21.11 ° C, 15.55 ° C-23.88 ° C or 12.770C-26.66 ° C at 200-500, 150-550 or 100-600 lumen per square foot . The illumination may be of a full spectrum, although alternative illumination systems may also be used according to the present invention.

The explants allow to establish themselves inside the tubes while they are in the growth chamber in the middle of stage 1. In the most common tissue culture of stage 3, (ie growing without visible contamination), the cell cultures Growing from the explants are transferred in a second, middle of stage 2. Alternatively, once established, the cell cultures can remain in a medium of stage 1. At this stage in the tissue culture process, a large number of plants can be created within a relatively short period of time because each cell culture can develop multiple shoots and each shoot can be separated and placed in an individual tube where it will develop additional shoots to separate and multiply.

Without limiting the medium at a particular stage, non-limiting examples of the medium that commonly serves as a means of stage 1 and / or stage 2 includes: Medium b-12c (1-v): Half CW2 (i-v): Medium b-9 (v): Half CW4 (i-v): Medium CW1 (i-v): Half CW5 (i-v): Half CW6 (i-v): Medium b-10 (¡-v): Medium b-11 (i-v): Medium b-1 (i-v): Medium b-4 (i-v): Medium b-6 (i-v) Observe that for each of these means, its solid form is provided. Each of the media can be transformed into a liquid medium by removing agar and carrageenan and liquid forms from these media and their uses are expressly included within the scope of the present invention.

The expected number of shoots may be different at different stages of the tissue culture process and may also depend on the bamboo species. Generally, however, at the beginning of the process, multiplication is from I.0-2.0, 1.0-3.0 or 2.0-3.0 times. Once it is established, the multiplication may depend on the chosen container. For example, multiplication can have a range of, without limitation, 1-10 or 2-6 plants per tube, 1-15 or 4-9 plants per container, 1-20 or 9-17 plants per box or 1-50 or 20-35 plants per container. Number 1 is included because certain species or particular cell cultures require more time in the middle of stage 1 or stage 2 before multiplication begins. Taking them through the process, however, most or almost all begin their multiplication within a number of cycles. For example, some cell cultures may begin to multiply only after 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, I, 12, 13, 14, 15, 16, 17 or 18 months in culture.

The methods described herein may yield the following non-limiting number of shoots of a single explant: 100, 500, 1,000; 5,000; 10,000, 20,000, 50,000, 100,000, 250,000, 500,000, 750,000, 1,000,000 or more.

Following multiplication through cultivation and subculture, the shoots of particular plants can be selected by transition to ex vitro conditions. Generally, the medium that supports the transition to ex vitro conditions represents a means of a stage 2, stage 3, stage 4 or stage 5. Non-limiting examples of such means include: Means Ech (i-v): Medium BR-2 (i-v): Half Amel (i-v): During the transition to ex vitro conditions, shoots and media can be placed in air-tight or air-permeable containers.

Each of the means described herein may be used in combination with one of each medium in a method, system or kit described herein. On the other hand, the medium may be combined in combinations greater than two (for example, a kit may include 2 of the different media provided herein, or include 3 of the different media provided herein, or include more than 3 of the different means described herein). While not every possible combination is explicitly described herein, one skilled in the art will understand that this invention supports all possible combinations.

Following the transition to ex vitro conditions, but before the plants are placed on the ground, or exposed to less regulated growth conditions, the plants can undergo a series of treatments designed to acclimate them to an environment of unregulated growth. This is because some plants, when micro-cultivated, do not develop adequate defensive structures, such as waxy cuticles to protect them against ordinary environmental conditions. Treatments that plants may undergo before exposure in an unregulated environment may include, without limitation, acclimatization to moisture, acclimation to variations in temperature, and acclimation to wind pressure. These acclimatization factors can be introduced gradually and / or in a staggered manner.

The representative representative genus of bamboo for use with the descriptions herein includes: Acidosase; Ampelocalamus; Arundinaria Bambusa; Bashania; Borinda; Brachystachyum; Cephalostachyum; Chimonobambusa; Chimonocalamus; Chusquea; Dendrocalamus; Dinochloa; Drepanosíachyum; Eremitis; Fargesia; Gaoligongshania; Gigantochloa; Guadua; Hibanobambusa; Hima.layacalamus; Indccalamus; Indosase; Lithachne; Melocalamus; Melocanna; Menstruocalamus; Nastus; Neohouzeaua; Neololeba; Ochlandra; Oligostachyum; Olmeca; Otatea; Oxytenanthera; Phyllostachys; Pleioblastus; Pseudosasa; Raddia; Rhipidocladum; Sarocalamus; Sasa; Sasaella; Sasamorpha; Schizostachyum; Semi-redundant; Shibataea; Sinobambusa; Thamnocalamus; Thyrsostachys; and Yushania.

Unlimited examples of species within these genera include: , Acd d to: Edulis Ampelocalamus: Scandens Arundinaria: Arundinaria Appalachiana; Arundinaria funghomii; Arundinaria gigantea; Arundinaria gigantea "Macón"; and Arundinaria Tecta Bambusa: arnhemica; ba! cooa; bambos; basihirsuta; beecheyana; beecheyana var pubescens; blumeana; boniopsis; burmanica; chungii; chungii var. Barbelatta; cornice dissimulator; dissimulator 'Albinodia'; Disagree dolichoclada; dolichoclada 'Stripe'; dolichomerithalla Oreen stripe '; dolichomerithalla 'Silverstripe'; emeiensis 'Chrysotrichus'; emeiensis 'Flavidovirens'; emeiensis 'Viridifiavus'; eutuldoides; eutuldoides 'Viridivittata'; gibba glaucophylla; intermediate; lako; lapidea; longispiculata; maculata malingensís; multiplex; multiplex 'Alphonse Karr'; multiplex 'Fernleaf Stripestem'; multiplex 'Fernleaf; multiplex 'Golden Goddess'; multiplex 'Goldstripe'; multiplex 'Midori Green'; multiplex 'Riviereorum'; multiplex 'Silverstripe'; multiplex 'Tíny Fern Striped'; multiplex 'Tiny Fern'; multiplex 'Willowy'; nutans; odashimae; Odashimae X B. Tuldoides; oldhamii; oliveriana; pachinensis; pervariabilis; pervariabilis 'Viridistriatus'; rigid; rutila; sinospinosa; sp 'Hirose'; sp. 'Clone X'; sp. 'Nana'; sp. 'Polymorpha'; sp. 'Richard Waldron'; stenostachya; suberect; textilis; textilis' Dwarf; textile is 'Kanapaha'; textilis 'Maculata'; textilis 'Mutabilis'; textilis 'Scranton'; textilis var. Albostriata; textilis var. Glabra; textilis var. Gracilis; tulda; tulda 'Striatá'; Tuldoides; severaltriata; ventricular; ventricosa 'Kimmel'; vulgaris; vulgaris 'Vittata'; vulgaris 'Wamin Striata'; and vulgaris 'Wamin' Bashania: Fargesii; and Qingchengshanensis Borinda: KR 5288; Albocerea; Angustissima; Contract Frigidorum; Fungus; fungus "white cloud"; Lushuiensis; Macclureana; Nujiangensis; Papyrifera; Perlonga; SP. "Muliensis"; and Yulongshanensis Brachystachyum: densiflorum; and densiflorum var. villosum Cephalostachyum: Pergracile; and Virgatum Chimonobambusa: macrophylla "Intermediate"; Marmórea; marmoreal "Variegata"; Quadrangularis; quadrangularis 'Joseph de Jussieu'; quadrangularis 'Suow'; quadrangularis 'Yellow Groove'; Szechuanensis; and Tumidissinoda Chimonocalamus: Pallens Chusquea; Andean; Circinata; circinata 'Chiapas' Coronalis; Culeou; culeou 'Argentina'; culeou 'Caña Prieta'; culeou 'Hillier's Form'; Cumingii; Delicatula; Foliosa Galeottiana; Gigantea; Glauca; Liebmannii; Macrostachya; mimosa ssp. Australis; Montana; Muelleri; Pittieri; Simpliciflora; sp. 'Chiconquiaco'; sp. 'Las Vigas'; Subtilis; Sulcata; Tomentosa; Uliginous; Valdiviensis; and Virgata Dendrocalamus: Asper; asper 'Betung Hitam'; Brandisii; brandisii 'Black'; brandisii (variegated); Calostachyus; Giganteus; giganteus (Quail Clone); giganteus (variegated); Hamiltonii; Jianshuiensis; jianshuiensis (variegated); Latiflorus; latiflorus 'Mei-nung'; Membranaceus; Minor; minor 'Amoenus'; Sikkimensis; Sinicus; sp. 'Maroochy'; sp. 'Parker's Giant'; Strictus; Validus; and Yunnanicus Dinochloa: Malayana; and Scandens Drepanostachyum: falcatum var. sengteeanum; and Khasianum Eremitis: Eremitis Fargesia: Adpressa; Apircirubens; apircirubens 'White Dragon'; Denudata; dracocephala 'Rufa'; Murieliae; murieliae 'SABE 939'; murieliae 'Vampire'; murieliae (next generation of seedlings); Sharp sharp 'Jiuzhaigou'; Robusta; Robust 'Campbell'; robust 'Wolong'; sp. 'Scabrida'; and Utilis Gaoligongshania: Gaoiigongshania and Megalothyrsa Gigantochloa: Hitam Hijau; Albociliata; Apus; Atroviolacea; Atter; Hasskarliana; Levis; Luteostriata; Maximum; Pseudoarundinacea; Rídleyi; Robusta; sp 'Rachel Carson'; sp. 'Batí White Stripe'; sp. 'Sumatra 3751'; sp. 'Widjaja 3827'; and Wrayii Guadua: Amplexífolia; Angustifolia; angustifolia 'Bicolor'; angustifolia 'Less Thorny'; Chacoensis; Longifolia; Paniculata; sp.

'Aureocaulis'; and Velutina Hibanobambusa: Tranquillans; and tranquillans "Shiroshima" Himalayacalamus: Falconeri; falconeri "Damarapa"; Hookerianus; Planatus; and Porcatus Indocalamus: Cordatas; Latifolius; latifolius "Hopei; Longiauritus; sp." Hamadae "; sp." Solidus "; Tessellatus; and Victorialis Indosase: Crassiflora; and Gigantea Lithachne: Humilis elocalamus: Arrectus Me! Ocanna: Baccifera Menstruocalamus: Sichuanensis Nastus: Elatus Neohouzeaua: Mekongensis Neololeba: Atra Ochlandra: Stridula Oligostachyum: Glabrescens Olmeca: Straight Otatea: acuminata "Michoacan"; acuminata ssp Acuminata; acuminata ssp. Aztecorum; acuminata ssp. aztecorum 'Dwarf; Fimbriata; and glauca 'Mayan Silver' Oxytenanthera: Abyssinica; and Braunii Phyllostachys: Acula; Angusta; Arcane; arcana 'Luteosulcata'; Atrovaginata; Aurea; golden 'Albovariegata'; Golden 'Dr Don'; golden 'Flavescens-inverse'; Golden 'Holochrysa'; Aurea 'Koi'; Golden 'Takemurai'; Aureosulcata; aureosulcata 'Alata'; aureosulcata 'Aureocaulis'; aureosulcata 'Harbin Reverse'; aureosulcata 'Harbin'; aureosulcata 'Pekinensis'; aureosulcata 'Spéctabilis'; Aurita; Bambusoides; bambusoides 'Albovariegata'; bambusoides 'Reverse Castillon'; bambusoides 'Castillon'; bambusoides 'Golden Dwarf'; bambusoides 'Job's Spot'; bambusoides 'Kawadana'; bambusoides 'Marliac'; bambusoides' Rib Leaf; bambusoides 'Richard Haubrich'; bambusoides 'Slender Crookstem'; bambusoides 'Subvariegata'; bambusoides 'Tanakae'; bambusoides 'White Crookstem'; Bissetii; bissetii 'Dwarf; Dulcís; Edulis; edulis 'Anderson'; edulis 'Bicolor'; edulis 'Goldstripe'; edulis 'Heterocycla'; Elegans; Flexuosa flexoosa 'Kimmei'; Glauca; glaucous 'Notso'; glauca 'Yunzhu'; Heterolate; heteroclada 'Purpure it'; heterozylated 'Solidstem'; Hispide; Humilis; Incarnate; Iridescens; Kwangsiensis; Lithophila; Lofushanensis; Makinoi; mannii 'Decorate'; Mannii 'Mannii'; Meyeri; Nidularia Nidularia 'Farota'; nidularia 'Smoothsheath'; Nigra; nigra 'Bory'; nigra 'Daikokuchiku'; nigra 'Hale'; nigra 'Henon'; nigra 'Megurochiku'; nigra 'Mejiro'; nigra 'Muchisasa'; nigra 'Othello'; nigra 'Punctata'; nigra 'Shimadake'; nigra 'Tosaensis'; Nuda; nuda 'Localis'; Parvifolia; Platyglossa; Praecox; praecox 'Prevernalis'; praecox 'Viridisulcata'; Prominens; Propinqua; propinqua 'Beijing'; Robustiramea; Rubromarginata; Stimulosa; Varioauriculata; Violascens; Viridiglaucescens; Viridis; viridis 'Houzeau'; viridis 'Robert Young'; Vivax; vivax 'Aureocaulis'; vivax 'Black Spot'; vivax 'Huangwenzhu Reverse'; yd vivax 'Huangwenzhu' Pleioblastus: Akebono; Amarus; Argenteostriatus; Chinese; Chinese 'Angustifolia'; Chinese 'Elegantissimus'; Chinese 'Kimmei'; Chinese 'Murakamiansus'; Chinese 'Vaginatus Variegatus'; Distichus; distichus 'Mini'; Fortunei; Gauntlettü; Gramineus; gramineus 'Monstrispiralis'; Hindsii; Humiiis; humilis 'Albovariegatus'; humilis 'Variegatus'; Juxianensis; Kodzumae; Kongosanensis; kongosanensis 'Akibensis'; kongosanensis 'Aureostriatus'; Linearis; linearis 'Nana'; Nagashima; Oleosus; Pygmaeus; pygmaeus 'Greenstripe'; pygmaeus 'Ramosissimus'; shibuyanus 'Tsuboi'; Simonii; simonii 'Variegatus'; Viridistriatus; viridistriatus 'Chrysophyllus'; and Xestrophyllus Pseudosasa: Amabilis; Cantori; Guanxianensis; Japan Japan 'Akebono'; japonica 'Akebono-suji'; japonica 'Pleioblastoides'; Japan 'Tsutsumiana'; japónica 'Variegata'; Longiligula; Owatarii; Usawai; and Viridula Raddia: Brasiliensis; and Distichophylla Rhipidocladum: Pittieri; and Racemiflorum Sarocalamus: Faberi; and Fangianus Sasa: Cernua; Gracillima; Hayatae; Kagamiana; kagamiana ssp. Yoshinoi; Kurilensis; kurilensis 'Shimofuri'; Megalophylla; Nagimontana; nipponica (hort.); Oshidensis; Palmata; Senanensis; Shimidzuana; sp. Tsuboiana; and Veitchii Sasaella: Bitchuensis; hidaensis "muraii"; Masamuneana; masamuneana "Albostriata"; masamuneana "Aureostriata"; Ramosa; Sasakiana; and Shiobarensis Sasamorpha: Borealis Schizostachy um: Brachycladum; brachycladum 'Bali Kuning'; Caudatum; Glaucifolium; Jaculans; Lime; and sp. 'Murray Island' Arundinary Semira: Fastuosa; lavish 'Viridis'; Fortis; Kagamiana; Lubricate; Makinoi; Okuboi; sp. Maruyamana; sp. 'Korea'; Yashadake; yashadake 'Kimmei'; and yashadake 'inverse kimmei' Shibataea: Chinensis; Kumasaca; kumasaca 'Albostriata'; kumasaca 'Aureostriata'; Lancifolia; and Nanpingensis Sinobambusa: Gigantea; Intermediate Tootsik; and tootsik "Albostriata" Thamnocalamus: aristatus' Aristatus hort. US '; Crassinodus; crassinodus 'Kew Beauty'; crassinodus 'Mendocino'; crassinodus 'Merlyn'; nepalensis 'Nyalam'; Spathiflorus; and Tessellatus Thyrsostachys: Oliven '; and Slamensis Yushania: Alpina; Anceps; anceps 'Pítt White'; Boliana; Brevipaniculata; Exilis; Macúlate and maling Particularly useful species include: edulis; scandens; ArundinariaGigantea; ArundinariaTecta; BambusaBalcooa; BambusaBambos; Bambusa Oldhamii; BambusaTextilis; BambusaTulda; BashaniaFargesii; BrachystachyumDensiflorum; ChusqueaGigantea; DendrocalamusAsper; DendrocalamusBrandisii; DendrocalamusGiganteus; DendrocalamusHamiltonii; DendrocalamusSt rictus; FargesiaDenudata; Fargesiadracocephala'Rufa '; FargesiaMurieliae; Clear Fargesia; FargesiaRobusta; Fargesiarobusta 'Wolong'; Fargesiasp.

'Scabrida'; GuaduaAmplexifolia; Guadua Paniculata; Himalayacalamus Falconeri; Indocalamus Tessellatus; Ochlandra Stridula; Otatea acuminata ssp. Aztecorum; Phyllostachys Atrovaginata; Phyllostachys Aurea; Phyllostachys Bambusoides; Phyllostachys Bissetü; Phyllostachys Edulis; Phyllostachys edulis 'Heterocycla'; Phyllostachys Glauca; Phyllostachys Iridescens; Phyllostachys Kwangsiensis; Phyllostachys Nidularia; Phyllostachys Nigra; Phyllostachys nigra 'Henon'; Phyllostachys Nuda; Phyllostachys Parvifolia; Phyllostachys Praecox; Phyllostachys Propinqua; Phyllostachys Viridis; Phyllostachys Vivax; Pleioblastus Distichus; Pleioblastus Fortunei; Pleioblastus Linearis; Pseudosasa Japan Sasa Kurilensis; Sasa Veitchii; Sasaella Masamuneana; Sasamorpha Borealis; Schizostachyum Brachycladum; Schizostachyum brachycladum 'B a I i Kuning'; Schizostachyum Caudatum; Schizostachyum Glaucifolium; Schizostachyum Jaculans; Schizostachyum Lima; Schizostachyum sp. 'Murray Island'; Semi-weekly Fastuosa; Semiarundinaria Yashadake; Shibataea Kumasaca; Sinobambusa Gigantea; Thamnocalamus Crassinodus; Thamnocalamus Tessellatus; Yushania Alpina; and Yushania maling.

As an expert in the art appreciates, many species of bamboo have different common names. Accordingly, the following terminology and language comparisons are provided.

Chinese names & Japanese Chimonobambusa Phyllostachys Han zhu M adake bambusoides marmoreal Hong Bian Phyllostachys Pleioblastus Medake zhu rubromarginata simonii Phyllostachys Phyllostachys Hou zhu Moso nidularia edulis H U Í Xiang Chimonocalamus Semiarundinaria Narihira zhu pallens lavish Phyllostachys Okame- Shibataea Jin zhu sulphaurea zasa kumasaca Dendrocalamus Pseudosasa to zh u Yadake latiflorus japonica Phyllostachys Mao Zhu edulis Chimonobambusa Qiong zhu tumidissinoda Ren Mian Phyllostachys zhu aurea Phyllostachys Shui zhu heterocycled Phyllostachys Wu Ya zhu atrovaginata Xiang Nuo Cephalostachyum zhu pergracile Phyllostachys Zi zhu nigra Names in English Bambusa Chimonobambusa Common Square vulgaris quadrangularis Dwarf Fern Pleiobiastus Phyllostachys Stone Leaf distichus m angusta & P. nuda Dwarf Pleioblastus Phyllostachys Sweetshoot Whitestripe fortunei dulcís Bambusa Switch Fernleaf multiplex Arundinaria tecta Cane 'Fernleaf Pseudosasa Fountai n Fargesia neat Tea Stick loves bile Giant Bambusa Semiarun din a ría Temper Thorny lavish bambos Giant Bambusa Timor Bambusa lako Timber oldhamii B lack Green Tonkin Pseudosasa Yushania alpine Mountain Cane loves bile Phyllostachys Phyllostachys Tortoise Golden edulis Golden Shell 'Heterocycla' Phyllostachys Golden Tropical Glgantochloa aurea Golden Black atrovíolacea 'Holochrysa' 1 Technically not only a bamboo was included within the meaning of bamboo in the present.

By means of the means, systems and methods described and manifested herein, it is possible for a person skilled in the art to achieve successive bamboo tissue cultures. As used herein, "successive tissue culture" means that the multiplication process can continue substantially and indefinitely by continuing to separate and multiply outbreaks. In one embodiment, an outbreak is placed in a tube and the outbreak is multiplied by a number of additional outbreaks. After multiplication, each bud or a subset of the buds are separated and each is placed in a subsequent tube for further multiplication. This process can continue while at various times, some or all of the outbreaks can be eliminated from the process of multiplication and transition to ex vitro conditions. By continuing indefinitely, it is understood that 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 , 27, 28, 29.30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, etc., multiplication cycles per day can be repeated without initiating new explants for at least 1 month, at least 3 months, for at least 6 months, for at least 9 months, for at least 12 months, for at least 15 months, for at least 18 months, for at least 21 months, for at least 24 months or at least 36 months. Particular intervals of days in multiplication cycles include 10-120 days; 10-100 days; 10-80 days; 10-60 days; 10-42 days; 10-40 days; 10-20 days; 14-120 days; 14-90 days; 14-70 days; 14-50; 14-42 days; 14-30 days; 14-21 days; 12-42 days; 20-60 days; 10-15 days; 14-20 days; 14-18 days etc.

These media systems and methods can be packaged and / or described in various kits. The kits may include, without limitation, one or more of the following in a package or container: (1) one or more means; and (2) one or more explants of one or more bamboo species. In certain non-limiting modes, the media can be the medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-i , medium CW2-M, medium CW2-iii, medium CW2-iv, medium CW2-V, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i, medium b -12c-ii, medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b -1-iv, medium b-1-, medium b-4-i, medium b-4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b- 6-i, medium b-6-ii, medium b-6-iii, medium b-6-iv, medium b-6-, medium CW1-Í, medium CW1-Ü, medium CW1-iii, medium CW1- iv, medium CW1-V, medium CW3-i, medium CW3-ii, medium CW3-iii, medium CW3-iv, medium CW3-v, medium CW4-i, medium CW4-Ü, medium CW4-Ü, medium CW4- iv, medium CW4-v, medium CW5-Í, medium CW5-Ü, medium CW5-i¡¡, medium CW5-iv, medium CW5-v, medium CW6-Ì, medium CW6-Ü, medium CW6-ÌM, medium CW6-iv, medium CW6-v, medium Br-2-i , medium Br-2-ii, medium Br-2-iii, medium Br-2-iv, medium Ech-i, medium Ech-ii, Ech-iii medium, medium Ech-iv, medium Amel-i, medium Amel- ii, half Amel-üi, half Amel-iv or half Amel-v. In another embodiment, the kits may comprise one or more containers for the tissue culture process including without limitation, tubes, stirring vessels, boxes or jars. In another embodiment the kits may comprise instructions for growing bamboo tissue. In another embodiment, the kits comprise combinations of the foregoing. The components of several kits can be found in the same or different containers. Additionally, when a kit is supplied, the different components of the medium can be packaged in separate containers and mixed immediately before use. Such packaging of the components can separately allow long-term storage without losing the functions of the active components. Alternatively, the media can be provided premixed.

The ingredients included in the kits can be supplied in containers of any kind. The life of the different ingredients is preserved and not adsorbed or altered by the material is from the container. For example, sealed glass ampoules may contain ingredients that have been packaged under a neutral, unreacted gas, such as nitrogen. The amulets may consist of any suitable material, such as glass, organic polymers, such as polycarbonate, polystyrene, etc. , ceramic, metal or any other material normally used to maintain similar ingredients. Other examples of suitable containers include simple bottles that can be made from similar substances such as ampoules, and sachets, which may comprise interiors coated with aluminum paper, such as aluminum or an alloy. Other containers include test tubes, flasks, flasks, bottles, syringes, or similar. The containers may have a sterile access port, such as a bottle having a plug that can be pierced. Other containers may have two compartments that are separated by an easily removable membrane that upon removal allows the ingredients to mix. The removable membranes can be glass, plastic, rubber, etc.

As indicated, the kits can be supplied with instructional materials. The instructions can be printed on the paper or other substrate, and / or can be supplied as a readable electronic media, such as a floppy disk, C D-ROM, DVD-RO M, Zip disk, videotape, audiotape. io, etc. Detailed instructions can not be physically associated with the kit; Afterwards, a user can go to a website on the Internet specified by the manufacturer or distributor of the kit, or provided as an e-mail.

An advantage of the described methods is that the methods are more resistant than those previously used, producing plants that do not require special treatments is required by those produced using methods of the prior art. For example, methods d written herein do not require the use of seeds or inflorescence to generate the plants; do not require selection of diseased starter plants (such as those exhibiting symptoms of witch's broom disease or small leaf); they do not require the use of somatic embryogenesis and do not use pseudospigilas. For successful growth followed by tissue culture, the plants produced do not require irrigation directly in the pot, but remain robust with irrigation from above and do not require multiple adjustments to the intensity conditions of lz or moisture before transferring to a greenhouse or other growth conditions. These improvements over previous methods provide even additional advantages related to the salt of plants produced and growth and process efficiency.

The non-limiting embodiments encompassed by the present disclosure include (Step 1, Step 2, Step 3, etc., the means are defined elsewhere herein); I. The following species: Arundinaria gigantea; Bambusa balcoa; Bambusa vulgaris; Bambusa vulgaris 'Vitatta'; Bambusa Oldhamü; Bambusa tulda; endrocalamus brandesii; Dendrocalamus asper; Dendrocalamus hamiltoni; Dendrocalamus giganteus; Dendrocalamus membranaceus; Dendrocalamus strictus; Rough gigantochloa; Gigantochloa scortechini; Guadua culeata; uadua aculeata 'Nicaragua'; Guadua amplexifolia; Guadua angustifolia; Guadua angustofolia bi-color; Guadua paniculata; Melocanna bambusoides; eohouzeaua dullooa (Teinostachyum); Ochlandra travancorica; Phyilostachys edulis 'Moso'; Phyilostachys nigra; Phyilostachys nigra 'Henon'; Schizostachyum lumampao; II. Means of stage 1: medium b-12-c-v or medium b-10- ?; III. Means of Step 2: medium CW1-v; CW2-v medium; CW3-v medium; CW4-v medium; CW5-v medium; or CW6-V medium for 10-120 cycles per day; Y IV. Means of Stage 3: medium Br-2- ?; half Ech-v or half Amel-v.

More particularly, the following modalities can be used (Stage 1, Stage 2, Stage 3, etc., the means are defined elsewhere in the present): Starting with a bamboo plant between the ages of 3 months and 3 years, a cane node with the lateral bud that recently broke the pod can be used as the explant.

Each nodal section can be cut into sections of 3-5 millimeters with the bud intact. The outer sheaths can be peeled and discarded and the remaining nodal section piece is placed in 10% bleaching solution with a final concentration of 0.6% sodium hydrochlorides. The explant in bleaching solution can be placed on a vibrating table with orbital shaker line Barnstead / Lab, adjustable speed, with laboratory rotators, (model number KS 260) for 1 hour at 6-9 revolutions per minute. The explants can then be placed in 1% bleach solution with a final concentration of 0.06% sodium hydrochloride, and placed on the stirrer board again for 30 minutes. This 1% bleaching solution step can then be repeated.

Individual explants can then be placed in a medium of stage 1 (15-25 ml) inside a tube and the tubes can be placed in a clean growth chamber regulated at a temperature of 36.08 ° C-38.85 ° C (65 ° F) -70 ° F) and a full spectral light level of 60-150 spark-meters (200-500 spark plug-feet). The initial Stage 1 medium can be b-12c-iv at a pH of 5.7. The explants can then be transferred to the new b-12c-iv medium every 10-120 days (usually every 21 days), with contaminated tubes that are discarded. The contaminated tubes can be identified by bacterial discoloration of the agar or by visible surface contamination. These explants can remain in the b-12c-iv medium for 10-120 cycles per day (usually 21 cycles per day). The explants can then be removed from the medium after the third cycle if multiplication is occurring. If multiplication does not occur or is not occurring to a significant degree, the explants can be left in the middle for a fourth cycle.

The live shoots can then be transferred to a Stage 2 medium, such as b-9, CW1, CW2, CW3, CW4, CW5, CW6 or b-6 at a pH of 5.7. The cultures can remain in this Stage 2 medium until the desired number of shoots is obtained by separation in new tubes and additional expansion. Generally, the time interval includes 10-120 cycles per day (usually 14-21 cycles per day) between which crops are assigned to pass through another round of multiplication in the middle of Stage 2 or transition to a medium of Step 3, for example, b-10-iv or b-11-iv at a pH of 5.7 for further multiplication. One-ten shoots per tube can be obtained per multiplication cycle.

After the completion of the multiplication process, the shoots can be transferred to small tissue culture boxes (known as "magenta boxes") for 10-120 days (usually 14-21 days) containing a Stage 3 or Stage medium. 4, in this Example, BR2 at a pH of 5.7 for 10-120 days (generally 14-21 days) or Amel at a pH of 5.7 for 10-120 days (generally 14-21 days).

The following procedures may also be used (means of Stage 1, Stage 2, Stage 3, etc. are defined elsewhere herein): Starting with a bamboo plant between the ages of 3 months and 3 years, a node of the cane with the lateral bud that recently broke the sheath can be used as the explant. Each nodal section can be cut into sections of 3-5 μm with the shoot intact. Some explants, including explants taken from reeds 1 year old or older, can be pre-irrigated by shaking them in a 70% alcohol sopropil for 3 seconds, rinsing them under running water for 1 minute. Other explants are not pre-washed.

The outer sheaths can be peeled off and discarded and the remaining nodal part of the section placed in a 1% bleaching solution. The explant in bleaching solution can be placed on a vibrating table with Barnstead / Lab orbital linear agitator, adjustable speed, with laboratory rotators, (model number KS 260) for 1 hour at 6-9 revolutions per minute. For some implants, including those taken from reeds 1 year old or older, this stage can be modified by adding a few drops of Tween 20 to 1 0% bleaching solution and moistening the explants for 45 minutes after 1 hour. The explants can then be placed in 1% bleaching solution, and placed on the vibrating table again for 30 minutes. This 1% blotting solution step was then repeated.

The individual explants can then be placed in a Stage 1 medium (1 5-25 ml) inside a tube and the tubes placed in a clean growth chamber set at a temperature of 36.08 ° C-38.85 ° C (65 ° F). -70 ° F) and a light level spectral complete of 60-1-50 spark plug-meters (200-500 spark plug-feet). The medium of Step 1 can be b-12c-iv at a pH of 5.7. The explants can be transferred to the new b-12c-iv medium every 10-120 days (usually every 21 days), with contaminated tubes that are discarded. These explants can remain in the b-12c-iv medium for 10-120 cycles per day (usually 21 cycles per day). Between cycles, the excess pods can be eliminated. At the time of transfer to the third cycle, the explants can be changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. Following the third cycle, the explants can be cleaned. Explants can be maintained on b-12-c supplemented with 7 g / l of carrageenan instead of 5.5 g / l provided earlier for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks are observed. The observation of multiple outbreaks can occur within 3-15 months.

Once the explants exhibit multiple buds, it can be maintained in its Stage 2 medium or transferred to the Stage 3 medium. The non-limiting Stage 3 medium includes, a b-9 medium, a CW1 medium, a CW2 medium, a medium CW3, a CW4 medium, a CW5 medium, a CW6 medium or a b-6 medium at a pH of 5.7. The cultures may remain in a Stage 2 or Stage 3 medium until the desired number of shoots is obtained by separation in new tubes and additional expansion. - Generally, the time interval includes10-120 cycles per day (usually 21 cycles per day) between which crops can be assigned to pass through another round of multiplication or switched to a means of Stage 3 or Stage 4, such as a BR2 medium at a pH of 5.7 for 10-120 days (usually 21 days) in "magenta boxes" or an Amel medium at a pH of 5.7 for 10-120 days (usually 14-21 days).

In yet more particular non-limiting modes, the following species can be micropropagated into the following medium according to the procedures described in the preceding paragraphs, generally as a Step 2 medium, at a pH of 5.5-5.7: . Arundinaria gigantea: b-9- ?, CW1-V, CW3-v, CW4-V, CW5-v or CW6-v; Bambusa balcoa: b-9- ?, CW1-v, CW3-v, CW4-v, CW5-V or CW6-v; Bambusa vulgaris: b-9- ?, CW1-V, CW3-V, CW4-v, CW5-v or CW6-v; Bambusa vulgaris 'Vitatta': b-9- ?, CW1-v, CW3-V, CW4-V, CW5-v or CW6-v; Bambusa Oldhamü: b-9- ?, CW1-V, CW3-V, CW4-V, CW5-V or CW6-v; Bambusa tulda: b-9- ?, CW1-v, CW3-V, CW4-v, CW5-V or CW6-v; Dendrocalamus brandesii: b-9-v, CW1-v, CW3-v, CW4-v, CW5-v o.CW6-v; Dendrocalamus asper: b-9- ?, CW1-V, CW3-v, CW4-V, CW5-v or CW6-v; Dendrocalamus hamiltoni: b-9- ?. CW1-v. CW3-V, CW4-v, CW5-v or CW6-v; Dendrocalamus giganteus: b-9- ?, CW1-V, CW3-V, CW4-V, CW5-v or CW6-v; Dendrocalamus membranaceus: b-9- ?, CW1-v, CW3-v, CW4-v, CW5-V or CW6-v; Dendrocalamus strictus: b-9- ?, CWI-v, CW3-V, CW4-V, CW5-v or CW6-V.

Rough gigantochloa: b-9- ?, CW1-v, CW3-v, CW4-v, CW5-V or CW6-v; Gigantochloa scortechini: b-9- ?, CWI-v, CW3-v, CW4-V, CW5-v or CW6-v; Guadua culeata: b-9- ?, CWI-v, CW3-V, CW4-V, CW5-V or CW6-v; Guadua aculeata 'Nicaragua': b-9- ?, CW1-v, CW3-v, CW4-v, CW5-V or CW6-v; Guadua amplexifolia: b-9- ?, CW1-v, CW3-V, CW4-v, CW5-v or CW6-v; Guadua angustifolia: b-9- ?, CW1-v, CW3-v, CW4-V, CW5-V or CW6-v; Guadua angustofolia bl-color: b-9- ?, CW1-v, CW3-V, CW4-v, CW5-V or CW6-v; Guadua paniculata: b-9- ?, CW1-v, CW3-v, CW4-v, CW5-v or CW6-v; Melocanna bambusoides: b-9- ?, CWI-v, CW3-v, CW4-V, CW5-v or CW6-v; Neohouzeaua dullooa (Teinostachyum): b-9- ?, CW1-V, CW3-V, CW4-V, CW5-v or CW6-v; Ochlandra travancorica: b-9- ?, CW1-V, CW3-V, CW4-V, CW5-v or CW6-v; Phyllostachys edulis 'Moso': b-9- ?, CW1-v, CW3-V, CW4-v, CW5-v or CW6-v; Phyllostachys nigra: b-9- ?, CW1-V, CW3-V, CW4-V, CW5-V or CW6-v; Phyllostachys nigra 'Henon': b-9- ?, CW1-V, CW3-V, CW4-V, CW5-v or CW6-v; Schizostachyum lumampao: b-9-v, CW1-v, CW3-V, CW4-V, CW5-v or CW6-V.

As previously indicated, there are many uses for bamboos produced according to the methods described herein. In addition to or complement these uses described elsewhere, non-limiting examples of uses and products made from bamboos produced in accordance with the means, systems and methods described herein include: Exemplary paper types: Free sheet; Archive; Without acid; A4; Board; Bond; Book; Bristol board; Without carbon; Catalogue; Coated; Cover; Double purpose bond; Duplex; Finishing of English; Equivalent; Fine; Free sheet; Long Grain; Short Grain; Groundwood; Kraft; Light; News printing; Publication; Rag; Recycling; Label; Without cover; Virgin; Absorbent; Acid; Album; Albumin; Alkaline; Bank bill; Tissue; Dressing table; Towels; Fluff; File of cards; One-time carbon (OTC); optical character recognition (OCR); Tissue coating; and napkins.

Exemplary Pulp Types: Air-dried; Alpha; Bamboo; Bisulfate; Sulphite; Bleached; Chemical cellulose (diluted); Fluff; Forage; Free; Fully bleached; Hard; High alpha cellulose; Groundwood; Hot groundwood; Jute; Knotter; Kraft; Long fiber; Packed; Rolled up; Market; Without wood; Board; Groundwood pressure;; Rag; Recycling; Reinforced; Secondary; Semi-alkaline; Semi-bleached; Semi-chemical; Fiber short; Soda; Specialty; Sulfate; Thermochemical; Without bleaching; Viscous; and wood.

Board / Containers: Coated board; container board; Cardboard; old corrugated containers (OCC); and cardboard.

Wood: Structural Wood Panels (including structural laminated wood; oriented filament board; structural composite panels); Laminated laminated wood; structural composite wood (including laminated veneer wood; parallel filament wood; oriented filament wood9); I-Beams in I of prefabricated wood; floor junction; railway junctions; Floor; and compounds (including cars; Aircraft; Musical).

Textiles: Raw material; Filament yarn; Knitting; point; Narrow width fabric; Nonwoven; Spinning Yarn; Woven fabric; Viscous rayon; Wadding; Fiber removed; and cloth.

Textile Products: Clothing; Towels; Leaves / beds; Pillows; Curtains Food Sources: Sprouts; and any direct or bi-product for consumption of animal and human food.

Consumer goods: animal feed; Carpeted; Light bulbs; Household cleaning products; Chopsticks and toothpicks; Cleaning brooms; Bikes; Wheel chair; Fishing rods; Beer; Liqueur; Pharmaceutical products; Cosmetics; Soap / shampoo; Cookware; Arts; Furniture; Nutraceuticals; Paper cups; Paper plates; and diapers.

Energy and Bioenergy: Charcoal; Isolation; Raw material; and biomass.

The following non-limiting examples are provided. In all examples, the time in the medium that supports the transition to ex vitro conditions may be in air-permeable or air-impermeable containers. The described means are used in their solid forms provided above unless otherwise indicated differently as liquid.

EXAMPLES Example 1. Phyliostachys blssetti Starting with a bamboo plant between the ages of 3 months and 3 years, a node of the cane with the lateral bud that recently broke the pod can be used as the explant. Each nodal section was cut into sections of 3-5 millimeters with the shoot intact. The outer sheaths were peeled and discarded and the remaining nodal section piece was placed in 10% bleaching solution with a final concentration of 0.6% sodium hydrochlorides. The explant in bleaching solution was placed on a vibrating table with orbital shaker line Barnstead / Lab, adjustable speed, with laboratory rotators, (model number KS 260) for 1 hour at 6-9 revolutions during minute. The explants were then placed in 1% bleaching solution with a final concentration of 0.06% sodium hydrochloride, and placed on the vibrating table again for 30 minutes. This 1% bleach solution step was then repeated.

Individual explants were then placed in the medium of Step 1 (15-25 ml) inside a tube and the tubes were placed in a clean growth chamber set at a temperature of 36.08 ° C-38.85 ° C (65 ° F). 70 ° F) and a complete spectral light level of 60-150 spark-meters (200-500 spark plug-feet). The initial stage 1 medium in this Example was b-12c-iv at a pH of 5.7. The explants were transferred to the fresh b-12c-iv medium every 10-120 days (usually every 2.1 days), with the contaminated tubes discarded. The contaminated tubes were identified by bacterial discoloration of the agar or by visible surface contamination. These explants remained in medium b-12c-iv for 3-4 10-120 cycles per day (usually 21 cycles per day). The explants were taken from the medium after the third cycle if multiplication occurred. If the multiplication did not occur or occurred to a significant degree, the explants were left in the middle for a fourth cycle.

The live shoots were then transferred to a Stage 2 medium, in this Example, b-9-iv at a pH of 5.7. The cultures remained in medium b-9-iv until the desired number of shoots was obtained by separation in new tubes and further expansion. Generally, the time interval included 10-120 cycles per day (usually 14-21 cycles per day) between which the cultures were allocated to pass through another round of multiplication in the middle of Stage 2 or to switch to medium. Step 3, in this example, b-10-iv at a pH of 5.7 for further multiplication. One-ten shoots per tube were obtained by multiplication cycle.

After withdrawal from the multiplication process, the shoots were transferred to small tissue culture boxes (known as "magenta boxes") for 10-120 days (usually 14-21 days) that contained a Stage 3 or Stage 4 medium , in this Example BR-2-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 2. Fargesia denudata In the Fargeria denudata example, the explants were chosen and disinfected as in Example 1.

The explants were then transferred into jars containing the medium of Step 1, in this Example, b-12c-iv (liquid, 30-40 ml) as described in Example 1 but for the use of rockrose. The explants were transferred to the medium after the third cycle if multiplication occurred. If the multiplication did not occur or occurred to a significant degree, the explants were left in the middle for a fourth cycle. The contaminated tubes were discarded.

The cultures were then transferred onto a Stage 2 medium, in this example, b-11-iv (liquid) in jars on a rotating rack providing 6-9 revolutions per minute. The cultures remained in medium b-11-iv at a pH of 5.7 for 10-120 cycles per day (usually 14 cycles per day) until the desired number of shoots was obtained by jar separation and further expansion. One-fifteen shoots per jar were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this Example, Ech-iv at a pH of 6 for 10-120 days (usually 14-21 days).

Example 3. Pleioblastus fortunei In the example of Pleioblastus fortune !, the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 1. The shoots were then transferred to a Stage 2 medium, in this Example, b-9-iv in the magenta boxes (40-50 ml). They remained in medium b-9-iv for 10-120 cycles per day (usually 14 cycles per day) until the desired number of shoots was obtained by separation in new boxes and additional expansion. One-twenty shoots per box were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this example, BR-2-iv for 10-120 days (usually 14-21 days). Example 4. Sasa Veitchli In the example of Sasa Veitchü, the explants were chosen and disinfected as in Example 1.

The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 1. The shoots were then transferred in a medium of Step 2, in this example, b -1-iv at a pH of 5.5 for 10-120 cycles per day (usually 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this example, Br-2-iv at a pH of 5.7 for 14-21 days.

Example 5. Pleioblastus viridistriatus and Thamnocalamus crassinodus In the example of Pleioblastus viridistriatus and Thamnocalamus crassinodus, the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 1. The brot.es were then transferred in a medium of Stage 2, in this Example, b-4-iv to a pH of 5.5 for 10-120 cycles per day (generally 21 cycles per day) until the number Desired outbreaks were obtained by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by cycle of multiplication. The shoots were then placed in a Stage 3 medium, in this Example, Br-2-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 6. Chusquea Culeo "Cana Prieta" In the Chusquea Culeo Example "Cana Prieta", the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, the medium b-12c-v also as described in Example 1. The shoots were then transferred in a medium of Step 2, in this Example, b-9-iv to a pH of 5.5 for 10-120 cycles per day (generally 21 cycles per day) until The desired number of shoots was obtained by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days). Example 7. Bambusa Old Hamii In the Bambusa Oíd Hamii Example, the explants were chosen and disinfected as in Example 1. The explants were then transferred in boxes containing a Stage 1 medium, in this Example, b-10-iv (40-50 ml) ) also as described in Example 1 but for the change to boxes. The shoots were maintained in medium b-10-iv for 10-120 cycles per day (usually 21 cycles per day) until the desired number of shoots was obtained by separation in new boxes and additional expansion. One-twenty shoots per box were obtained by multiplication cycle. The shoots were then placed in a Stage 2 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 8. Phyllostachys Edulis "Moso", Phyllostachys Atrovaginata & Dendrocalamus Asper In the example of Phyllostachys Edulis "Moso", Phyllostachys Atrovaginata & Dendrocalamus Asper, the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 1. The shoots then were transferred in a Step 2 medium, in this Example, b-9-iv to a pH of 5.5 for 10-120 cycles per day (usually 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and additional expansion. A medium B-6 at a pH of 5.5 can also be used. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 9. Guadua Angustí folia In the Guadua Angustofolia Example, the explants were chosen and disinfected as in Example 1. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 1 The shoots were then transferred in a medium of Step 2, in this Example, b-10-iv to a pH of 5.5 for 10-120 cycles per day (usually 21 cycles per day) until the desired number of shoots was obtained. by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 10. Phyllostachys bissetti - alternative procedure Starting with a bamboo plant between the ages of 3 months and 3 years, a node of the cane with the lateral bud that recently broke the pod can be used as the explant. Each nodal section was cut into sections of 3-5 millimeters with the shoot intact. Some explants, including explants taken from reeds 1 year old or older, were pre-rinsed by shaking them in a 70% isopropyl alcohol jug for 3 seconds by rinsing them under running tap water for 1 minute. Other explants were not pre-rinsed. neither The outer sheaths were peeled off and discarded and the remaining nodal section piece was placed in 10% bleaching solution. The explant in the bleaching solution was placed on an agitator table with Barnstead / Lab orbital shaker, adjustable speed, with laboratory rotators, (model number KS 260) for 1 hour at 6-9 revolutions per minute. For some implants, including those taken from reeds 1 year or older, this stage was modified by adding a few drops of Tween 20 to 10% bleaching solution and moistening the explants for 45 minutes instead of 1 hour. The explants were then placed in 1% bleaching solution, and placed again on the shaking table for 30 minutes. This 1% solution of bleaching stage was then repeated.

Individual explants were then placed in a Stage 1 medium (15-25 ml) inside a tube and the tubes were placed in a clean growth chamber set at a temperature of 36.08 ° C-38.85 ° C (65 ° F). 70 ° F) and a complete spectral light level of 60-150 spark-meters (200-500 spark plug-feet). In this Example, the medium of Step 1 was b-12c-iv at a pH of 5.7. The explants were transferred to the fresh b-12c-iv medium every 10-120 days (usually every 21 days), with contaminated tubes that were discarded. These explants remained in a b-12c-iv medium for 2 10-120 cycles per day (usually 21 cycles per day). Between the cycles, the excess pods were eliminated. At the time of transfer to the third cycle, the explants were transferred to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / L of carrageenin for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple shoots, it was maintained in its Stage 2 medium or transferred to a Stage 3 medium, in this Example, when b-9-iv was used at a pH of 5.7. Alternatively using one of the B-9 medium, CW1 medium at a pH of 5.7 can also be used. The cultures remained in the medium of Stage 2 or Stage 3 until the desired number of shoots was obtained by separation in new tubes and further expansion. Generally, the time interval included 10-120 cycles per day (usually 21 cycles per day) between which the cultures were allocated to pass through another round of multiplication or switched to a Stage 3 or Stage 4 medium, in this Example, BR-2-iv at a pH of 5.7 for 10-120 days (usually 21 days) ~ in "magenta boxes".

Example 11. Fargesia denudata · alternative procedure In the Fargeria denudata Example, the explants were chosen and disinfected as in Example 10. The explants were then transferred into jars containing a Stage 1 medium, in this Example, b-12c-iv (liquid; 30-40 mi) as described in Example 10 but for the use of jars. These explants remained in a b-12c-iv medium for 2 10-120 cycles per day (usually 21 cycles per day). Between the cycles, the excess pods were eliminated. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. Following the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, it was maintained in its Stage 2 medium or transferred to a Stage 3 medium, in this Example, b-11-iv (liquid) at a pH of 5.7 in jars on a rotating shelf that provides 6-9 revolutions per minute. The cultures remained in the medium of Stage 2 or Stage 3 for 10-120 cycles per day (generally 14 cycles per day) until the desired number of shoots was obtained by separation in new jars and further expansion. One-fifteen shoots per jar were obtained per multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, Ech-iv at a pH of 6 for 10-120 days (generally 21 days).

Example 12 Pleioblastus fortune] - alternative procedure In the Pleioblastus fortunei Example, the explants were chosen and disinfected as in Example 10. The explants then they were transferred into tubes containing a Stage 1 medium in this Example, b-12c-iv also as described in Example 10. These explants remained in medium b-12c-v for 2 10-120 cycles per day (usually 21 cycles per day). Between the cycles, the excess pods were eliminated. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, it was maintained in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-9-iv in magenta boxes (40-50 ml), (CVV1 medium can also be used ). The medium b-9-iv remained for 10-120 cycles per day (usually 14 cycles per day) until the desired number of shoots was obtained by separation in new boxes and additional expansion. One-twenty shoots per box were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, BR-2-iv for 10-120 days (usually 14-21 days).

Example 13. Sasa Veitchü - alternative procedure In the Sasa Veitchü Example, the explants were chosen and disinfected as in Example 10.

The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 10. These explants remained in medium b-12c-iv for 2 10-120 cycles per day (usually 21 cycles per day). Between cycles, excess pods were removed. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple shoots, it was maintained in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-1-iv at a pH of 5.5 for 10-120 cycles per day (generally 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and one-ten additional expansion shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, Br-2-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 14. Pleioblastus viridistriatus and Thamnocalamus crassinodus - alternative procedure In the Example of Pleioblastus viridistriatus and Thamnocalamus crassinodus, the explants were chosen and disinfected as in Example 10. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 10. These explants remained in medium b-12c-iv for 2 cycles of 10-120 days (generally 21 cycles per day). Between cycles, excess pods were removed. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, or was kept in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-4-iv at a pH of 5.5 for 10-120 cycle per day (generally 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, Br-2-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 15. Chusquea Culeo "Cana Prieta" - alternative procedure In the Chusquea Culeo Example "Cana Prieta", the explants were chosen and disinfected as in Example 10. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 10. These explants remained in a medium b-12c-iv for 2 10-120 cycles per day (generally 21 cycles per day). Between cycles, excess pods were removed. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, or was kept in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-9-iv at a pH of 5.5 for 10-120 cycles per day (generally 21 days) until the desired number of shoots was obtained by separation in new tubes and additional expansion. The B-6 medium at a pH of 5.5 can also be used. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, the Amel-iv medium at a pH of 5.7 for 10-120 days (generally 14-21 days).

Example 16. Bambusa Oíd Hamli - alternative procedure In the Bambusa Oíd Hamii Example, the explants were chosen and disinfected as in Example 10. The explants were then transferred in boxes containing a Stage 1 medium, in this Example, b-10-iv (40-50 ml) also as described in Example 10 but for the change to boxes. These explants remained in a b-10-iv medium for 2 cycles of 10-120 days (generally 21 cycles per day). Between cycles, excess pods were removed. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-10-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-10-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months. The cultures were maintained in a Stage 2 medium until the desired number of shoots was obtained. One-twenty shoots per box were obtained by multiplication cycle. The shoots were then placed in a Stage 3 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 17. Phyllostachys Moso, Phyllostachys Atrovaginata & Dendrocalamus Asper - alternative procedure In the Example of Phyllostachys Moso, Phyllostachys Atrovaginata & Dendrocalamus Asper, the explants were chosen and disinfected as in Example 10. The explants were then transferred into tubes containing a Stage 1 medium, in this Example, b-12c-iv also as described in Example 10. These explants they remained in a medium b-12c-iv for 2 cycles of 10-120 days (usually 21 cycles per day). Between the cycles, the excess pods were eliminated. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, or was kept in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-9-iv at a pH of 5.5 for 10-120 cycles per day (generally 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and additional expansion. The B-6 medium at a pH of 5.5 can also be used. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, Amel-iv at a pH of 5.7 for 10-120 days (usually 14-21 days).

Example 18 Guadua Angustifolia - alternative procedure In the Guadua Angustofolia Example, the explants were chosen and disinfected as in Example 10. The explants were then transferred into tubes containing a Step 1 medium, in this Example, b-12c-iv also as described in Example 10. These explants remained in medium b-12c-v for 2 cycles of 10-120 days (generally 21 cycles per day). Between cycles, the excess pods were eliminated. At the time of transfer to the third cycle, the explants were changed to a Stage 2 medium, in this Example, b-12-c supplemented with 7 g / L of carrageenan instead of 5.5 g / L provided above. After the third cycle, the explants were cleaned. The explants were maintained in b-12-c supplemented with 7 g / l of carrageenan for 10-120 cycles per day (usually 21 cycles per day) until multiple outbreaks were observed. The observation of multiple outbreaks occurred within 3-15 months.

Once the explant exhibited multiple buds, or was maintained in its Stage 2 medium or transferred to a Stage 3 medium, in this Example b-10-iv at a pH of 5.5 for 10-120 cycles per day (generally 21 cycles per day) until the desired number of shoots was obtained by separation in new tubes and additional expansion. One-ten shoots per tube were obtained by multiplication cycle. The shoots were then placed in a Stage 3 or Stage 4 medium, in this Example, Amel-iv at a pH of 5.7. for 10-120 days (usually 14-21 days).

As will be understood by one skilled in the examples provided, the tissue culture method for the individual species includes slight variations in medium, duration and growth conditions. These variations for individual species require optimization based on factors that include location, desired result, raw material, etc.

For each of the species provided in the Examples listed above, in the particular modalities, each can be generated and / or multiplied in a medium b-9-i, medium b-9-ii, medium b-9-iii, medium b-9 - ??, medium b-9- ?, medium. CW2-¡, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10- iv, medium b-10- ?, medium b-11-i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b-12c-i , medium b - 12 c - ii, medium b - 12c - üi, medium b - 12c - iv, medium b - 12c - v, medium b - 1 - i. medium b-1-ii, medium b-1-i¡¡, medium b-1-iv, medium b-1- ?, medium b-4-i, medium b-4-ii, medium b-4-iii , medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-ii, medium b-6-üi, medium b-6 - ??, medium b-6-? , measured CW1-Í, medium CW1-Ü, medium CW1-¡¡¡, medium CW1-iv, medium CW1-v, medium CW3-i, medium CW3-Ü, medium CW3-MÍ, medium CW3-iv, medium CW3 -V, medium CW4-,, medium CW4-Ü, medium CW4-ii¡, medium CW4-iv, medium CW4-v, medium CW5-,, medium CW5-M, medium CVV5-Ü, medium CW5-,, medium CW5-V, CW6-Í medium, CW6-Ü medium, CW6-iii medium, CW6-medium and CW6-v medium.

As used in the present "in" and "on" are interchangeable in the context of placing explants, shoots or seedlings inside a tube, jar, box or jar containing the medium.

Unless otherwise indicated, all numbers expressing the ingredient quantities, characteristics such as molecular weight, reaction conditions, and so forth used in the specification and claims shall be understood as modified in all cases by the term " approximately". Accordingly, unless otherwise indicated, the numerical parameters set forth in the specification and appended claims are approximations that may vary depending on the desired characteristics intended to be obtained by the present invention. At least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter must at least be interpreted in light of the number of significant digits described and applying ordinary rounding techniques.

Although the numerical ranges and parameters that establish the broad scope of the invention are approximations, the numerical values set forth in the specific Examples are reported as accurately as possible. Any numerical value, however, intrinsically contains certain errors that necessarily result from the standard deviation found in their respective test measurements.

The terms "one", "ones", "the" and similar referents used in the context describing the invention (especially in the context of the following claims) should be construed as including the singular and plural terms, unless Indicate otherwise in the present or be contradicted clearly by the context. The mention of the ranges of values in the present is simply intended to serve as an abbreviated method to refer individually to each separate value that lies within the range. Unless otherwise indicated in this, each individual value is incorporated in the specification as if it were mentioned individually in the present. All methods described herein may be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by the context. The use of any and all examples, or exemplary language (eg, "such as") provided herein is intended merely to clarify the invention better and does not raise a limitation on the scope of the claimed invention otherwise. No language in the specification should be interpreted as an indication of any unclaimed element essential to the practice of the invention.

The groupings of alternative elements or embodiments of the invention described herein are not to be construed as limitations. Each member of the group can refer and claim individually or in any combination with other members of the group or other elements found in this. It is anticipated that one or more members of a group may be included in, or removed from, a group for reasons of convenience and / or patentability. When any inclusion or deletion occurs, the specification is judged to contain the group as modified thus complying with the written description of all the Markush groups used in the appended claims.

Certain embodiments of this invention are described herein, including the best mode known to the inventors for carrying out the invention. By placing, variations to these described modalities will become apparent to those skilled in the art through reading the above description. The inventor expects that those skilled in the art will use such variations as appropriate, and the inventors claim that the invention is practiced otherwise than as specifically described herein. Accordingly, this invention includes all modifications and equivalents of the subject matter cited in the claims appended thereto as permitted by applicable law. On the other hand, any combination of the elements described above in all possible variations thereof is encompassed by the invention unless otherwise indicated to the contrary herein or otherwise clearly contradicted by the context.

The specific embodiments described herein may be further limited in the claims by using the language to consist of and / or consisting essentially of. When used in the claims, whether as presented or added by the amendment, the transition term "consisting of" excludes any element, step, or ingredient not specified in the claims. The transition term "consisting essentially of" limits the scope of a claim to the materials or steps specified and to those that do not materially affect the basic and new characteristics. The embodiments of the invention thus claimed are described and permitted intrinsically or expressly herein.

In conclusion, it should be understood that the embodiments of the invention described herein are illustrative of the principles of the present invention. Other modifications that may be used are within the scope of the invention. Thus, by way of example, but not limitation, the alternative configurations of the present invention may be used in accordance with the teachings herein. Accordingly, the present invention is not limited thereto exactly as shown and described.

Claims (19)

1. A means for micropropagation of bamboo, wherein the medium comprises meta-topolin or an analogue thereof and supports 10-120 multiplication cycles per day for at least six months.

2. A medium according to claim 1, wherein the medium supports 10-120 multiplication cycles per day for at least one year.

3. A medium according to claim 1 or 2, wherein the meta-topolin or analogue thereof is present in an amount of 0.0125 mg / ml-10 mg / ml.

4. A medium according to claim 1, 2 or 3, wherein the medium additionally comprises thidiazuron or an analogue thereof.

5. A medium according to claim 1, 2, 3, or 4 wherein the medium additionally comprises NAA, BAP, 2 i p and IBA.

6. A method for the micropropagation of bamboo, "comprising cultivating bamboo explants and / or shoots in the middle of claim 1, 2, 3, 4 or 5.

7. A method for the micropropagation of bamboo according to claim 7, wherein the bamboo is Phyllostachys bissetti; Fargesia denudata; Pleioblastus fortunei; Sasa Veitchii; Pleioblastus viridistriatus; Thamnocalamus crassinodus; Chusquea Culeo "Cana Prieta"; Bambusa Old Hamii; Phyllostachys bear; Phyllostachys Atrovaginata; Dendrocalamus Asper; or Guadua Angustifolia.

8. A method for micropropagation of bamboo according to claim 7, wherein the bamboo is Arundinaria gigantea; Bambusa balcoa; Bambusa vulgaris; Bambusa vulgaris 'Vitatta'; Bambusa Oldhamii; Bambusa tulda; endrocalamus brandesii; Dendrocalamus asper; Dendrocalamus hamiltoni; Dendrocalamus giganteus; Dendrocalamus membranaceus; Dendrocalamus strictus; Rough gigantochloa; Gigantochloa scortechini; Guadua culeata; uadua aculeata 'Nicaragua'; Guadua amplexifolia; Guadua angustifolia; Guadua angustofolia bi-color; Guadua paniculata; Melocanna bambusoides; eohouzeaua dullooa (Teinostachyum); Ochlandra travancorica; Phyllostachys edulis 'Moso'; Phyllostachys nigra; Phyllostachys nigra 'Henon'; or Schizostachyum lumampao.

9. A means for the transition of shoots to ex-vi conditions, where the medium comprises, consists essentially of or consists of the medium Br-2-i, medium Br-2-ii, medium Br-2-iii, medium Br- 2-iv, half Ech-i, half Ech-ii, half Ech-iii, half Ech-iv, half Amel-i, half Amel-ü, half Amel-iii, half Amel-iv or Amel-v.

10. A means for micropropagation of bamboo, wherein the medium comprises thidiazuron or an analogue thereof and supports 10-120 cycles of multiplication per day for at least six months.

11. A medium according to claim 10, wherein the medium supports 10-120 multiplication cycles per day for at least one year.

12. A medium according to claim 10 or 11, wherein the thidiazuron or analogue thereof is present in an amount of 0.0001 mg / ml-5 mg / ml.

13. A medium according to claim 10, 11 or 12, wherein the medium additionally comprises meta-topolin or an analogue thereof, NAA, BAP, 2ip and IBA.

14. A medium according to claim 13, wherein the meta-topolin or analogue thereof is present in an amount of 0.0125 mg / ml-10 mg / ml.

15. A medium according to claim 1, 2, 3, 4, 5, 10, 11, 12, 13 or 14, wherein the medium comprises, consists essentially of or consist of a medium b-9-i, medium b- 9-ii, medium b-9-iii, medium b-9-iv, medium b-9- ?, medium CW2-Í, medium CW2-Ü, medium CW2-iii, medium CW2-iv, medium CW2-v, medium b-10-i, medium b-10-ii, medium b-10-iii, medium b-10-iv, medium b-10- ?, medium b-11 -i, medium b-11-ii, medium b-11-iii, medium b-11-iv, medium b-11- ?, medium b- 2c-i, medium b-12c-ii, medium b-12c-iii, medium b-12c-iv, medium b-12c-v, medium b-1-i, medium b-1-ii, medium b-1-iii, medium b- -iv, medium b-1-, medium b-4-i, medium b- 4-ii, medium b-4-iii, medium b-4-iv, medium b-4-, medium b-6-i, medium b-6-i, medium b-6-üi, medium b- 6-iv. medium b-6- ?. medium CW1-L medium CW1-Ü, medium CW1-iii, medium CW1-iv, medium CW1-v, medium CW3-i, medium CW3-Ü, medium CW3-i¡¡, medium CW3-iv, medium CW3-V, medium CW4-Í, medium CW4-ii, medium CW4- i¡¡, half CW4-iv, half CW4-V, half CW5-i, half CW5-Ü, half CW5-ii¡, half CW5-v, half CW5-V, half CW6-i, half CW6-Ü , CW6-ME medium, CW6-iv medium and / or CW6-V medium.

16. A method for bamboo micropropagation comprising cultivating bamboo explants and / or shoots in the medium of claim 11, 12, 13, 14 or 15.

17. A method for micropropagation of bamboo according to claim 16, wherein the bamboo is Phyllostachys bissetti; Fargesia denudata; Pleioblastus fortunei; Sasa Veitchü; Pleioblastus viridistríatus; Thamnocalamus crassinodus; Chusquea Culeo "Cana Prieta"; Bambusa Old Hamii; Phyllostachys Moso; Phyllostachys Alrovaginata; Dendrocalamus Asper; or Guadua Angustifolia.

18. A method for micropropagation of bamboo according to claim 7, wherein the bamboo is Arundinaria gigantea; Bambusa balcoa; Bambusa vulgaris; Bambusa vulgaris 'Vitatta'; Bambusa Oldhamii; Bambusa tulda; endrocalamus brandesii; Dendrocalamus asper; Dendrocalamus hamiltoni; Dendrocalamus giganteus; Dendrocalamus membranaceus; Dendrocalamus strictus; Rough gigantochloa; Gigantochloa scortechini; Guadua culeata; uadua aculeata 'Nicaragua'; Guadua amplexifolia; Guadua angustifolia; Guadua angustofolia b-color; Guadua paniculata; Melocanna bambusoides; eohouzeaua dullooa (Te'mostachyum); Ochlandra travancorica; Phyllostachys edulis 'Moso'; Phyllostachys nigra; Phyllostachys nigra 'Henon'; or Schizostachyum lumampao.

19. A kit comprising a medium according to any of claims 1, 2, 3, 4, 5, 9, 10, 11, 12, 13, 146 15.