MX2009004985A - Secuencias para activar la expresión y control de las modificaciones de post-traducción de un polipéptido recombinante. - Google Patents
Secuencias para activar la expresión y control de las modificaciones de post-traducción de un polipéptido recombinante.Info
- Publication number
- MX2009004985A MX2009004985A MX2009004985A MX2009004985A MX2009004985A MX 2009004985 A MX2009004985 A MX 2009004985A MX 2009004985 A MX2009004985 A MX 2009004985A MX 2009004985 A MX2009004985 A MX 2009004985A MX 2009004985 A MX2009004985 A MX 2009004985A
- Authority
- MX
- Mexico
- Prior art keywords
- polypeptide
- post
- recombinant
- recombinant polypeptides
- present
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 15
- 229920001184 polypeptide Polymers 0.000 title abstract 14
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 14
- 230000008685 targeting Effects 0.000 title abstract 2
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 4
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 230000005847 immunogenicity Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 230000004481 post-translational protein modification Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8221—Transit peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
La presente invención proporciona nuevas herramientas para controlar las modificaciones de post-traducción de polipéptidos recombinantes. Estas herramientas son péptidos de señal particulares que permiten la activación de polipéptidos recombinantes durante su síntesis en una célula huésped para compartimientos sub-celulares específicos y un diseño específico de dichos polipéptidos recombinantes dentro de dichos compartimientos sub-celulares. Estos péptidos de señal son SEC ID No. 1 a SEC ID No. 31 y se describen aquí. La presente invención se refiere, por lo tanto, también a un procedimiento para producir un polipéptido recombinante, en particular a un polipéptido modificado de manera de post-traducción, que comprende los pasos de transfectar o transformar una célula con al menos un vector de ácido nucleico que codifica una proteína recombinante, la cual es el polipéptido que antes fe modificado de manera de post-traducción o una proteína recombinante diferente a dicho polipéptido. La proteína recombinante comprendiendo una señal de péptido de acuerdo con la presente invención; desarrollar la célula transfectada; y cosechar el polipéptido modificado de manera post-traducción; en donde, cuando dicha proteína recombinante es diferente al polipéptido, el método también comprende un paso de transfectar dicha célula con por lo menos un vector que codifica dicho polipéptido. La presente invención permite ventajosamente, por ejemplo, incrementar el rendimiento de producción de un polipéptido recombinante, para evitar la inmunogenicidad de polipéptidos recombinantes y para obtener polipéptidos recombinantes terapéuticamente activos que la copia exacta de su contraparte natural. Esta invención se refiere particularmente al campo de reorientación de plantas hechas farmacéuticos (PMP).
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85752406P | 2006-11-08 | 2006-11-08 | |
| PCT/IB2007/004234 WO2008056265A2 (en) | 2006-11-08 | 2007-11-08 | A set of sequences for targeting expression and control of the post-translationnal modifications of a recombinant polypeptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2009004985A true MX2009004985A (es) | 2009-10-08 |
Family
ID=39364897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2009004985A MX2009004985A (es) | 2006-11-08 | 2007-11-08 | Secuencias para activar la expresión y control de las modificaciones de post-traducción de un polipéptido recombinante. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20110195452A1 (es) |
| EP (1) | EP2089526B1 (es) |
| JP (1) | JP5568702B2 (es) |
| KR (1) | KR20090079258A (es) |
| CN (1) | CN101622354A (es) |
| AU (1) | AU2007318926B2 (es) |
| BR (1) | BRPI0716711A2 (es) |
| CA (1) | CA2669455A1 (es) |
| IL (1) | IL198659A (es) |
| MX (1) | MX2009004985A (es) |
| RU (1) | RU2481399C2 (es) |
| WO (1) | WO2008056265A2 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013067322A1 (en) * | 2011-11-03 | 2013-05-10 | Stc Biologics, Inc. | Method for determination of pharmacological properties of recombinant proteins |
| FR2991996B1 (fr) | 2012-06-13 | 2016-07-08 | Angany Genetics | Methode de production d'allergenes recombinants de haute qualite par expression transitoire chez nicotiana benthamiana |
| FR3004462B1 (fr) | 2013-04-15 | 2016-04-01 | Angany Genetics | Production commerciale de peptidases c1a par expression transitoire chez les plantes |
| US9796757B2 (en) * | 2013-04-19 | 2017-10-24 | The Board Of Trustees Of The University Of Illinois | Compositions and method for treating thrombosis |
| FR3021328B1 (fr) | 2014-05-23 | 2018-01-05 | Angany Inc. | Production commerciale de l'allergene amb a1 par expression transitoire chez les plantes |
| EA038931B9 (ru) * | 2014-11-20 | 2022-02-18 | Йиссум Рисерч Дивелопмент Компани Оф Зе Хебрю Юниверсити Оф Иерусалим Лтд. | Композиции и способы получения полипептидов с модифицированным профилем гликозилирования в клетках растений |
| FR3054547B1 (fr) | 2016-07-29 | 2020-06-05 | Angany Inc. | Particules pseudo-virales et leurs utilisations |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2252960C2 (ru) * | 1996-12-13 | 2005-05-27 | Зингента Партисипейшнс Аг | Ген, обусловливающий устойчивость растений к болезням, и его применение |
| WO2001029242A2 (en) * | 1999-10-21 | 2001-04-26 | Monsanto Company | Post-translational modification of recombinant proteins produced in plants |
| FR2808286B1 (fr) * | 2000-04-26 | 2004-12-17 | Agronomique Inst Nat Rech | Nouvelle glucosidase i de plante et son application a la production de proteines recombinantes a glycosylation modifiee |
| AU2003219418B2 (en) * | 2002-03-19 | 2008-02-28 | Stichting Dienst Landbouwkundig Onderzoek | Optimizing glycan processing in plants |
-
2007
- 2007-11-08 WO PCT/IB2007/004234 patent/WO2008056265A2/en active Application Filing
- 2007-11-08 US US12/513,947 patent/US20110195452A1/en not_active Abandoned
- 2007-11-08 EP EP07859279A patent/EP2089526B1/en active Active
- 2007-11-08 KR KR1020097011735A patent/KR20090079258A/ko not_active Application Discontinuation
- 2007-11-08 CN CN200780049458A patent/CN101622354A/zh active Pending
- 2007-11-08 MX MX2009004985A patent/MX2009004985A/es active IP Right Grant
- 2007-11-08 JP JP2009535834A patent/JP5568702B2/ja not_active Expired - Fee Related
- 2007-11-08 BR BRPI0716711-3A2A patent/BRPI0716711A2/pt not_active Application Discontinuation
- 2007-11-08 AU AU2007318926A patent/AU2007318926B2/en not_active Ceased
- 2007-11-08 CA CA002669455A patent/CA2669455A1/en not_active Abandoned
- 2007-11-08 RU RU2009120298/10A patent/RU2481399C2/ru not_active IP Right Cessation
-
2009
- 2009-05-07 IL IL198659A patent/IL198659A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP2089526B1 (en) | 2013-03-13 |
| CA2669455A1 (en) | 2008-05-15 |
| AU2007318926A1 (en) | 2008-05-15 |
| US20110195452A1 (en) | 2011-08-11 |
| AU2007318926B2 (en) | 2013-05-02 |
| WO2008056265A2 (en) | 2008-05-15 |
| IL198659A0 (en) | 2011-08-01 |
| JP5568702B2 (ja) | 2014-08-13 |
| IL198659A (en) | 2015-05-31 |
| KR20090079258A (ko) | 2009-07-21 |
| RU2481399C2 (ru) | 2013-05-10 |
| EP2089526A2 (en) | 2009-08-19 |
| CN101622354A (zh) | 2010-01-06 |
| WO2008056265A3 (en) | 2008-08-28 |
| RU2009120298A (ru) | 2010-12-20 |
| BRPI0716711A2 (pt) | 2013-10-01 |
| JP2010508839A (ja) | 2010-03-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |