MD957Z - Process for treating lettuce seeds - Google Patents
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- MD957Z MD957Z MDS20150068A MDS20150068A MD957Z MD 957 Z MD957 Z MD 957Z MD S20150068 A MDS20150068 A MD S20150068A MD S20150068 A MDS20150068 A MD S20150068A MD 957 Z MD957 Z MD 957Z
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- 238000000034 method Methods 0.000 title claims abstract description 16
- 235000003228 Lactuca sativa Nutrition 0.000 title claims description 22
- 241000208822 Lactuca Species 0.000 title abstract 2
- 240000008415 Lactuca sativa Species 0.000 claims abstract description 27
- 238000009331 sowing Methods 0.000 claims abstract description 9
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 239000000284 extract Substances 0.000 claims abstract description 6
- 229930182470 glycoside Natural products 0.000 claims abstract description 6
- 150000002338 glycosides Chemical class 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 244000247695 Veronica chamaedrys Species 0.000 claims abstract description 5
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 4
- 229920006395 saturated elastomer Polymers 0.000 claims abstract description 4
- 238000002791 soaking Methods 0.000 claims abstract description 4
- 238000000746 purification Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 12
- 229910052793 cadmium Inorganic materials 0.000 abstract description 11
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 abstract description 8
- 238000009825 accumulation Methods 0.000 abstract description 6
- 235000013311 vegetables Nutrition 0.000 abstract description 3
- 238000001035 drying Methods 0.000 abstract description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 8
- 229910052708 sodium Inorganic materials 0.000 description 8
- 239000011734 sodium Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 231100000674 Phytotoxicity Toxicity 0.000 description 2
- 235000000384 Veronica chamaedrys Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- HZUURLOSYMEMET-UHFFFAOYSA-N 106323-63-1 Natural products OC1C(O)C(O)C(C)OC1OC1C(OC2C(C(O)C(O)CO2)O)C(OCCC=2C=C(O)C(O)=CC=2)OC(CO)C1OC(=O)C=CC1=CC=C(O)C(O)=C1 HZUURLOSYMEMET-UHFFFAOYSA-N 0.000 description 1
- 244000024251 Aralia cordata Species 0.000 description 1
- HZUURLOSYMEMET-DAVUXQIISA-N Ehrenoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](O[C@H]2[C@@H]([C@@H](O)[C@@H](O)CO2)O)[C@H](OCCC=2C=C(O)C(O)=CC=2)O[C@H](CO)[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 HZUURLOSYMEMET-DAVUXQIISA-N 0.000 description 1
- 229930185474 acteoside Natural products 0.000 description 1
- FBSKJMQYURKNSU-ZLSOWSIRSA-N acteoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@@H](CO)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O FBSKJMQYURKNSU-ZLSOWSIRSA-N 0.000 description 1
- FBSKJMQYURKNSU-UKQWSTALSA-N acteoside I Natural products C[C@@H]1O[C@H](O[C@@H]2[C@@H](O)[C@H](OCCc3ccc(O)c(O)c3)O[C@H](CO)[C@H]2OC(=O)C=Cc4ccc(O)c(O)c4)[C@H](O)[C@H](O)[C@H]1O FBSKJMQYURKNSU-UKQWSTALSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000001479 atomic absorption spectroscopy Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- ZIEPAEMUJQKFRJ-UHFFFAOYSA-N ehrenoside Natural products CC1OC(OC2C(OC3OCC(O)C(O)C3O)C(OCCc4ccc(O)c(O)c4)OC(CO)C2OC(=O)C=Cc5cc(O)cc(O)c5)C(O)C(O)C1O ZIEPAEMUJQKFRJ-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 229930182489 iridoid glycoside Natural products 0.000 description 1
- 150000008145 iridoid glycosides Chemical class 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- UDHCHDJLZGYDDM-KBJPJAGZSA-N lavandulifolioside Natural products C[C@@H]1O[C@@H](O[C@@H]2[C@@H](O)[C@H](OCCc3ccc(O)c(O)c3)O[C@H](CO)[C@H]2OC(=O)C=Cc4ccc(O)c(O)c4)[C@H](O[C@@H]5OC[C@H](O)[C@H](O)[C@H]5O)[C@H](O)[C@H]1O UDHCHDJLZGYDDM-KBJPJAGZSA-N 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- QFRYQWYZSQDFOS-UHFFFAOYSA-N verbascoside Natural products CC1OC(COC2C(O)C(COC3OC(C(O)C(O)C3O)C(=O)O)OC(Oc4cc(O)cc5OC(=CC(=O)c45)c6ccc(O)c(O)c6)C2O)C(O)C(O)C1O QFRYQWYZSQDFOS-UHFFFAOYSA-N 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Description
Invenţia se referă la legumicultură, şi anume la un procedeu de tratare a seminţelor de salată verde Lactuca sativa L. înainte de semănat. The invention relates to vegetable growing, namely to a process for treating lettuce seeds Lactuca sativa L. before sowing.
Invenţia poate fi utilizată în tehnologia de cultivare a salatei verzi în zonele agricole cu risc înalt de poluare. The invention can be used in the technology of growing green lettuce in agricultural areas with high risk of pollution.
Este cunoscut procedeul de reducere a fitotoxicităţii metalelor grele prin tratarea seminţelor şi plantelor de salată verde cu soluţii apoase de 0,0175% şi 0,035% de gumat de sodiu, însă eficacitatea acestui procedeu este relativ redusă [1]. The process of reducing the phytotoxicity of heavy metals by treating lettuce seeds and plants with aqueous solutions of 0.0175% and 0.035% sodium gumate is known, but the effectiveness of this process is relatively low [1].
Dezavantajul procedeului dat constă în faptul că soluţia de gumat de sodiu se introduce în sol prin irigare în cantităţi relativ mari (300 L/ha); procedeul diminuează fitotoxicitatea metalelor grele, dar nu reduce tendinţa plantelor de a le acumula, ceea ce poate duce la creşterea concentraţiei de cadmiu şi plumb în frunzele de salată verde. The disadvantage of this process is that the sodium gumate solution is introduced into the soil by irrigation in relatively large quantities (300 L/ha); the process reduces the phytotoxicity of heavy metals, but does not reduce the tendency of plants to accumulate them, which can lead to an increase in the concentration of cadmium and lead in lettuce leaves.
Problema pe care o rezolvă invenţia constă în reducerea tendinţei plantelor de salată verde de a acumula metalele grele - cadmiu şi plumb, în frunze prin tratarea seminţelor înainte de semănat cu substanţe biologic active de origine vegetală. The problem solved by the invention consists in reducing the tendency of lettuce plants to accumulate heavy metals - cadmium and lead, in the leaves by treating the seeds before sowing with biologically active substances of plant origin.
Procedeul de tratare a seminţelor de salată verde constă în înmuierea seminţelor de salată verde Lactuca sativa L. înainte de semănat timp de 2 ore într-o soluţie apoasă de 0,0005… 0,1% de glicozide feniletanoidice, obţinute din plante Veronica chamaedrys L. prin extragere la fierbere cu alcool butilic saturat cu apă, concentrare, purificare şi uscare în vid a extractului obţinut. The lettuce seed treatment process consists of soaking lettuce seeds Lactuca sativa L. before sowing for 2 hours in an aqueous solution of 0.0005… 0.1% of phenylethanoid glycosides, obtained from Veronica chamaedrys L. plants by boiling extraction with butyl alcohol saturated with water, concentration, purification and vacuum drying of the obtained extract.
Rezultatul constă în reducerea acumulării metalelor grele - cadmiu şi plumb, în frunzele plantelor de salată verde. The result is a reduction in the accumulation of heavy metals - cadmium and lead - in the leaves of lettuce plants.
Procedeul contribuie la îmbunătăţirea calităţii produsului alimentar, permite lărgirea sortimentului de salată verde care poate fi cultivată şi în zonele agricole cu risc înalt de poluare. The process contributes to improving the quality of the food product, allowing for a wider range of lettuce that can be grown in agricultural areas with a high risk of pollution.
Extractul integral de glicozide feniletanoidice (GFE) din Veronica chamaedrys L. a fost obţinut prin extragerea de trei ori a părţilor aeriene uscate de plante prin fierbere în alcool butilic saturat cu apă, concentrarea extractelor prin evaporare, purificarea reziduului cu cloroform, precipitarea glicozidelor feniletanoidice din acetonă şi uscarea în vid la 40°C. Conform datelor analizei cromatografice şi spectrale, în extractul integral de suma glicozidelor feniletanoidice au fost identificate următoarele componente majore: acteozidă, ehrenozidă şi chamaedrozidă [Marchenko A., Kintia P,. Mashcenco N. et al. Phenylethanoid and iridoid glycosides from Veronica chamaedrys L. Chemistry Journal of Moldova. General, Industrial and Ecological Chemistry, 2008, 3(2), p. 101-104]. The integral extract of phenylethanoid glycosides (GFE) from Veronica chamaedrys L. was obtained by three times extraction of the dried aerial parts of the plants by boiling in butyl alcohol saturated with water, concentration of the extracts by evaporation, purification of the residue with chloroform, precipitation of phenylethanoid glycosides from acetone and drying in vacuum at 40°C. According to the data of chromatographic and spectral analysis, the following major components were identified in the integral extract of the sum of phenylethanoid glycosides: acteoside, ehrenoside and chamaedroside [Marchenko A., Kintia P,. Mashcenco N. et al. Phenylethanoid and iridoid glycosides from Veronica chamaedrys L. Chemistry Journal of Moldova. General, Industrial and Ecological Chemistry, 2008, 3(2), p. 101-104].
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările au fost efectuate în condiţii de câmp pe fond poluat natural, situat la 100 m distanţă de autostradă. Obiectul de studiu a fost salata verde Lactuca sativa L. de soiurile Novogodnii - cu tendinţă înaltă de acumulare a metalelor grele, şi Moskovskii parnikovîi N3 - cu tendinţă medie de acumulare a metalelor grele. The research was conducted in field conditions on a naturally polluted background, located 100 m away from the highway. The object of study was lettuce Lactuca sativa L. of the Novogodnii varieties - with a high tendency to accumulate heavy metals, and Moskovskii parnikovii N3 - with an average tendency to accumulate heavy metals.
Înainte de semănat, o parte din seminţe au fost înmuiate timp de 2 ore în soluţii apoase de GFE în concentraţiile de 0,0005; 0,005; 0,01; 0,1% (invenţia propusă); altă parte au fost înmuiate în soluţie apoasă de gumat de sodiu cu concentraţia de 0,0175% ( cea mai apropiată soluţie). În calitate de martor au servit seminţele înmuiate în apă. Umectarea seminţelor s-a efectuat conform condiţiilor stabilite [Preparate chimice şi biologice de protecţie şi stimularea creşterii plantelor. Chişinău, Ştiinţa, 1997, ISBN 9975.67-420-8, p. 90]. După înmuiere seminţele au fost uscate timp de o oră, apoi semănate manual în câmp în prima decadă a lunii iunie, pe parcele cu suprafaţa de 1 m2, în patru repetări pentru fiecare variantă conform metodei Dospehov [Доспехов Б.А. Методика полевого опыта. Москва, Агропромиздат, 1985, р. 351]. Before sowing, part of the seeds were soaked for 2 hours in aqueous solutions of GFE in concentrations of 0.0005; 0.005; 0.01; 0.1% (the proposed invention); another part was soaked in an aqueous solution of sodium gumate with a concentration of 0.0175% (the closest solution). Seeds soaked in water served as a control. Wetting of the seeds was carried out according to the established conditions [Chemical and biological preparations for protection and stimulation of plant growth. Chisinau, Ştiinta, 1997, ISBN 9975.67-420-8, p. 90]. After soaking, the seeds were dried for one hour, then sown manually in the field in the first decade of June, on plots with an area of 1 m2, in four repetitions for each variant according to the Dospehov method [Доспехов B.А. Методика полевого опыта. Москва, Агропромиздат, 1985, p. 351].
Plantele au fost cultivate conform tehnologiei convenţionale. Conţinutul de metale grele a fost determinat în stadiul de maturitate tehnică a frunzelor în perioada recoltării conform metodei spectroscopiei atomice de absorbţie [ГОСТ 26929-94. Сырье и продукты пищевые. Подготовка проб. Минерализация для определения токсичных элементов] în laboratorul acreditat pentru testarea produselor alimentare, materiei prime, solurilor, apelor şi fertilizanţior din Federaţia Rusă OOO ЦСЭМ «Московский», reieşind din calculul metalelor grele mg/kg de frunze proaspete şi concentraţia limită admisă de cadmiu 0,030 mg/kg, iar concentraţia plumbului de 0,500 mg/kg de frunze proaspete. The plants were grown according to conventional technology. The content of heavy metals was determined at the stage of technical maturity of the leaves during the harvest period according to the atomic absorption spectroscopy method [GOST 26929-94. Сырье и продукты пишевые. Подготовка проб. Миналеризация для определительных элементов] in the accredited laboratory for testing food products, raw materials, soils, waters and fertilizers in the Russian Federation OOO ЦСЭМ «Московский», based on the calculation of heavy metals mg/kg of fresh leaves and the permissible limit concentration of cadmium 0.030 mg/kg, and the concentration of lead 0.500 mg/kg of fresh leaves.
Rezultatele obţinute denotă că în varianta martor (apă) conţinutul de cadmiu în frunzele de salată de soiurile Novogodnii (0,038 mg/kg) şi Moskovskii parnikovîi N3 (0,034 mg/kg ) în stadiul de maturitate tehnică depăşeşte concentraţia limită admisă. În cea mai apropiată soluţie concentraţia cadmiului depăşeşte şi mai mult limita admisă 0,045 şi 0,037 mg/kg respectiv. În cazul tratării seminţelor înainte de semănat cu soluţie de 0,0005% de GFE conţinutul cadmiului în frunzele de salată de soiurile Novogodnii şi Moskovskii parnikovîi N3 scade sub nivelul concentraţiei limită admise cu 34,2% şi 17,6%, respectiv, în raport cu varianta martor şi cu 80,1% şi, respectiv, 32,1% în raport cu varianta proximă. Concentraţia optimală a GFE este de 0,0005% (tab. 1). The results obtained show that in the control variant (water) the cadmium content in lettuce leaves of the Novogodnii (0.038 mg/kg) and Moskovskii parnikovii N3 (0.034 mg/kg) varieties at the stage of technical maturity exceeds the permissible limit concentration. In the closest solution the cadmium concentration exceeds the permissible limit even more 0.045 and 0.037 mg/kg respectively. In the case of seed treatment before sowing with a 0.0005% solution of GFE the cadmium content in lettuce leaves of the Novogodnii and Moskovskii parnikovii N3 varieties decreases below the permissible limit concentration by 34.2% and 17.6%, respectively, in relation to the control variant and by 80.1% and, respectively, 32.1% in relation to the closest variant. The optimal concentration of GFE is 0.0005% (table 1).
Tabelul 1 Table 1
Influenţa GFE asupra procesului de acumulare a cadmiului în frunzele de salată verde The influence of GFE on the process of cadmium accumulation in lettuce leaves
Soiul de salată Varianta/concentraţia, % Conţinutul de cadmiu mg/kg % faţă de martor % faţă de cea mai apropriată soluţie Novogodnii Martor (H2O) 0,038 Gumat de sodiu 0,0175% 0,045 18,4 GFE 0,1% 0,029 23,7 55,2 GFE 0,01% 0,028 26,3 60,7 GFE 0,005 0,026 31,6 73,1 GFE 0,0005% 0,025 34,2 80,1 НСР05 0,007 Moscovskii parnicovîi N3 Martor (H2O) 0,034 Gumat de sodiu 0,0175 % 0,037 8,8 GFE 0,1% 0,031 8,8 19,4 GFE 0,01% 0,029 14,7 27,6 GFE 0,005% 0,029 14,7 27,6 GFE 0,0005% 0,028 17,6 32,1 НСР05 0,004 Concentraţia limită admisă 0,030 Lettuce variety Variant/concentration, % Cadmium content mg/kg % compared to control % compared to the most appropriate solution Novogodnii Control (H2O) 0.038 Sodium humate 0.0175% 0.045 18.4 GFE 0.1% 0.029 23.7 55.2 GFE 0.01% 0.028 26.3 60.7 GFE 0.005 0.026 31.6 73.1 GFE 0.0005% 0.025 34.2 80.1 НСР05 0.007 Moscovskii parnikovoi N3 Control (H2O) 0.034 Sodium humate 0.0175% 0.037 8.8 GFE 0.1% 0.031 8.8 19.4 GFE 0.01% 0.029 14.7 27.6 GFE 0.005% 0.029 14.7 27.6 GFE 0.0005% 0.028 17.6 32.1 НСР05 0.004 Admissible limit concentration 0.030
În tab. 2 se observă acelaşi efect al GFE de sporire a rezistenţei plantelor de salată verde faţă de acumularea plumbului în frunze. În cazul soiului de salată Novogodnii cu tendinţă înaltă de acumulare a metalelor grele concentraţia plumbului în varianta cu GFE 5х 10-4 % este cu 29,5% mai mică în raport cu varianta martor şi cu 50% în raport cu varianta proximă. In tab. 2, the same effect of GFE on increasing the resistance of lettuce plants to lead accumulation in leaves is observed. In the case of the Novogodnii lettuce variety with a high tendency to accumulate heavy metals, the lead concentration in the variant with GFE 5х 10-4 % is 29.5% lower compared to the control variant and 50% lower compared to the close variant.
Tabelul 2 Table 2
Influenţa GFE asupra procesului de acumulare a plumbului în frunzele de salată verde The influence of GFE on the process of lead accumulation in lettuce leaves
Soiul de salată Varianta/concentraţia, % Conţinutul de plumb mg/kg % faţă de martor % faţă de cea mai apropriată soluţie Novogodnii Martor (H2O) 0,088 Gumat de sodiu 0,0175 % 0,093 5,7 GFE 0,1 % 0,071 19,3 31,0 GFE 0,01% 0,068 22,7 36,8 GFE 0,005% 0,066 25,0 40,9 GFE 0,0005% 0,062 29,5 50,0 НСР05 0,022 Moscovskii parnicovîi N3 Martor (H2O) 0,080 Gumat de sodiu 0,0175 % 0,082 2,5 GFE 0,1 % 0,077 3,7 6,5 GFE 0,01% 0,075 6,2 9,3 GFE 0,005% 0,076 5,0 7,9 GFE 0,0005% 0,075 6,2 9,3 НСР05 0,006 Concentraţia limită admisă 0,500 Lettuce variety Variant/concentration, % Lead content mg/kg % compared to control % compared to the most appropriate solution Novogodnii Control (H2O) 0.088 Sodium humate 0.0175 % 0.093 5.7 GFE 0.1 % 0.071 19.3 31.0 GFE 0.01% 0.068 22.7 36.8 GFE 0.005% 0.066 25.0 40.9 GFE 0.0005% 0.062 29.5 50.0 НСР05 0.022 Moscovskii parnikovoi N3 Control (H2O) 0.080 Sodium humate 0.0175 % 0.082 2.5 GFE 0.1 % 0.077 3.7 6.5 GFE 0.01% 0.075 6.2 9.3 GFE 0.005% 0.076 5.0 7.9 GFE 0.0005% 0.075 6.2 9.3 НСР05 0.006 Admissible limit concentration 0.500
Conform rezultatelor obţinute, concentraţia optimală a soluţiei de GFE este de 0,0005%. According to the results obtained, the optimal concentration of the GFE solution is 0.0005%.
Prin urmare, tratarea seminţelor de salată verde înainte de semănat cu soluţii de GFE este un procedeu agrotehnic simplu, ecologic inofensiv, care micşorează concentraţia de metale grele - cadmiu şi plumb, în frunze prin reducerea tendinţei plantelor de a le acumula, contribuie la sporirea calităţii produsului vegetal de consum curent şi permite lărgirea sortimentului de salată verde ce poate fi cultivată şi în zonele agricole cu risc înalt de poluare. Therefore, treating lettuce seeds before sowing with GFE solutions is a simple, ecologically harmless agrotechnical process that reduces the concentration of heavy metals - cadmium and lead - in leaves by reducing the tendency of plants to accumulate them, contributes to increasing the quality of the vegetable product for current consumption and allows the expansion of the lettuce assortment that can be cultivated in agricultural areas with a high risk of pollution.
1. Ульяненко Л.Н., Круглов С.В., Филипас А.С. и др. Влияние гумата натрия на развитие растений салата и накопление тяжелых металлов в урожае. Агрохимия, 2004, № 4, p. 58-64 1. Ulyanenko L.N., Kruglov S.V., Filipas A.S. and others The influence of sodium humate on the development of salad plants and the accumulation of heavy metals in crops. Agrochemistry, 2004, № 4, p. 58-64
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Citations (4)
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|---|---|---|---|---|
| RU2011028C1 (en) * | 1991-01-09 | 1994-04-15 | Сизов Геннадий Михайлович | Hydraulic pressure relay |
| EP1036492A1 (en) * | 1999-03-13 | 2000-09-20 | Aventis Research & Technologies GmbH & Co. KG | Seed treatment composition |
| MD2037F2 (en) * | 2001-04-26 | 2002-11-30 | Inst Genetica Rep Moldova | Process for obtaining the 22-O-hydroxyfurostanols |
| MD3865C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 3,4-dihydroxy-b-phenylethoxy-O-a-L-rhamnopyranosyl-(1→3)-4-O-cafeoyl-a-L-arabinopyranoside as compound increasing the seed productivity |
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| EP1036492A1 (en) * | 1999-03-13 | 2000-09-20 | Aventis Research & Technologies GmbH & Co. KG | Seed treatment composition |
| MD2037F2 (en) * | 2001-04-26 | 2002-11-30 | Inst Genetica Rep Moldova | Process for obtaining the 22-O-hydroxyfurostanols |
| MD3865C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 3,4-dihydroxy-b-phenylethoxy-O-a-L-rhamnopyranosyl-(1→3)-4-O-cafeoyl-a-L-arabinopyranoside as compound increasing the seed productivity |
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| Publication number | Publication date |
|---|---|
| MD957Y (en) | 2015-10-31 |
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