MD593G2 - Method for cianocobalamine determination - Google Patents
Method for cianocobalamine determinationInfo
- Publication number
- MD593G2 MD593G2 MD94-0302A MD940302A MD593G2 MD 593 G2 MD593 G2 MD 593G2 MD 940302 A MD940302 A MD 940302A MD 593 G2 MD593 G2 MD 593G2
- Authority
- MD
- Moldova
- Prior art keywords
- cianocobalamine
- determination
- optical density
- wave length
- extraction
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000003287 optical effect Effects 0.000 abstract 3
- 239000002028 Biomass Substances 0.000 abstract 2
- 238000000605 extraction Methods 0.000 abstract 2
- 239000007791 liquid phase Substances 0.000 abstract 2
- 230000006641 stabilisation Effects 0.000 abstract 2
- 238000011105 stabilization Methods 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000007900 aqueous suspension Substances 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000002798 spectrophotometry method Methods 0.000 abstract 1
Landscapes
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to the micribiology, particularly to the method for cianocobalamine determination into the propyonic acid bacteria biomass including the cianocobalamine extraction from the biomass aqueous suspension at heating in presence of KCN, separation of liquid phase by centrifugation, stabilization thereof with the subsequent determination of cianocobalamine by means of spectrophotometry, characterised by the fact that the cianocobalamine extraction is realized at pH=6-7, the liquid phase stabilization after centirifugation is provided at pH=8,5-10 in the dark during 1 hour, and the cianocobalamine amount is determined by calculation according to the formula:, whereinC - is the cianocobalamine content, %D540 - the optical density at the wave length of 540 nm,D498 - the optical density at the wave length of 498 nm,D582 - the optical density at the wave length of 582 nm,L - is the cuvette width.The technical result of the method consists in the simplification and decreasing of the cianocobalamine amount determination time, and increasing at the expense of this the pression and reproductibility of the method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD94-0302A MD593G2 (en) | 1994-09-21 | 1994-09-21 | Method for cianocobalamine determination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD94-0302A MD593G2 (en) | 1994-09-21 | 1994-09-21 | Method for cianocobalamine determination |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD940302A MD940302A (en) | 1996-03-29 |
| MD593F2 MD593F2 (en) | 1996-07-31 |
| MD593G2 true MD593G2 (en) | 1997-03-31 |
Family
ID=19738577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD94-0302A MD593G2 (en) | 1994-09-21 | 1994-09-21 | Method for cianocobalamine determination |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD593G2 (en) |
-
1994
- 1994-09-21 MD MD94-0302A patent/MD593G2/en active IP Right Grant
Non-Patent Citations (3)
| Title |
|---|
| Березовский В.М. Химия витаминов, 2-е изд., M, Пищевая промышленность, 1973, c. 578. * |
| Пронякова Г.В. ДАН СССР, 1958, т. 123, № 2, c. 331. * |
| Пушкина Н.Н. Биохимические методы исследований, Медгиз. 1963, c. 54. * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD593F2 (en) | 1996-07-31 |
| MD940302A (en) | 1996-03-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20140921 |