MD4818C8 - Composition for cultivated plants - Google Patents
Composition for cultivated plants Download PDFInfo
- Publication number
- MD4818C8 MD4818C8 MDA20210024A MD20210024A MD4818C8 MD 4818 C8 MD4818 C8 MD 4818C8 MD A20210024 A MDA20210024 A MD A20210024A MD 20210024 A MD20210024 A MD 20210024A MD 4818 C8 MD4818 C8 MD 4818C8
- Authority
- MD
- Moldova
- Prior art keywords
- plants
- activity
- corn
- conditions
- thiourea
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 16
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 claims abstract description 28
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 14
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims abstract description 9
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000011591 potassium Substances 0.000 claims abstract description 9
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 9
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical compound [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000011777 magnesium Substances 0.000 claims abstract description 4
- 229910052749 magnesium Inorganic materials 0.000 claims abstract description 4
- 244000038559 crop plants Species 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 30
- 244000068988 Glycine max Species 0.000 abstract description 25
- 240000008042 Zea mays Species 0.000 abstract description 23
- 230000003078 antioxidant effect Effects 0.000 abstract description 22
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 20
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 20
- 235000005822 corn Nutrition 0.000 abstract description 20
- 239000003642 reactive oxygen metabolite Substances 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 3
- 239000013543 active substance Substances 0.000 abstract description 2
- 230000001413 cellular effect Effects 0.000 abstract description 2
- 238000010525 oxidative degradation reaction Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 42
- 239000000243 solution Substances 0.000 description 18
- 108090000790 Enzymes Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 14
- 239000003963 antioxidant agent Substances 0.000 description 13
- 235000010469 Glycine max Nutrition 0.000 description 9
- 235000006708 antioxidants Nutrition 0.000 description 9
- 230000036542 oxidative stress Effects 0.000 description 7
- 102100037373 DNA-(apurinic or apyrimidinic site) endonuclease Human genes 0.000 description 6
- 102100033944 Glycine receptor subunit alpha-2 Human genes 0.000 description 6
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 6
- 230000009467 reduction Effects 0.000 description 4
- 235000007244 Zea mays Nutrition 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 235000012054 meals Nutrition 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical class OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 108060006004 Ascorbate peroxidase Proteins 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 108010063907 Glutathione Reductase Proteins 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 102100036442 Glutathione reductase, mitochondrial Human genes 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
Invenţia se referă la agricultură, şi anume la compoziţii chimice cu proprietăţi de substanţe biologic active, care pot fi utilizate pentru reducerea impactului negativ al speciilor reactive de oxigen asupra plantelor de porumb şi soia şi pentru diminuarea destrucţiei oxidative a componentelor celulare. The invention relates to agriculture, namely to chemical compositions with properties of biologically active substances, which can be used to reduce the negative impact of reactive oxygen species on corn and soybean plants and to reduce the oxidative destruction of cellular components.
Este cunoscută utilizarea tioureei pentru protecţia plantelor, care posedă proprietăţi de diminuare a formării dialdehidei malonice şi majorare a activităţii sistemelor de protecţie antioxidantă a plantelor atât în condiţii favorabile, cât şi nefavorabile din mediul ambiant [1]. Dezavantajul soluţiei cunoscute constă în proprietăţile antioxidante insuficient exprimate ale tioureei în diverse condiţii de mediu. The use of thiourea for plant protection is known, which possesses properties of reducing the formation of malonic dialdehyde and increasing the activity of the antioxidant protection systems of plants both in favorable and unfavorable environmental conditions [1]. The disadvantage of the known solution consists in insufficiently expressed antioxidant properties of thiourea in various environmental conditions.
Problema pe care o rezolvă invenţia propusă este elaborarea unei compoziţii pe bază de tiouree, utilizarea căreia să asigure proprietăţi antioxidante sporite pentru protecţia plantelor de la destrucţiile structurilor celulare cauzate de speciile reactive de oxigen în diverse condiţii de mediu. The problem that the proposed invention solves is the development of a composition based on thiourea, the use of which ensures increased antioxidant properties for the protection of plants from the destruction of cellular structures caused by reactive oxygen species in various environmental conditions.
Problema se rezolvă prin compoziţia pentru plantele de cultură, denumită convenţional Tiogalmet, ce conţine 66,7% de tiouree şi 33,3% de amestec de galaţi de potasiu, amoniu, magneziu, molibdat de potasiu şi paramolibdat de amoniu, luaţi în raport masic de 1:1:1:0,1:0,1, respectiv. The problem is solved by the composition for cultivated plants, conventionally called Thiogalmet, which contains 66.7% of thiourea and 33.3% of a mixture of potassium, ammonium, magnesium gallates, potassium molybdate and ammonium paramolybdate, taken in mass ratio of 1:1:1:0,1:0,1, respectively.
Amestecul de galaţi (săruri ale acidului 3,4,5-trihidroxibenzoic) de K+, NH4 +, Mg2+ şi săruri de molibdat de potasiu şi paramolibdat de amoniu este cunoscut sub denumirea de Galmet şi posedă proprietăţi de stimulare a creşterii plantelor (Şefîrţă A., Brînză L., Toma S., Bulhac I. şi al. Opţiuni fiziologice de fortificare a plantelor în condiţii de insuficienţă de umiditate. În: Diminuarea impactului factorilor pedoclimatici extremali asupra plantelor de cultură. Chişinău, 2008, p. 171-191). The mixture of gallates (salts of 3,4,5-trihydroxybenzoic acid) of K+, NH4 +, Mg2+ and salts of potassium molybdate and ammonium paramolybdate is known as Galmet and possesses plant growth stimulating properties (Sefîrtă A. , Brînza L., Toma S., Bulhac I. et al. Physiological options for strengthening plants in conditions of insufficient humidity. In: Reducing the impact of extreme pedoclimatic factors on crop plants. Chisinau, 2008, p. 171-191) .
Rezultatul tehnic al invenţiei constă în asigurarea majorării proprietăţilor antioxidante ale plantelor de porumb şi soia în condiţii optime de umiditate respectiv cu circa 27% şi 17% comparativ cu martorul şi cu 43% şi 19% în condiţii de insuficienţă de umiditate. Rezultatul tehnic constă în intensificarea activităţii enzimelor de protecţie antioxidantă, exprimată prin diminuarea conţinutului dialdehidei malonice - produsul final al oxidării peroxidice a lipidelor de către speciile reactive de oxigen. The technical result of the invention consists in ensuring the increase of the antioxidant properties of corn and soybean plants under optimal humidity conditions, respectively by about 27% and 17% compared to the control and by 43% and 19% in insufficient humidity conditions. The technical result consists in the intensification of the activity of antioxidant protection enzymes, expressed by the decrease in the content of malonic dialdehyde - the final product of the peroxidic oxidation of lipids by reactive oxygen species.
Avantajele invenţiei constau în efectul mai mare asupra gradului de majorare a proprietăţilor antioxidante ale plantelor comparativ cu cea mai apropiată soluţie tehnică: cu 17% şi 7,0 % respectiv la porumb şi soia în condiţii optime şi cu 20,6% şi 6,0% în condiţii de secetă moderată. The advantages of the invention consist in the greater effect on the degree of increase in the antioxidant properties of plants compared to the closest technical solution: by 17% and 7.0% respectively for corn and soybeans under optimal conditions and by 20.6% and 6.0 % in moderate drought conditions.
Exemple de realizare a invenţiei Examples of realization of the invention
Exemplul I. Caracteristica compoziţiei Example I. Characteristic of the composition
Tiogalmetul reprezintă o substanţă solidă în formă de pulbere de culoare cafeniu-deschis, stabilă la temperatura camerei, bine solubilă în apă. S-a obţinut prin amestecarea a două părţi de masă de tiouree cu o parte de masă de Galmet, care, la rândul său, reprezintă un preparat ce constă din galaţi de potasiu, amoniu, magneziu, molibdat de potasiu şi paramolibdat de amoniu luaţi într-un raport de masă respectiv de 1:1:1:0,1:0,1. Thiogalmet is a solid substance in the form of light-brown powder, stable at room temperature, well soluble in water. It was obtained by mixing two parts by mass of thiourea with one part by mass of Galmet, which, in turn, is a preparation consisting of gallates of potassium, ammonium, magnesium, potassium molybdate and ammonium paramolybdate taken in a respective mass ratio of 1:1:1:0.1:0.1.
Spectrul IR al compoziţiei Tiogalmet se caracterizează prin prezenţa mai multor benzi de absorbţie de diferită intensitate, cunoaşterea cărora va permite folosirea spectrului în calitate de reper la obţinerea repetată a compoziţiei respective. IR (í, cm-1): 3623sl, 3361m, 3251i, 3163i, 3083m, 2989i, 2686m, 2647sl, 1680sl, 1607m, 1539f.i, 1456i, 1399f.i, 1366f.i, 1345f.i, 1317um, 1276i, 1262i, 1220sl, 1201i, 1096m, 1082m, 1055i, 1044i, 965sl, 890um, 876m, 836sl, 800m, 792m, 773m, 748m, 729i, 672m, 631i, 488f.i, 467i, 412sl (f.i - foarte intensivă, i - intensivă, m - medie, sl - slabă, f.sl - foarte slabă, um - umăr) The IR spectrum of the Tiogalmet composition is characterized by the presence of several absorption bands of different intensity, the knowledge of which will allow the use of the spectrum as a benchmark for the repeated preparation of the respective composition. IR (í, cm-1): 3623sl, 3361m, 3251i, 3163i, 3083m, 2989i, 2686m, 2647sl, 1680sl, 1607m, 1539f.i, 1456i, 1399f.i, 1366f.i, 1345f.i, 13 17um, 1276i , 1262i, 1220sl, 1201i, 1096m, 1082m, 1055i, 1044i, 965sl, 890um, 876m, 836sl, 800m, 792m, 773m, 748m, 729i, 672m, 631i, 488f.i , 467i, 412sl (f.i - very intensive, i - intensive, m - medium, sl - weak, f.sl - very weak, um - shoulder)
Exemplul II. Demonstrarea proprietăţilor antioxidante şi argumentarea efectului major comparativ cu cea mai apropiată soluţie tehnică. Example II. Demonstrating the antioxidant properties and arguing the major effect compared to the closest technical solution.
În experienţe de vegetaţie s-a studiat efectul tratării plantelor conform celei mai apropiate soluţii tehnice şi cu soluţie de Tiogalmet, asupra indicilor care caracterizează intensitatea destrucţiilor oxidative (conţinutul dialdehidei malonice - DAM) şi activităţii enzimelor de protecţie antioxidantă (superoxiddismutazei - SOD, catalazei - CAT, ascorbatperoxidazei - APX, glutationproxidazei - GLPX, glutationreductazei - GLR), în frunzele plantelor de porumb şi soia, crescute în condiţii optime. Experienţele s-au realizat conform schemei: I-a variantă - plante din seminţe tratate cu apă (martor); a II-a variantă - plante din seminţe tratate cu soluţie apoasă de tiouree în concentraţie de 0,005 % (cea mai apropiată soluţie tehnică); a III-a variantă - plante din seminţe tratate cu soluţie apoasă de Tiogalmet în concentraţie de 0,005%. S-a studiat efectul pretratării plantelor asupra particularităţilor de protecţie antioxidantă a plantelor în condiţii de umiditate optimă şi de stres oxidativ, creat prin reducerea umidităţii solului. Rezultatele sunt prezentate în tabelele 1-3. In vegetation experiments, the effect of treating plants according to the closest technical solution and with Thiogalmet solution was studied, on the indices that characterize the intensity of oxidative destruction (malonic dialdehyde content - DAM) and the activity of antioxidant protection enzymes (superoxide dismutase - SOD, catalase - CAT, ascorbate peroxidase - APX, glutathione peroxidase - GLPX, glutathione reductase - GLR), in the leaves of corn and soybean plants, grown under optimal conditions. The experiments were carried out according to the scheme: 1st variant - plants from seeds treated with water (control); 2nd variant - plants from seeds treated with an aqueous solution of thiourea in a concentration of 0.005% (the closest technical solution); 3rd variant - plants from seeds treated with an aqueous solution of Thiogalmet in a concentration of 0.005%. The effect of pretreatment of plants on the antioxidant protection characteristics of plants under conditions of optimal humidity and oxidative stress, created by reducing soil moisture, was studied. The results are presented in Tables 1-3.
Tabelul 1 Table 1
Conţinutul dialdehidei malonice şi activitatea enzimelor de protecţie antioxidantă în frunzele plantelor de porumb şi soia, crescute în condiţii de umiditate optimă The content of malonic dialdehyde and the activity of antioxidant protection enzymes in the leaves of corn and soybean plants, grown under optimal humidity conditions
Variante Indici Martor Tiouree Tiogalmet M ± m M ± m δ, %M* M ± m δ,%M* Zea mays, L. cv. P458 Conţinutul DAM, µM · g-1 m.p. 33,66±0,24 31,44±0,09 -6,59 24,91±0,04 -26,00 Activitatea SOD, un. conv. · g-1 m.p. 17,44±1,17 19,87±0,83 13,93 22,08±0,57 26,60 Activitatea CAT, mM · g-1 m.p. 7,98±0,02 8,60±0,11 7,78 10,73±0,47 34,46 Activitatea APX, mM · g-1 m.p. 4,24±0,19 5,51±0,46 29,95 5,61±0,26 32,31 Activitatea GLPX, mM · g-1 m.p. 46,36±0,47 52,83±0,25 13,95 62,45±4,98 34,70 Activitatea GLR, mM · g-1 m.p. 69,41±6,57 82,16±4,32 18,37 85,00±3,67 22,46 Glycine max (Merr), L., cv. Nadejda Conţinutul DAM, µM· g-1 m.p. 32,87±0,04 25,38±0,06 -22,78 24,67±0,70 -24,95 Activitatea SOD, un. conv. · g-1 m.p. 26,28±1,59 28,16±1,86 7,15 30,30±1,73 15,29 Activitatea CAT, mM · g-1 m.p. 6,04±0,22 6,49±0,05 7,45 6,65±0,25 10,09 Activitatea APX, mM · g-1 m.p. 5,26±0,24 6,02±0,09 14,44 6,51±0,27 23,76 Activitatea GLPX, mM · g-1 m.p. 59,56±0,61 63,75±2,06 7,03 68,07±1,33 14,28 Activitatea GLR, mM · g-1 m.p. 106,61±3,79 117,46±7,03 10,18 126,59±3,2 18,74 Variants Indices Control Tiouree Thiogalmet M ± m M ± m δ, %M* M ± m δ,%M* Zea mays, L. cv. P458 DAM content, µM · g-1 m.p. 33.66±0.24 31.44±0.09 -6.59 24.91±0.04 -26.00 SOD activity, un. conv. · g-1 m.p. 17.44±1.17 19.87±0.83 13.93 22.08±0.57 26.60 CAT activity, mM · g-1 m.p. 7.98±0.02 8.60±0.11 7.78 10.73±0.47 34.46 APX activity, mM · g-1 m.p. 4.24±0.19 5.51±0.46 29.95 5.61±0.26 32.31 GLPX activity, mM · g-1 m.p. 46.36±0.47 52.83±0.25 13.95 62.45±4.98 34.70 GLR activity, mM · g-1 m.p. 69.41±6.57 82.16±4.32 18.37 85.00±3.67 22.46 Glycine max (Merr), L., cv. Nadejda DAM content, µM· g-1 m.p. 32.87±0.04 25.38±0.06 -22.78 24.67±0.70 -24.95 SOD activity, un. conv. · g-1 m.p. 26.28±1.59 28.16±1.86 7.15 30.30±1.73 15.29 CAT activity, mM · g-1 m.p. 6.04±0.22 6.49±0.05 7.45 6.65±0.25 10.09 APX activity, mM · g-1 m.p. 5.26±0.24 6.02±0.09 14.44 6.51±0.27 23.76 GLPX activity, mM · g-1 m.p. 59.56±0.61 63.75±2.06 7.03 68.07±1.33 14.28 GLR activity, mM · g-1 m.p. 106.61±3.79 117.46±7.03 10.18 126.59±3.2 18.74
*- comparativ cu varianta martor; m.p. - masă proaspătă *- compared to the control version; m.p. - fresh meal
În condiţii satisfăcătoare de umiditate în sol plantele martor de porumb şi soia se caracterizează prin conţinut de dialdehidă malonică aproximativ egal, dar care indică existenţa unui stres oxidativ moderat, generat de temperatura ridicată şi umiditatea relativă a aerului scăzută din timpul vegetaţiei. Pretratarea plantelor cu remediile respective a condiţionat o diminuare a conţinutului DAM la reprezentanţii ambelor culturi: la plantele de porumb şi soia, pretratate cu tiouree s-a înregistrat o scădere cu 6,59 la sută, la soia - cu 22,79%. La plantele de porumb şi soia pretratate cu soluţie de Tiogalmet gradul de diminuare a conţinutului DAM a constituit respectiv 26,00% şi 24,95% comparativ cu conţinutul DAM în frunzele plantelor martor. Utilizarea noului produs în condiţii normale de umiditate a asigurat o reducere a stresului oxidativ la plantele de porumb şi soia cu 20,77% şi 2,79% comparativ cu gradul de diminuare asigurat de pretratarea conform celei mai apropriate soluţii tehnice. Efectul major de diminuare a stresului oxidativ (conţinutului DAM) la plantele pretratate cu soluţie de Tiogalmet se datorează intensificării mai semnificative a activităţii enzimelor de protecţie antioxidantă. Astfel noua combinaţie a asigurat la plantele de porumb o majorare a activităţii SOD, CAT, APX, GLPX şi GLR cu, %: 11,12; 24,77; 1,81; 18,21 şi 3,45 comparativ cu soluţia tehnică cunoscută. La plantele de soia gradul de majorare a activităţii acestor enzime a constituit, respectiv, %: 7,60; 2,46; 8,13; 6,77 şi 7,77. In satisfactory soil moisture conditions, the control plants of corn and soybeans are characterized by an approximately equal content of malonic dialdehyde, but which indicates the existence of a moderate oxidative stress, generated by the high temperature and the low relative humidity of the air during the vegetation. The pretreatment of the plants with the respective remedies caused a decrease in the DAM content in the representatives of both cultures: in the corn and soybean plants, pretreated with thiourea, there was a decrease of 6.59 percent, in soybeans - by 22.79%. In corn and soybean plants pretreated with Tiogalmet solution, the degree of reduction in DAM content was respectively 26.00% and 24.95% compared to the DAM content in the leaves of control plants. The use of the new product under normal humidity conditions ensured a reduction of oxidative stress in corn and soybean plants by 20.77% and 2.79% compared to the degree of reduction ensured by the pretreatment according to the most appropriate technical solution. The major effect of reducing oxidative stress (DAM content) in plants pretreated with Thiogalmet solution is due to the more significant intensification of the activity of antioxidant protection enzymes. Thus, the new combination ensured in corn plants an increase in the activity of SOD, CAT, APX, GLPX and GLR by %: 11.12; 24.77; 1.81; 18.21 and 3.45 compared to the known technical solution. In soybean plants, the degree of increase in the activity of these enzymes was, respectively, %: 7.60; 2.46; 8.13; 6.77 and 7.77.
Deci, compoziţia propusă asigură obţinerea unor proprietăţi antioxidante şi utilizarea ei pentru pretratarea plantelor în condiţii optime de umiditate asigură o ameliorare a status-ului redox prin activarea enzimelor antioxidante şi diminuarea conţinutului de DAM. So, the proposed composition ensures obtaining some antioxidant properties and its use for the pretreatment of plants in optimal humidity conditions ensures an improvement of the redox status by activating antioxidant enzymes and reducing the content of DAM.
Efect benefic al pretratării plantelor cu soluţie de Tiogalmet a fost înregistrat şi la determinarea proprietăţilor antioxidante ale plantelor în condiţii de stres oxidativ cauzat de insuficienţa moderată de umiditate (tabelul 2). Beneficial effect of the pretreatment of plants with Thiogalmet solution was also recorded when determining the antioxidant properties of plants under conditions of oxidative stress caused by moderate moisture deficiency (table 2).
Tabelul 2 Table 2
Conţinutul dialdehidei malonice şi activitatea enzimelor de protecţie antioxidantă în frunzele plantelor de porumb şi soia crescute în condiţii de secetă moderată The content of malonic dialdehyde and the activity of antioxidant protection enzymes in the leaves of corn and soybean plants grown under moderate drought conditions
Variante Indici Martor Tiouree Tiogalmet M ± m M ± m δ, %M* M ± m δ, %M* Zea mays, L. cv. P458 Conţinutul DAM, µM · g-1 m.p. 47,07±0,91 43,40±0,03 -7,79 39,86±0,07 -15,31 Activitatea SOD, un. conv. · g-1 m.p. 25,17±1,15 32,83±0,62 30,43 34,27±0,75 36,15 Activitatea CAT, mM · g-1 m.p. 10,53±0,32 11,45±0,30 8,73 12,22±0,33 16,05 Activitatea APX, mM · g-1 m.p. 7,04±0,28 8,45±0,78 20,02 8,55±0,30 21,45 Activitatea GlPX, mM · g-1 m.p. 69,82±1,80 81,22±0,24 16,33 87,15±1,90 24,83 Activitatea GLR, mM · g-1 m.p. 97,66±3,07 115,05±2,99 17,80 158,11±1,7 61,89 Glycine max (Merr), L. cv. Nadejda Conţinutul DAM, µM · g-1 m. p. 49,90±0,40 42,71±0,04 -14,40 37,92±0,02 -24,00 Activitatea SOD, un. conv. · g-1 m.p. 39,87±1,04 47,71±2,42 19,66 50,82±0,58 27,46 Activitatea CAT, mM · g-1 m. p. 7,72±0,02 9,85±0,42 27,90 11,81±0,58 52,98 Activitatea APX, mM · g-1 m. p. 9,00±0,54 10,62±0,54 18,00 13,02±1,20 44,66 Activitatea GlPX, mM · g-1 m. p. 86,68±1,70 102,14±4,19 17,83 108,71±1,20 25,41 Activitatea GLR, mM · g-1 m. p. 147,95±1,9 156,22±2,72 5,59 161,90±8,02 9,43 Variants Indices Control Thiouree Thiogalmet M ± m M ± m δ, %M* M ± m δ, %M* Zea mays, L. cv. P458 DAM content, µM · g-1 m.p. 47.07±0.91 43.40±0.03 -7.79 39.86±0.07 -15.31 SOD activity, un. conv. · g-1 m.p. 25.17±1.15 32.83±0.62 30.43 34.27±0.75 36.15 CAT activity, mM · g-1 m.p. 10.53±0.32 11.45±0.30 8.73 12.22±0.33 16.05 APX activity, mM · g-1 m.p. 7.04±0.28 8.45±0.78 20.02 8.55±0.30 21.45 GlPX activity, mM · g-1 m.p. 69.82±1.80 81.22±0.24 16.33 87.15±1.90 24.83 GLR activity, mM · g-1 m.p. 97.66±3.07 115.05±2.99 17.80 158.11±1.7 61.89 Glycine max (Merr), L. cv. Nadejda DAM content, µM · g-1 m. p. 49.90±0.40 42.71±0.04 -14.40 37.92±0.02 -24.00 SOD activity, un. conv. · g-1 m.p. 39.87±1.04 47.71±2.42 19.66 50.82±0.58 27.46 CAT activity, mM · g-1 m. p. 7.72±0.02 9.85±0.42 27.90 11.81±0.58 52.98 APX activity, mM · g-1 m. p. 9.00±0.54 10.62±0.54 18.00 13.02±1.20 44.66 Activity GlPX, mM · g-1 m. p. 86.68±1.70 102.14±4.19 17.83 108.71±1.20 25.41 GLR activity, mM · g-1 m. p. 147.95±1, 9 156.22±2.72 5.59 161.90±8.02 9.43
*- comparativ cu varianta martor în aceleaşi condiţii de umiditate insuficientă; m.p. - masă proaspătă *- compared to the control variant under the same conditions of insufficient humidity; m.p. - fresh meal
Rezultatele analizelor au demonstrat că seceta provoacă o majorare a conţinutului DAM în frunzele plantelor netratate de porumb şi soia cu 39,84% şi 51,81% de la valoarea indicelui plantelor martor neexpuse acţiunii secetei. Conţinutul DAM la plantele pretratate conform celei mai apropriate soluţii tehnice şi cu soluţia Tiogalmet în condiţii de secetă moderată se menţinea la un nivel mai mic cu 7,79% şi 15,31% la porumb şi cu 14,40% şi 24,00 % la soia comparativ cu valorile indicelui plantelor martor netratate şi expuse secetei. Conţinutul DAM la plantele pretratate cu soluţii de tiouree şi Tiogalmet pe fond de insuficienţă de umiditate depăşea nivelul DAM a plantelor martor din condiţii optime de umiditate cu 28,93% şi 18,42% la porumb şi cu 29,93% şi 15,36 % la plantele de soia, ceea ce reprezintă un grad de modificare a DAM semnificativ mai mic decât la plantele netratate, dar expuse acţiunii stresului oxidativ cauzat de secetă. Efectul pretratării cu soluţie de Tiogalmet a constituit o reducere a DAM cu 8,88% şi cu 12,63% comparativ cu efectul de la pretratarea conform celei mai apropriate soluţii tehnice. The results of the analyzes demonstrated that the drought causes an increase in the DAM content in the leaves of the untreated corn and soybean plants by 39.84% and 51.81% from the value of the index of the control plants not exposed to the action of the drought. The DAM content in plants pretreated according to the most appropriate technical solution and with the Tiogalmet solution under moderate drought conditions was maintained at a lower level by 7.79% and 15.31% in corn and by 14.40% and 24.00% in soybeans compared to the index values of the control plants untreated and exposed to drought. The DAM content of the plants pretreated with thiourea and Thiogalmet solutions on a background of insufficient moisture exceeded the DAM level of control plants from optimal moisture conditions by 28.93% and 18.42% in corn and by 29.93% and 15.36 % in soybean plants, which represents a significantly lower DAM modification degree than in untreated plants, but exposed to the action of oxidative stress caused by drought. The effect of pretreatment with Tiogalmet solution was a reduction of DAM by 8.88% and by 12.63% compared to the effect of pretreatment according to the most appropriate technical solution.
Efectul de menţinere a stării red-ox a plantelor pretratate se datorează activizării sistemului enzimatic de protecţie antioxidantă a plantelor atât în condiţii optime, cât şi de stres oxidativ (tabelul 3). În condiţii optime de umiditate activitatea enzimelor antioxidante se majorează la plantele de porumb şi soia cu 9,31 şi 8,89 la sută sub influenţa tioureei şi cu 27,51% şi 16,87% respectiv la plantele de porumb şi soia tratate cu soluţie de Tiogalmet. The effect of maintaining the red-ox state of the pretreated plants is due to the activation of the antioxidant protection enzyme system of the plants both under optimal conditions and under oxidative stress (table 3). In optimal humidity conditions, the activity of antioxidant enzymes increases in corn and soybean plants by 9.31 and 8.89 percent under the influence of thiourea and by 27.51% and 16.87%, respectively, in corn and soybean plants treated with the solution by Tiogalmet.
Tabelul 3 Table 3
Conţinutul DAM şi activitatea enzimelor de protecţie antioxidantă la plantele de porumb şi soia în condiţii optime de umiditate şi secetă moderată DAM content and activity of antioxidant protection enzymes in corn and soybean plants under optimal humidity and moderate drought conditions
Variante Indici Martor Tiouree Tiogalmet M ± m δ,%M** M ± m δ, M** M ± m δ, %M** Zea mays, L. cv. P458 Conţinutul DAM, µM· g-1 m.p. optim 33,66±0,24 31,44±0,09 -6,59 24,91±0,04 -25,99 secetă 47,07±0,91 39,84 43,40±0,16 -7,79 39,86±0,07 -15,31 Activitatea enzimatică sumară optim 145,43±8,42 158,97±6,77 9,31 185,44±9,95 27,51 secetă 210,22±6,62 44,50 249,00±6,71 18,44 300,30±4,98 42,85 Glycine max (Merr),L., cv. Nadejda Conţinutul DAM, µM· g-1 m.p. optim 32,87±0,04 25,38±0,06 -22,78 24,67±0,70 -24,95 secetă 49,90±0,40 51,81 42,71±0,04 -14,40 37,92±0,02 -24,00 Activitatea enzimatică sumară optim 203,75±6,45 221,88±6,52 8,89 238,12±6,81 16,87 secetă 291,22±5,28 326,54±6,87 12,13 346,26±9,58 18,89 Variants Indices Control Thiouree Thiogalmet M ± m δ,%M** M ± m δ, M** M ± m δ, %M** Zea mays, L. cv. P458 DAM content, µM· g-1 m.p. optimal 33.66±0.24 31.44±0.09 -6.59 24.91±0.04 -25.99 drought 47.07±0.91 39.84 43.40±0.16 -7 .79 39.86±0.07 -15.31 Optimal summary enzyme activity 145.43±8.42 158.97±6.77 9.31 185.44±9.95 27.51 drought 210.22±6 .62 44.50 249.00±6.71 18.44 300.30±4.98 42.85 Glycine max (Merr), L., cv. Nadejda DAM content, µM· g-1 m.p. optimal 32.87±0.04 25.38±0.06 -22.78 24.67±0.70 -24.95 drought 49.90±0.40 51.81 42.71±0.04 -14 .40 37.92±0.02 -24.00 Optimal summary enzyme activity 203.75±6.45 221.88±6.52 8.89 238.12±6.81 16.87 drought 291.22±5 .28 326.54±6.87 12.13 346.26±9.58 18.89
** - comparativ cu plantele martor în condiţiile corespunzătoare; m.p. - masă proaspătă ** - compared to the control plants under the appropriate conditions; m.p. - fresh meal
În condiţii de secetă intensificarea activităţii enzimelor antioxidante constituie corespunzător 18,44% şi 12,13% la porumb şi soia pretratate cu tiouree şi cu 42,85% şi 18,89 % la cele pretratate cu Tiogalmet. Gradul de modificare a activităţii enzimelor antioxidante asigurat de utilizarea invenţiei comparativ cu cea mai apropriată soluţie tehnică constituie la porumb şi soia în condiţii optime de umiditate 16,65% şi 7,32%, iar în condiţii de secetă 20,60% şi 6,04% corespunzător. Deci, compoziţia propusă permite manifestarea unor proprietăţi antioxidante semnificativ mai bune comparativ cu tioureea. In drought conditions, the intensification of antioxidant enzyme activity is correspondingly 18.44% and 12.13% for corn and soybeans pretreated with thiourea and 42.85% and 18.89% for those pretreated with Thiogalmet. The degree of change in the activity of antioxidant enzymes provided by the use of the invention compared to the most appropriate technical solution is 16.65% and 7.32% for corn and soybeans in optimal humidity conditions, and in drought conditions 20.60% and 6, 04% correspondingly. So, the proposed composition allows the manifestation of significantly better antioxidant properties compared to thiourea.
1. Wahid A., Basra S.M.A., Farooq M. Thiourea: a molecule with immense biological significance for plants. International Journal of Agriculture & Biology, 2017, v. 19, nr. 4, p. 911-920 1. Wahid A., Basra S.M.A., Farooq M. Thiourea: a molecule with immense biological significance for plants. International Journal of Agriculture & Biology, 2017, v. 19, no. 4, pp. 911-920
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210024A MD4818C8 (en) | 2021-04-27 | 2021-04-27 | Composition for cultivated plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210024A MD4818C8 (en) | 2021-04-27 | 2021-04-27 | Composition for cultivated plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD4818B1 MD4818B1 (en) | 2022-08-31 |
| MD4818C1 MD4818C1 (en) | 2023-03-31 |
| MD4818C8 true MD4818C8 (en) | 2023-11-30 |
Family
ID=83112973
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20210024A MD4818C8 (en) | 2021-04-27 | 2021-04-27 | Composition for cultivated plants |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4818C8 (en) |
-
2021
- 2021-04-27 MD MDA20210024A patent/MD4818C8/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| MD4818C1 (en) | 2023-03-31 |
| MD4818B1 (en) | 2022-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sarkar et al. | Validation of ancient liquid organics-Panchagavya and Kunapajala as plant growth promoters | |
| Bacilieri et al. | Titanium (Ti) in plant nutrition-A review | |
| Rajesaheb et al. | Bio-stimulant in improving crop yield and soil health | |
| KR101799780B1 (en) | Method for Manufacturing Plant Nutrient Containing Mineral Element | |
| CN105123330A (en) | Seedling culturing substrate containing Chinese medicine residues and application of seedling culturing substrate in salt golden cypress bonsai seedling culturing | |
| Neocleous | Grafting and silicon improve photosynthesis and nitrate absorption in melon (Cucumis melo L.) plants | |
| LU83853A1 (en) | TITANIUM-CONTAINING COMPOSITIONS FOR PACKAGING PLANTS | |
| CN113207452A (en) | Method for relieving drought stress of grape nursery stocks | |
| MD4818C8 (en) | Composition for cultivated plants | |
| Zhao et al. | Trichoderma combined with 1-aminocyclopropane-1-carboxylic acid (ACC) soil amendments modulates the root microbiome and improves wheat growth under salinity stress | |
| Pippal et al. | Response of guava to foliar spray of zinc, boron and magnesium on growth, development and yield | |
| Skinner et al. | The action of manganese in soils | |
| Meena et al. | Effect of Foliar Spray of Nutrients on Yield and Quality of Nagpur Mandarin (Citrus reticulate Blanco.) | |
| JP2023164255A (en) | Root nodule activity promoter | |
| JP4830134B2 (en) | Foliar spray type nitrate reducing agent | |
| FR2694159A1 (en) | Composition comprising choline, or its derivatives or its salts, and usable in the agricultural field to promote the photosynthesis of plants. | |
| ALTUFAILI et al. | Determination of effectiveness of certain chemical compounds in Lactuca sativa L. under cobalt salt stress | |
| Gamit et al. | Effect of integrated nutrient management on growth and yield attributes of cluster bean under South Gujarat conditions | |
| Naz et al. | Exploring the potential of silicon and salicylic acid in the alleviation of water deficit stress in quinoa (Chenopodium quinoa) | |
| CN106561361A (en) | Selenium-enrichment rice cultivation technology | |
| Ghafarizadeh et al. | Synergistic effect of seaweed extract and urea fertilizer on growth and biochemical parameters of Triticum aestivum at vegetative stage | |
| CN106508534A (en) | Method of cultivating rice rich in selenium | |
| Meena et al. | Effect of foliar spray of micronutrients on growth attributes of Nagpur mandarin (Citrus reticulata Blanco.) | |
| Selvaraj et al. | Role of Panchagavya on growth and health of vegetable crops | |
| Dadasoglu et al. | Mitigation of salt stress in Phaseolus vulgaris L. by dopamine: Effects on growth, physiological parameters and antioxidant activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| TC4A | Change of name of proprietor (patent for invention) |
Owner name: INSTITUTIA PUBLICA UNIVERSITATEA DE STAT DIN MOLDOVA, MD STR. A. MATEEVICI NR. 60, MD-2009, CHISINAU, REPUBLICA MOLDOVA Free format text: PREVIOUS NAME OF PROPRIETOR: INSTITUTUL DE CHIMIE, MEC, MD STR. ACADEMIEI NR. 3, MD-2028, REPUBLICA MOLDOVAINSTITUTUL DE GENETICA, FIZIOLOGIE SI PROTECTIE A PLANTELOR AL MEC, MD STR. PADURII NR. 20, MD-2002, REPUBLICA MOLDOVA |