MD293Z - Use of water-soluble oenotannin for the treatment of gastric and duodenal ulcer - Google Patents
Use of water-soluble oenotannin for the treatment of gastric and duodenal ulcer Download PDFInfo
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- MD293Z MD293Z MDS20100138A MDS20100138A MD293Z MD 293 Z MD293 Z MD 293Z MD S20100138 A MDS20100138 A MD S20100138A MD S20100138 A MDS20100138 A MD S20100138A MD 293 Z MD293 Z MD 293Z
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- Moldova
- Prior art keywords
- treatment
- water
- soluble
- oenotannin
- gastric
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- 238000011282 treatment Methods 0.000 title claims abstract description 13
- 208000007107 Stomach Ulcer Diseases 0.000 title claims abstract description 5
- 208000000718 duodenal ulcer Diseases 0.000 title claims abstract description 5
- 230000002496 gastric effect Effects 0.000 title claims abstract description 5
- 201000005917 gastric ulcer Diseases 0.000 title abstract 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000001590 oxidative effect Effects 0.000 claims description 4
- 208000008469 Peptic Ulcer Diseases 0.000 abstract description 6
- 231100000029 gastro-duodenal ulcer Toxicity 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 4
- 230000004071 biological effect Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 208000025865 Ulcer Diseases 0.000 description 14
- 231100000397 ulcer Toxicity 0.000 description 11
- 241000700159 Rattus Species 0.000 description 5
- 230000000767 anti-ulcer Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- CGIGDMFJXJATDK-UHFFFAOYSA-N indomethacin Chemical compound CC1=C(CC(O)=O)C2=CC(OC)=CC=C2N1C(=O)C1=CC=C(Cl)C=C1 CGIGDMFJXJATDK-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 208000007882 Gastritis Diseases 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000219095 Vitis Species 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 229960000905 indomethacin Drugs 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010009900 Colitis ulcerative Diseases 0.000 description 1
- 206010061164 Gastric mucosal lesion Diseases 0.000 description 1
- 206010017886 Gastroduodenal ulcer Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 201000006704 Ulcerative Colitis Diseases 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000016571 aggressive behavior Effects 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 229920002770 condensed tannin Polymers 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229940038487 grape extract Drugs 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 206010034754 petechiae Diseases 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
Invenţia se referă la medicină, în special la gastroenterologie şi poate fi utilizată pentru tratamentul ulcerelor gastroduodenale. The invention relates to medicine, in particular to gastroenterology and can be used for the treatment of gastroduodenal ulcers.
În tratamentul ulcerelor gastroduodenale se utilizează frecvent substanţe astringente, regeneratoare şi antiinflamatoare din diverse grupe, o largă răspândire şi o eficacitate înaltă având în special substanţele tanante [1]. In the treatment of gastroduodenal ulcers, astringent, regenerating and anti-inflammatory substances from various groups are frequently used, with tanning substances being particularly widespread and highly effective [1].
Dezavantajul este că majoritatea plantelor cu conţinut ridicat de taninuri cresc în pădurile tropicale, astfel materia primă este inaccesibilă, costisitoare şi procedeele de obţinere sunt scumpe. The disadvantage is that most plants with high tannin content grow in tropical forests, so the raw material is inaccessible, expensive and the production processes are expensive.
Se cunoaşte, de asemenea, utilizarea enotaninului hidrosolubil obţinut la oxidarea enotaninului cu peroxid de hidrogen în calitate de remediu antibacterian [2]. The use of water-soluble enotanin obtained by oxidizing enotanin with hydrogen peroxide as an antibacterial remedy is also known [2].
Problema pe care o rezolvă invenţia dată constă în elaborarea unei metode mai eficiente de tratament al gastritelor, ulcerelor gastrice şi duodenale. The problem solved by this invention consists in developing a more efficient method for treating gastritis, gastric and duodenal ulcers.
Esenţa invenţiei constă în utilizarea enotaninului hidrosolubil, obţinut la oxidarea enotaninului cu peroxid de hidrogen, pentru tratamentul ulcerului gastric şi duodenal. The essence of the invention consists in the use of water-soluble enotanin, obtained by oxidizing enotanin with hydrogen peroxide, for the treatment of gastric and duodenal ulcers.
Rezultatul invenţiei constă în utilizarea unui remediu mai eficient pentru tratamentul ulcerelor gastroduodenale prin utilizarea unei substanţe de origine vegetală cu activitate biologică înaltă. The result of the invention consists in the use of a more effective remedy for the treatment of gastroduodenal ulcers by using a substance of plant origin with high biological activity.
Datorită obţinerii enotaninului hidrosolubil din seminţe de struguri prin oxidarea enotaninului, putem beneficia de un remediu cu proprietăţi astringente, antiinflamatoare şi regeneratoare ridicate. Due to the extraction of water-soluble enotannin from grape seeds by oxidizing enotannin, we can benefit from a remedy with high astringent, anti-inflammatory and regenerative properties.
Avantajele invenţiei constau în utilizarea unui remediu de origine vegetală cu activitate biologică înaltă, obţinut din materie primă autohtonă - deşeuri ale industriei vinicole, în calitate de substanţă cu activitate antiulceroasă, la un preţ accesibil. Enotaninul hidrosolubil şi-a dovedit eficacitatea în studiile efectuate pe plăgi şi arsuri, ca fiind un preparat regenerator efectiv şi cu o activitate antibacteriană înaltă. Datorită hidrosolubilităţii enotaninul poate fi utilizat pe larg sub formă de soluţii. The advantages of the invention consist in the use of a remedy of plant origin with high biological activity, obtained from local raw material - waste from the wine industry, as a substance with antiulcer activity, at an affordable price. Water-soluble enotanin has proven its effectiveness in studies conducted on wounds and burns, as an effective regenerative preparation and with high antibacterial activity. Due to its water solubility, enotanin can be widely used in the form of solutions.
Experimentele au fost efectuate pe 20 şobolani albi cu masa corporală cuprinsă între 180...220 g. Modelarea ulcerelor a fost efectuată conform metodei (Derelanko M.G.), prin administrarea unei singure doze intragastrale de indometacină (20 mg/kg). Studiul a fost efectuat pe 2 loturi, la care s-a administrat ca tratament enotaninul hidrosolubil şi soluţie fiziologică (lotul martor). Durata tratamentului a constituit 5 zile depline începând cu a doua zi după administrarea indometacinei. The experiments were performed on 20 white rats with a body mass between 180...220 g. Ulcer modeling was performed according to the method (Derelanko M.G.), by administering a single intragastric dose of indomethacin (20 mg/kg). The study was performed on 2 groups, which were administered as treatment water-soluble enotanin and physiological solution (control group). The duration of treatment was 5 full days starting with the second day after indomethacin administration.
Stabilirea numărului şi a suprafeţei ulcerelor s-a efectuat după sacrificarea animalelor prin decapitare, cu izolarea ulterioară a stomacului, fiind preventiv anesteziaţi cu eter. Pentru a stabili starea iniţială, s-au sacrificat câte doi şobolani din fiecare lot peste 24 ore, iar pentru a stabili eficacitatea tratamentului timp de 5 zile - la a 6-a zi. Macroscopic s-a determinat numărul de ulcere, suprafaţa şi procentul şobolanilor cu ulcer. Activitatea terapeutică antiulceroasă s-a apreciat comparativ cu lotul martor prin determinarea indicelui ulceros. The number and surface area of ulcers were determined after the animals were sacrificed by decapitation, with subsequent isolation of the stomach, being preventively anesthetized with ether. To establish the initial state, two rats from each group were sacrificed after 24 hours, and to establish the effectiveness of the treatment for 5 days - on the 6th day. The number of ulcers, the surface area and the percentage of rats with ulcers were determined macroscopically. The antiulcer therapeutic activity was assessed in comparison with the control group by determining the ulcer index.
Suprafaţa totală a ulcerelor la un şobolan după 24 ore a fost în medie de 1,42±2,03 mm2, iar numărul mediu de ulcere era egal cu 10,5±1,39 la un animal. După 5 zile de tratament s-a constatat vindecarea ulcerelor la majoritatea animalelor în toate loturile comparativ cu starea iniţială, după 24 ore. Evoluţia cea mai benefică a ulcerului a fost determinată în lotul cu administrarea enotaninului, care a fost statistic veridică comparativ cu lotul martor. Utilizarea enotaninului hidrosolubil reduce suprafaţa şi numărul ulcerelor până la 2,2 ± 0,45 mm2 contribuind la o vindecare mai rapidă şi efectivă. The total area of ulcers in a rat after 24 hours was on average 1.42±2.03 mm2, and the average number of ulcers was equal to 10.5±1.39 per animal. After 5 days of treatment, healing of ulcers was observed in most animals in all groups compared to the initial state after 24 hours. The most beneficial evolution of ulcers was determined in the group with enotanin administration, which was statistically significant compared to the control group. The use of water-soluble enotanin reduces the area and number of ulcers to 2.2 ± 0.45 mm2, contributing to faster and more effective healing.
Analiza datelor experimentale obţinute a constatat o scădere cu 50% a suprafeţei ulceroase şi de aproximativ două ori a numărului leziunilor stomacale comparativ cu lotul martor, ceea ce ne demonstrează înalta activitate antiulceroasă a enotaninului hidrosolubil. Analysis of the experimental data obtained found a 50% decrease in the ulcer surface and approximately two times the number of stomach lesions compared to the control group, which demonstrates the high antiulcer activity of water-soluble enotanin.
Efectul antiulceros al extractului de struguri poate fi explicat prin multitudinea proprietăţilor farmacologice pe care le posedă taninurile. Acţiunea astringentă este cea mai importantă, ea se manifestă prin precipitarea proteinelor din plagă cu formarea unei pelicule proteice dense, impermeabile pentru apă şi alţi factori de agresie externi ca acidul clorhidric, enzimele digestive, protejând astfel ţesuturile subiacente la nivelul leziunii ulceroase. De asemenea, ca urmare a coagulării proteinelor are loc oprirea hemoragiilor capilare din leziunile ulceroase. Un alt efect este cel antiinflamator care se manifestă în faza acută a inflamaţiei prin scăderea răspunsului inflamator, inhibarea infiltraţiei celulare, ameliorarea stresului oxidativ şi micşorarea producerii mediatorilor inflamaţiei. The antiulcer effect of grape extract can be explained by the multitude of pharmacological properties that tannins possess. The astringent action is the most important, it manifests itself by the precipitation of proteins in the wound with the formation of a dense protein film, impermeable to water and other external aggression factors such as hydrochloric acid, digestive enzymes, thus protecting the underlying tissues at the level of the ulcerative lesion. Also, as a result of the coagulation of proteins, capillary hemorrhages from ulcerative lesions stop. Another effect is the anti-inflammatory one which manifests itself in the acute phase of inflammation by decreasing the inflammatory response, inhibiting cellular infiltration, improving oxidative stress and reducing the production of inflammatory mediators.
Studiul efectuat în laboratorul şi catedra universităţii stabileşte şi confirmă că enotaninul hidrosolubil contribuie la stimularea regenerării leziunilor mucoasei gastrice exercitând o acţiune antiulceroasă semnificativă şi cu o înaltă siguranţă de utilizare. Astfel el poate fi recomandat în schemele de tratament la pacienţii cu ulcer gastroduodenal şi alte afecţiuni gastroenterologice ulcerative, precum şi în gastrite. The study conducted in the university laboratory and department establishes and confirms that water-soluble enotanin contributes to the stimulation of the regeneration of gastric mucosal lesions, exerting a significant antiulcer action and with a high safety of use. Thus, it can be recommended in treatment regimens for patients with gastroduodenal ulcer and other ulcerative gastroenterological diseases, as well as gastritis.
1. Li XL, Cai UQ, Qin U., Wu YJ. Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNDS-inducted ulcerative colitis. Can J. Physiol. Pharmacol., 2008, № 86(12), p.841-849 1. Li XL, Cai UQ, Qin U., Wu YJ. Therapeutic effect and mechanism of proanthocyanidins from grape seeds in rats with TNDS-induced ulcerative colitis. Can J. Physiol. Pharmacol., 2008, № 86(12), p.841-849
2. MD 3228 G2 2007.01.31 2. MD 3228 G2 2007.01.31
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20100138A MD293Z (en) | 2009-12-21 | 2009-12-21 | Use of water-soluble oenotannin for the treatment of gastric and duodenal ulcer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20100138A MD293Z (en) | 2009-12-21 | 2009-12-21 | Use of water-soluble oenotannin for the treatment of gastric and duodenal ulcer |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD293Y MD293Y (en) | 2010-11-30 |
| MD293Z5 MD293Z5 (en) | 2011-07-31 |
| MD293Z true MD293Z (en) | 2011-07-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20100138A MD293Z (en) | 2009-12-21 | 2009-12-21 | Use of water-soluble oenotannin for the treatment of gastric and duodenal ulcer |
Country Status (1)
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Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2074729C1 (en) * | 1993-03-03 | 1997-03-10 | Сибирский государственный медицинский институт | Medicinal agent for ulcer stomach and duodenum treatment |
| JPH10218769A (en) * | 1997-02-06 | 1998-08-18 | Kikkoman Corp | Antiulcer agent |
| MD869G2 (en) * | 1997-03-24 | 1998-10-31 | Национальный Институт Винограда И Вина | Process for preparation of tannins and accompanying products from the grape seeds |
| MD1140C2 (en) * | 1997-07-15 | 1999-06-30 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Remedy possessing a regenerative and cytoprotective activity |
| MD2070G2 (en) * | 2002-07-12 | 2003-06-30 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Utilization of the unrefined grapestone oil in the treatment of gastric and duodenal ulcers |
| MD3125G2 (en) * | 2005-12-06 | 2007-05-31 | Институт Химии Академии Наук Молдовы | Process for obtaining water-soluble enotanins |
| MD3228G2 (en) * | 2006-03-07 | 2007-08-31 | Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова | Antibacterial agent |
| KR100851962B1 (en) * | 2006-11-13 | 2008-08-12 | 주식회사 케이씨에프코리아 | Composition for anti-helicobacter pylori containing grape seed extract |
-
2009
- 2009-12-21 MD MDS20100138A patent/MD293Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD293Z5 (en) | 2011-07-31 |
| MD293Y (en) | 2010-11-30 |
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