CN101704853A - Extraction and purification method of amygdalin in Cerasus humilis kernel - Google Patents
Extraction and purification method of amygdalin in Cerasus humilis kernel Download PDFInfo
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- CN101704853A CN101704853A CN200910230090A CN200910230090A CN101704853A CN 101704853 A CN101704853 A CN 101704853A CN 200910230090 A CN200910230090 A CN 200910230090A CN 200910230090 A CN200910230090 A CN 200910230090A CN 101704853 A CN101704853 A CN 101704853A
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Abstract
The invention belongs to the technical field of medical food, and discloses an extraction and purification method of amygdalin in Cerasus humilis kernel. The adopted purification method is simple and effective and can extract the amygdalin in Cerasus humilis kernel to the largest extent. The obtained amygdalin not only can be used for relieving cough and resisting inflammatory, but also can be used for treating tumor, thus the method of the invention has wide application prospect in the pharmaceutical field.
Description
Technical field:
The present invention relates to a kind of method of purification, be specifically related to extraction, the purification process of amygdaloside among a kind of Europe Li Ren, belong to the medicine food technical field.
Background technology:
Europe Lee is the distinctive rose department cherry genus shrub species of China, and is wild, mainly is distributed in Shanxi, Hebei, Shaanxi, Inner Mongol and the Northeast of central and west regions.Europe Lee's economic worth is high, and purposes is very extensive.Pulp is edible, and benevolence can be used as medicine, the capable of using as feed and braided material of stem, and its exploitation prospect is boundless.
Amygdaloside has good pharmaceutical use, extensively is present in the seed of multiple rosaceous plant fruits such as apricot, peach, plum, apple, hawthorn, loquat.The amygdaloside molecular structure is made up of a unit phenyl aldehyde, a unit prussic acid and two glucose unit.Amygdaloside has good antitumor action, is used as the ancillary drug for the treatment of cancer.The amygdaloside preparation is used for the treatment of patient with advanced cancer, can makes doing well,improving, survival time prolongs.Amygdaloside is used for the treatment of cancerous hydrothorax, finds that amygdaloside has to a certain degree control and mitigation to cancerous hydrothorax.Amygdaloside is 50%～70% to the inhibiting rate of cervical cancer JJC26 strain.Amygdaloside and prussic acid and phenyl aldehyde in vitro tests that hydrolysis produced all have been proved to be antitumous effect, and the normal cell of sulfhydrase is few in the cancer cells, and therefore, it is relatively poor that hydrolysis discharges the detoxification ability of prussic acid to amygdaloside.Amygdaloside adds phenyl aldehyde or amygdaloside and adds beta-glucosidase enzyme and can obviously improve anticancer effectiveness, found that cancer cells anaerobic glycolysis preponderates, the slant acidity environment that its product lactic acid forms helps improving the activity of beta-glucosidase enzyme, impels amygdaloside hydrolysis in cancer cells to go out more prussic acid and phenyl aldehyde and brings into play stronger antitumous effect.
Discover that amygdaloside can obviously improve the vigor of Terminal oxidase under the cerebral ischemic condition, improves energy metabolism, prolong the cells survival time, promote histocyte metabolism and functional rehabilitation and tissue repair.Amygdaloside and actovegin (are treated one of active drug of cerebral ischemia relative disease at present, it is calf blood essence extract, can promote extraction and the utilization of hypoxic cell to oxygen and glucose, improve energy metabolism, also have antithrombotic to form and promotion healing effect) the same, belong to a kind of brain cell metabolism activating agent.
But existing amygdaloside extracting method is comparatively complicated, and wherein the effective medicinal components utilization ratio is low, can't reach the extraction purpose of expection, and can cause damage to amygdaloside in the leaching process, has reduced its purity, can't directly utilize.
Summary of the invention:
The weak point that exists on the extraction and purification method at amygdaloside among the existing Ou Liren, the invention provides the extraction and purification method of amygdaloside among a kind of brand-new Ou Liren, mainly with resin Europe Lee's fruit stone being carried out post purifies, adopt this method, as solvent, reduced the application of organic solvent in whole purification process with pure water, prevented the influence of organic solvent final product, the amygdaloside that is obtained not only can be used for antibechic and anti-inflammatory, can also be used for the treatment of tumour.Therefore, in pharmaceutical field, be with a wide range of applications.
The extraction and purification method of amygdaloside among the Europe Li Ren of the present invention, its step is as follows:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder;
Add Europe Lee's powder and pure water in the insoluble container in water-bath, 2-4h is extracted in heating, filters, and gets extracting solution;
Adopt HZ801 or AB-8 or D101 resin to the amygdaloside purifying, preferred resin is HZ801; Upper prop liquid is said extracted liquid, is elutriant with the aqueous ethanolic solution of 20-50%, and elution flow rate is 2-6BV/h, and the elutriant consumption is 2-3BV.
Through the extracting solution after hot water extraction and the filtration, its amygdaloside concentration is 1.0-1.5g/L, owing to adopt pure water to extract, the consumption and the solvent that have reduced organic solvent are easy to get, and can not cause extra pollution, and final extraction efficiency is higher, can use the preferred heating extraction 2h that adopts widely.
Adopt HZ801 or AB-8 or D101 resin that extracting solution is carried out column purification, can effectively amygdaloside be extracted from water, simpler and easy to operate than existing mode, the final ethanol that adopts is as elutriant, can reduce the cost of extraction greatly, reduce pollution simultaneously, preferably adopt 40% aqueous ethanolic solution as elutriant for environment.Finally to operating by wash-out, the eluting rate of amygdaloside can reach more than 70%, far above existing various methods of purification.
Adopt this method, as solvent, reduced the application of organic solvent in whole purification process with pure water, prevented the influence of organic solvent to final product, the amygdaloside that is obtained not only can be used for antibechic and anti-inflammatory, can also be used for the treatment of tumour.Therefore, in pharmaceutical field, be with a wide range of applications.
Embodiment:
Understand technical scheme of the present invention for better, below describe in further detail by specific embodiment.
Embodiment 1:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder; Add 6g Europe Lee's powder and 60g pure water in the insoluble container in 60 ℃ of water-baths, heating was extracted 2 hours; Filter, get extracting solution; Adopt HZ801 type resin to the amygdaloside purifying, upper prop liquid is said extracted liquid, and the aqueous ethanolic solution with 30% is an elutriant, and elution flow rate is 2BV/h, and the elutriant consumption is 2.5BV.The eluting rate of amygdaloside is 72.58%.
Embodiment 2:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder; Add 6g Europe Lee's powder and 60g pure water in the insoluble container in 60 ℃ of water-baths, heating was extracted 2 hours; Filter, get extracting solution; Adopt AB-8 type resin to the amygdaloside purifying, upper prop liquid is said extracted liquid, and the aqueous ethanolic solution with 40% is an elutriant, and elution flow rate is 2BV/h, and the elutriant consumption is 2.5BV.The eluting rate of amygdaloside is 98.76%.
Embodiment 3:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder; Add 6g Europe Lee's powder and 60g pure water in the insoluble container in 60 ℃ of water-baths, heating was extracted 2 hours; Filter, get extracting solution; Adopt HZ801 type resin to the amygdaloside purifying, upper prop liquid is said extracted liquid, and the aqueous ethanolic solution with 50% is an elutriant, and elution flow rate is 2BV/h, and the elutriant consumption is 2.5BV.The eluting rate of amygdaloside is 93.39%.
Embodiment 4:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder; Add 6g Europe Lee's powder and 60g pure water in the insoluble container in 60 ℃ of water-baths, heating was extracted 2 hours; Filter, get extracting solution; Adopt HZ801 type resin to the amygdaloside purifying, upper prop liquid is said extracted liquid, and the aqueous ethanolic solution with 40% is an elutriant, and elution flow rate is 4BV/h, and the elutriant consumption is 2.5BV.The eluting rate of amygdaloside is 91.65%.
Embodiment 5:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder; Add 6g Europe Lee's powder and 60g pure water in the insoluble container in 60 ℃ of water-baths, heating was extracted 2 hours; Filter, get extracting solution; Adopt D101 type resin to the amygdaloside purifying, upper prop liquid is said extracted liquid, and the aqueous ethanolic solution with 40% is an elutriant, and elution flow rate is 6BV/h, and the elutriant consumption is 2.5BV.The eluting rate of amygdaloside is 83.26%.
Claims (4)
1. the extraction and purification method of amygdaloside among the Ou Liren, it is characterized in that: its step is as follows:
Europe Lee's fruit stone is ground into 10-40 purpose meal, is prepared into Europe Lee's powder;
Add Europe Lee's powder and pure water in the insoluble container in water-bath, 2-4h is extracted in heating, filters, and gets extracting solution;
Adopt HZ801 or AB-8 or D101 resin to the amygdaloside purifying; Upper prop liquid is said extracted liquid, is elutriant with the aqueous ethanolic solution of 20-50%, and elution flow rate is 2-6BV/h, and the elutriant consumption is 2-3BV.
2. extraction and purification method according to claim 1 is characterized in that: the extracting solution after the described filtration, its amygdaloside concentration is 1.0-1.5g/L.
3. extraction and purification method according to claim 1 and 2 is characterized in that: described resin is preferably the HZ801 resin.
4. extraction and purification method according to claim 1 and 2 is characterized in that: described elution flow rate is 2-4BV/h, and the elutriant consumption is 2.5BV.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910230090A CN101704853A (en) | 2009-11-13 | 2009-11-13 | Extraction and purification method of amygdalin in Cerasus humilis kernel |
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| CN200910230090A CN101704853A (en) | 2009-11-13 | 2009-11-13 | Extraction and purification method of amygdalin in Cerasus humilis kernel |
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| CN101704853A true CN101704853A (en) | 2010-05-12 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399622A (en) * | 2010-09-09 | 2012-04-04 | 北京市农林科学院 | Method for preparing bitter almond extract product |
| CN102399623A (en) * | 2010-09-09 | 2012-04-04 | 北京市农林科学院 | Method for preparing Cerasus humilis kernel extract |
| CN102702282A (en) * | 2012-06-04 | 2012-10-03 | 西安天丰生物科技有限公司 | Method for separating amygdalin from almond oil residue |
| CN103214529A (en) * | 2013-05-10 | 2013-07-24 | 浙江省农业科学院 | Method for extracting amygdalin from kernel of red bayberry fruit |
| CN107759644A (en) * | 2017-10-24 | 2018-03-06 | 陕西师范大学 | A kind of method that Amberlyst process recovery semen armeniacae amarae takes off amarogentin in misery |
-
2009
- 2009-11-13 CN CN200910230090A patent/CN101704853A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102399622A (en) * | 2010-09-09 | 2012-04-04 | 北京市农林科学院 | Method for preparing bitter almond extract product |
| CN102399623A (en) * | 2010-09-09 | 2012-04-04 | 北京市农林科学院 | Method for preparing Cerasus humilis kernel extract |
| CN102399623B (en) * | 2010-09-09 | 2013-05-08 | 北京市农林科学院 | Method for preparing Cerasus humilis kernel extract |
| CN102702282A (en) * | 2012-06-04 | 2012-10-03 | 西安天丰生物科技有限公司 | Method for separating amygdalin from almond oil residue |
| CN102702282B (en) * | 2012-06-04 | 2014-11-26 | 西安天丰生物科技有限公司 | Method for separating amygdalin from almond oil residue |
| CN103214529A (en) * | 2013-05-10 | 2013-07-24 | 浙江省农业科学院 | Method for extracting amygdalin from kernel of red bayberry fruit |
| CN107759644A (en) * | 2017-10-24 | 2018-03-06 | 陕西师范大学 | A kind of method that Amberlyst process recovery semen armeniacae amarae takes off amarogentin in misery |
| CN107759644B (en) * | 2017-10-24 | 2020-04-17 | 陕西师范大学 | Method for recovering amygdalin in debitterized water of bitter almonds by macroporous resin method |
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Application publication date: 20100512 |