MD2102G2 - Process for obtaining of tetrooside [(25R)-5a-furostan-3b, 22a, 26-triol]-26-O-b-D-glycopyranoside - Google Patents
Process for obtaining of tetrooside [(25R)-5a-furostan-3b, 22a, 26-triol]-26-O-b-D-glycopyranoside Download PDFInfo
- Publication number
- MD2102G2 MD2102G2 MDA20010329A MD20010329A MD2102G2 MD 2102 G2 MD2102 G2 MD 2102G2 MD A20010329 A MDA20010329 A MD A20010329A MD 20010329 A MD20010329 A MD 20010329A MD 2102 G2 MD2102 G2 MD 2102G2
- Authority
- MD
- Moldova
- Prior art keywords
- obtaining
- furostan
- glycopyranoside
- triol
- chloroform
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- WFDIJRYMOXRFFG-UHFFFAOYSA-N Acetic anhydride Chemical compound CC(=O)OC(C)=O WFDIJRYMOXRFFG-UHFFFAOYSA-N 0.000 abstract 3
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 abstract 3
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 abstract 2
- 239000003480 eluent Substances 0.000 abstract 2
- 240000007377 Petunia x hybrida Species 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 230000021736 acetylation Effects 0.000 abstract 1
- 238000006640 acetylation reaction Methods 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- UXTMROKLAAOEQO-UHFFFAOYSA-N chloroform;ethanol Chemical compound CCO.ClC(Cl)Cl UXTMROKLAAOEQO-UHFFFAOYSA-N 0.000 abstract 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000005238 degreasing Methods 0.000 abstract 1
- 239000012467 final product Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 abstract 1
- 238000007127 saponification reaction Methods 0.000 abstract 1
- 239000000741 silica gel Substances 0.000 abstract 1
- 229910002027 silica gel Inorganic materials 0.000 abstract 1
- 238000010898 silica gel chromatography Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 229930002534 steroid glycoside Natural products 0.000 abstract 1
Landscapes
- Steroid Compounds (AREA)
Abstract
The invention refers to the bio-organic chemistry, in particular to a process for obtaining of tetraoside[(25R)-5α-furostan-3β,22α,26-triol]-26-O-β-D-glycopyranoside, manifesting gibberellenic activity.The process includes degreasing of Petunia hybrida hort. seeds, obtaining of extract thereof with hydroalcoholic solution, concentration of the extract, silica gel column chromatography of the obtained residue, using in the capacity of eluent the chloroform-methanol-water system in the ratio of 60:30:10…65:35:10, acetylation of the steroid glycoside fractions with acetic anhydride in pyridine, chromatography of the obtained derivatives onto a column with silica gel impregnated with 15% AgNO3 aqueous solution in the chloroform-ethanol eluents system in the ratio 3:1…45:46 and saponification of the separated paracetate with the subsequent isolation of the final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20010329A MD2102G2 (en) | 2001-10-01 | 2001-10-01 | Process for obtaining of tetrooside [(25R)-5a-furostan-3b, 22a, 26-triol]-26-O-b-D-glycopyranoside |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20010329A MD2102G2 (en) | 2001-10-01 | 2001-10-01 | Process for obtaining of tetrooside [(25R)-5a-furostan-3b, 22a, 26-triol]-26-O-b-D-glycopyranoside |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD2102F1 MD2102F1 (en) | 2003-02-28 |
| MD2102G2 true MD2102G2 (en) | 2003-08-31 |
Family
ID=19739974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20010329A MD2102G2 (en) | 2001-10-01 | 2001-10-01 | Process for obtaining of tetrooside [(25R)-5a-furostan-3b, 22a, 26-triol]-26-O-b-D-glycopyranoside |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD2102G2 (en) |
-
2001
- 2001-10-01 MD MDA20010329A patent/MD2102G2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD2102F1 (en) | 2003-02-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |