MD1180Z - Method for stimulating the formation of conditioned reflexes in the period of diminution of functions - Google Patents
Method for stimulating the formation of conditioned reflexes in the period of diminution of functions Download PDFInfo
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- MD1180Z MD1180Z MDS20170030A MDS20170030A MD1180Z MD 1180 Z MD1180 Z MD 1180Z MD S20170030 A MDS20170030 A MD S20170030A MD S20170030 A MDS20170030 A MD S20170030A MD 1180 Z MD1180 Z MD 1180Z
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- 230000001143 conditioned effect Effects 0.000 title claims abstract description 29
- 230000011514 reflex Effects 0.000 title claims abstract description 28
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000004936 stimulating effect Effects 0.000 title claims abstract description 9
- 230000006870 function Effects 0.000 title claims description 6
- 239000002028 Biomass Substances 0.000 claims abstract description 18
- 241000187438 Streptomyces fradiae Species 0.000 claims abstract description 12
- 239000002207 metabolite Substances 0.000 claims abstract description 12
- 230000037396 body weight Effects 0.000 claims abstract description 5
- 230000003292 diminished effect Effects 0.000 claims description 4
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 abstract description 2
- 235000015872 dietary supplement Nutrition 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 19
- 241000700159 Rattus Species 0.000 description 10
- 230000000694 effects Effects 0.000 description 10
- 235000013305 food Nutrition 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 6
- 238000011835 investigation Methods 0.000 description 6
- 241000187747 Streptomyces Species 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 241000700157 Rattus norvegicus Species 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 239000004471 Glycine Substances 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 229930182558 Sterol Natural products 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000003704 aspartic acid Nutrition 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000008717 functional decline Effects 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 150000003432 sterols Chemical class 0.000 description 2
- 235000003702 sterols Nutrition 0.000 description 2
- 150000003626 triacylglycerols Chemical class 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 241000946786 Kitasatospora purpeofusca Species 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- 241001494960 Streptomyces griseoflavus Species 0.000 description 1
- 241000946871 Streptomyces massasporeus Species 0.000 description 1
- 241001137869 Streptomyces nitrosporeus Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- 230000037328 acute stress Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 150000001510 aspartic acids Chemical class 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000002307 glutamic acids Chemical class 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000004112 neuroprotection Effects 0.000 description 1
- 230000000324 neuroprotective effect Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 230000003040 nociceptive effect Effects 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
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Abstract
Изобретение относится к экспериментальной физиологии, экспериментальной медицине и фармакологии, в частности к способам стимулирования формирования условных рефлексов в период снижения функций.Согласно изобретению, способ включает введение в качестве пищевой добавки метаболитов биомассы штамма Streptomyces fradiae CNMN-Ac-11, в дозе 250 мг/кг массы тела в сутки, в течение 90 суток до формирования и в период формирования условных рефлексов.The invention relates to experimental physiology, experimental medicine and pharmacology, in particular to methods for stimulating the formation of conditioned reflexes during a period of reduced function. According to the invention, the method includes the introduction of a Streptomyces fradiae CNMN-Ac-11 strain biomass metabolite as a food supplement, at a dose of 250 mg / kg body weight per day, for 90 days before the formation and during the formation of conditioned reflexes.
Description
Invenţia se referă la fiziologia experimentală, medicina experimentală şi farmacologie, în special la procedee de stimulare a formării reflexelor condiţionate în perioada diminuării funcţiilor. The invention relates to experimental physiology, experimental medicine and pharmacology, in particular to methods of stimulating the formation of conditioned reflexes during the period of diminished functions.
Este cunoscută aplicarea metaboliţilor biologic activi din biomasa unor tulpini de streptomicete, de exemplu a estifoeninelor A şi B, produse de Streptomyces purpeofuscus [1], benzostatinelor H şi I, separate din biomasa Streptomyces nitrosporeus [2], mescengricinei extrase din biomasa Streptomyces griseoflavus 2853-SVS4 [3] cu scopul stimulării activităţii nervoase, acţiunii asupra activităţii celulelor nervoase, cât şi a structurilor encefalului, responsabile de procesele de memorare, învăţare şi neuroprotecţie. The application of biologically active metabolites from the biomass of some strains of streptomycetes is known, for example, estiphoenins A and B, produced by Streptomyces purpeofuscus [1], benzostatins H and I, separated from the biomass of Streptomyces nitrosporeus [2], mescengricin extracted from the biomass of Streptomyces griseoflavus 2853-SVS4 [3] with the aim of stimulating nervous activity, acting on the activity of nerve cells, as well as the structures of the brain, responsible for the processes of memory, learning and neuroprotection.
Cea mai apropiată soluţie a invenţiei propuse este procedeul de stimulare a formării reflexelor condiţionate la şobolanii albi, care include utilizarea metaboliţilor din biomasa şi mediul nutritiv al tulpinii Streptomyces messasporeus CNMN-Ac-36 în doză de 250 mg/kg şi 1…2 ml/kg de mediu nutritiv. Tulpina este eliminată din solurile Moldovei şi depozitată în Colecţia Naţională a Microorganismelor Nepatogene a AŞM [4]. The closest solution to the proposed invention is the process of stimulating the formation of conditioned reflexes in white rats, which includes the use of metabolites from the biomass and nutrient medium of the Streptomyces messasporeus CNMN-Ac-36 strain in a dose of 250 mg/kg and 1…2 ml/kg of nutrient medium. The strain is removed from the soils of Moldova and stored in the National Collection of Nonpathogenic Microorganisms of the ASM [4].
Totuşi, în cea mai apropiată soluţie, nu au fost realizate investigaţii referitor la influenţa metaboliţilor Streptomyces messasporeus CNMN-36 asupra formării reflexelor condiţionate la animalele senile (în perioada diminuării funcţiilor). However, in the closest solution, no investigations have been conducted regarding the influence of Streptomyces messasporeus CNMN-36 metabolites on the formation of conditioned reflexes in senile animals (during the period of functional decline).
Problema tehnică pe care o soluţionează invenţia constă în elaborarea unui procedeu de stimulare a formării reflexelor condiţionate în perioada diminuării funcţiilor, care ar permite optimizarea procesului de formare a reflexelor condiţionate la animale, îndeosebi în perioada diminuării funcţiilor. The technical problem solved by the invention consists in developing a method for stimulating the formation of conditioned reflexes during the period of diminished functions, which would allow optimizing the process of forming conditioned reflexes in animals, especially during the period of diminished functions.
Conform invenţiei, procedeul include administrarea în calitate de aditiv alimentar a metaboliţilor biomasei tulpinii de Streptomyces fradiae CNMN-Ac-11, în doză de 250 mg/kg masă corporală pe zi, timp de 90 zile până la formarea şi în perioada de formare a reflexelor condiţionate. According to the invention, the process includes the administration as a food additive of metabolites of the biomass of the Streptomyces fradiae strain CNMN-Ac-11, at a dose of 250 mg/kg body weight per day, for 90 days until the formation and during the formation period of conditioned reflexes.
Tulpina este eliminată din solurile Moldovei şi depozitată în Colecţia Naţională a Microorganismelor Nepatogene a AŞM. The strain is removed from Moldovan soils and stored in the National Collection of Nonpathogenic Microorganisms of the ASM.
Analiza compoziţiei biomasei tulpinii Streptomyces fradiae CNMN-Ac-11 denotă că conţinutul aminoacizilor şi al lipidelor diferă de biomasa tulpinii Streptomyces messasporeus CNMN-Ac-36 (soluţia apropiată). Astfel, conţinutul acizilor glutamic şi aspartic, glicinei, prolinei, care îndeplinesc funcţia de neuromediatori şi neuromodulatori în diferite structuri cerebrale şi participă în mecanismele neuroplasticităţii în cazul învăţării, este mai mare decât în biomasa tulpinii Streptomyces messasporeus CNMN-Ac-36. În special conţinutul acidului glutamic este mai sporit cu 126%, al prolinei cu 83%, al glicinei cu 24,8%, iar al acidului aspartic cu 8,4% (Гараева С.Н., Редкозубова Г.В., Постолатий Г.В. Аминокислоты в живом организме. Кишинев, Издательство АНМ, 2009, 552 с). Totodată, în biomasa tulpinii Streptomyces fradiae CNMN-Ac-11 este mai mare conţinutul fosfolipidelor şi sterinelor, iar conţinutul trigliceridelor este mai mic comparativ cu biomasa tulpinii Streptomyces messasporeus CNMN-Ac-36 (Постолакий О.М., Братухина А.А., Бурцева С.А. Липидный состав биомассы стрептомицетов после воздействия электромагнитного излучения миллиметрового диапазона низкой интенсивности. Электронная обработка материалов. 2015, т. 51, № 4, c. 84-89). Luând în considerare că trigliceridele au, în general, un rol energetic, iar fosfolipidele şi sterinele asigură plasticitatea membranei celulelor nervoase, manifestă proprietăţi antioxidante, datorită cărora scade expresivitatea reacţiilor de peroxidare a lipidelor, în stare de stres acut, utilizarea biomasei tulpinii Streptomyces fradiae CNMN-Ac-11 permite de a majora numărul de reacţii condiţionat-reflectoare, reduce numărul de combinaţii ale excitanţilor necondiţionaţi şi condiţionaţi pentru atingerea nivelului de 100% de formare a reflexelor condiţionate. The analysis of the composition of the biomass of the Streptomyces fradiae strain CNMN-Ac-11 shows that the content of amino acids and lipids differs from the biomass of the Streptomyces messasporeus strain CNMN-Ac-36 (the closest solution). Thus, the content of glutamic and aspartic acids, glycine, proline, which perform the function of neuromediators and neuromodulators in various brain structures and participate in the mechanisms of neuroplasticity in the case of learning, is higher than in the biomass of the Streptomyces messasporeus strain CNMN-Ac-36. In particular, the content of glutamic acid is increased by 126%, proline by 83%, glycine by 24.8%, and aspartic acid by 8.4% (Garaeva S.N., Redkozubova G.V., Postolaty G.V. Amino acids in the living organism. Chisinau, ANM Publishing House, 2009, 552 p.). At the same time, in the biomass of Streptomyces fradiae strain CNMN-Ac-11, the content of phospholipids and sterols is higher, and the content of triglycerides is lower compared to the biomass of Streptomyces messasporeus strain CNMN-Ac-36 (Постолакий О.М., Братухина А.А., Бурцева С.А. Липидный сопад биомассы стрептомицетов после продукция элегратномного размый милиметрового разработа низкой интенстиности. Electronic processing materials. 2015, т. 51, № 4, c. 84-89). Considering that triglycerides generally have an energetic role, and phospholipids and sterols ensure the plasticity of the nerve cell membrane, exhibit antioxidant properties, due to which the expressiveness of lipid peroxidation reactions decreases, in a state of acute stress, the use of the biomass of the Streptomyces fradiae CNMN-Ac-11 strain allows to increase the number of conditioned-reflective reactions, reduces the number of combinations of unconditioned and conditioned excitants to achieve the level of 100% formation of conditioned reflexes.
Investigaţiile experimentale au fost realizate pe şobolani albi linia Wistar, întreţinuţi în codiţii de vivariu. În experienţe a fost aplicată metoda formării reflexului artificial exteroreceptor, şi anume a reacţiei condiţionate de evitare activă a excitaţiei nociceptive. Învăţarea şobolanilor a fost realizată conform metodei de evitare activă dublă în camere „shuttle box” după adaptarea timp de 5 min la condiţiile experimentale (Зарайская И.Ю. Системный анализ оборонительного поведения крыс Вистар при обучении двустороннему активному избеганию. Журнал высшей нервной деятельности. 1995, Т. 45, Вып. 3, с. 472-478). Excitarea electrică cutanată (4 mA, 5 kHz) a fost aplicată peste 5 s după semnalul luminos prin podeaua metalică a camerei, conectată la electrostimulator. Zilnic se efectuau câte 10 combinaţii cu un interval de 40±10 s. Reflex condiţionat se consideră trecerea şobolanului într-un compartiment mai sigur, fără întărirea cu un stimul negativ, adică realizarea unui act comportamental anumit. În baza investigaţiilor efectuate se calculează cota reacţiilor condiţionate reflectoare din numărul total de treceri ale fiecărui animal. Experimental investigations were carried out on white Wistar rats, maintained in vivarium conditions. The method of forming an artificial exteroreceptor reflex, namely the conditioned reaction of active avoidance of nociceptive excitation, was applied in the experiments. The rats were trained according to the method of double active avoidance in “shuttle box” chambers after adaptation for 5 min to the experimental conditions (Зарайская I.Ю. Системный анализ оборонительного поведения крыс Вистар при обучении двустороннему активному игбанию. Журнал высшей невровной деятельность. 1995, Т. 45, Вып. 3, с. 472-478). Cutaneous electrical excitation (4 mA, 5 kHz) was applied 5 s after the light signal through the metal floor of the chamber, connected to the electrostimulator. 10 combinations were performed daily with an interval of 40±10 s. A conditioned reflex is considered to be the passage of the rat into a safer compartment, without reinforcement with a negative stimulus, i.e. the performance of a specific behavioral act. Based on the investigations carried out, the share of conditioned reflex reactions in the total number of passages of each animal is calculated.
Exemplul 1 Example 1
A fost studiată activitatea condiţionat reflectoare a şobolanilor tineri (4…4,5 luni). Investigaţiile au fost realizate pe 16 şobolani masculi linia Wistar. La vârsta de o lună animalele au fost arbitrar împărţite în 2 grupe a câte 8 şobolani: grupa martor şi experimentală. Animalele grupei experimentale au primit odată cu hrana timp de 90 zile un preparat ce conţine metaboliţi ai tulpinii Streptomyces fradiae CNMN-Ac-11 (biomasă uscată BM-11) în doză de 250 mg/kg masă corporală. Preparatul a fost amestecat cu hrana. Grupul martor a fost hrănit cu aceeaşi raţie alimentară şi în acelaşi timp cu animalele experimentale. Doza utilizată considerându-se optimală, dozele mai mici de 150 mg/kg nu au efect, iar cele mai mari de 300 mg/kg nu sunt indicate, deoarece creşterea dozei nu asigură un efect major. După 90 zile, pe parcursul a 15 zile s-au format reflexele condiţionate la animalele grupului martor şi celui experimental. Pe tot parcursul experienţei (15 zile) animalele au primit odată cu hrana preparatul BM-11 în doză de 250 mg/kg. The conditioned reflex activity of young rats (4…4.5 months) was studied. The investigations were carried out on 16 male Wistar rats. At the age of one month, the animals were arbitrarily divided into 2 groups of 8 rats each: the control and experimental groups. The animals of the experimental group received, together with the food for 90 days, a preparation containing metabolites of the Streptomyces fradiae strain CNMN-Ac-11 (dry biomass BM-11) in a dose of 250 mg/kg body weight. The preparation was mixed with the food. The control group was fed with the same food ration and at the same time as the experimental animals. The dose used being considered optimal, doses lower than 150 mg/kg have no effect, and those higher than 300 mg/kg are not indicated, since increasing the dose does not provide a major effect. After 90 days, conditioned reflexes were formed in the animals of the control and experimental groups over a period of 15 days. Throughout the experiment (15 days), the animals received the BM-11 preparation in a dose of 250 mg/kg with their food.
Exemplul 2 Example 2
A fost studiată activitatea condiţionat reflectoare a şobolanilor senili (16…16,5 luni). Investigaţiile au fost realizate pe 16 şobolani masculi linia Wistar. La vârsta de 13 luni animalele au fost arbitrar împărţite în 2 grupe a câte 8 şobolani: grupa martor şi experimentală. Animalele grupei experimentale au primit odată cu hrana timp de 90 zile un preparat ce conţine metaboliţi ai tulpinii Streptomyces fradiae CNMN-Ac-11 (biomasă uscată BM-11) în doză de 250 mg/kg masă corporală. Preparatul a fost amestecat cu hrana. Grupul martor a fost hrănit cu aceeaşi raţie alimentară şi în acelaşi timp cu animalele experimentale. Doza utilizată considerându-se optimală, dozele mai mici de 150 mg/kg nu au efect, iar cele mai mari de 300 mg/kg nu sunt indicate, deoarece creşterea dozei nu asigură un efect major. După 90 zile, pe parcursul a 15 zile s-au format reflexele condiţionate la animalele grupului martor şi celui experimental. Pe tot parcursul experienţei (15 zile) animalele au primit odată cu hrana preparatul BM-11 în doză de 250 mg/kg. The conditioned reflex activity of senile rats (16…16.5 months) was studied. The investigations were carried out on 16 male Wistar rats. At the age of 13 months, the animals were arbitrarily divided into 2 groups of 8 rats each: the control and experimental groups. The animals of the experimental group received, together with the food for 90 days, a preparation containing metabolites of the Streptomyces fradiae strain CNMN-Ac-11 (dry biomass BM-11) in a dose of 250 mg/kg body weight. The preparation was mixed with the food. The control group was fed with the same food ration and at the same time as the experimental animals. The dose used being considered optimal, doses lower than 150 mg/kg have no effect, and those higher than 300 mg/kg are not indicated, since increasing the dose does not provide a major effect. After 90 days, conditioned reflexes were formed in the animals of the control and experimental groups over a period of 15 days. Throughout the experiment (15 days), the animals received the BM-11 preparation in a dose of 250 mg/kg with their food.
Datele obţinute sunt incluse în tabel. The data obtained are included in the table.
Tabel Table
Dinamica formării reflexelor condiţionate la şobolanii albi sub influenţa metaboliţilor biomasei tulpinii Streptomyces fradiae CNMN-Ac-11 (BM-11) Dynamics of conditioned reflex formation in white rats under the influence of metabolites of the biomass of the Streptomyces fradiae strain CNMN-Ac-11 (BM-11)
Zile experimentale Cota reflexelor condiţionate din numărul total de treceri (%) Animale tinere Animale senile martor experiment martor experiment 1 0 0 0 0 2 2,0±0,2 6,0±0,7* 0 0 3 5,0±0,4 27,6±1,6* 2,0±0,16 5,0±0,48 4 12,2±0,8 35,7±2,1* 4,0±0,2 12,5±0,9* 5 38.3±2,0 72,1±2,8* 5,0±0,4 26,8±2,4* 6 48,6±2,2 87,2±3,1* 6,8±0,39 28,3±2,8* 7 62,5±2,5 89,0±3,4* 10,8±1,0 38,1±2,9* 8 75,0±2,8 90,6±3,4* 18,2±1,4 39,7±2,9* 9 80,2±3,3 92,0±3,2* 25,7±1,8 52,3±3,1* 10 84,4±3,3 100,0±3,8* 32,6±1,8 65,9±3,5* 11 90,6±3,7 100,0±3,7* 46,3±2,0 72,7±3,8* 12 94,0±3,6 100,0±3,9 48,4±2,2 68,9±3,4* 13 97,4±3,8 100,0±3,6 50,7±2,1 74,4±3,8* 14 100,0±3,8 100,0±3,6 54,7±2,4 75,1±3,9* 15 100,0±3,9 100,0±3,9 60,4±2,4 82,8±3,9*Experimental days Share of conditioned reflexes from the total number of passages (%) Young animals Senile animals control experiment control experiment 1 0 0 0 0 2 2.0±0.2 6.0±0.7* 0 0 3 5.0±0.4 27.6±1.6* 2.0±0.16 5.0±0.48 4 12.2±0.8 35.7±2.1* 4.0±0.2 12.5±0.9* 5 38.3±2.0 72.1±2.8* 5.0±0.4 26.8±2.4* 6 48.6±2.2 87.2±3.1* 6.8±0.39 28.3±2.8* 7 62.5±2.5 89.0±3.4* 10.8±1.0 38.1±2.9* 8 75.0±2.8 90.6±3.4* 18.2±1.4 39.7±2.9* 9 80.2±3.3 92.0±3.2* 25.7±1.8 52.3±3.1* 10 84.4±3.3 100.0±3.8* 32.6±1.8 65.9±3.5* 11 90.6±3.7 100.0±3.7* 46.3±2.0 72.7±3.8* 12 94.0±3.6 100.0±3.9 48.4±2.2 68.9±3.4* 13 97.4±3.8 100.0±3.6 50.7±2.1 74.4±3.8* 14 100.0±3.8 100.0±3.6 54.7±2.4 75.1±3.9* 15 100.0±3.9 100.0±3.9 60.4±2.4 82.8±3.9*
În urma investigaţiilor realizate s-a constatat că la şobolanii care au primit odată cu hrana standardă preparatul ce conţinea metaboliţi ai tulpinii Streptomyces fradiae CNMN-Ac-11, numărul reacţiilor condiţionat-reflectoare era mult mai mare, comparativ cu animalele grupelor de control. Astfel, în prima grupă experimentală cota reflexelor condiţionate din numărul total de treceri, exprimată în procente, este veridic mai mare la animalele grupei experimentale de la a 2-a până la a 11-a zi, iar în grupa a doua experimentală - de la a 2-a zi şi până la sfârşitul experienţei. As a result of the investigations carried out, it was found that in rats that received the preparation containing metabolites of the Streptomyces fradiae strain CNMN-Ac-11 along with the standard food, the number of conditioned reflex reactions was much higher, compared to the animals of the control groups. Thus, in the first experimental group, the share of conditioned reflexes in the total number of passages, expressed in percentage, is significantly higher in the animals of the experimental group from the 2nd to the 11th day, and in the second experimental group - from the 2nd day until the end of the experiment.
Deci, utilizarea biomasei Streptomyces fradiae CNMN-Ac-11 reduce veridic numărul combinaţiilor excitanţilor condiţionaţi şi necondiţionaţi aplicaţi pentru asigurarea nivelului de formare de 100% a reflexelor condiţionate. Analiza rezultatelor obţinute la animalele tinere şi senile demonstrează că la animalele senile acţiunea preparatului BM-11 este mai exprimată, ceea ce denotă despre mecanismul neuroprotector al efectului stimulator al metaboliţilor biomasei Sreptomyces fradiae CNMN-Ac-11 în formarea reflexelor condiţionate. Prin urmare, preparatul BM-11 stimulează formarea reflexelor condiţionate într-o măsură mai mare la animalele senile, decât la animalele tinere, ceea ce permite de a utiliza acest preparat în perioada diminuării funcţiilor. Thus, the use of Streptomyces fradiae CNMN-Ac-11 biomass significantly reduces the number of combinations of conditioned and unconditioned excitants applied to ensure 100% formation of conditioned reflexes. Analysis of the results obtained in young and senile animals demonstrates that in senile animals the action of the BM-11 preparation is more pronounced, which indicates the neuroprotective mechanism of the stimulating effect of the metabolites of Streptomyces fradiae CNMN-Ac-11 biomass on the formation of conditioned reflexes. Therefore, the BM-11 preparation stimulates the formation of conditioned reflexes to a greater extent in senile animals than in young animals, which allows using this preparation during the period of functional decline.
Procedeul propus asigură, comparativ cu cea mai apropiată soluţie, formarea mai rapidă a reflexelor condiţionate, creşterea efectului de stimulare a activităţii condiţionat-reflectoare la animalele experimentale, îndeosebi la cele senile. The proposed procedure ensures, compared to the closest solution, faster formation of conditioned reflexes, increasing the stimulating effect of conditioned-reflective activity in experimental animals, especially in senile ones.
1. Shin-Ya K. et al. Novel neuronal cell protecting substances, aestivophoenins A and B, produced by Streptomyces purpeofuscus. In: J. Antibiotics, 1995, vol. 48, is. 11, р. 1378-1381 1. Shin-Ya K. et al. Novel neuronal cell protecting substances, aestivophoenins A and B, produced by Streptomyces purpeofuscus. In: J. Antibiotics, 1995, vol. 48, Is. 11, p. 1378-1381
2. Kim W.G. et al. Benzastatins H and I, new benzastatin derivatives with neuronal cell protecting activity from Streptomyces nitrosporeus. In: J. Antibiotics, 2001, vol. 54, р. 513-516 2. Kim W.G. et al. Benzastatins H and I, new benzastatin derivatives with neuronal cell protecting activity from Streptomyces nitrosporeus. In: J. Antibiotics, 2001, vol. 54, p. 513-516
3. Shin-Ya K. et al. A novel neuronal cell protecting substance mescengricin produced by Streptomyces griseoflavus. In: J. Asian Natural Products Research, 2000, vol. 2, p. 121-132 3. Shin-Ya K. et al. A novel neuronal cell protecting substance mescengricin produced by Streptomyces griseoflavus. In: J. Asian Natural Products Research, 2000, vol. 2, p. 121-132
4. Шептицкий В.А., Братухина А.А., Бурцева С.А. Условнорефлекторная деятельность белых крыс при длительном потреблении биопрепаратов на основе метаболитов Streptomyces massasporeus. In: Buletinul AŞM. Ştiinţele vieţii, 2007, nr. 2 (302), p. 7-12 4. Шептицкий В.А., Братухина А.А., Burtseva С.А. Conditional reflex activity of white rats during prolonged consumption of biopreparations based on Streptomyces massasporeus metabolites. In: AŞM Bulletin. Life Sciences, 2007, no. 2 (302), p. 7-12
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1069631A3 (en) * | 1980-07-02 | 1984-01-23 | Эли Лилли Энд Компани (Фирма) | Method for preparing tilactone and strain streptomyces fradiae nrrl 12188 for use in preparing tilactone |
| SU1645300A1 (en) * | 1988-12-06 | 1991-04-30 | Научно-Исследовательский Конструкторско-Технологический Институт Биологически Активных Веществ | Streptomyces fradiae strain: producer of restrictase sfr 1 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1069631A3 (en) * | 1980-07-02 | 1984-01-23 | Эли Лилли Энд Компани (Фирма) | Method for preparing tilactone and strain streptomyces fradiae nrrl 12188 for use in preparing tilactone |
| SU1645300A1 (en) * | 1988-12-06 | 1991-04-30 | Научно-Исследовательский Конструкторско-Технологический Институт Биологически Активных Веществ | Streptomyces fradiae strain: producer of restrictase sfr 1 |
Non-Patent Citations (4)
| Title |
|---|
| Kim W.G. et al. Benzastatins H and I, new benzastatin derivatives with neuronal cell protecting activity from Streptomyces nitrosporeus. In: J. Antibiotics, 2001, vol. 54, р. 513-516 * |
| Shin-Ya K. et al. A novel neuronal cell protecting substance mescengricin produced by Streptomyces griseoflavus. In: J. Asian Natural Products Research, 2000, vol. 2, p. 121-132 * |
| Shin-Ya K. et al. Novel neuronal cell protecting substances, aestivophoenins A and B, produced by Streptomyces purpeofuscus. In: J. Antibiotics, 1995, vol. 48, is. 11, р. 1378-1381 * |
| Шептицкий В.А., Братухина А.А., Бурцева С.А. Условнорефлекторная деятельность белых крыс при длительном потреблении биопрепаратов на основе метаболитов Streptomyces massasporeus. In: Buletinul AŞM. Ştiinţele vieţii, 2007, nr. 2 (302), p. 7-12 * |
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