MD1008G2 - Procedeu de obţinere a ficocianinei - Google Patents
Procedeu de obţinere a ficocianineiInfo
- Publication number
- MD1008G2 MD1008G2 MD97-0120A MD970120A MD1008G2 MD 1008 G2 MD1008 G2 MD 1008G2 MD 970120 A MD970120 A MD 970120A MD 1008 G2 MD1008 G2 MD 1008G2
- Authority
- MD
- Moldova
- Prior art keywords
- phycocyanine
- fraction
- less
- invention consists
- biomass
- Prior art date
Links
- 239000002028 Biomass Substances 0.000 abstract 2
- 229920002684 Sepharose Polymers 0.000 abstract 1
- 239000007900 aqueous suspension Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000005194 fractionation Methods 0.000 abstract 1
- 230000002209 hydrophobic effect Effects 0.000 abstract 1
- 230000001376 precipitating effect Effects 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Landscapes
- Cosmetics (AREA)
- Peptides Or Proteins (AREA)
Abstract
Invenţia se referă la biotehnologie şi poate fi utilizată în medicină pentru imunodiagnostic, precum şi în industria alimentară, cosmetică etc.Esenţa invenţiei constă în prepararea suspensiei apoase a biomasei de cianobacterii, congelarea-decongelarea ei în două repetări, separarea biomasei şi fracţionarea consecutivă prin eliminarea fracţiei I din supernatant la pH-ul mai mic sau egal cu 4,0 şi a fracţiei II din precipitat la pH-ul mai mic de punctul izoelectric al proteinelor precipitate. După aceasta pH-ul fracţiilor obţinute se aduce până la 7,5, se efectuează cromatografierea hidrofobă pe fenil-sefaroză şi liofilizarea ficocianinei obţinute.Rezultatul tehnic al invenţiei constă în majorarea gradului de puritate a ficocianinei.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD97-0120A MD1008G2 (ro) | 1997-04-30 | 1997-04-30 | Procedeu de obţinere a ficocianinei |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD97-0120A MD1008G2 (ro) | 1997-04-30 | 1997-04-30 | Procedeu de obţinere a ficocianinei |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MD1008G2 true MD1008G2 (ro) | 1999-06-30 |
Family
ID=62129531
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD97-0120A MD1008G2 (ro) | 1997-04-30 | 1997-04-30 | Procedeu de obţinere a ficocianinei |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1008G2 (ro) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD3781G2 (ro) * | 2008-03-18 | 2009-07-31 | Государственный Университет Молд0 | Procedeu de obţinere a preparatului antioxidant termostabil din biomasa cianobacteriei Spirulina platensis |
| MD4191C1 (ro) * | 2011-12-14 | 2013-07-31 | Государственный Университет Молд0 | Procedeu de extragere a ficocianinei din biomasa algei Spirulina platensis |
-
1997
- 1997-04-30 MD MD97-0120A patent/MD1008G2/ro unknown
Non-Patent Citations (4)
| Title |
|---|
| Archives of Microbiology, v. 120, ą2, 1979, New-York, S. Boussiba, A. Richmond, "Izolation and Characterization of Phycocyanin from the Blue-Green Alga Spirulina platensis", p. 155-159. * |
| Archives of Microbiology, v. 128, ą1, 1980, New-York, V.S. Tyagi, B.C. Mayne, G.A. Peters, "Purification and Initial Characterization of Phycobiliproteins from the Endophytic Cyanobacterium of Azolae", p. 41-44. * |
| The Biochemical Journal, v.106, ą2, 1968, Great Britain, F.W.Craig, N.G. Carr, "C-Phycocyanin and Allophycocyanin in Tow Species of Blue-Green Algae", p. 361-367. * |
| Изв. АН Молд. ССР, Серия биологических и химических наук, №4, 1987, "Штиинца", Кишинев, И.Вимер, И.Вайнтрауб, "Фикобилипротеины из синезеленых водорослей", с. 20-23. * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD3781G2 (ro) * | 2008-03-18 | 2009-07-31 | Государственный Университет Молд0 | Procedeu de obţinere a preparatului antioxidant termostabil din biomasa cianobacteriei Spirulina platensis |
| MD4191C1 (ro) * | 2011-12-14 | 2013-07-31 | Государственный Университет Молд0 | Procedeu de extragere a ficocianinei din biomasa algei Spirulina platensis |
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