MA39079A1 - Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus - Google Patents

Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus

Info

Publication number
MA39079A1
MA39079A1 MA39079A MA39079A MA39079A1 MA 39079 A1 MA39079 A1 MA 39079A1 MA 39079 A MA39079 A MA 39079A MA 39079 A MA39079 A MA 39079A MA 39079 A1 MA39079 A1 MA 39079A1
Authority
MA
Morocco
Prior art keywords
collagen
acid
scales
sardine
native
Prior art date
Application number
MA39079A
Other languages
French (fr)
Other versions
MA39079B1 (en
Inventor
Belali Fatima
Tenzali Zakaria
Radi Youssef
Alhayane Hassan
Hanoun Said
Chaira Jamal
Akesbi Abdelilah
Laaziouz Issam
Original Assignee
Inrh
Haliopole Cluster Agadir
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inrh, Haliopole Cluster Agadir filed Critical Inrh
Priority to MA39079A priority Critical patent/MA39079B1/en
Priority to PCT/MA2017/000013 priority patent/WO2017209587A1/en
Publication of MA39079A1 publication Critical patent/MA39079A1/en
Publication of MA39079B1 publication Critical patent/MA39079B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]

Abstract

La présente invention concerne un procédé d'extraction acide à faible température et depurification de collagène marin natif type 1 soluble à partir des écailles de sardine (sardinepilchardus) fraiches ou congelées, caractérisée en ce qu'il comprend par les étapes de 1)lavage à l'eau des écailles, 2) séchage des écailles 3) traitement alcalin pour éliminer lesprotéines non collagénique, 4) traitement acide pour éliminer les minéraux, 5)extraction àfroid à ph acide pour solubiliser les fibrilles de collagène natif, 6)ultracentrifugation pouréliminer les gros agrégats, 7) précipitation du collagène par adjonction d'un sel neutre 8)remise en suspension du collagène précipité et 9) dialyse au moyen d'un sac de dialyse. Ceprocédé permet dans un délai raisonnable de produire un collagène avec une puretémaximale en supprimant la quasi-totalité des impuretés non collagénique et des minéraux,en solubilisant la totalité du collagène et en limitant sa dénaturation. En plus de l'obtentionde collagène marin natif type 1avec un rendement considérable, ce procédé optimisé par laméthode de réponse de surfacersm permet de réduire la durée du traitement alcalin etacide, de diminuer considérablement la durée de l'extraction acide. Il permet égalementd'amoindrir la quantité de réactifs utilisés en l'occurrence, la soude, l'acidedéminéralisateur et l'acide extracteur. Le procédé résout le problème particulier de lagestion des produits chimiques, de manière plus durable et plus économique. Le collagènenatif de haute qualité ainsi obtenu est propice à des applications en alimentaire, encosmetfood et en biomédical.The present invention relates to a process for low temperature acid extraction and purification of soluble native marine collagen type 1 from sardine scales (sardinepilchardus) fresh or frozen, characterized in that it comprises by the steps of 1) washing at scaling water, 2) drying of scales 3) alkaline treatment to remove non-collagenic proteins, 4) acid treatment to remove minerals, 5) acid ph-cold extraction to solubilize native collagen fibrils, 6) ultracentrifugation to eliminate aggregates, 7) collagen precipitation by addition of a neutral salt 8) resuspension of precipitated collagen and 9) dialysis by means of a dialysis bag. This method allows within a reasonable time to produce a collagen with a pureteamximal eliminating almost all non-collagenic impurities and minerals, by solubilizing all the collagen and limiting its denaturation. In addition to obtaining a type 1 native marine collagen with a considerable yield, this method optimized by the surfacersm response method makes it possible to reduce the duration of the alkaline and acid treatment, considerably reducing the duration of the acid extraction. It also reduces the amount of reagents used in this case, sodium hydroxide, acemineminaliser and extractor acid. The process solves the particular problem of managing chemicals in a more sustainable and economical way. The resulting high quality collagen is suitable for food, encosmetfood and biomedical applications.

MA39079A 2016-06-01 2016-06-01 Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus MA39079B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MA39079A MA39079B1 (en) 2016-06-01 2016-06-01 Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus
PCT/MA2017/000013 WO2017209587A1 (en) 2016-06-01 2017-06-01 Method for the extraction and purification of native marine collagen from sardina pilchardus sardine scales

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MA39079A MA39079B1 (en) 2016-06-01 2016-06-01 Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus

Publications (2)

Publication Number Publication Date
MA39079A1 true MA39079A1 (en) 2018-01-31
MA39079B1 MA39079B1 (en) 2018-04-30

Family

ID=59626651

Family Applications (1)

Application Number Title Priority Date Filing Date
MA39079A MA39079B1 (en) 2016-06-01 2016-06-01 Process for extracting and purifying native marine collagen type I from sardine scales sardina pilchardus

Country Status (2)

Country Link
MA (1) MA39079B1 (en)
WO (1) WO2017209587A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3108116B1 (en) * 2020-03-11 2022-11-25 Institut National De Rech Pour L’Agriculture L’Alimentation Et L’Environnement Process for the manufacture of compounds of interest from at least one mineralized connective tissue, and compounds of interest resulting from this manufacturing process
CN111743107A (en) * 2020-07-14 2020-10-09 黄山市胡兴堂桃花流水食品有限公司 Flavor-enhanced rapid fermentation method for smelly mandarin fish
CN112352831A (en) * 2020-11-13 2021-02-12 泉州师范学院 Edible antibacterial composite membrane liquid based on waste fish scales as well as preparation method and application thereof
CN112574293B (en) * 2020-12-11 2022-12-16 浙江万里学院 Process for decalcifying and extracting collagen from turtle shell by using ultrasonic assistance
CN112680494A (en) * 2021-02-04 2021-04-20 海南华研胶原科技股份有限公司 Method for extracting collagen oligopeptide from deep-sea fish skin

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102321719A (en) 2011-09-28 2012-01-18 山东天久生物技术有限公司 Industrial production method for preparing collagen from fish scale by enzyme method
CN102586373A (en) 2012-01-16 2012-07-18 郁小兵 Method for extracting high-quality collagen at low temperature
CN102839207A (en) 2012-09-17 2012-12-26 温州大学 Method for preparing collagen peptide from fish scales

Also Published As

Publication number Publication date
WO2017209587A1 (en) 2017-12-07
MA39079B1 (en) 2018-04-30

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