CN112680494A - Method for extracting collagen oligopeptide from deep-sea fish skin - Google Patents
Method for extracting collagen oligopeptide from deep-sea fish skin Download PDFInfo
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- CN112680494A CN112680494A CN202110155531.8A CN202110155531A CN112680494A CN 112680494 A CN112680494 A CN 112680494A CN 202110155531 A CN202110155531 A CN 202110155531A CN 112680494 A CN112680494 A CN 112680494A
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- deep
- fish skin
- collagen oligopeptide
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- sea fish
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 43
- 102000008186 Collagen Human genes 0.000 title claims abstract description 37
- 108010035532 Collagen Proteins 0.000 title claims abstract description 37
- 229920001436 collagen Polymers 0.000 title claims abstract description 37
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 25
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 40
- 238000002791 soaking Methods 0.000 claims abstract description 35
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 26
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims abstract description 18
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims abstract description 13
- 239000000920 calcium hydroxide Substances 0.000 claims abstract description 13
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 235000017557 sodium bicarbonate Nutrition 0.000 claims abstract description 9
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims abstract description 9
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 108090000526 Papain Proteins 0.000 claims abstract description 6
- 229940055729 papain Drugs 0.000 claims abstract description 6
- 235000019834 papain Nutrition 0.000 claims abstract description 6
- 238000004332 deodorization Methods 0.000 claims abstract description 5
- 238000005406 washing Methods 0.000 claims abstract description 5
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 24
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 12
- 239000001103 potassium chloride Substances 0.000 claims description 12
- 235000011164 potassium chloride Nutrition 0.000 claims description 12
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 9
- 238000002360 preparation method Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 108091005658 Basic proteases Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 108050001049 Extracellular proteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Abstract
The invention discloses a method for extracting collagen oligopeptide from deep-sea fish skin, which comprises the following steps: the first step is as follows: product treatment, namely peeling the deep sea fish to obtain raw material fish skin, and then soaking the raw material fish skin in baking soda for deodorization treatment; the second step is that: treating the raw material with pH, soaking with hydrochloric acid, stirring intermittently to pH 6-7, soaking with calcium hydroxide, stirring intermittently, and washing with ionized water to pH medium. According to the method, the collagen peptide can be better extracted from the skin of the deep-sea fish through the papain, and meanwhile, the preparation process is stable, so that the collagen peptide product can be effectively produced.
Description
Technical Field
The invention belongs to the technical field of deep sea fish skin extraction, and particularly relates to a method for extracting collagen oligopeptide from deep sea fish skin.
Background
The deep-sea fish skin collagen is collagen extracted from deep-sea fish skin as a raw material as well as the name suggests. Due to the low temperature and low pressure of deep sea, the collagen has special properties which are not possessed by common terrestrial animal collagen and collagen of freshwater fish and shallow sea fish.
The collagen peptide is an extracellular protein, consists of two or more amino acids to form protein peptide, and is absorbed by human body in a peptide mode, so that the absorption utilization rate of the edible protein peptide can reach 100%. Collagen is the most important component of the extracellular matrix.
However, in the preparation process, the enzymolysis effect of the commonly used medium-sized protease, alkaline protease and trypsin is not optimal, so that the preparation effect of extracting the collagen peptide is influenced.
Disclosure of Invention
The invention aims to provide a cement ball mill convenient for discharging, which aims to solve the problem of poor enzymolysis effect of common protease.
In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting collagen oligopeptide from deep-sea fish skin comprises the following steps:
the first step is as follows: product treatment, namely peeling the deep sea fish to obtain raw material fish skin, and then soaking the raw material fish skin in baking soda for deodorization treatment;
the second step is that: treating the pH value of the raw material, soaking the raw material by hydrochloric acid, intermittently stirring the raw material until the pH value is between 6 and 7, soaking the raw material by calcium hydroxide, intermittently stirring the raw material, and cleaning the raw material by using ionized water until the pH value is medium;
the third step: pretreating before processing, soaking with sodium hydroxide solution until fish skin is transparent, washing with ionized water until pH is 8, cutting, and homogenizing;
the fourth step: putting the pretreated raw materials into boiling water according to a certain material-liquid ratio, heating, cooling after a certain time, and then adding papain;
the fifth step: preparing active carbon and potassium chloride, waiting for enzymolysis reaction, deactivating enzyme after the enzymolysis reaction is finished, sequentially adding the active carbon and the potassium chloride, and finally layering, purifying and drying standing liquid to obtain the product.
Preferably, the concentration of the baking soda in the first step is 6 to 8mol/L, and the soaking time is 60 minutes.
Preferably, the hydrochloric acid concentration in the second step is 1.2%, and the calcium hydroxide concentration is 0.04%.
Preferably, the hydrochloric acid soaking time and the calcium hydroxide soaking time in the second step are both 2 to 3 days, and the solution is changed every 18 hours.
Preferably, the concentration of the sodium hydroxide solution in the third step is 6.5%, and the soaking time is 20 minutes.
Preferably, the ratio of the material to the liquid in the fourth step is 1.2: 29, the heating time is half an hour, and the cooling temperature is not more than 40 ℃.
Preferably, the enzymolysis pH in the fifth step is 7.3 to 9, the enzymolysis temperature is 35 ℃, and the enzymolysis time is 6 to 8 hours.
Preferably, the concentration of activated carbon in the fifth step is 2 to 3 mg/mL.
Preferably, the concentration of potassium chloride in the fifth step is 1.5 to 3.5 mg/mL.
Compared with the prior art, the invention has the beneficial effects that: according to the method, the collagen peptide can be better extracted from the skin of the deep-sea fish through the papain, and meanwhile, the preparation process is stable, so that the collagen peptide product can be effectively produced.
Detailed Description
Example 1
A method for extracting collagen oligopeptide from deep sea fish skin comprises the following steps:
the first step is as follows: product treatment, namely peeling the deep sea fish to obtain raw material fish skin, and then soaking the raw material fish skin in baking soda for deodorization treatment;
the second step is that: treating the pH value of the raw material, soaking the raw material by hydrochloric acid, intermittently stirring the raw material until the pH value is between 6 and 7, soaking the raw material by calcium hydroxide, intermittently stirring the raw material, and cleaning the raw material by using ionized water until the pH value is medium;
the third step: pretreating before processing, soaking with sodium hydroxide solution until fish skin is transparent, washing with ionized water until pH is 8, cutting, and homogenizing;
the fourth step: putting the pretreated raw materials into boiling water according to a certain material-liquid ratio, heating, cooling after a certain time, and then adding papain;
the fifth step: preparing active carbon and potassium chloride, waiting for enzymolysis reaction, deactivating enzyme after the enzymolysis reaction is finished, sequentially adding the active carbon and the potassium chloride, and finally layering, purifying and drying standing liquid to obtain the product.
The concentration of the baking soda in the first step is 8mol/L, and the soaking time is 60 minutes.
In the second step, the concentration of hydrochloric acid was 1.2% and the concentration of calcium hydroxide was 0.04%.
The hydrochloric acid soaking time and the calcium hydroxide soaking time in the second step are both 2 days, and the solution is changed every 18 hours.
The concentration of the sodium hydroxide solution in the third step is 6.5%, and the soaking time is 20 minutes.
The material-liquid ratio in the fourth step is 1.2: 29, the heating time is half an hour, and the cooling temperature is not more than 40 ℃.
The enzymolysis PH in the fifth step is 9, the enzymolysis temperature is 35 ℃, and the enzymolysis time is 6 hours
The activated carbon concentration in the fifth step was 3 mg/mL.
The potassium chloride concentration in the fifth step was 1.5 mg/mL.
Example 2
A method for extracting collagen oligopeptide from deep sea fish skin comprises the following steps:
the first step is as follows: product treatment, namely peeling the deep sea fish to obtain raw material fish skin, and then soaking the raw material fish skin in baking soda for deodorization treatment;
the second step is that: treating the pH value of the raw material, soaking the raw material by hydrochloric acid, intermittently stirring the raw material until the pH value is between 6 and 7, soaking the raw material by calcium hydroxide, intermittently stirring the raw material, and cleaning the raw material by using ionized water until the pH value is medium;
the third step: pretreating before processing, soaking with sodium hydroxide solution until fish skin is transparent, washing with ionized water until pH is 8, cutting, and homogenizing;
the fourth step: putting the pretreated raw materials into boiling water according to a certain material-liquid ratio, heating, cooling after a certain time, and then adding papain;
the fifth step: preparing active carbon and potassium chloride, waiting for enzymolysis reaction, deactivating enzyme after the enzymolysis reaction is finished, sequentially adding the active carbon and the potassium chloride, and finally layering, purifying and drying standing liquid to obtain the product.
The concentration of the baking soda in the first step is 6mol/L, and the soaking time is 60 minutes.
In the second step, the concentration of hydrochloric acid was 1.2% and the concentration of calcium hydroxide was 0.04%.
The hydrochloric acid soaking time and the calcium hydroxide soaking time in the second step are both 3 days, and the solution is changed every 18 hours.
The concentration of the sodium hydroxide solution in the third step is 6.5%, and the soaking time is 20 minutes.
The material-liquid ratio in the fourth step is 1.2: 29, the heating time is half an hour, and the cooling temperature is not more than 40 ℃.
The enzymolysis PH in the fifth step is 9, the enzymolysis temperature is 35 ℃, and the enzymolysis time is 8 hours
The activated carbon concentration in the fifth step was 2 mg/mL.
The potassium chloride concentration in the fifth step was 3.5 mg/mL.
Claims (9)
1. A method for extracting collagen oligopeptide from deep-sea fish skin is characterized by comprising the following steps: the method for extracting the collagen oligopeptide from the deep-sea fish skin comprises the following steps:
the first step is as follows: product treatment, namely peeling the deep sea fish to obtain raw material fish skin, and then soaking the raw material fish skin in baking soda for deodorization treatment;
the second step is that: treating the pH value of the raw material, soaking the raw material by hydrochloric acid, intermittently stirring the raw material until the pH value is between 6 and 7, soaking the raw material by calcium hydroxide, intermittently stirring the raw material, and cleaning the raw material by using ionized water until the pH value is medium;
the third step: pretreating before processing, soaking with sodium hydroxide solution until fish skin is transparent, washing with ionized water until pH is 8, cutting, and homogenizing;
the fourth step: putting the pretreated raw materials into boiling water according to a certain material-liquid ratio, heating, cooling after a certain time, and then adding papain;
the fifth step: preparing active carbon and potassium chloride, waiting for enzymolysis reaction, deactivating enzyme after the enzymolysis reaction is finished, sequentially adding the active carbon and the potassium chloride, and finally layering, purifying and drying standing liquid to obtain the product.
2. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the concentration of the baking soda in the first step is 6-8 mol/L, and the soaking time is 60 minutes.
3. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the concentration of hydrochloric acid in the second step is 1.2%, and the concentration of calcium hydroxide is 0.04%.
4. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the hydrochloric acid soaking time and the calcium hydroxide soaking time in the second step are both 2 to 3 days, and the solution is changed every 18 hours.
5. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the concentration of the sodium hydroxide solution in the third step is 6.5%, and the soaking time is 20 minutes.
6. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the material-liquid ratio in the fourth step is 1.2: 29, the heating time is half an hour, and the cooling temperature is not more than 40 ℃.
7. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: and the pH value of enzymolysis in the fifth step is 7.3-9, the enzymolysis temperature is 35 ℃, and the enzymolysis time is 6-8 hours.
8. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the concentration of the activated carbon in the fifth step is 2 to 3 mg/mL.
9. The method for extracting collagen oligopeptide from deep-sea fish skin according to claim 1, wherein the collagen oligopeptide comprises the following components: the concentration of potassium chloride in the fifth step is 1.5 to 3.5 mg/mL.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110155531.8A CN112680494A (en) | 2021-02-04 | 2021-02-04 | Method for extracting collagen oligopeptide from deep-sea fish skin |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110155531.8A CN112680494A (en) | 2021-02-04 | 2021-02-04 | Method for extracting collagen oligopeptide from deep-sea fish skin |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382806A (en) * | 2002-05-15 | 2002-12-04 | 中国科学院上海有机化学研究所 | Collagen used for deep-sea fish skin tissue engineering and its preparing process |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| WO2017209587A1 (en) * | 2016-06-01 | 2017-12-07 | Institut National De Recherche Halieutique | Method for the extraction and purification of native marine collagen from sardina pilchardus sardine scales |
| CN112062834A (en) * | 2020-11-16 | 2020-12-11 | 江西中医药大学 | Deep sea fish skin collagen peptide and extraction and preparation method thereof |
-
2021
- 2021-02-04 CN CN202110155531.8A patent/CN112680494A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1382806A (en) * | 2002-05-15 | 2002-12-04 | 中国科学院上海有机化学研究所 | Collagen used for deep-sea fish skin tissue engineering and its preparing process |
| CN102559826A (en) * | 2012-02-15 | 2012-07-11 | 胡如桂 | Method for preparing collagen oligopeptide |
| WO2017209587A1 (en) * | 2016-06-01 | 2017-12-07 | Institut National De Recherche Halieutique | Method for the extraction and purification of native marine collagen from sardina pilchardus sardine scales |
| CN112062834A (en) * | 2020-11-16 | 2020-12-11 | 江西中医药大学 | Deep sea fish skin collagen peptide and extraction and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 李玉环主编: "《水产品加工技术》", 30 September 2014 * |
| 顾斌洲等: "鳕鱼皮制备胶原蛋白肽的工艺优化", 《浙江海洋学院学报(自然科学版)》 * |
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Application publication date: 20210420 |