MA38729B1 - Kit de diagnostic de la tuberculose - Google Patents

Kit de diagnostic de la tuberculose

Info

Publication number
MA38729B1
MA38729B1 MA38729A MA38729A MA38729B1 MA 38729 B1 MA38729 B1 MA 38729B1 MA 38729 A MA38729 A MA 38729A MA 38729 A MA38729 A MA 38729A MA 38729 B1 MA38729 B1 MA 38729B1
Authority
MA
Morocco
Prior art keywords
present
tuberculosis
amplification technique
diagnostic kit
lamp
Prior art date
Application number
MA38729A
Other languages
English (en)
Inventor
El Mehdi Bentaleb
Benhassou Hassan Ait
El Hassane Sefrioui
Original Assignee
Mascir Morrocan Foundation For Advanced Science Innovation & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mascir Morrocan Foundation For Advanced Science Innovation & Res filed Critical Mascir Morrocan Foundation For Advanced Science Innovation & Res
Priority to MA38729A priority Critical patent/MA38729B1/fr
Priority to PCT/MA2016/000032 priority patent/WO2017116220A1/fr
Publication of MA38729B1 publication Critical patent/MA38729B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé et une trousse pour le diagnostic in vitro de la tuberculose chez un sujet. Le procédé en question permet d'asseoir un diagnostic direct rapide, fiable en utilisant une technique d'amplification des acides nucléiques, spécifiquement la technique d'amplification isotherme induite par boucle (lamp) améliorée sans étape de dénaturation initiale et sans étape d'inactivation enzymatique finale. En accordance avec la présente invention, la présente méthode améliorée de lamp permettra la détection et l'identification directe, d'agents pathogènes causant la tuberculose ou d'autres maladies infectieuses chez l'homme sans ou avec extraction.
MA38729A 2015-12-29 2015-12-29 Kit de diagnostic de la tuberculose MA38729B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MA38729A MA38729B1 (fr) 2015-12-29 2015-12-29 Kit de diagnostic de la tuberculose
PCT/MA2016/000032 WO2017116220A1 (fr) 2015-12-29 2016-12-29 Kit de diagnostic de la tuberculose

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MA38729A MA38729B1 (fr) 2015-12-29 2015-12-29 Kit de diagnostic de la tuberculose

Publications (1)

Publication Number Publication Date
MA38729B1 true MA38729B1 (fr) 2018-04-30

Family

ID=58228514

Family Applications (1)

Application Number Title Priority Date Filing Date
MA38729A MA38729B1 (fr) 2015-12-29 2015-12-29 Kit de diagnostic de la tuberculose

Country Status (2)

Country Link
MA (1) MA38729B1 (fr)
WO (1) WO2017116220A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019130347A1 (fr) * 2017-12-26 2019-07-04 Sree Chitra Tirunal Institute For Medical Sciences And Technology Amorces pour l'amplification isotherme du gène mpt64 de mycobacterium tuberculosis et processus associé
WO2019136034A1 (fr) * 2018-01-02 2019-07-11 California Institute Of Technology Test basé sur une amplification isothermique à médiation par boucle (lamp) pour la détection de microbes
CN112011633A (zh) * 2020-10-14 2020-12-01 河南智泰生物科技有限公司 LAMP同时检测结核杆菌复合菌群及rpoB基因突变的引物组、试剂盒及方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102373273B (zh) * 2010-08-26 2013-07-03 杭州优思达生物技术有限公司 一种结核分枝杆菌核酸的检测试剂盒

Also Published As

Publication number Publication date
WO2017116220A1 (fr) 2017-07-06

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Tubbs The art of procreation and the members employed therein are so repulsive, that if it were not for the beauty of the faces and the adornments of the actors and the pent-up impulse, nature would lose the human species