LV14463B - Cosmetic preparation for inhibiting hair growth - Google Patents

Abstract

There is offered cosmetic preparation for inhibiting hair growth. The obtained composition contains in defined weight ratio Bertholetia Excelsa Nut, Salix Alba Bark Extract, Hamamelis Virginiana Extract, Caprylic/Capric Triglyceride, Allantoin, Urea, Glycerol, Ethoxydiglycol, Menthol, Methylchloroisothiazolinone, Methylisothiazolinone, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer, Cymbopogon Schoenanthus Oil, Butylhydroxytoluene and water. The offered compositions inhibit hair growth as well as perfect appearance of skin and improve consumers’ feeling.

Description

Hair plays an important biological role in mechanically protecting parts of the body. From a cosmetic point of view, scalp hair is different from body hair. Scalp hair is added to

i.e. for terminal hairs that vary in length, diameter and degree of pigmentation. Body hair is divided into two types of transition hair and fluff according to length, thickness and degree of pigmentation. For young people, body hair is classified as fluff. As a person grows older, androgens promote the conversion of fluff into hairs and, in certain parts of the body, to terminal hair and their localization is gender-specific.

It has long been a common belief that from the aesthetic and hygienic point of view hair on the legs, arms, armpits, etc. not desirable. Nowadays, the problem of combating unwanted hair has become a reality for both women and men. These beliefs have led to the rapid development of technologies for removing unwanted hair, using cutting, shaving, creams, pulling, and so on. Most hair removal methods are painful and give different results. Cosmetic treatments inhibit hair growth, make them thinner and less visible. Depilation means removing hair on the surface of the skin without touching the root, while epilation means removing hair on the subcutaneous layer. Shaving or chemical depilation provides a short-term effect. Depilation products often irritate the skin.

The fight against unwanted hair is also topical from a medical point of view. A number of endocrine disorders are known, characterized by increased hair growth on facial women (hirsutism).

Waxing methods include hair removal; electro-epilation (by the principle of hair extraction) is a long-term invasive technique that increases the risk of hyperpigmentation of the skin during frequent use. Electroepilation, photoepilation, laser epilation (destruction of the bulb of the hair) can cause skin pigmentation disorders, erythema, edema (Lolis M.S., Marmur E.S.J. Cosmet. Dermatol. 2006, 5, 4, 274-6),

Using non-invasive methods, it is difficult to simultaneously remove the visible part of the hair and destroying the bulb. The chemicals used cause cytotoxic effects on germinal cells. Enzyme-containing products destroys the bulb, thereby inhibiting hair growth. Hair growth is also inhibited by physiological methods - disrupting the hair growth cycle, which includes 3 phases: anagen - growth phase; catagen - the shortest phase characterized by slower cell division; telogen - the dormant phase, hair no longer grows and falls out.

In practice, none of the above methods of hair growth suppression can provide 100% effectiveness. The solution to the problem is, first of all, to do hair removal with hair removal, which results in the formation of a "pore" leading to the hair bulb. In addition, synthetic and natural substances (alkalizing agents, enzymes, vegetable oil extracts) should provide follicular destroying. In this context, there is the problem of transporting the active components to the follicle.

Research on patent databases has led to the discovery of some thematic patents, of which the patent application WO2008117122 (A2), publ. Date 2008-10-02 Compositions and method for removing hair. This patent application proposes the use of isopropanol and other substances to "deliver" follicle cell enzyme inhibitors (antibiotics, essential compounds, etc.) to target cells.

In patent application JP2009079050 (A), publ. Date 2009-04-16 offers the use of special agents that are activated by electromagnetic radiation or ultrasound after incorporation into the follicle. Flutamide-based composition (chemotherapeutic agent, anti-androgen) is proposed as a medicament - patent application RU2123838 (Cl), published December 27, 1998. WO0180816 (A1), Publ. Date 2001-1 ΙΟΙ, the authors suggest using horseradish peroxidase, which is activated in the follicle after waxing. Incorporation of a photosensitizing agent into the follicles by liposomes and subsequent skin light irradiation has been described (patent application WO9732046 (Al), published 1997-0904). A method based on multiple applications of soy-derived components has also been developed (CA2472745 (Al), published 01-01-2005).

Analyzing the available information, it can be concluded that technology based on the application of depilation and hair growth inhibiting compositions seems to be the most suitable for removing unwanted hair. The greatest activity of researchers is focused on the search for hair growth inhibitors, especially among natural products. Natural substances with the lowest degree of side effects are traditionally widely used for beauty purposes. Non-medical products are better suited for mass use.

Compositions of natural substances used during post-depilation fall into two groups: enzymes and plant extracts. Enzymes are used to inactivate hair follicle structures that lead to inhibition of hair growth. Thus, there is provided a composition comprising an enzyme and a transdermal transport activator (HR 20090407 (Tl), published on 2009-09-30). Another study used a proteolytic enzyme composition from papain, trypsin, chymotrypsin, pepsin, bromelain, etc. (US 200214604 (Al), published 10-10-2002). Enzymes have a pronounced epilating effect but develops in parallel with increasing the thickness of the epidermis, which worsens the appearance of the skin (Traversa E. et al.Int.J.Pharm., 2007,235,1-2,163-6).

The solution to the problem could be achieved by combining enzymes with various plant components that reduce the side effects of the preparation. Hair growth inhibitory compositions consisting of proteolytic enzymes of pineapple and kiwi fruit, as well as aloe, kudu root, chlorella extract, collagen, honey and propolis extracts, etc. are offered (). But as a result, the product ends up being very expensive and less accessible to consumers.

A more promising line of research focuses on the use of plant components. There is a distinction to be made here between plants which are classified as medicinal products (for example, see US 2008103143 (Al), published on 2008-05-01). Japanese authors propose the use of essences derived from white chrysanthemum flowers and burdock roots and leaves to inhibit hair growth (JP 200696750 (A), published April 13, 2006). An even more sophisticated composition is offered in another study: Cork bark, Coptis japonica, Serenoa repens, Hypericum erectus, Impatiens balsamina, Lagerstroemia speciosa extracts and a whole range of synthetically derived substances (JP 2009084255 (A), published 2009-04-23). Most of the transferred plants are exotic and scarce available. The composition of the extracts of Humulus lupulus, Carica papaya, Thuya occidentalis, Ononis spinosa, Cucurbita pepo (WO 2009090213 (A2), published 2009-07-23) has poor efficacy.

The oil-based composition of Urtica dioica, Conium, Foeniculum, Lupinus, Pimpinella should be administered 3 times daily for 3-5 days (WO 2010007553 (A2), published on 2010-01-21), which reduces consumer compliance. In addition, lupine belongs to plants whose safety is questioned (Ressa D. et al. Mol. Nutr. Food Res. 2008,52,4,490-5).

There is provided a composition of the pulse components which is used twice daily but the duration of the course is not disclosed in the patent application (CA 2472745 (Al), published on 01-01-2005). This method also includes pre-exposure of the skin to light.

A "perfect" hair growth retardant could be considered a non-toxic composition that would inhibit long-term hair growth, would not cause skin irritation, allergic reactions, and would be comfortable to use.

Disclosure of the Invention

The purpose of the present invention is to provide a safe, long-lasting hair growth suppressant cosmetic based on natural substances.

A composition that blocks important biochemical processes on the hair bulb cells looks close to the optimal cosmetic. Thus, patent MX 9603929 (A), published April 30, 1997, discloses the use of medicaments (verapamil, thioridazine, trifluoperazine

etc.) as well as a combination of curcumin, a vitamin C derivative, and the protein kinase C enzyme. The effect of this preparation is based on the effects of tank-dependent biochemical processes affecting hair follicles. The active substances are transported to the skin cells using appropriate carriers such as acetone, ethyl alcohol and the like. The said composition is provided twice or more times a day. It should be noted that the inclusion of pharmacological substances in the formulation gives rise to the classification of the product as a medicinal product, which in turn reduces the availability of the product to the consumer.

In the presence of the trace element selenium in microorganisms (Razak A. A. et al., Biol. Trace Elem. Res. 1990, 25, 3, 187-92) and plants (Bumell JN Amer. Soc. Plant. Biol. , 1981, 67, 316-324; Freeman JL et al., Plant Physiol. 2010, 153, 1630-1652), Se and amino acid compounds selenocistern and selenomethionine are formed by replacing the sulfur atom with selenium. As a result, atypical -S-S- bonds are formed between selenium amino acid compounds that are part of the protein structure. These changes lead to impaired function of the relevant protein molecules. Hair bulb cells are rich in sulfur-containing amino acids. Therefore, the cells are sensitive to selenium.

The hypothesis of our experimental research work was related to the idea of using plants rich in selenium to develop a new cosmetic product. There are about 20 such known plants, some of which are toxic, such as Astragalus bisulcatus and Stanleya pinnata (Plant Physiol. 2006,142,1,124-34), while others are edible - Opuntia ficus-indica fruits (Amer. J. Clin. Nutr., 2004, 80,2,391-395), vegetables of the genus Brassica, incl. broccoli. Agro-techniques for increasing the selenium content in broccoli have been developed (US 6958435 B1, Publication Date 2005-25-10).

We consider the Brazilian nut (Bertholettia excelsa) to be the most suitable herb for developing new hair growth inhibitors because it is not only rich in selenium (Plotkin M., Famolare L. Eds. Island Press, 1992, Washington, DC). ), but its oil is used in the manufacture of cosmetics http://ec.europa.eu/consumers/sectors/cosmetics/cosing/ingredients/index en.htm). Brazil nut contains about 67% fat (www.health.24.com).

Bertholettia excelsa has so far not been used for hair growth inhibition in either cosmetic or pharmacological products.

In our experiments we used blanched and dried nuts. The nuts were crushed with the help of rollers and a thread press, the oil was squeezed by the cold method.

Walnut cakes were made into pasta with a particle size of 30-50 mkm. For this purpose we used a disintegrator with 4 rotors of different diameters, which are inserted into each other. Two rotors rotate in one direction, two rotate in opposite direction. The rotor walls have holes of a size that shrinks from the outer rotor to the inner one. The nut cake particles move through the walls of the rotors to the middle part of the structure and gradually grind to the required size.

The paste obtained contained oil in the range of 7% -15%. This paste was used for further research.

Laboratory mice were tested to evaluate Brazilian nut hair growth inhibitory activity. The composition of natural substances prepared was as follows: brazil nut paste, glycerin, emulsifier (see Table 4), water.

Generally made in 5 variants of a Brazil nuts product: with a nut paste content of 0.5, 10.20, or 25 g / 100 g.

Along with changes in the amount of Brazil nuts in the paste, the amount of glycerol added was adjusted; 0-5-10-20 g / 100 g of brazil nut content corresponds to a glycerol content of 22,517,5-7,5-2,5 g / 100 g in the composition; In the case of 25% Brazil nut cream, the glycerol concentration was 2.5 g / 100 g and the water content was reduced to 29.27 g / 100 g. The emulsifier concentration in all variants was 2 g / 100 g.

In the experimental study, laboratory mice (males), 4 weeks old, were randomized to 7 groups (n = 7). The animals were kept under standard vivarium conditions, fed a standard ration. The experiment was approved by the Animal Ethics Board.

All animals except Group 1 (intact) were machine-shaved on the dorsal surface (patch area about 4 cm2; Fig. A).

t- Table. Design of experimental study of Brazil nut.

Group # Group characteristics 1. The intact 2. Feather Shaved (Control) 3. Intermediate product without Brazil nuts 4. Intermediate product with 5 g / l00 g Brazil nuts paste 5. Intermediate product with 10 g / l 00 g Brazil nuts paste 6th Intermediate product with 20 g / l 00 g Brazil nuts paste 7th Intermediate product with 25 g / l 00 g Brazil nuts paste

Mice from 3-7. groups were treated with Brazil nuts paste - once a day for 20 min 7 days. The design of the experiment is shown in Table 1.

During the procedure, the animal was placed in a special cage (see Fig. B), which restricted the animal's ability to dispose of the test preparation. At the end of the application, the mice were wiped with a tissue and the animals were placed in a single cage (one cage per group). Animals were controlled for body weight dynamics. The condition of the dorsal hair of each animal was evaluated daily prior to application. The number of days until the hair regrowth to Group 2 (control) condition was recorded.

After 26 days, all animals were weighed, euthanized with ether. Mice from Groups 3, 6 and 7 were sampled (3 mice per group) for morphological examination. The cut pieces of leather were labeled, packed in cotton bags in a container containing 10% neutral formalin solution.

The biopaths were cassette-fed and processed in a histology machine and placed in a histoplasty. From the chilled histoplasty block, tissue sections were rotated using a microtome of tissue rotation, 2-4 pm thick, and the bundle was pulled and fixed on slides.

Histological sections were then stained with hematoxylin and eosin (review method) and by Van Gieson and Romeis methods.

The tissue incisions were stained with polystyrene after dyeing and clarification. Finished histological preparations were subjected to microscopic examination.

Table 2. Brazil nut growth inhibitory effect in mice.

Dec No, Brazil nut content in test composition, g / 100 g Number of days to complete hair restoration 1. Inactivated animals - 2. Feather Shaved (Control) 4 3. 0 4 4. 5 9th 5. 10th 12th 6th 20th 20th 7th 25th 20th

C D

1. The progress of the experiment.

A. Shaved mice.

B. Carrying out a Brazil nut application procedure.

C. Mouse from control group after 3 days.

D. Mouse from the group that tested the hair growth composition intermediate with 20% Brazil nut paste after 18 days.

As can be seen in Table 2, the maximal effect in inhibiting hair growth inhibition was provided by the intermediate of the new product with 20 g / 100 g and 25 g / 100 g of Brazil nuts.

The results of morphological examination of mouse skin are shown in Figure 2.

B

A

2. Hair Growth Inhibitor with Brazil Nut (20 g / 100 g)

(g) effects of contents on skin morphology. Microphoto. A. Hematoxylin -Eosin, x 100.

B. Van Gieson, x 100.

Epidermal plan with uneven wavy and shallow wavy recesses containing fragile keratin. Papermal, subpapillary, and reticular layer of the dermis compressed over a wide area of the stroma; fibroblasts in the stroma, with minimal lymphocyte infiltration around the blood vessels. Vascular lumen slit-like. Hair follicles and their sebaceous glands are normal.

Light microscope morphological studies have shown that Brazil nuts paste at concentrations of 20 g / 100 g and 25 g / 100 g did not cause toxic effects on skin structures, which indicates the safety of the product. The recovery time was similar for groups 6 and 7 (20 days). This means that the Brazil nut content of the new product could be limited to 20 g / 100 g.

The results of the study investigated the effect of the studied composition on the phases of the hair growth cycle, showing that Brazil nut nut mice reduced the amount of anagenic hair by 47% during the observation. The fact that microscopic hair follicles looked normal (Fig. 2) may indicate a malfunction of the hair follicle cells that make up the hair.

In addition, an experiment on mice was conducted to see if there was room for improvement

The ability of Brazil nut to inhibit hair growth in combination with other herbal products. The study evaluated water-alcohol extract of White willow bark and water-alcohol-glycerin extract of witch hazel. The following plant extracts were added to the composition:

Brazil nut paste (20%) - glycerol-emulsifier-water.

The design of the experiment was similar to that described above. We used only animals with clipped feathers. Groups of animals (n = 7):

1. Control

2. Bertholletia Excelsa Nut 20.0 g / 100 g, Glycerin 20 g / 100 g, Hydroxyethyl Acrylate / Sodium Aciyloyldimethyl Taurate Copolymer 2.0 g / 100 g, Aqua 58 g / 100 g

3. Salix Alba Bark Extract and Hamamelis Virginiana Extract (4: 1)

4. Bertholletia Excelsa Nut 20.0g / 100g, Salix Alba Bark Extract 8.0g / 100g, Hamamelis Virginiana Extract 2.0g / 100g, Glycerin 20g / 100g, Hydroxyethyl Acylate / Sodium Acryloyldimethyl Taurate Copolymer 2 , 0 g / 100 g, Aqua 48.0 g / 100 g.

The results of this experiment showed that combining Brazil nut paste with white willow bark and witch hazel extracts increased the time to complete restoration of mouse hair by 25%. The third group of mice regrowth after 18 days and the fourth group after 24 days, which is 4 days longer than Brazilian walnut paste (Group 2).

Based on the results of experiments carried out to increase the efficacy and further safety of the new product, the Brazil nut-glycerol-emulsifier water composition was supplemented with other substances (Table 4), which were used to prepare the final product and its two variants.

In the description, claims and summary text, the trade names of the components are used in the names of the ingredients of the composition.

Table 4. Characteristics of ingredients used in the preparation of hair growth inhibitors

No. Commodity Name, (INCI name) Characterization of raw materials 1 Bertholletia Excelsa Nut * Finely chopped Brazil nuts (pasta); inhibits hair growth 2 Salix Alba Bark Extract White-willow bark water-alcohol extract; inhibits hair growth 3 Hamamelis Virginiana Extract Witch hazel water-alcohol-glycerin extract; inhibits hair growth 4 Capiylic / Capric Triglyceride Soluble lipophilic components 5 All the way down Anti-inflammatory and anti-irritant effect 6th Urea Urea; moisturizes the skin, inhibits hair growth ⁷ , Glycerin Glycerine 99.5%; moisturizes and softens the skin

8th Ethoxydiglycol Transdermal substance transport activator 9th Menthol Menthol; anti-inflammatory, analgesic effect 10th Hydroxyethyl Acrylate / Sodium Aciyloyldimethyl Taurate Copolymer Emulsifier, gel maker 11th Cymbopogon Schoenanthus Oil Lemongrass oil, antimicrobial action, flavoring 12th BHT (Butylhydroxytoluene) Butylhydroxytoluene, antioxidant 13th Lavandula Augustifolia Oil Lavender essential oil, antimicrobial action, flavoring 14th Chamomilla Recutita Extract Chamomile alcohol-glycine extract 15th Cymbopogon Schoenanthus Oil Citrus essential oil, antimicrobial effect, flavoring 16th Rosmarinus Officinalis Oil Rosemary essential oil, antimicrobial effect, flavoring ¹⁷ , Aqua Water, purified; solvent

Volumetric observations were made to test the efficacy of the final formulation of the new product.

Option 1. Body Lotion with Menthol and Lemongrass Essential Oil for Hair Growth • Bertholletia Excelsa Nut 20.0g / L 00g • Salix Alba Bark Extract 8.0g / 100g • Hammam Virginiana Extract 2.0g / 100g • Caprylic / Capric Triglyceride 10.0 g / 100 g • Allantoin 0.30 g / 100 g • Urea 5.0 g / 100 g • Glycerin 2.5 g / 100 g • Ethoxydiglycol 15.0 g / 100 g • Menthol 0.5 g / 100 g • Methylchloroisothiazolinone, Methylisothiazolinone 0.07 g / 100 • Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 2.0 g / 100 g • Cymbopogon Schoenanthus Oil 0.4 g / 100 g • BHT 0.03 g / 100 g • Aqua 34, 20 g / 100 g

Variant 1 of the finished product was tested on 27 volunters. They were women 25-41 years old. v., with dark hair.

Before depilation, the hair on each shin (6 cm 2) was counted and extracted with 10 hairs for microscopic examination for growth phase identification (anagen or telogen). Both legs were depilated with hot wax. The new product, as well as comparison products, were applied on the left foot starting from the day of depilation. For a good leg, we used a placebo formula that did not contain Brazil nut. We use currently popular hair growth inhibitors for comparison. After 30 days from the first day of depilation, wax depilation was repeated and the course of application of the new agent was repeated according to the scheme described above.

Characteristics of Volontier groups (n = 9):

Group 1. New product, variant l (application for 20 min, once a day, 7 days, starting with depilation day; after 30 days the course was repeated)

Group 2. BX (used as directed - 2 times a day for 2 months); Ingredients Phaseolus vulgaris, Cucurbita pepo fruit, Cucumis sativus fruit, Citrus Auranf Amara fruit, Humulus Lupulus, Fagus Sylvatica extracts, Cetearyl Alcohol, Glycerin, 02-13 Alkyl Lactate, Stearic acid & Palmitic acid, Dimethicone, Ceteareth Methylparaben & 2-Bromo-2-Nitropropane -1,3-diol, Ascorbic Acid, Tocopherol Acetate, Parfum, BHT, Benzyl Alcohol & Methylchloroisothiazolinone & Methylisothiazolinone, Allantoin, Sodium Hydroxyde.

Group 3. K., (used as instructed - 2 times on depilatory day, 3 times on next day, repeating this regimen after 6 depilation procedures); spray after depilation; Ingredients - Aqua, Glycerol, Propylene Glycol, Sucrose, Persea Gratissima Oil, Lactic Acid, Citrus Grandīs Peel Extract, Chamomilla Recutita Flower / Leaf Extract, Betaglucan, Propylparaben, Methylparaben, Disodium EDTA.

At the end of the observation period, which lasted for 60 days, a quantitative evaluation of the hair of the lower leg skin was performed and microscopic examination of the extracted hair.

The results of a study on volontiers show that the new product is safe, does not irritate the skin and does not cause undesirable effects (Table 5).

Table 5. Results of a comparison of the specific activity of a new product and analogues of hair growth inhibitory cosmetics

Cosmetic tool The number of hair reduction during observation Reduction of the ratio of anagenic and telogenic hair follicles at the end of the course Evaluation of the effect of cosmetic product from the viewpoint of a volonther (n = 9) New product 75%, hair regrowth was thin and colorless 67.4% Note the effect 8 human beings BX 62%, hair regrowth was thin and colorless 59.2% Tagged 7 people K 60% 54.0% 7 people noted the effect

The results of experiments show that the developed hair growth inhibiting cream with menthol and lemongrass essential oils (basic recipe) is safe and more effective than analogues. Within 60 days, the new product reduced the number of regrowing hairs and changed the relationship between hair growth phases by an average of 75% on 8 of 9 volontiers.

As is known, there are morphological and physiological differences between hair in different parts of the body. The basic formula was modified in variants 2 and 3 to ensure the efficiency of the new product.

Option 2

Anti-Hair Growth Cream with Lavender Essential Oil and Chamomile Extract • Bertholletia Excelsa Nut 20.0g / 100g • Salix Alba Bark Extract 8.00g / 100g • Hammam Virginiana Extract 2.00g / 100g • Chamomilla Recutita Extract 0 , 50 g / 100 g • Capiylic / Capric Triglyceride 10.00 g / 100 g • Allantoin 0.35 g / 100 g • Urea 5.00 g / 100 g • Glycerin 2.0 g / 100 g • Ethoxydiglycol 15.00 g / 100 g • Methylchloroisothiazolinone, Methylisothiazolinone 0.07 g / 100 g • Lavandula Angustifolia Oil 0.50 g / 100 g • Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer 2.00 g / 100 g • BHT 0.03 g / 100 g • Aqua 34.25 g / 100 g

This cream is suitable for women for face care. The product was tested on 7 women aged 29-38 with enhanced hair above the upper lip. The cream was tested according to the scheme described above. According to the subjective evaluation of efficacy, the product provided a good effect; regrowth was thin, pale and invisible. Lavender essential oil and chamomile extract promoted a pleasant feeling after the procedure.

Men's facial hair is made up of bristles and their complete elimination is not urgent. However, hair growth inhibitors for men are on the market. For example, North for Men Stubble Minimizing Cream (ORIFLAME), Post-Shave Soother Beard Control Formula (CLINIQUE), etc. A Brazil nuts paste with a hair growth suppressant (variant 3) was developed.

Option 3

Menthol and Essential Oils for Men Hair Growth Formula • Bertholletia Excelsa Nut 20.00 g / 100 g • Salix AlbaBarkExtract8.00 g / 100 g • Hammam Virginiana Extract 2.00 g / 100 g • Caprylic / Capric Triglyceride 7.00 g / 100 g • Allantoin 0.40 g / 100 g • Urea 5.00 g / 100 g • Glycerin 2.50 g / 100 g • Ethoxydiglycol 18.00 g / 100 g • Menthol 0.50 g / 100 g • Methylchloroisothiazolinone, Methylisothiazolinone 0.07 g / 100 g • Cymbopogon Schoenanthus Oil 0.25 g / 100 g • Lavandula Angustifolia Oil 0.25 g / 100 g • Rosmarinus Officinalis Oil 0.50 g / 100 g • Hydroxyethyl Aciylate / Sodium Acryloyldimethyl Taurate Copolymer 2.00 g / 100 g • BHT 0.05 g / 100 g • Aqua 33.48 g / 100 g

The purpose of formulating this cream formula was to prepare a product for men on the basis of a base cream. The product was tested on 15 men aged 32-47, who shaved daily. The cream was applied daily after shaving for 60 days. Observations show that after 20 days the growth rate of hair started to decrease and after 30 days the need for shaving has decreased from 7 times a week to 4-5. After 60 days of application, the frequency of shaving has dropped to 2-3 times a week. Steady-state effects were observed in 12 of the 15 volunters.

Research has succeeded in creating a cosmetic product that suppresses hair growth in humans more effectively than other hair removal products on the market. The product is safe, improves skin condition and the user's well-being. As already noted, we associate the specific activity of Brazil nut with the elevated content of nut selenium. Thanks to the developed formula (mainly Ethoxydiglycol) active ingredients of the cream, incl. selenium / amino acid compounds are efficiently transported to the hair bulb through a channel formed after depilation. The result is an atypical -S-S- bond between the amino acids that make up the primary and spatial structure of the protein. The conformational changes of the protein molecules block the functions of hair-forming cells and hair growth.The hair growth inhibitory effect is provided not by the nut oil used in cosmetology, but by the nut tissue of the nut.

However, hair growth inhibitory effects of other ingredients of the new cream cannot be excluded. By combining Brazil nut with white willow bark and witch hazel extracts, synergism of hair growth inhibitory effect was achieved. The mechanism of this phenomenon is unclear. However, components of Hamomelis virginiana are known to have cytotoxic effects on skin cells (Tourino s.et al.Chem.Res.Toxicol., 2008,3,696704) as well as anti-inflammatory effects (Hughes-Formella BJ.et al.Dermatology, 1998,196,3,316-22). White willow bark contains salicylic acid with a pronounced anti-inflammatory effect similar to aspirin (see www.herbalremedies.com), although it does not explain the hair growth inhibitory effect. Salix alba is known in phytomedicine as a plant that promotes synergism and other interactions between other plants (Williamson

E.M.Phytomedicine, 2001,8,5,401-9).

On the basis of the base cream (ingredients of variant 1) two modifications of the new product were developed. One of them, with lavender essential oil and chamomile flower extract, is suitable for inhibiting hair growth on the face of women. The other - menthol as well as lemongrass, lavender and rosemary essential oils are effective for men after shaving. The three hair growth inhibitors not only provide a specific effect, but also improve the appearance of the skin and the well-being of the consumer.

Claims (7)

Claims A composition comprising, based on 100 g total weight, Brazil nuts (Bertholetia Excelsa Nut) 20.0 g, Caprylic / Capric Triglycerides (Caprylic / Capric Triglyceride) 10.0 g, Allantoin 0.30 g, urea (Urea) 5.0 g, glycerol (Glycerin) 2.5 g, ethoxydiglycol (ethoxydiglycol) 15.0 g, menthol (Menthol) 0.5 g, methylchloroisothiazolinone (Methylchloropisothiazolinone), methylisothiazolinone (Methylisothiazolinone) 0.07 g, hydroxyethyl acrylate / sodium acryloyldimethylturate copolymer (Hydroxyethyl Acrylate / Sodium Acryloildimethyl Taurate Copolymer) 2.0 g, Cymbopogon Schoenanthus Oil 0.4 g, Butylhydroxytoluene (Buthylhydroxytene), has the ability to inhibit hair growth. Composition according to Claim 1, characterized in that oil is extruded from the blanched and dried Brazil nuts using a thread press and a cold process, and the paste with a particle size of 30 to 50 micrometres is prepared. . Composition according to Claim 1, characterized in that it contains Brazil nuts (Bertholetia Excelsa) paste in the amount of 20.0 g per 100 g of the composition. Composition according to claim 1, characterized in that it additionally contains 8.0 g of white willow bark (Salīx Alba Bark) and 2.0 g per 100 g of the composition of Virginia witch extract (Hamamelis Virginiana Extract). amount of water in the composition up to 24.2 g. Use of a composition according to claim 1 or 2 for inhibiting the growth of body hair. Composition according to Claim 1 or 2, characterized in that, instead of menthol (Menthol) and lemongrass oil (Cymbopogon Schoenanthus Oil), it contains 0.50 g per 100 g of chamomile Recutita Extract and healing. lavender oil (Lavandula angustifolia Oil) 0.50 g per 100 g, which has the ability to inhibit hair growth on the face of women. Composition according to Claim 1 or 2, characterized in that it additionally contains 0.25 g per 100 g of lavender oil (Lavandula angustifolia Oil) and 0.50 g per rosemary essential oil (Rosmarinus Officinalis Oil). 100 g, which has the ability to inhibit the growth of hair on the face of men and improve the appearance and elasticity of the skin.