LU504233B1

LU504233B1 - A Tea Drink with Anti-Alcohol and Liver-Protecting Effects and Preparation Method Thereof

Info

Publication number: LU504233B1
Application number: LU504233A
Authority: LU
Inventors: xiao ping Wang; Yan Li; Jian Xin Chai; Ying Li; An Ping Li
Original assignee: Shanxi Zhendong Wu He Yi Yang Tang Co Ltd
Priority date: 2023-05-16
Filing date: 2023-05-16
Publication date: 2023-11-16

Abstract

The invention discloses a tea drink with anti-alcohol and liver-protecting effects, which belongs to the technical field of health care products, and comprises the following raw materials in parts by weight: 3-4 parts of Hovenia dulcis, 1-2 parts of Pueraria lobata, 1-2 parts of mulberries (Fructus Mori), 1-2 parts of chrysanthemum (chrysanthemum morifolium), 1-2 parts of Pericarpium citri reticulatae and 1-3 parts of Pu-er ripe tea. The preparation method comprises the following steps: weighing the raw materials; mixing Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae, and Pu-er ripe tea, placing in an extraction tank, adding hot water for extraction, and then adding mulberries for continuous extraction to obtain a mixture; Centrifuging the mixture, collecting supernatant, concentrating, and drying to obtain tea.

Description

DESCRIPTION LUS04233

A Tea Drink with Anti-Alcohol and Liver-Protecting Effects and Preparation Method

Thereof

TECHNICAL FIELD

The invention belongs to the technical field of health care products, and more specifically relates to a tea drink with anti-alcohol and liver-protecting effects and a preparation method thereof.

BACKGROUND

With the continuous improvement of people's living standards, the number and quantity of drinkers in China are on the rise, although a small amount of drinking can promote blood circulation, dredge collaterals and dispel cold, adjust mood and eliminate fatigue and tension;

However, excessive drinking and excessive drinking will cause pathological damage to many tissues and organs including cardiovascular system, nervous system and digestive system, and even lead to death due to respiratory muscle paralysis in severe cases. Investigation shows that among patients who drink alcohol for a long time, about 57.5% patients have fatty liver and 15% patients have cirrhosis. With the increase of alcohol consumption and the extension of drinking time, the harm of alcohol to the liver gradually develops according to the trilogy of "alcoholic fatty liver, alcoholic hepatitis and alcoholic cirrhosis". By affecting the metabolism of the liver, the lipid components on the surface of the hepatocyte membrane are over-oxidized, thus damaging the hepatocyte membrane and leading to the destruction of hepatocytes. This process is an important link for alcohol to damage the liver.

Therefore, it is an urgent problem for technicians in this field to develop a tea with obvious efficacy, long-term consumption, anti-alcohol and liver-protecting effects and its preparation method.

SUMMARY LU504233

In view of this, the invention provides a tea which has obvious efficacy, can be consumed for a long time, and has the efficacy of anti-alcohol and liver-protecting, and a preparation method thereof.

In order to achieve the above purpose, the invention adopts the following technical scheme:

A tea drink with anti-alcohol and liver-protecting effects comprises the following raw materials in parts by weight: 3-4 parts of Hovenia dulcis, 1-2 parts of Pueraria lobata, 1-2 parts of mulberries, 1-2 parts of chrysanthemum, 1-2 parts of Pericarpium citri reticulatae and 1-3 parts of Pu-er ripe tea.

The invention has the beneficial effects that according to the theory of Traditional Chinese

Medicine Formulation, the Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum and

Pericarpium citri reticulatae, which are homologous in medicine and food, are compounded to prepare a tea with the efficacy of anti-alcohol and liver-protecting, which not only has excellent performance in sensory quality, such as color, taste and fragrance, but also increases the enjoyment of the tea drink; Experiments are carried out in zebrafish, a model organism, which shows that the tea of the invention has obvious efficacy, and people can achieve the effect of anti-alcohol and liver-protecting after taking it for a long time, so it is suitable for popularization and application.

In addition, the effective components in Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and other plant sources, which are homologous to medicine and food, have good medicinal functions of anti-alcohol and liver-protecting.

Hovenia dulcis contains loline and Hovenin, which has the effects of promoting diuresis, reducing swelling and anti-alcohol.

Pueraria lobata, as prescribed in China Pharmacopoeia 2015, is sweet, pungent and cool.

Concerning spleen, stomach and lung meridians. Able to relieve muscle and fever, promote fluid production to quench thirst, penetrate rash, raise yang to stop diarrhea, dredge channels and activate collaterals, and relieve alcoholism. It can be used for treating exogenous fever, headache, strong nape pain, thirst-quenching, measles, dysentery, diarrhea, dizziness, stroke, hemiplegia, chest obstruction, heartache, and alcoholic injury.

Mulberries are warm and sweet, and has the functions of nourishing liver and kidney/°04233 nourishing blood and promoting fluid production;

Chrysanthemum 1s rich in volatile oil, flavonoids, amino acids and other components. It can dilate the coronary artery, increase the arterial blood flow and improve the tolerance of myocardial cells to hypoxia;

Pericarpium citri reticulatae is rich in volatile oil, which can cure stomach Qi rising, nausea and vomiting.

According to the invention, medicinal and edible raw materials are selected, and through scientific dialectical compatibility and mutual coordination, the efficacy of the composition in anti-alcohol and liver-protecting is jointly promoted. In the composition of the invention,

Hovenia dulcis, Pueraria lobata and mulberries are used as components for resisting oxidation, liver-protecting and anti-alcohol, while Pericarpium citri reticulatae, chrysanthemum and Pu-er ripe tea are used as components for assisting in liver-protecting and anti-alcohol and adjusting flavor, and through compounding and synergism, the efficacy of anti-alcohol and liver-protecting of the composition is jointly promoted, so that the composition with good effect of anti-alcohol and liver-protecting is obtained.

In addition, in the prior art, although the common dosage of each Chinese medicine is known, the prescription is an organic whole after compatibility of multiple medicines according to the symptoms, and its efficacy is not equal to the simple superposition of each medicine on the basis of the common dosage. According to the common dosage of each Chinese medicine, the dosage in the prescription is usually not determined, and the dosage ratio of the medicine is determined by various considerations such as the characteristics of the medicine, the theory of

Traditional Chinese Medicine Formulation, and so on, and it is not simply optimized by experimental means such as comparative method and orthogonal method.

The invention also provides a preparation method of the tea drink with anti-alcohol and liver-protecting effects, which comprises the following steps: (1) Weigh the raw materials according to the described tea drink with anti-alcohol and liver-protecting effects; (2) Mix Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and Pu-er ripe tea, put them into an extraction tank, add hot water for extraction, and then add mulberries for continuous extraction to obtain a mixture; LU504233 (3) Centrifuge the mixture, collect supernatant, concentrate and dry to obtain the tea.

The invention has the beneficial effects of simple preparation method, convenient operation and suitability for large-scale production and application.

Furthermore, in step (2), the temperature of the hot water is 95°C.

The adoption of the further technical scheme has the beneficial effects that by adding hot water for extraction, some components insoluble in cold water can be fully extracted in a limited time.

Furthermore, in step (2), hot water is added for extraction, the temperature of the extraction tank is 90-95°C, and the extraction time is 1h.

The adoption of the further technical scheme has the beneficial effects that the extraction process selected by the invention can better extract effective components and exert the maximum efficacy.

Furthermore, in step (2), mulberries are added and the extraction is continued, and the temperature of the extraction tank is 90-95°C, and the extraction time is 30min.

The adoption of the further technical scheme has the beneficial effects that the selected extraction process can better maintain the nutritional value of the mulberries, and at the same time, the original taste and color of the mulberries can be avoided from being changed due to too long extraction time.

Furthermore, in step (3), the centrifugal speed is 19000rpm and the centrifugal time is 2.5h.

The adoption of the above further technical scheme has the beneficial effects that higher rotating speed can make the tea soup color clearer, and long-term centrifugation can keep the materials slowly entering the centrifuge, so that the impurities in it can be fully separated.

Furthermore, in step (3), the above concentration is carried out by using low-temperature reverse osmosis membrane, the concentration temperature is 25-35°C, the concentration time is 2h, and the concentration of the concentrated solution is 9.4%.

The adoption of the further technical scheme has the beneficial effects that the original taste and nutritional value of the materials can be better maintained through concentration.

Furthermore, in step (3), the drying temperature is 156°C and the drying time is 8h.

The adoption of the further technical scheme has the beneficial effects that the moisture hp 904233 the material is more likely to volatilize in a high-temperature environment to form powder, and the drying time can be controlled so that the material can slowly enter the spray drier and the dryness of the powder can be maintained.

Furthermore, it also includes step (4): making the tea into powder, granule, pill or tablet.

The adoption of the above further technical scheme has the beneficial effects that it can be carried and consumed more conveniently.

The invention also provides an application of the tea drink with anti-alcohol and liver-protecting effects in preparing health food.

BRIEF DESCRIPTION OF THE FIGURES

In order to clearly explain the embodiment of the present invention or the technical scheme in the prior art, the figures needed in the description of the embodiment or the prior art will be briefly introduced below. Obviously, the figures in the following description are only the embodiment of the present invention, and other figures can be obtained according to the provided figures without creative labor for ordinary people in the field.

Fig. 1 is a typical diagram of zebrafish behavior track after tea treatment (black line: slow movement; gray line: fast movement; the rest: medium-speed movement)

Fig. 2 is a comparison diagram of the total movement distance of zebrafish after tea treatment of the present invention and the model control group, **p<0.01, ***p<0.001.

Fig. 3 is a diagram of the anti-alcohol effect of the tea of the present invention.

Fig. 4 is a typical diagram of the liver fat staining intensity of zebrafish after tea treatment of the present invention.

Fig. 5 is a comparison diagram of the liver fat staining intensity of zebrafish after tea treatment of the present invention and the model control group, ***p<0.001.

Fig. 6 is a diagram showing the protective effect of the tea of the present invention on alcoholic fatty liver.

DETAILED DESCRIPTION OF THE INVENTION 0504238

The technical scheme in the embodiment of the present invention will be described clearly and completely below. Obviously, the described embodiment is only a part of the embodiment of the present invention, but not the whole embodiment. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without creative labor belong to the scope of protection of the present invention.

Embodiment 1

The preparation method of the tea drink with anti-alcohol and liver-protecting effects comprises the following steps: (1) Weigh 4kg of Hovenia dulcis, 2kg of Pueraria lobata, 1kg of mulberries, 1kg of chrysanthemum, 1kg of Pericarpium citri reticulatae and 3kg of Pu-er ripe tea; (2) Mix Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and Pu-er ripe tea, put them into an extraction tank, add 95°C hot water for extraction for 1h, the temperature of the extraction tank is 95 °C, then add mulberries for continuous extraction for 30min, the temperature of the extraction tank is 95 °C, afterwards, obtain the mixture; (3) Centrifuge the mixture at the speed of 19000rpm for 2.5h, take the supernatant, concentrate it for 2h with low-temperature reverse osmosis membrane at 25°C until the concentration of the concentrated solution is 9.4%, and dry it at 156°C for 8h to obtain the tea drink.

Embodiment 2

The preparation method of the tea drink with anti-alcohol and liver-protecting effects comprises the following steps: (1) Weigh 3kg of Hovenia dulcis, 1kg of Pueraria lobata, 2kg of mulberries, 1kg of chrysanthemum, 2kg of Pericarpium citri reticulatae and 1kg of Pu-er ripe tea; (2) Mix Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and Pu-er ripe tea, put them into an extraction tank, add 95°C hot water for extraction for 1h, the temperature of the extraction tank is 90 °C, then add mulberries for continuous extraction for 30min, the temperature of the extraction tank is 90 °C, afterwards, obtain the mixture;

(3) Centrifuge the mixture at the speed of 19000rpm for 2.5h, take the supernatarit) 204233 concentrate it for 2h with low-temperature reverse osmosis membrane at 35°C until the concentration of the concentrated solution is 9.4%, and dry it at 156°C for 8h to obtain the tea drink.

Embodiment 3

The preparation method of the tea drink with anti-alcohol and liver-protecting effects comprises the following steps: (1) Weigh 3kg of Hovenia dulcis, 1kg of Pueraria lobata, 2kg of mulberries, 1kg of chrysanthemum, 1kg of Pericarpium citri reticulatae and 2kg of Pu-er ripe tea; (2) Mix Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and Pu-er ripe tea, put them into an extraction tank, add 95°C hot water for extraction for 1h, the temperature of the extraction tank is 92 °C, then add mulberries for continuous extraction for 30min, the temperature of the extraction tank is 92 °C, afterwards, obtain the mixture; (3) Centrifuge the mixture at the speed of 19000rpm for 2.5h, take the supernatant, concentrate it for 2h with low-temperature reverse osmosis membrane at 30°C until the concentration of the concentrated solution is 9.4%, and dry it at 156°C for 8h to obtain the tea drink.

In the following experiments, the Pueraria lobata Hovenia dulcis tea refers to the tea of the embodiment of the invention.

Experiment 1 of Pueraria lobata Hovenia dulcis tea: Experiment on anti-alcohol effect 300 Sdpf wild-type AB strain zebrafish are randomly selected in a six-well plate, and treat zebrafish in each experimental group, and then give them ethanol in water to establish a drunken model of zebrafish during the exciting period. The concentrations of Pueraria lobata

Hovenia dulcis tea in Embodiment 3 are 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000pug/mL respectively, and the positive control group "Ru21" is 100ug/mL. At the same time, the normal control group and the model control group are set up, and the capacity of each well is 3ml. After the treatment: 1) Observe and count the number of zebrafish deaths and toxic reactions in each experimental group, and determine the maximum test concentration (MTC) of Pueraria lobata

Hovenia dulcis tea; 2) Three concentration groups (MTC and below), positive control group,

model control group and normal control group are selected, and 12 zebrafish are randomly’ 504233 selected from each group. The total movement distance (S) of zebrafish is analyzed with the detection software of behavior analyzer, and the anti-alcohol effect of Pueraria lobata Hovenia dulcis tea is evaluated with the statistical analysis results of the total movement distance. The statistical results are expressed by mean+SE. The formula of anti-alcohol effect is as follows:

Statistical analysis is made by variance analysis and Dunnett'sT- ST-test, p<0.05 means that there is significant difference.

Table 1 Experimental Results of Anti-alcohol Concentration of Pueraria lobata Hovenia dulcis Tea (n = 30)

Under the experimental conditions, the MTC of Pueraria lobata Hovenia dulcis Tea is 2500ug/mL.

Table 2 Total Movement Distance of Zebrafish Treated with Pueraria lobata Hovenia dulcis

Tea (n= 12)

Compared with the model control group, **p<0.01, ***p<0.001.

Table 2 shows that Pueraria lobata Hovenia dulcis Tea has remarkable anti-alcohol effect.

Experiment 2: Protective effect test of alcoholic fatty liver

Randomly select 270 Sdpf melanin allele mutated semi transparent Albino strains of zebrafish in a six well plate, and 30 zebrafish are treated in each experimental group. The model of alcoholic fatty liver of zebrafish is established by dissolving in water and giving ethanol. The concentrations of Pueraria lobata Hovenia dulcis Tea in Embodiment 3 are 78.1, 156.3, 312.5, 625, 1250, 2500 and 5000pg/mL, respectively. At the same time, the normal control group and the model control group are set up, and the capacity of each well is 3ml. After the treatment: 1)

Observe and count the number of zebrafish deaths and toxic reactions in each experimental group, and determine the MTC of Pueraria lobata Hovenia dulcis Tea, 2) Take three concentration groups (MTC and below), positive control group, model control group and norma’ 204233 control group, and randomly select 10 zebrafish in each group to take photos under dissecting microscope, analyze and collect data with NIS-ElementsD3.10 advanced image processing software, and analyze the intensity (S) of liver fat staining of zebrafish, and evaluate the protective effect of Pueraria lobata Hovenia dulcis Tea on alcoholic fatty liver with the statistical analysis results of liver fat staining intensity. The statistical results are expressed by mean+SE.

The formula for calculating the protective effect of alcoholic fatty liver is as follows:

Table 3 Experimental Results of Protective Concentration of Pueraria lobata Hovenia dulcis Tea on Alcoholic Fatty Liver (n = 30)

Table 4 The Intensity of Fat Staining in The Liver of Zebrafish Treated with Pueraria lobata Hovenia dulcis Tea (n = 10)

Compared with the model control group, ***p<0.001.

Table 4 shows that Pueraria lobata Hovenia dulcis Tea has a significant protective effect on alcoholic fatty liver.

The description of the disclosed embodiments enables those skilled in the art to make or use the invention. Many modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

CLAIMS LU504233

1. A tea drink with anti-alcohol and liver-protecting effects, which is characterized by comprising the following raw materials in parts by weight: 3-4 parts of Hovenia dulcis, 1-2 parts of Pueraria lobata, 1-2 parts of mulberries, 1-2 parts of chrysanthemum, 1-2 parts of Pericarpium citri reticulatae and 1-3 parts of Pu-er ripe tea.

2. A method for preparing the tea drink with anti-alcohol and liver-protecting effects, which comprises the following steps: (1) weigh the raw materials of the tea drink with anti-alcohol and liver-protecting effects according to claim 1; (2) mix Hovenia dulcis, Pueraria lobata, mulberries, chrysanthemum, Pericarpium citri reticulatae and Pu-er ripe tea, put them into an extraction tank, add hot water for extraction, and then add mulberries for continuous extraction to obtain a mixture: (3) centrifuge the mixture, collect supernatant, concentrate and dry to obtain the tea.

3. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (2), the temperature of the hot water is 95°C.

4. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (2), 95°C hot water is added for extraction, the extraction tank temperature is 90-95°C, and the extraction time 1s 1h.

5. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (2), mulberries are added to continue extraction, the extraction tank temperature is 90-95°C, and the extraction time is 30min.

6. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (3), the centrifugal speed is 19000rpm and the centrifugal time is 2.5h.

7. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (3), low-temperature reverse osmosis membrane concentration is adopted, the concentration temperature is 25-35°C, the concentration time is 2h, and the concentration of concentrated solution is 9.4%.

8. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that in step (3), the drying temperature is 156°C and the drying time is 8h. LU504233

9. The preparation method of tea drink with anti-alcohol and liver-protecting effects according to claim 2, characterized in that it further comprises step (4): making the tea into powder, granules, pills or tablets.

10. An application of the tea drink with anti-alcohol and liver-protecting effects as claimed in claim 1 in preparing health food.