LU502571B1 - Microfluidic chip integrating exosome separation and detection and method thereof - Google Patents

Microfluidic chip integrating exosome separation and detection and method thereof Download PDF

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Publication number
LU502571B1
LU502571B1 LU502571A LU502571A LU502571B1 LU 502571 B1 LU502571 B1 LU 502571B1 LU 502571 A LU502571 A LU 502571A LU 502571 A LU502571 A LU 502571A LU 502571 B1 LU502571 B1 LU 502571B1
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LU
Luxembourg
Prior art keywords
separation
exosomes
detection
area
antibody
Prior art date
Application number
LU502571A
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English (en)
Inventor
Yongyu Liu
Kun Deng
Xuedong Tong
Yong Zhang
Original Assignee
The Third Affiliated Hospital Of Cqmu Gener Hospital
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Publication date
Application filed by The Third Affiliated Hospital Of Cqmu Gener Hospital filed Critical The Third Affiliated Hospital Of Cqmu Gener Hospital
Priority to LU502571A priority Critical patent/LU502571B1/en
Application granted granted Critical
Publication of LU502571B1 publication Critical patent/LU502571B1/en

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4915—Blood using flow cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Claims (7)

PATENTANSPRUCHE LU502571
1. Fin Mikrofluidik-Chip, integrierend von Trennung und Nachweis von Exosomen, dadurch gekennzeichnet, dass der einen Probeneinlass, einen Trennbereich, einen Nachweisbereich und einen Qualitätskontrollbereich gemäB der Anschlussreihenfolge umfasst; der Kanal des Trennbereichs ist mit einem Antikôrper zum Einfangen von Exosomen beschichtet, der Nachweisbereich ist mit einem GPC-1-Antikôrper beschichtet, und der Qualitätskontrollbereich ist mit einem CD63-Antikôrper beschichtet.
2. Der Mikrofluidik-Chip, integrierend der Trennung und des Nachweises von Exosomen, nach Anspruch 1, dadurch gekennzeichnet, dass der Trennbereich Array-Einheiten umfasst, bestehend aus … Fischgräten-Mikrokanälen, jede Array-Einheit besteht aus acht einzelnen Fischgräten-Mikrokanälen, und die acht Fischgräten-Mikrokanäle sind durch Rohrverbinder mit dem Probeneinlass verbunden.
3. Der Mikrofluidik-Chip, integrierend der Trennung und des Nachweises von Exosomen, nach Anspruch 1, dadurch gekennzeichnet, dass der durch den Kanal beschichtete Antikôrper Tim-4 ist.
4. Fin Verfahren zur Abtrennung von Exosomen durch Chip nach Anspruch 1, dadurch gekennzeichnet, dass es umfasst: Zugabe einer Kôrperflüssigkeitsprobe in einen Probeneinlass, Einfangen der Exosomen in der Kôrperflüssigkeitsprobe durch einen Einfang-Antikôrper, beschichtet im Kanal des Chips, und anschlieBendes Eluieren mit Elutionsmittel, um die Exosomen zu erhalten.
5. Das Verfahren nach Anspruch 4, dadurch gekennzeichnet, dass die Injektionsflussrate der Kôrperflüssigkeitsprobe 20 uL/min beträgt.
6. Das Verfahren nach Anspruch 4, dadurch gekennzeichnet, dass das Eluent ein Glycin-HCI-Puffer mit einem pH-Wert von 2,8-8,5 ist.
7. Das Verfahren nach Anspruch 6, dadurch gekennzeichnet, dass die Flussrate des Eluenten 80 uL/min beträgt.
LU502571A 2022-07-27 2022-07-27 Microfluidic chip integrating exosome separation and detection and method thereof LU502571B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
LU502571A LU502571B1 (en) 2022-07-27 2022-07-27 Microfluidic chip integrating exosome separation and detection and method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LU502571A LU502571B1 (en) 2022-07-27 2022-07-27 Microfluidic chip integrating exosome separation and detection and method thereof

Publications (1)

Publication Number Publication Date
LU502571B1 true LU502571B1 (en) 2023-01-27

Family

ID=84982803

Family Applications (1)

Application Number Title Priority Date Filing Date
LU502571A LU502571B1 (en) 2022-07-27 2022-07-27 Microfluidic chip integrating exosome separation and detection and method thereof

Country Status (1)

Country Link
LU (1) LU502571B1 (de)

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Effective date: 20230127