LU100734B1 - Synthetic transfer RNA with extended anticodon loop - Google Patents
Synthetic transfer RNA with extended anticodon loop Download PDFInfo
- Publication number
- LU100734B1 LU100734B1 LU100734A LU100734A LU100734B1 LU 100734 B1 LU100734 B1 LU 100734B1 LU 100734 A LU100734 A LU 100734A LU 100734 A LU100734 A LU 100734A LU 100734 B1 LU100734 B1 LU 100734B1
- Authority
- LU
- Luxembourg
- Prior art keywords
- trna
- anticodon
- base
- transfer rna
- synthetic
- Prior art date
Links
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
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- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (8)
1. Synthetische Transfer-RNA, umfassend eine erweiterte Anticodon-Schleife mit zweiaufeinanderfolgenden Anticodon-Basentripletts, die in der Lage sind, mit zweiaufeinanderfolgenden Codon-Basentripletts auf einer mRNA eine Basenpaarung einzugehen,wobei das erste Anticodon-Basentriplett oder das zweite Anticodon-Basentriplett in der Lageist, mit einem Stopp-Codon-Basentriplett auf der mRNA eine Basenpaarung einzugehen.
2. Synthetische Transfer-RNA nach Anspruch 1, wobei die Anticodon-Schleife aus 9 oder10 Nucleotiden besteht.
3. Synthetische Transfer-RNA nach Anspruch 1 oder 2, wobei die Transfer-RNAaminoacyliert ist.
4. Synthetische Transfer-RNA nach Anspruch 3, wobei die Transfer-RNA mit einemDipeptid aminoacyliert ist.
5. Synthetische Transfer-RNA nach einem der vorhergehenden Ansprüche, wobei diesynthetische Transfer-RNA a) eine der Sequenzen gemäß SEQ ID NO: 03-07, 09-17 oder 19-23 hat oder umfasst, oder b) eine Sequenz mit mindestens 90%, bevorzugt mindestens 95%,96%, 97%, 98% oder 99% Sequenzidentität mit einer der Sequenzen gemäß SEQ ID NO: 03-07,09-17 oder 19-23 hat oder umfasst, oder c) eine Sequenz gemäß einer der SEQ ID NO: 03-07, 09-17 oder 19-23, bei der mindestens eines der Nucleotide mit einem entsprechendenmodifizierten Nucleotid ersetzt ist, hat oder umfasst.
6. Synthetische Transfer-RNA nach einem der vorhergehenden Ansprüche zur Verwendungals Arzneimittel.
7. Synthetische Transfer-RNA nach einem der Ansprüche 1 bis 5 zur Verwendung alsMedikament bei einer Krankheit, die zumindest teilweise durch eine Nonsense-Mutationverursacht ist, die zu einem vorzeitigen Abbruch der Translation einer mRNA fuhrt.
8. Synthetische Transfer-RNA nach einem der Ansprüche 1 bis 5 zur Verwendung alsArzneimittel zur Behandlung von Cystischer Fibrose, einer Muskeldystrophie des TypsDuchenne oder Neurofibromatose Typ 1.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU100734A LU100734B1 (en) | 2018-03-15 | 2018-03-15 | Synthetic transfer RNA with extended anticodon loop |
| EP19710419.3A EP3765609A1 (de) | 2018-03-15 | 2019-03-14 | Synthetische transfer-rns mit verlängerter anticodon-schleife |
| PCT/EP2019/056429 WO2019175316A1 (en) | 2018-03-15 | 2019-03-14 | Synthetic transfer rna with extended anticodon loop |
| JP2020549042A JP7578490B2 (ja) | 2018-03-15 | 2019-03-14 | 拡張アンチコドンループを有する合成転移ｒｎａ |
| CA3094010A CA3094010A1 (en) | 2018-03-15 | 2019-03-14 | Synthetic transfer rna with extended anticodon loop |
| US16/980,927 US11434485B2 (en) | 2018-03-15 | 2019-03-14 | Synthetic transfer RNA with extended anticodon loop |
| AU2019233673A AU2019233673B2 (en) | 2018-03-15 | 2019-03-14 | Synthetic transfer RNA with extended anticodon loop |
| US17/876,803 US12077754B2 (en) | 2018-03-15 | 2022-07-29 | Synthetic transfer RNA with extended anticodon loop |
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| LU100734A LU100734B1 (en) | 2018-03-15 | 2018-03-15 | Synthetic transfer RNA with extended anticodon loop |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030224479A1 (en) | 2001-10-16 | 2003-12-04 | Uttam L. Rajbhandary | Suppressor tRNA system |
| WO2005007870A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF |
| US20060177900A1 (en) | 2003-07-07 | 2006-08-10 | The Scripps Research Institute | Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof |
-
2018
- 2018-03-15 LU LU100734A patent/LU100734B1/en active IP Right Grant
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030224479A1 (en) | 2001-10-16 | 2003-12-04 | Uttam L. Rajbhandary | Suppressor tRNA system |
| US6964859B2 (en) | 2001-10-16 | 2005-11-15 | Massachusetts Institute Of Technology | Suppressor tRNA system |
| WO2005007870A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF |
| US20060177900A1 (en) | 2003-07-07 | 2006-08-10 | The Scripps Research Institute | Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof |
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