LTIP462A - Hepatitis b virus surface proteins with reduced host carbohydrate content - Google Patents
Hepatitis b virus surface proteins with reduced host carbohydrate contentInfo
- Publication number
- LTIP462A LTIP462A LTIP462A LTIP462A LTIP462A LT IP462 A LTIP462 A LT IP462A LT IP462 A LTIP462 A LT IP462A LT IP462 A LTIP462 A LT IP462A LT IP462 A LTIP462 A LT IP462A
- Authority
- LT
- Lithuania
- Prior art keywords
- hbv
- surface proteins
- hepatitis
- carbohydrate content
- particles
- Prior art date
Links
- 241000700721 Hepatitis B virus Species 0.000 title abstract 7
- 102000018697 Membrane Proteins Human genes 0.000 title abstract 4
- 108010052285 Membrane Proteins Proteins 0.000 title abstract 4
- 150000001720 carbohydrates Chemical class 0.000 title abstract 3
- 239000002245 particle Substances 0.000 abstract 3
- 108700026244 Open Reading Frames Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 210000002845 virion Anatomy 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/29—Hepatitis virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/01—DNA viruses
- C07K14/02—Hepadnaviridae, e.g. hepatitis B virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Oncology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
Abstract
In order to produce hepatitis B virus (HBV) surface proteins in the form of particles with substantially reduced entrapped carbohydrate content, DNA encoding the HBV surface proteins was expressed in a recombinant yeast host which is deficient in its ability to glycosylate proteins. These HBV surface proteins display the antigenic sites genetically encoded by the S domain of the HBV virion envelope open reading frame and contains substantially reduced levels of entrapped carbohydrate when compared with HBsAg particles produced in "wild-type" yeast cells. These particles are useful as a vaccine for both the active and passive treatment or prevention of disease and/or infection caused by HBV or other agents serologically related to HBV. <IMAGE>
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69292491A | 1991-04-29 | 1991-04-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LTIP462A true LTIP462A (en) | 1994-10-25 |
| LT3367B LT3367B (en) | 1995-08-25 |
Family
ID=24782605
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTIP462A LT3367B (en) | 1991-04-29 | 1993-03-30 | Hepatitis b virus surface proteins with reduced host carbohydrate content |
Country Status (25)
| Country | Link |
|---|---|
| US (1) | US5614384A (en) |
| EP (1) | EP0516286B1 (en) |
| JP (1) | JP2637877B2 (en) |
| KR (1) | KR920019373A (en) |
| CN (1) | CN1067680A (en) |
| AT (1) | ATE215987T1 (en) |
| AU (1) | AU650530B2 (en) |
| BG (1) | BG61700B1 (en) |
| CA (1) | CA2067290A1 (en) |
| CZ (1) | CZ282769B6 (en) |
| DE (1) | DE69232541D1 (en) |
| FI (1) | FI934650A7 (en) |
| HU (1) | HUT69149A (en) |
| IE (1) | IE921385A1 (en) |
| IL (1) | IL101651A0 (en) |
| LT (1) | LT3367B (en) |
| MX (1) | MX9201970A (en) |
| NO (1) | NO933897L (en) |
| NZ (1) | NZ242487A (en) |
| PL (1) | PL171485B1 (en) |
| SG (1) | SG55152A1 (en) |
| SK (1) | SK118193A3 (en) |
| WO (1) | WO1992019741A1 (en) |
| YU (1) | YU45492A (en) |
| ZA (1) | ZA923069B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0533263A3 (en) * | 1991-09-20 | 1994-06-08 | Merck & Co Inc | A multivalent hepatitis b virus vaccine |
| AU2515095A (en) * | 1994-05-16 | 1995-12-05 | Merck & Co., Inc. | Process for producing hbv surface proteins |
| US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6300065B1 (en) * | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| EP1140155A4 (en) * | 1998-12-23 | 2004-11-03 | Merck & Co Inc | IMPROVED RECOMBINATION HEPATITIS B ENVELOPE ANTIGEN |
| KR100604994B1 (en) * | 2004-01-30 | 2006-07-26 | 한국생명공학연구원 | Method for producing recombinant glycoproteins using a novel gene of Hanshenula polymorpha encoding alpha 1,6-mannosyltransferase and Hanshenula polymorpha mutant lacking the gene |
| CN111187720A (en) * | 2019-12-27 | 2020-05-22 | 深圳康泰生物制品股份有限公司 | Cell disruption method of recombinant hansenula polymorpha expressing enterovirus 71-type antigen and application of cell disruption method in preparation of hand-foot-and-mouth disease vaccine |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4803164A (en) * | 1981-08-31 | 1989-02-07 | Genentech, Inc. | Preparation of hepatitis b surface antigen in yeast |
| US4707542A (en) * | 1983-08-22 | 1987-11-17 | Merck & Co., Inc. | Immunogenic HbsAg derived from transformed yeast |
| US4816564A (en) * | 1986-01-31 | 1989-03-28 | Merck & Co., Inc. | Method for producing hepatitis B virus proteins in yeast |
| GB8613388D0 (en) * | 1986-06-03 | 1986-07-09 | Delta Biotechnology Ltd | Induction of galactose regulated gene expression in yeast |
| DE3719213C1 (en) * | 1987-06-09 | 1988-05-19 | Daimler Benz Ag | Ground-level multi-pole power supply for trackable, rubber-tired, electrically driven vehicles |
| US4963483A (en) * | 1987-10-13 | 1990-10-16 | Merck & Co., Inc. | Method for producing hepatitis B virus proteins in yeast |
| US5135854A (en) * | 1987-10-29 | 1992-08-04 | Zymogenetics, Inc. | Methods of regulating protein glycosylation |
| EP0314096B1 (en) * | 1987-10-29 | 1994-01-12 | Zymogenetics, Inc. | Methods of regulating protein glycosylation |
| GB8726953D0 (en) * | 1987-11-18 | 1987-12-23 | Delta Biotechnology Ltd | Yeast expression system |
| NZ229260A (en) * | 1988-06-03 | 1994-02-25 | Merck & Co Inc | Hepatitis b virus, expression cassette for pre-s domain, host cells and |
| ATE159031T1 (en) * | 1989-07-25 | 1997-10-15 | Smithkline Biolog | ANTIGENS AND METHODS FOR THE PRODUCTION THEREOF |
| CA2022109A1 (en) * | 1989-07-31 | 1991-02-01 | Cheryl A. Schulman | Enhanced expression of heterologous proteins in recombinant hosts in a non-growth media |
| CA2022108A1 (en) * | 1989-07-31 | 1991-02-01 | Cheryl A. Schulman | Enhanced growth and expression of heterologous proteins in recombinant hosts containing mutations in cell wall and/or plasma membrane biosynthesis |
| DE69013471T2 (en) * | 1989-12-05 | 1995-03-30 | Merck & Co Inc | Method for stabilizing recombinant hepatitis B virus surface proteins from yeast. |
| IL101653A0 (en) * | 1991-04-29 | 1992-12-30 | Merck & Co Inc | Multiple hepatitis b virus surface proteins which form particles |
-
1992
- 1992-04-20 IL IL101651A patent/IL101651A0/en unknown
- 1992-04-24 NZ NZ24248792A patent/NZ242487A/en unknown
- 1992-04-27 PL PL92301163A patent/PL171485B1/en unknown
- 1992-04-27 WO PCT/US1992/003472 patent/WO1992019741A1/en not_active Ceased
- 1992-04-27 CA CA002067290A patent/CA2067290A1/en not_active Abandoned
- 1992-04-27 SK SK1181-93A patent/SK118193A3/en unknown
- 1992-04-27 HU HU9303064A patent/HUT69149A/en unknown
- 1992-04-27 CZ CS932271A patent/CZ282769B6/en not_active IP Right Cessation
- 1992-04-28 AU AU15222/92A patent/AU650530B2/en not_active Ceased
- 1992-04-28 CN CN92104290A patent/CN1067680A/en active Pending
- 1992-04-28 YU YU45492A patent/YU45492A/en unknown
- 1992-04-28 KR KR1019920007142A patent/KR920019373A/en not_active Ceased
- 1992-04-28 MX MX9201970A patent/MX9201970A/en unknown
- 1992-04-28 ZA ZA923069A patent/ZA923069B/en unknown
- 1992-04-29 SG SG1996008131A patent/SG55152A1/en unknown
- 1992-04-29 AT AT92303883T patent/ATE215987T1/en active
- 1992-04-29 EP EP92303883A patent/EP0516286B1/en not_active Expired - Lifetime
- 1992-04-29 DE DE69232541T patent/DE69232541D1/en not_active Expired - Lifetime
- 1992-04-30 JP JP4155566A patent/JP2637877B2/en not_active Expired - Fee Related
- 1992-07-01 IE IE138592A patent/IE921385A1/en not_active Application Discontinuation
-
1993
- 1993-03-30 LT LTIP462A patent/LT3367B/en not_active IP Right Cessation
- 1993-10-21 FI FI934650A patent/FI934650A7/en unknown
- 1993-10-28 NO NO933897A patent/NO933897L/en not_active Application Discontinuation
- 1993-10-29 BG BG98187A patent/BG61700B1/en unknown
-
1995
- 1995-01-04 US US08/368,287 patent/US5614384A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| HUT69149A (en) | 1995-08-28 |
| YU45492A (en) | 1994-06-10 |
| MX9201970A (en) | 1992-11-01 |
| IL101651A0 (en) | 1992-12-30 |
| NO933897D0 (en) | 1993-10-28 |
| NZ242487A (en) | 1994-07-26 |
| PL171485B1 (en) | 1997-05-30 |
| FI934650A0 (en) | 1993-10-21 |
| CA2067290A1 (en) | 1992-10-30 |
| BG98187A (en) | 1994-06-30 |
| KR920019373A (en) | 1992-11-19 |
| WO1992019741A1 (en) | 1992-11-12 |
| FI934650A7 (en) | 1993-10-21 |
| NO933897L (en) | 1993-10-28 |
| JPH06141872A (en) | 1994-05-24 |
| EP0516286B1 (en) | 2002-04-10 |
| SK118193A3 (en) | 1994-03-09 |
| JP2637877B2 (en) | 1997-08-06 |
| CZ282769B6 (en) | 1997-10-15 |
| EP0516286A1 (en) | 1992-12-02 |
| LT3367B (en) | 1995-08-25 |
| AU1522292A (en) | 1993-03-11 |
| DE69232541D1 (en) | 2002-05-16 |
| ATE215987T1 (en) | 2002-04-15 |
| CZ227193A3 (en) | 1994-04-13 |
| ZA923069B (en) | 1992-12-30 |
| AU650530B2 (en) | 1994-06-23 |
| BG61700B1 (en) | 1998-03-31 |
| CN1067680A (en) | 1993-01-06 |
| US5614384A (en) | 1997-03-25 |
| HU9303064D0 (en) | 1994-03-28 |
| IE921385A1 (en) | 1992-11-04 |
| SG55152A1 (en) | 1998-12-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TK9A | Rectifications: patents |
Free format text: KNISKERN,PETER J., 841 PATTERSON DRIVE, LANSDALE, PENNSYLVANIA 19446, US, HAGOPIAN,ARPI, 771 HARTLEY DRIVE, LANSDALE, PENNSYLVANIA 19446, US, 19950914 |
|
| MM9A | Lapsed patents |
Effective date: 20040330 |