CA2105277A1 - Genetically engineered vaccine strain - Google Patents

Genetically engineered vaccine strain

Info

Publication number
CA2105277A1
CA2105277A1 CA002105277A CA2105277A CA2105277A1 CA 2105277 A1 CA2105277 A1 CA 2105277A1 CA 002105277 A CA002105277 A CA 002105277A CA 2105277 A CA2105277 A CA 2105277A CA 2105277 A1 CA2105277 A1 CA 2105277A1
Authority
CA
Canada
Prior art keywords
virus
inactivated
recombinant
genetic functions
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002105277A
Other languages
French (fr)
Other versions
CA2105277C (en
Inventor
William I. Cox
Jean-Christophe F. Audonnet
Russell Robert Gettig
Enzo Paoletti
Marion E. Perkus
Jill Taylor
James Tartaglia
Elizabeth K. Norton
Michel Riviere
Charles De Taisne
Keith J. Limbach
Gerard P. Johnson
Steven E. Pincus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Connaught Technology Corp
Original Assignee
William I. Cox
Jean-Christophe F. Audonnet
Russell Robert Gettig
Enzo Paoletti
Marion E. Perkus
Jill Taylor
James Tartaglia
Elizabeth K. Norton
Michel Riviere
Charles De Taisne
Keith J. Limbach
Gerard P. Johnson
Steven E. Pincus
Virogenetics Corporation
Connaught Technology Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William I. Cox, Jean-Christophe F. Audonnet, Russell Robert Gettig, Enzo Paoletti, Marion E. Perkus, Jill Taylor, James Tartaglia, Elizabeth K. Norton, Michel Riviere, Charles De Taisne, Keith J. Limbach, Gerard P. Johnson, Steven E. Pincus, Virogenetics Corporation, Connaught Technology Corporation filed Critical William I. Cox
Priority to CA002558659A priority Critical patent/CA2558659C/en
Priority claimed from PCT/US1992/001906 external-priority patent/WO1992015672A1/en
Publication of CA2105277A1 publication Critical patent/CA2105277A1/en
Application granted granted Critical
Publication of CA2105277C publication Critical patent/CA2105277C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00—Reverse transcribing RNA viruses
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Abstract

What is described is a modified vector, such as a recombinant poxvirus, particularly recombinant vaccinia virus, having enhanced safety. The modified recombinant virus has nonessential virus-encoded genetic functions inactivated therein so that virus has attenuated virulence. In one embodiment, the genetic functions are inactivated by deleting an open reading frame encoding a virulence factor. In another embodiment, the genetic functions are inactivated by insertional inactivation of an open reading frame encoding a virulence factor. What is also described is a vaccine containing the modified recombinant virus having nonessential virus-encoded genetic functions inactivated therein so that the vaccine has an increased level of safety compared to knwon recombinant virus vaccines.
CA002105277A 1991-03-07 1992-03-09 Genetically engineered vaccine strain Expired - Lifetime CA2105277C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002558659A CA2558659C (en) 1991-03-07 1992-03-09 Genetically engineered canary poxvirus-based vaccine strain

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US66605691A 1991-03-07 1991-03-07
US666,056 1991-03-07
US71396791A 1991-06-11 1991-06-11
US713,967 1991-06-11
US84795192A 1992-03-06 1992-03-06
US847,951 1992-03-06
PCT/US1992/001906 WO1992015672A1 (en) 1991-03-07 1992-03-09 Genetically engineered vaccine strain

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002558659A Division CA2558659C (en) 1991-03-07 1992-03-09 Genetically engineered canary poxvirus-based vaccine strain

Publications (2)

Publication Number Publication Date
CA2105277A1 true CA2105277A1 (en) 1992-09-08
CA2105277C CA2105277C (en) 2006-12-12

Family

ID=37560889

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002105277A Expired - Lifetime CA2105277C (en) 1991-03-07 1992-03-09 Genetically engineered vaccine strain

Country Status (1)

Country Link
CA (1) CA2105277C (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329164B2 (en) 2002-08-12 2012-12-11 Jennerex, Inc. Methods and compositions concerning poxviruses and cancer
US9919047B2 (en) 2011-01-04 2018-03-20 Sillajen, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
US9944903B2 (en) 2006-10-16 2018-04-17 Genelux Corporation Modified vaccinia virus strains for use in diagnostic and therapeutic methods
US10463730B2 (en) 2003-06-18 2019-11-05 Genelux Corporation Microorganisms for therapy
US11149254B2 (en) 2011-04-15 2021-10-19 Genelux Corporation Clonal strains of attenuated vaccinia viruses and methods of use thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007030668A2 (en) 2005-09-07 2007-03-15 Jennerex Biotherapeutics Ulc Systemic treatment of metastatic and/or systemically-disseminated cancers using gm-csf-expressing poxviruses
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
HUE038708T2 (en) 2009-09-14 2018-11-28 Sillajen Biotherapeutics Inc Oncolytic vaccinia virus combination cancer therapy

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8329164B2 (en) 2002-08-12 2012-12-11 Jennerex, Inc. Methods and compositions concerning poxviruses and cancer
US10463730B2 (en) 2003-06-18 2019-11-05 Genelux Corporation Microorganisms for therapy
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