YU30495A - PROCEDURE FOR OBTAINING HBV SURFACE PROTEINS - Google Patents
PROCEDURE FOR OBTAINING HBV SURFACE PROTEINSInfo
- Publication number
- YU30495A YU30495A YU30495A YU30495A YU30495A YU 30495 A YU30495 A YU 30495A YU 30495 A YU30495 A YU 30495A YU 30495 A YU30495 A YU 30495A YU 30495 A YU30495 A YU 30495A
- Authority
- YU
- Yugoslavia
- Prior art keywords
- hbv
- surface proteins
- yeast strains
- procedure
- particles
- Prior art date
Links
- 102000018697 Membrane Proteins Human genes 0.000 title abstract 4
- 108010052285 Membrane Proteins Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 241000700721 Hepatitis B virus Species 0.000 abstract 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 3
- 239000002245 particle Substances 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000002950 deficient Effects 0.000 abstract 1
- 239000007857 degradation product Substances 0.000 abstract 1
- 230000006806 disease prevention Effects 0.000 abstract 1
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000017854 proteolysis Effects 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2730/00—Reverse transcribing DNA viruses
- C12N2730/00011—Details
- C12N2730/10011—Hepadnaviridae
- C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
- C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
POSTUPAK ZA DOBIJANJE HBV POVRŠINSKIH PROTEIN A - U cilju da se dobiju površinski proteini virusa hepatitisa B (HBV) u obliku čestica sa znatno smanjenim sadržajem zarobljenog ugljenog hidrata, DNK koja kodira HBV površinske proteine se ekspresioniše u rekombinatnim sojevima kvasca koji su deficijentni u njihovoj sposobnosti da glikoziluju proteine i/ili imaju mutacije u genima proteaze. Ovi sojevi kvasća proizvode P24 sa znatno smanjenim sub-P24 proteolitičkim proizvodima razaranja u poređenju sa P24 proižvedenim u "divljeg tipa" ćelijama kvasca. Ove čestice su korisne kao vakcina kako za aktivno tako i za pasivno tretiranje ili prevenciju bolesti i/ili infekcije izazvane" sa HBV ili drugim agensima serološki srodnim sa HBV. Postupci opisani ovde koristeći ove sojeve kvasca proizvode efikasnije P24.PROCEDURE FOR OBTAINING HBV SURFACE PROTEINS A - In order to obtain hepatitis B virus (HBV) surface proteins in the form of particles with a significantly reduced content of trapped carbohydrates, the DNA encoding HBV surface proteins is expressed in recombinant yeast strains that are deficient in their ability to glycosylate proteins and/or have mutations in protease genes. These yeast strains produce P24 with greatly reduced sub-P24 proteolytic degradation products compared to P24 produced in "wild-type" yeast cells. These particles are useful as a vaccine for both active and passive treatment or prevention of disease and/or infection caused by HBV or other agents serologically related to HBV. The methods described herein using these yeast strains produce more efficient P24.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24280094A | 1994-05-16 | 1994-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| YU30495A true YU30495A (en) | 1997-12-05 |
Family
ID=22916238
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| YU30495A YU30495A (en) | 1994-05-16 | 1995-05-12 | PROCEDURE FOR OBTAINING HBV SURFACE PROTEINS |
Country Status (4)
| Country | Link |
|---|---|
| AU (1) | AU2515095A (en) |
| HR (1) | HRP950289A2 (en) |
| WO (1) | WO1995031558A1 (en) |
| YU (1) | YU30495A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104628830A (en) * | 2015-01-23 | 2015-05-20 | 深圳康泰生物制品股份有限公司 | HBsAg, technology for preparing HBsAg through expression by virtue of recombinant saccharymyces cerevisiae, separation and purification technology for HBsAg as well as hepatitis B vaccine |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5135854A (en) * | 1987-10-29 | 1992-08-04 | Zymogenetics, Inc. | Methods of regulating protein glycosylation |
| CA2067003A1 (en) * | 1991-04-29 | 1992-10-30 | Peter J. Kniskern | Hbsag escape mutant vaccine |
| IL101651A0 (en) * | 1991-04-29 | 1992-12-30 | Merck & Co Inc | Hepatitis b virus surface proteins with reduced host carbohydrate content |
-
1995
- 1995-05-12 WO PCT/US1995/006061 patent/WO1995031558A1/en active Application Filing
- 1995-05-12 AU AU25150/95A patent/AU2515095A/en not_active Abandoned
- 1995-05-12 YU YU30495A patent/YU30495A/en unknown
- 1995-05-15 HR HR08/242,800A patent/HRP950289A2/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2515095A (en) | 1995-12-05 |
| HRP950289A2 (en) | 1997-04-30 |
| WO1995031558A1 (en) | 1995-11-23 |
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