LT3790993T - Dvigrandžių lūžių vietų identifikavimo ir apibūdinimo būdai ir jų panaudojimas - Google Patents

Dvigrandžių lūžių vietų identifikavimo ir apibūdinimo būdai ir jų panaudojimas

Info

Publication number
LT3790993T
LT3790993T LTEPPCT/US2019/031719T LTUS2019031719T LT3790993T LT 3790993 T LT3790993 T LT 3790993T LT US2019031719 T LTUS2019031719 T LT US2019031719T LT 3790993 T LT3790993 T LT 3790993T
Authority
LT
Lithuania
Prior art keywords
characterization
identification
double
methods
application
Prior art date
Application number
LTEPPCT/US2019/031719T
Other languages
English (en)
Lithuanian (lt)
Inventor
Stephane Deschamps
Virginijus SIKSNYS
Joshua K. Young
Mindaugas ZAREMBA
Original Assignee
Pioneer Hi-Bred International, Inc.
Vilnius University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc., Vilnius University filed Critical Pioneer Hi-Bred International, Inc.
Publication of LT3790993T publication Critical patent/LT3790993T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/525—Phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LTEPPCT/US2019/031719T 2018-05-11 2019-05-10 Dvigrandžių lūžių vietų identifikavimo ir apibūdinimo būdai ir jų panaudojimas LT3790993T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862670366P 2018-05-11 2018-05-11
PCT/US2019/031719 WO2019217816A1 (en) 2018-05-11 2019-05-10 Methods for the identification and characterization of double-strand break sites and compositions and uses thereof

Publications (1)

Publication Number Publication Date
LT3790993T true LT3790993T (lt) 2026-04-10

Family

ID=68467567

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEPPCT/US2019/031719T LT3790993T (lt) 2018-05-11 2019-05-10 Dvigrandžių lūžių vietų identifikavimo ir apibūdinimo būdai ir jų panaudojimas

Country Status (5)

Country Link
US (2) US12065689B2 (de)
EP (1) EP3790993B1 (de)
CA (1) CA3099065A1 (de)
LT (1) LT3790993T (de)
WO (1) WO2019217816A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3221516A1 (en) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
CN107653256A (zh) * 2017-11-21 2018-02-02 云南省烟草农业科学研究院 一种烟草多酚氧化酶基因NtPPO1及其定点突变方法与应用
EP3755792A4 (de) 2018-02-23 2021-12-08 Pioneer Hi-Bred International, Inc. Neue cas9-orthologe
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
GB202013141D0 (en) 2020-08-21 2020-10-07 Univ College Cardiff Consultants Ltd A screening method for the detection of DNA damage
WO2024033378A1 (en) * 2022-08-09 2024-02-15 Institute Of Molecular Biology Ggmbh (Imb) Method of parallel, rapid and sensitive detection of dna double strand breaks
EP4321630A1 (de) * 2022-08-09 2024-02-14 Institute of Molecular Biology gGmbH (IMB) Verfahren zur parallelen, schnellen und empfindlichen detektion von dna-doppelstrangbrüchen
CN121532509A (zh) * 2023-05-09 2026-02-13 正基基因科技有限公司 用于鉴定核酸酶的序列邻近基序、效力及特异性的方法与系统
EP4471159B1 (de) 2023-05-31 2025-11-12 Vilnius University Detektion von crispr-nuklease-off-target durch sequenzierung (croft-seq)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA123532C2 (uk) * 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
DK3604544T3 (en) * 2014-01-31 2022-02-14 Swift Biosciences Inc Improved methods for processing dna substrates
AU2015270774B2 (en) 2014-06-05 2021-10-14 Qiagen Gmbh Optimization of DNA amplification reactions
EP3294878A1 (de) * 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Guide-rns/cas-endonuklease-systeme
US11046995B2 (en) 2016-08-16 2021-06-29 The Regents Of The University Of California Method for finding low abundance sequences by hybridization (FLASH)

Also Published As

Publication number Publication date
US12065689B2 (en) 2024-08-20
WO2019217816A1 (en) 2019-11-14
CA3099065A1 (en) 2019-11-14
US20250188512A1 (en) 2025-06-12
EP3790993A1 (de) 2021-03-17
EP3790993A4 (de) 2022-02-16
EP3790993B1 (de) 2025-12-03
US20210147909A1 (en) 2021-05-20

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