LT3183340T - Termolabilios egzonukleazės - Google Patents

Termolabilios egzonukleazės

Info

Publication number
LT3183340T
LT3183340T LT15770444T LT15770444T LT3183340T LT 3183340 T LT3183340 T LT 3183340T LT 15770444 T LT15770444 T LT 15770444T LT 15770444 T LT15770444 T LT 15770444T LT 3183340 T LT3183340 T LT 3183340T
Authority
LT
Lithuania
Prior art keywords
thermolabile
exonucleases
thermolabile exonucleases
Prior art date
Application number
LT15770444T
Other languages
English (en)
Inventor
Terese Solstad
Elisabeth Andreassen
Marit Lorentzen
Olav Lanes
Morten Elde
Atle Larsen
Yvonne Piotrowski
Nils Willassen
Original Assignee
Arcticzymes As
Univ I Tromso Norges Arktiske Univ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arcticzymes As, Univ I Tromso Norges Arktiske Univ filed Critical Arcticzymes As
Publication of LT3183340T publication Critical patent/LT3183340T/lt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/11Exodeoxyribonucleases producing 5'-phosphomonoesters (3.1.11)
    • C12Y301/11001Exodeoxyribonuclease I (3.1.11.1)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/30Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/325Single stranded exonuclease
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
LT15770444T 2014-08-19 2015-08-19 Termolabilios egzonukleazės LT3183340T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1414745.8A GB201414745D0 (en) 2014-08-19 2014-08-19 Exonucleases
PCT/EP2015/001703 WO2016026574A1 (en) 2014-08-19 2015-08-19 Thermolabile exonucleases

Publications (1)

Publication Number Publication Date
LT3183340T true LT3183340T (lt) 2019-10-25

Family

ID=51662672

Family Applications (1)

Application Number Title Priority Date Filing Date
LT15770444T LT3183340T (lt) 2014-08-19 2015-08-19 Termolabilios egzonukleazės

Country Status (12)

Country Link
US (3) US10087483B2 (lt)
EP (1) EP3183340B1 (lt)
JP (1) JP6316505B2 (lt)
KR (2) KR20170040263A (lt)
CN (1) CN107109383B (lt)
CA (1) CA2956853C (lt)
DK (1) DK3183340T3 (lt)
ES (1) ES2742186T3 (lt)
GB (1) GB201414745D0 (lt)
LT (1) LT3183340T (lt)
PL (1) PL3183340T3 (lt)
WO (1) WO2016026574A1 (lt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11578332B2 (en) * 2016-07-14 2023-02-14 Gene Infinity Llc Methods of rapid ligation-independent cloning of DNA and uses thereof
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
GB201721053D0 (en) * 2017-12-15 2018-01-31 Univ I Tromsø - Norges Arktiske Univ DNA polymerases
WO2020172362A1 (en) * 2019-02-20 2020-08-27 Genetics Research, Llc Regulated multiplex reactions in a single tube
EP4041878A1 (en) 2019-10-01 2022-08-17 Universitetet i Tromsø - Norges Arktiske Universitet Marine dna polymerase i
WO2021064106A1 (en) 2019-10-01 2021-04-08 Universitetet I Tromsø - Norges Arktiske Universitet Dna polymerase and dna polymerase derived 3'-5'exonuclease
WO2022040557A2 (en) * 2020-08-21 2022-02-24 Ultima Genomics, Inc. Compositions for surface amplification and uses thereof
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
EP4414460A1 (en) 2023-02-07 2024-08-14 Arcticzymes AS Compositions comprising a sequence specific endoribonuclease and methods of use

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
CA2101119A1 (en) * 1992-08-17 1994-02-18 George T. Walker Exonuclease decontamination method
CA2137728C (en) 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
CA2168712A1 (en) 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
ATE236270T1 (de) * 1995-12-15 2003-04-15 Univ Duke Verfahren zum nachweis und zur entfernung von mutantsequenzen, die während enzymatischer amplifikation entstehen
GB9918150D0 (en) 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
US6379940B2 (en) 2000-03-21 2002-04-30 Usb Corporation Stable composition comprising a nuclease and a phosphatase
US8361712B2 (en) 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US20030087278A1 (en) 2001-08-15 2003-05-08 Jon Sayers Nuclease assay
EP1444354B1 (en) 2001-11-13 2007-01-31 Serono Genetics Institute S.A. Method for enhancing the efficiency of an enzymatic purification process
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro
EP1534859B1 (en) * 2002-06-28 2010-11-24 PrimeraDx, Inc. Methods of detecting sequence differences
JP3959315B2 (ja) 2002-07-26 2007-08-15 株式会社東芝 核酸汚染防止方法、それにおいて使用される抱埋化プライマーおよびそれを具備するキット
US20040126765A1 (en) 2002-12-27 2004-07-01 Adams Craig W. Method and compositions for sequencing nucleic acid molecules
US20070122811A1 (en) 2003-09-30 2007-05-31 Philip Buzby Compositions and processes for genotyping single nucleotide polymorphisms
US8034597B2 (en) 2005-03-08 2011-10-11 Takara Bio Inc. Microorganism-derived psychrophilic endonuclease
US20080044851A1 (en) 2006-06-02 2008-02-21 Epicentre Technologies Compositions and methods for removal of DNA from a sample
WO2009100080A1 (en) 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US9249457B2 (en) 2008-06-18 2016-02-02 Riken Method of manufacturing a mixture of amplified double-stranded nucleic acids comprising unknown sequence
US9234226B2 (en) 2008-10-14 2016-01-12 Wayne M. Barnes In vitro recombination methods using 5′ exonuclease
EP2391655B1 (en) 2009-01-30 2017-10-11 Oxford Nanopore Technologies Limited Hybridization linkers
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
GB201120494D0 (en) 2011-11-29 2012-01-11 Univ Sheffield Modified exonuclease
EP2814954B1 (en) 2012-02-17 2017-04-05 Biotec Pharmacon ASA Endonucleases

Also Published As

Publication number Publication date
PL3183340T3 (pl) 2019-12-31
DK3183340T3 (da) 2019-08-19
US20200332352A9 (en) 2020-10-22
US10087483B2 (en) 2018-10-02
CN107109383B (zh) 2019-04-02
KR20170040263A (ko) 2017-04-12
JP2017523803A (ja) 2017-08-24
WO2016026574A1 (en) 2016-02-25
GB201414745D0 (en) 2014-10-01
KR102432501B1 (ko) 2022-08-12
US20200071753A1 (en) 2020-03-05
US10787702B2 (en) 2020-09-29
CA2956853C (en) 2022-05-17
US20180363042A1 (en) 2018-12-20
ES2742186T3 (es) 2020-02-13
JP6316505B2 (ja) 2018-04-25
CA2956853A1 (en) 2016-02-25
US10415082B2 (en) 2019-09-17
CN107109383A (zh) 2017-08-29
EP3183340A1 (en) 2017-06-28
KR20180040736A (ko) 2018-04-20
US20170233800A1 (en) 2017-08-17
EP3183340B1 (en) 2019-05-15

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