LT2004109A - Grandines specifiškai nikuojančių endonukleazių konstravimo iš restrikcijos endonukleazių būdas - Google Patents
Grandines specifiškai nikuojančių endonukleazių konstravimo iš restrikcijos endonukleazių būdasInfo
- Publication number
- LT2004109A LT2004109A LT2004109A LT2004109A LT2004109A LT 2004109 A LT2004109 A LT 2004109A LT 2004109 A LT2004109 A LT 2004109A LT 2004109 A LT2004109 A LT 2004109A LT 2004109 A LT2004109 A LT 2004109A
- Authority
- LT
- Lithuania
- Prior art keywords
- gene
- endonuclease
- product
- restriction
- nicking
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 101710147059 Nicking endonuclease Proteins 0.000 title 1
- 239000013612 plasmid Substances 0.000 abstract 5
- 102000004533 Endonucleases Human genes 0.000 abstract 3
- 108010042407 Endonucleases Proteins 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 1
- 238000000099 in vitro assay Methods 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Būdai, skirti konstravimui naujų grandinei specifinių nikuojančių endonukleazių, pasinaudojant plazmidžių bibliotekos, turinčios atsitiktinai mutagenizuotą restrikcijos endonukleazės geną, praturtinimu in vivo. Plazmidės turi gretimą genui skaldomą arba nikuojamą seką, skaldymui arba nikavimui geno endonukleazės produktu ir antrąją atpažinimo vietą antrajai endonukleazei. Plazmidžių biblioteka naudojama nemodifikuotų ląstelių-šeimininkių transformavimui. Plazmidės iš išaugintų transformuotų ląstelių gali būti analizuojamos in vitro būdu dėl nikavimo, nikuotos plazmidės surenkamos ir naudojamos transformuoti ląsteles-šeimininkes. Produktas po to surenkamas ir nustatomas endonukleazės viengrandis specifiškumas. Produktas arba klonuojamas po amplifikacijos, arba identifikuojamas, naudojant selektyvų markerį. ą
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53075403P | 2003-12-18 | 2003-12-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LT2004109A true LT2004109A (lt) | 2005-06-27 |
| LT5263B LT5263B (lt) | 2005-09-26 |
Family
ID=34633011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LT2004109A LT5263B (lt) | 2003-12-18 | 2004-12-17 | Grandines specifiškai nikuojančių endonukleazių konstravimo iš restrikcijos endonukleazių būdas |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US7943303B2 (lt) |
| LT (1) | LT5263B (lt) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004067764A2 (en) * | 2003-01-29 | 2004-08-12 | Keck Graduate Institute | Nucleic acid sequencing using nicking agents |
| WO2008127901A1 (en) * | 2007-04-13 | 2008-10-23 | President And Fellows Of Harvard College | Region-specific hyperbranched amplification |
| EP2392653B1 (en) | 2007-07-12 | 2014-03-19 | New England Biolabs, Inc. | High fidelity restriction endonucleases |
| US11198910B2 (en) | 2016-09-02 | 2021-12-14 | New England Biolabs, Inc. | Analysis of chromatin using a nicking enzyme |
| EP3710596B1 (en) | 2017-11-16 | 2023-08-02 | New England Biolabs, Inc. | Mapping the location, type and strand of damaged nucleotides in double-stranded dna |
| US11021701B2 (en) | 2017-11-22 | 2021-06-01 | New England Biolabs, Inc. | Method for fragmenting DNA by nick translation |
| EP3999646A4 (en) * | 2019-07-17 | 2023-08-30 | Generation Bio Co. | COMPOSITIONS AND PRODUCTION OF NICKEL-CLOSED CLOSED-END DNA VECTOR |
| AU2020315444A1 (en) | 2019-07-17 | 2021-12-23 | Generation Bio Co. | Synthetic production of single-stranded adeno associated viral DNA vectors |
| US20230123635A1 (en) | 2020-06-12 | 2023-04-20 | New England Biolabs, Inc. | Compositions and Methods for Detecting Molecular Targets on Chromosomal DNA |
| CN116234917A (zh) | 2020-07-27 | 2023-06-06 | 安杰瑞姆生物科学公司 | Dna分子组合物及其制备方法和使用方法 |
| AU2022260111A1 (en) | 2021-04-20 | 2023-11-30 | Anjarium Biosciences Ag | Compositions of dna molecules encoding amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase, methods of making thereof, and methods of use thereof |
| US11667968B2 (en) | 2021-05-27 | 2023-06-06 | New England Biolabs, Inc. | Fragmentation of DNA |
| WO2022261281A1 (en) | 2021-06-09 | 2022-12-15 | New England Biolabs, Inc. | Target initiation and amplification of long dna with nuclease and replisome enzymes |
| JP2025504404A (ja) | 2022-01-14 | 2025-02-12 | アンジャリウム バイオサイエンシズ エージー | Ｖｉｉｉ因子をコードするｄｎａ分子の組成物、それを作製する方法、及びその使用方法 |
| EP4665865A1 (en) | 2023-02-17 | 2025-12-24 | Anjarium Biosciences AG | Methods of making dna molecules and compositions and uses thereof |
| WO2025104680A1 (en) | 2023-11-17 | 2025-05-22 | Friedrich Miescher Institute For Biomedical Research | Dna accessibility for the assessment of male fertility |
| CN118879682A (zh) * | 2024-07-16 | 2024-11-01 | 上海交通大学 | 一种制备长单链dna的方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0018120D0 (en) | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
| US6660475B2 (en) * | 2000-12-15 | 2003-12-09 | New England Biolabs, Inc. | Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof |
| US6395523B1 (en) | 2001-06-01 | 2002-05-28 | New England Biolabs, Inc. | Engineering nicking endonucleases from type IIs restriction endonucleases |
| AU2002365122A1 (en) | 2001-08-23 | 2003-07-15 | New England Biolabs, Inc. | A method for engineering strand-specific, sequence-specific, dna-nicking enzymes |
-
2004
- 2004-12-15 US US11/013,260 patent/US7943303B2/en not_active Expired - Lifetime
- 2004-12-17 LT LT2004109A patent/LT5263B/lt not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US7943303B2 (en) | 2011-05-17 |
| US20050158834A1 (en) | 2005-07-21 |
| LT5263B (lt) | 2005-09-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| TK9A | Rectifications: patents | ||
| MM9A | Lapsed patents |
Effective date: 20221217 |