KR980000469A - Immunization method using chimeric poliovirus vaccine vector expressing HBV antigen protein - Google Patents

Immunization method using chimeric poliovirus vaccine vector expressing HBV antigen protein Download PDF

Info

Publication number
KR980000469A
KR980000469A KR1019960023416A KR19960023416A KR980000469A KR 980000469 A KR980000469 A KR 980000469A KR 1019960023416 A KR1019960023416 A KR 1019960023416A KR 19960023416 A KR19960023416 A KR 19960023416A KR 980000469 A KR980000469 A KR 980000469A
Authority
KR
South Korea
Prior art keywords
hbv
vaccine vector
chimeric
surface antigen
antigen protein
Prior art date
Application number
KR1019960023416A
Other languages
Korean (ko)
Other versions
KR100189699B1 (en
Inventor
이범용
민미경
문홍모
Original Assignee
허영섭
재단법인 목암생명공학연구소
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 허영섭, 재단법인 목암생명공학연구소 filed Critical 허영섭
Priority to KR1019960023416A priority Critical patent/KR100189699B1/en
Publication of KR980000469A publication Critical patent/KR980000469A/en
Application granted granted Critical
Publication of KR100189699B1 publication Critical patent/KR100189699B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/125—Picornaviridae, e.g. calicivirus
    • A61K39/13—Poliovirus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/085—Picornaviridae, e.g. coxsackie virus, echovirus, enterovirus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Virology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Genetics & Genomics (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 플리오바이러스의 전체 cDNA 유전자 중에서 VP2 및 VP3 코딩부위가 제거된 위치에 2A 올리고펩타이드 코딩 유전자 및 HBV의 표면항원 단백질 유전자를 가지며, HBV의 표면항원 단백질을 발현하는 키메라 폴리오-HBV 백신벡터 및 그를 이용한 포유동물의 면역화 방법을 제공한다. 본 발명의 백신벡터는 폴리오바이러스 백신의 많은 장점을 보존하고 있는 폴리오-HBV 키메라 바이러스를 생산하며, 이 때 HBV의 표면항원 단백질이 지속적으로 발현됨을 확인하였다. 또한, 전기 폴리오-HBV 키메라 바이러스를 포유동물에 투여하면, HBV 표면항원 단백질에 특이적인 체액성 또는 세포성 면역이 유도되어, HBV의 감염으로부터 동물을 면역화시킬 수 있다.The present invention provides a chimeric folio-HBV vaccine vector having a 2A oligopeptide coding gene and a HBV surface antigen protein gene at a position where VP2 and VP3 coding regions are removed from all cDNA genes of plioviruses. And a mammalian immunization method using the same. The vaccine vector of the present invention produces polio-HBV chimeric virus, which preserves many of the advantages of poliovirus vaccine, and it was confirmed that surface antigen proteins of HBV were continuously expressed. In addition, administration of the polio-HBV chimeric virus to mammals induces humoral or cellular immunity specific for HBV surface antigen proteins, thereby immunizing animals from infection with HBV.

Description

HBV 항원단백질을 발현하는 키메라 폴리오바이러스 백신벡터를 이용한 면역화 방법Immunization method using chimeric poliovirus vaccine vector expressing HBV antigen protein

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (3)

폴리오바이러스의 전체 cDNA 유전자 중에서 VP2 및 VP3 코딩부위가 제거된 위치에 2A 올리고펩타이드코딩 유전자 및 HBV의 표면 항원 단백질 유전자를 가지며, HBV의 항원단백질을 발현하는 키메라 폴리오-HBV 벡신벡터 pPS(KFCC-10905).Chimeric Folio-HBV Bexin vector pPS having 2A oligopeptide coding gene and HBV surface antigen protein gene at the position where the VP2 and VP3 coding sites were removed from all cDNA genes of poliovirus and expressing the antigenic protein of HBV (KFCC-10905) ). 제1항의 키메라 폴리오-HBV 백신벡터 pPS(KFCC-10905)로부터 발현되는 HBV의 표면항원 단백질.The surface antigen protein of HBV expressed from the chimeric polio-HBV vaccine vector pPS (KFCC-10905) of Claim 1. 제1항의 키메라 폴리오-HBV 백신벡터 pPS(KFCC-10905)를 포유동물에 투여하고 체액성 또는 세포성 면역을 유도하여, HBV의 감염으로부터 동물을 면역화시키는 방법.A method of immunizing an animal from infection of HBV by administering the chimeric polio-HBV vaccine vector pPS (KFCC-10905) of claim 1 to the mammal and inducing humoral or cellular immunity. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960023416A 1996-06-25 1996-06-25 Immunization method using chimeric poliovirus vaccine vector expressing hbv antigen proteins KR100189699B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960023416A KR100189699B1 (en) 1996-06-25 1996-06-25 Immunization method using chimeric poliovirus vaccine vector expressing hbv antigen proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960023416A KR100189699B1 (en) 1996-06-25 1996-06-25 Immunization method using chimeric poliovirus vaccine vector expressing hbv antigen proteins

Publications (2)

Publication Number Publication Date
KR980000469A true KR980000469A (en) 1998-03-30
KR100189699B1 KR100189699B1 (en) 1999-06-01

Family

ID=19463178

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019960023416A KR100189699B1 (en) 1996-06-25 1996-06-25 Immunization method using chimeric poliovirus vaccine vector expressing hbv antigen proteins

Country Status (1)

Country Link
KR (1) KR100189699B1 (en)

Also Published As

Publication number Publication date
KR100189699B1 (en) 1999-06-01

Similar Documents

Publication Publication Date Title
DE69333397D1 (en) RETRO, INVERSO, AND RETRO INVERSO SYNTHETIC PEPTIDE ANALOGS
EA200100425A1 (en) NEW METHODS OF THERAPEUTIC VACCINATION
DE69327587D1 (en) Conjugates of weakly immunogenic antigenic and synthetic peptide carriers and vaccines containing them
EA199800973A1 (en) POLYPEPTID BELKA-106 OF THE EXTERNAL MORAXELLA CATARRHALIS MEMBRANE, SEQUENCE OF THE GENE AND THEIR APPLICATION
DE60228758D1 (en) IMMUNIZATION AGAINST CHLAMYDIA TRACHEOMATIS
ES2138784T3 (en) SYNTHETIC ANTIGENS FOR THE DETECTION OF ANTIBODIES AGAINST THE HEPATITIS C VIRUS.
FI913560A (en) SYNTHETIC PEPTIDER, SPECIELLT FOER OBSERVATION AV HCV-ANTI-KROPPAR, HCV-INFEKTIONSDIAGNOS OCH DESS FOERHINDRANDE SM VACCIN.
ATE125821T1 (en) VACCINE AGAINST HEPATITIS B.
BR9708401A (en) Para-smallpox Virus Vectors
KR940703851A (en) Hepatitis E Virus Peptide Antigen and Antibody (HEPATITIS E VIRUS PEPTIDE ANTIGENS AND ANTIBODIES)
FI834590A (en) SYNTHETIC PICORNAVIRUS ANTIGEN.
KR910019641A (en) Serum Reactive Epitopes on Proteins of Human Papilloma Virus (HPV) 18
KR890700030A (en) Vaccines and diagnostic methods for influenza (Haemophilus influenzae)
RU99108121A (en) IMMUNOGENOUS PEPTIDES OF FMD VIRUSES
EP0967223A3 (en) Hepatitis C virus peptide antigens and a method of testing for the hepatitis C virus (HCV)
KR900701291A (en) Vaccines and diagnostic tests for hemophilus influenza
KR980000469A (en) Immunization method using chimeric poliovirus vaccine vector expressing HBV antigen protein
DE69301733D1 (en) Vaccines against metazoan parasites
DE68926274D1 (en) Recombinant HBsAg hybrid particles which have morphological properties of HBsAg antigen and which contain an immunogenic sequence which induces neutralizing antibodies against HIV or which is recognized by these antibodies, nucleotide sequences which encode these particles and vaccines containing them
ATE71534T1 (en) VACCINE.
FR2724385B1 (en) FELINE INFECTIOUS PERITONITIS VACCINE.
DE69132717T2 (en) HEPATITIS A VACCINE
ATE143373T1 (en) PROTEINS, VACCINES AND NUCLIC ACIDS
RU92004487A (en) DNA, POLIPEPTID, VACCINE, VECTOR, MICROORGANISM, INSECTED CELL, REAGENT, METHODS OF TREATMENT AND PREVENTION, METHOD OF DETECTING ANTIGENES

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20020115

Year of fee payment: 6

LAPS Lapse due to unpaid annual fee